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The map label for this gene is pntB [H]

Identifier: 59713768

GI number: 59713768

Start: 668217

End: 669593

Strand: Reverse

Name: pntB [H]

Synonym: VF_A0585

Alternate gene names: 59713768

Gene position: 669593-668217 (Counterclockwise)

Preceding gene: 59713769

Following gene: 59713767

Centisome position: 50.33

GC content: 43.36

Gene sequence:

>1377_bases
ATGTCAGCAGGATTAGTTCAAGCAGCGTATATTGTATCTGCCGTTCTATTTATCATGAGTTTAGCAGGTCTGTCTAAACA
AGAGACAGCTCGTAGCGGTAACTACTACGGTATTACTGGTATGGCTATCGCACTTATCGCAACCATCTTTGGCCCTGATT
CAACAGGGACAGGCTGGATCATCGTAGCAATGGCTATGGGTGGCGCGATTGGTCTTTACTATGCGAAAAAAGTTGAAATG
ACTGAAATGCCAGAACTTGTGGCAATTCTTCACAGCTTTGTTGGTATGGCTGCGGTTCTTGTTGGTTTCAACAGTTACAT
CGACCACGGTGTTCTTGTTGGTGCTATGCTAAATATCCACCTTGTAGAGGTATTCTTAGGCGTCTTCATTGGTGCGGTTA
CTTTCACAGGTTCTTTAGTGGCATTCGGTAAACTACGTGGAGTGATTAACTCATCTGCACTTCAATTACCTCACCGTCAT
AAGCTGAACTTACTAGCATTAGTTGTATCTGCAGGTCTTCTTTACCACTTCGTAAGTGTTGAAGGCAGCATGTTCGCACT
TATTCTAGTAACACTGATCGCATTTGCATTCGGTTATCACTTGGTTGCTTCAATCGGTGGTGCGGATATGCCAGTAGTTG
TTTCAATGCTTAACTCATACTCTGGTTGGGCAGCGGCAGCGGCAGGTTTCATGCTATCAAACGATCTACTGATTGTTACT
GGTGCATTAGTAGGTTCATCAGGTGCAATTCTGTCTTACATCATGTGTAAAGCAATGAACCGTTCTTTCATCAGCGTAAT
TGCTGGTGGCTTCGGTCAAGACGTTGTTGCTTCATCTGGTGATGAAGAGCAGGGTGAGCATCGTGAAACAACCGCTGAAG
AAGTAGCAGAAATGCTTAAAAACTCTAAGTCTGTTGTTATCACTCCAGGATACGGCATGGCAGTAGCTCAAGCTCAGTAC
CCAGTTCACGAAATCACTGAAAAACTTCGTGCTCAAGGTGTTGAAGTGCGCTTTGGTATCCACCCAGTTGCGGGACGTTT
ACCTGGTCACATGAACGTTCTTCTTGCAGAAGCTAAAGTACCTTACGATATCGTTCTAGAAATGGACGAGATCAACGATG
ACTTATCTGAAACAGATACAGTTCTAGTTATTGGTGCTAACGATACAGTTAACCCTGCAGCAATGGAAGATCCAAACAGC
CCAATCGCAGGTATGCCAGTTCTAGAAGTATGGAATGCGAAAAACGTAATCGTATTCAAACGTTCTATGAACACAGGTTA
TGCTGGTGTTCAAAACCCACTGTTCTTTAAAGAAAATACTGAAATGCTTTTCGGTGACGCTAAAGAAAGTTGTGTGGAAA
TCAGCAAACATATCTAA

Upstream 100 bases:

>100_bases
TTATCGCAGTTCTTATCGCAAGTATTAATATATTTGGCGGTTTCGCTGTAACCAAGCGTATGCTTGAAATGTTCCGTAAA
GATAAATAAGGAGTAGGACA

Downstream 100 bases:

>100_bases
TATGCTAGTTTAATAGAGTCGATAAGCCGAGATGTAATGTCTCGGCTTTTTTGTATCTGTAATATATCAATGAGCATAAA
AATTCAGTTGGTTAGAAAAA

Product: pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Pyridine nucleotide transhydrogenase subunit beta [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSAGLVQAAYIVSAVLFIMSLAGLSKQETARSGNYYGITGMAIALIATIFGPDSTGTGWIIVAMAMGGAIGLYYAKKVEM
TEMPELVAILHSFVGMAAVLVGFNSYIDHGVLVGAMLNIHLVEVFLGVFIGAVTFTGSLVAFGKLRGVINSSALQLPHRH
KLNLLALVVSAGLLYHFVSVEGSMFALILVTLIAFAFGYHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVT
GALVGSSGAILSYIMCKAMNRSFISVIAGGFGQDVVASSGDEEQGEHRETTAEEVAEMLKNSKSVVITPGYGMAVAQAQY
PVHEITEKLRAQGVEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDLSETDTVLVIGANDTVNPAAMEDPNS
PIAGMPVLEVWNAKNVIVFKRSMNTGYAGVQNPLFFKENTEMLFGDAKESCVEISKHI

