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The map label for this gene is focA [H]

Identifier: 59713996

GI number: 59713996

Start: 919440

End: 920288

Strand: Reverse

Name: focA [H]

Synonym: VF_A0813

Alternate gene names: 59713996

Gene position: 920288-919440 (Counterclockwise)

Preceding gene: 59713997

Following gene: 59713993

Centisome position: 69.18

GC content: 39.1

Gene sequence:

>849_bases
ATGAACCAATTCGATTCTTTACTACCGCCACAAATGGCTGAACGCGCTGCTGAAGTTGGCGTAGCAAAAGCAACTAAAGA
TCCATTTAAATCTTTTTTATTAGCAATTACTGCAGGCATACATATCGGTATTGCATTTATCTTCTATACCACAGTAACAA
CTGGTGCTGAATCAATGCCATGGGGTATGTCCCATTTCGTTGGTGGTTTGGCTTTCAGTCTTGGTCTTATTTTAGTTGTG
GTAACTGGTGGTGAACTATTTACCAGCTCGGTGTTAACTTTAGTTGCTCGTGCAAGTGGTAAAATCAGCTGGAAAAACCT
ATGTGCGAACTGGGCCGTTGTTTATGTTGGTAACTTTATAGGTGCCATGCTACTTGTTGGTATCATGTTAGTTGCAAAAC
AATACATGAATGACGGTGGTACCGTTGGTATCAACACAATGAAAATTGCTCAACATAAGTTGCATCATGGTTTCTGGCAG
GCGATTGCGTTAGGTCTTATGTGTAACGTATTGGTTTGTGTTGGTGTATGGATGACATTTTCTGGTCGTACATTAACAGA
TAAAATTTTGGTTATGATCTTACCTGTAGCAATGTTTGTATCAGCAGGTTTTGAGCACTGTATTGCGAATATGTTCCAAA
TCCCAATGGCGATTGGCATTAAAACTTTTGCTTCTCCTGAGTTCTGGGCTGCAAGTGGTTATTCAGCATTGGACTTTGCA
GATTTAACATTACATAATTTTGTGATTAATAACCTTATCCCAGTTACGATTGGTAATATTATTGGTGGTGGCATCTTCGT
TGGTATGGGTTACTGGATTATTTATCTTCGTGAACCAAATCATCACTAA

Upstream 100 bases:

>100_bases
TCTTAAAATTAATACTCTTCAACAATAAAATTGCTTGTATTCCGAACAAAATAGGAGGATTATCCGACCTCCTATTCACT
TGTATCAAAATGTTTCTACT

Downstream 100 bases:

>100_bases
TAAATATCAGTAGTATTACAGATGTAAAAAAAGGAGCATATTGAATGCTCCTTTTTTGTATCTATTGATTTACCAGTTTA
CACCAATCAATTACGATTCT

Product: formate transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MNQFDSLLPPQMAERAAEVGVAKATKDPFKSFLLAITAGIHIGIAFIFYTTVTTGAESMPWGMSHFVGGLAFSLGLILVV
VTGGELFTSSVLTLVARASGKISWKNLCANWAVVYVGNFIGAMLLVGIMLVAKQYMNDGGTVGINTMKIAQHKLHHGFWQ
AIALGLMCNVLVCVGVWMTFSGRTLTDKILVMILPVAMFVSAGFEHCIANMFQIPMAIGIKTFASPEFWAASGYSALDFA
DLTLHNFVINNLIPVTIGNIIGGGIFVGMGYWIIYLREPNHH

Sequences:

>Translated_282_residues
MNQFDSLLPPQMAERAAEVGVAKATKDPFKSFLLAITAGIHIGIAFIFYTTVTTGAESMPWGMSHFVGGLAFSLGLILVV
VTGGELFTSSVLTLVARASGKISWKNLCANWAVVYVGNFIGAMLLVGIMLVAKQYMNDGGTVGINTMKIAQHKLHHGFWQ
AIALGLMCNVLVCVGVWMTFSGRTLTDKILVMILPVAMFVSAGFEHCIANMFQIPMAIGIKTFASPEFWAASGYSALDFA
DLTLHNFVINNLIPVTIGNIIGGGIFVGMGYWIIYLREPNHH
>Mature_282_residues
MNQFDSLLPPQMAERAAEVGVAKATKDPFKSFLLAITAGIHIGIAFIFYTTVTTGAESMPWGMSHFVGGLAFSLGLILVV
VTGGELFTSSVLTLVARASGKISWKNLCANWAVVYVGNFIGAMLLVGIMLVAKQYMNDGGTVGINTMKIAQHKLHHGFWQ
AIALGLMCNVLVCVGVWMTFSGRTLTDKILVMILPVAMFVSAGFEHCIANMFQIPMAIGIKTFASPEFWAASGYSALDFA
DLTLHNFVINNLIPVTIGNIIGGGIFVGMGYWIIYLREPNHH

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=281, Percent_Identity=58.3629893238434, Blast_Score=345, Evalue=1e-96,
Organism=Escherichia coli, GI1788837, Length=276, Percent_Identity=42.3913043478261, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI87082249, Length=267, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321779, Length=224, Percent_Identity=24.1071428571429, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 30455; Mature: 30455

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS01005 FORMATE_NITRITE_TP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
>Mature Secondary Structure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HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]