Sequences:

>Translated_458_residues
MSAGLVQAAYIVSAVLFIMSLAGLSKQETARSGNYYGITGMAIALIATIFGPDSTGTGWIIVAMAMGGAIGLYYAKKVEM
TEMPELVAILHSFVGMAAVLVGFNSYIDHGVLVGAMLNIHLVEVFLGVFIGAVTFTGSLVAFGKLRGVINSSALQLPHRH
KLNLLALVVSAGLLYHFVSVEGSMFALILVTLIAFAFGYHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVT
GALVGSSGAILSYIMCKAMNRSFISVIAGGFGQDVVASSGDEEQGEHRETTAEEVAEMLKNSKSVVITPGYGMAVAQAQY
PVHEITEKLRAQGVEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDLSETDTVLVIGANDTVNPAAMEDPNS
PIAGMPVLEVWNAKNVIVFKRSMNTGYAGVQNPLFFKENTEMLFGDAKESCVEISKHI
>Mature_457_residues
SAGLVQAAYIVSAVLFIMSLAGLSKQETARSGNYYGITGMAIALIATIFGPDSTGTGWIIVAMAMGGAIGLYYAKKVEMT
EMPELVAILHSFVGMAAVLVGFNSYIDHGVLVGAMLNIHLVEVFLGVFIGAVTFTGSLVAFGKLRGVINSSALQLPHRHK
LNLLALVVSAGLLYHFVSVEGSMFALILVTLIAFAFGYHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTG
ALVGSSGAILSYIMCKAMNRSFISVIAGGFGQDVVASSGDEEQGEHRETTAEEVAEMLKNSKSVVITPGYGMAVAQAQYP
VHEITEKLRAQGVEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDLSETDTVLVIGANDTVNPAAMEDPNSP
IAGMPVLEVWNAKNVIVFKRSMNTGYAGVQNPLFFKENTEMLFGDAKESCVEISKHI

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=454, Percent_Identity=52.6431718061674, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI122939153, Length=454, Percent_Identity=52.6431718061674, Blast_Score=452, Evalue=1e-127,
Organism=Escherichia coli, GI1787886, Length=460, Percent_Identity=78.695652173913, Blast_Score=718, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=453, Percent_Identity=51.6556291390728, Blast_Score=422, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 48494; Mature: 48363

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGLVQAAYIVSAVLFIMSLAGLSKQETARSGNYYGITGMAIALIATIFGPDSTGTGWI
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
IVAMAMGGAIGLYYAKKVEMTEMPELVAILHSFVGMAAVLVGFNSYIDHGVLVGAMLNIH
EEEEHHCCHHEEHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEVFLGVFIGAVTFTGSLVAFGKLRGVINSSALQLPHRHKLNLLALVVSAGLLYHFVSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
EGSMFALILVTLIAFAFGYHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEE
GALVGSSGAILSYIMCKAMNRSFISVIAGGFGQDVVASSGDEEQGEHRETTAEEVAEMLK
EEHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
NSKSVVITPGYGMAVAQAQYPVHEITEKLRAQGVEVRFGIHPVAGRLPGHMNVLLAEAKV
CCCCEEEECCCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCHHCCCCCCCCEEEEECCCC
PYDIVLEMDEINDDLSETDTVLVIGANDTVNPAAMEDPNSPIAGMPVLEVWNAKNVIVFK
CEEEEEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHEECCCCCEEEEE
RSMNTGYAGVQNPLFFKENTEMLFGDAKESCVEISKHI
ECCCCCCCCCCCCEEEECCCCEEECCHHHHHHHHHHCC
>Mature Secondary Structure 
SAGLVQAAYIVSAVLFIMSLAGLSKQETARSGNYYGITGMAIALIATIFGPDSTGTGWI
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
IVAMAMGGAIGLYYAKKVEMTEMPELVAILHSFVGMAAVLVGFNSYIDHGVLVGAMLNIH
EEEEHHCCHHEEHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEVFLGVFIGAVTFTGSLVAFGKLRGVINSSALQLPHRHKLNLLALVVSAGLLYHFVSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
EGSMFALILVTLIAFAFGYHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEE
GALVGSSGAILSYIMCKAMNRSFISVIAGGFGQDVVASSGDEEQGEHRETTAEEVAEMLK
EEHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
NSKSVVITPGYGMAVAQAQYPVHEITEKLRAQGVEVRFGIHPVAGRLPGHMNVLLAEAKV
CCCCEEEECCCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCHHCCCCCCCCEEEEECCCC
PYDIVLEMDEINDDLSETDTVLVIGANDTVNPAAMEDPNSPIAGMPVLEVWNAKNVIVFK
CEEEEEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHEECCCCCEEEEE
RSMNTGYAGVQNPLFFKENTEMLFGDAKESCVEISKHI
ECCCCCCCCCCCCEEEECCCCEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]