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The map label for this gene is hlyB [H]

Identifier: 59713630

GI number: 59713630

Start: 513408

End: 515036

Strand: Reverse

Name: hlyB [H]

Synonym: VF_A0447

Alternate gene names: 59713630

Gene position: 515036-513408 (Counterclockwise)

Preceding gene: 59713631

Following gene: 59713624

Centisome position: 38.71

GC content: 37.75

Gene sequence:

>1629_bases
ATGCAATTTTCACTTAAAAATACGTCTATTCGCGTTCAAGTTTTACTGCCAGTATTATTTACAGCATTGGCTTTGTTCTT
TTCTCTTTGGTATACATCAGTAAGCCTTGATAAAGAGCAAGATATTCTAAATAGTAATACGACTTCATTTATTTTCTACA
AAGATCAATTGGCAAAAATTGATGATCATATGTACCCAATGCGTATCAGCGCTGTTTATGCCATCTATGATCACGAACGT
AGAATCGATTTCTTAAAGAGTTTAAGCCAAAATGAGAAGATCCTAAACGAACTTCTTATTGAAATCTCTGCTCATAAAAC
CTTCAGCTCTGACGCTGCAATAGTTAAAAAAGCGGTTCATGACTATTTAGATTTCTCTAAAAAGATTCTTAACTTTCAAG
AACAAAAAGAGCAAGGAATAACAACTGGCGAAGATTACCATTCACTAATTGCTCAATACAGCAAGTTAGGGAATATTATG
GCGGAATCAATTAATACGCTATCTGCAAAAATTAATGATACCTCTACCAATGAAATAAACAAAAGCACAGAAAGAAACAC
GCAAGTTCAACACAATGCAATTATCACTGTTCTTATTGTTTTTGTTATTTCATTAGTCACATCTTGGTGGCTATCAGGCT
TGATCGTTCACCCAATCCAAAAACTTCAATTGGTTATCCGTAAATTAGCAGAAGGTAACTTAACGGTTCGTACTGATATT
GATGGCGAGAACGAAATTGCTCAATTAAGCCAAGACATCAACACCACTGCTATTCAACTTCAAGCAACCATTGAAGAGCT
TCACAACATCAACCAAAGCGTAGCTTCAGCATCGACTGAACTTGCAGCCGTAATGAATGAAGCTGAGCTTAACTCTCAAA
AAGAACTGTGTGAAATTGAACAAGTTGCTTCTGCTGTAAACGAGCTATCTAGTACCGCTAACAACGTAAGTGATAATGCA
TTAGCGGCAGATAAAACCGCTCAAAACACCAGTGATTTAGCTAAAGCTGGTTTAGATGTATTTACTCAAAGTACTGATGC
AAGTGAAAAAATGGCGGTTGCACTAACGGATGCTGCAGTTGTTGTAAATCGTTTAAAAGAGCAATCAGAGCAAATTAATA
ACGTTATTGAAGTGATCCGTAGTGTTTCAGAGCAGACTAATCTACTTGCTTTGAACGCAGCAATCGAAGCGGCACGTGCT
GGGGAATCTGGTCGTGGTTTTGCTGTGGTAGCAGATGAAGTTCGTTTACTTGCTGCACGTACGCAATCTTCAACTCAAGA
GATCCAAACCATTATTGAAGCCCTTCAAGAACAATCTGGTTTGGCTAATGAAAGTATGCAAACTAGCCTTGATATGCTGG
AGTTAAATAAAGAGCTAACGGCAAAAGCAGGCGATGCATTAATTGGTATTACTGAATCAATCAATGCCATTAACGACATG
AACACTCAAGTTGCTACGGCTGCAGAAGAACAATCGCAAGTAACTCAAGACATTAACCGTAACGTCGTTAATATGTCTGA
ATTAGTGAACCAAAACGTCACAGGCATCAGCCAAAGTGCCATTGCAAGTTCAGAACTGTCTCGATTAGCTGAAGAGCAGA
AACAACAACTGTCATTCTTTAAATTATAG

Upstream 100 bases:

>100_bases
CAATTTCAATCGACAAGAATGTGACAAAGGTCTATATTTCGAGAAAATTCTAACAATAAGACATATCTAACGATATTTTT
TCGAATTACAGAGGTTGAAA

Downstream 100 bases:

>100_bases
TTGTTCTCTTAATGAAAGAAAGCGCTAAGGCCACCCTCCTTAGCGCTTTTTCATATCATCTAGTTGGTTACATTGGTATT
ACAATGTAATTTTCTTTACC

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MQFSLKNTSIRVQVLLPVLFTALALFFSLWYTSVSLDKEQDILNSNTTSFIFYKDQLAKIDDHMYPMRISAVYAIYDHER
RIDFLKSLSQNEKILNELLIEISAHKTFSSDAAIVKKAVHDYLDFSKKILNFQEQKEQGITTGEDYHSLIAQYSKLGNIM
AESINTLSAKINDTSTNEINKSTERNTQVQHNAIITVLIVFVISLVTSWWLSGLIVHPIQKLQLVIRKLAEGNLTVRTDI
DGENEIAQLSQDINTTAIQLQATIEELHNINQSVASASTELAAVMNEAELNSQKELCEIEQVASAVNELSSTANNVSDNA
LAADKTAQNTSDLAKAGLDVFTQSTDASEKMAVALTDAAVVVNRLKEQSEQINNVIEVIRSVSEQTNLLALNAAIEAARA
GESGRGFAVVADEVRLLAARTQSSTQEIQTIIEALQEQSGLANESMQTSLDMLELNKELTAKAGDALIGITESINAINDM
NTQVATAAEEQSQVTQDINRNVVNMSELVNQNVTGISQSAIASSELSRLAEEQKQQLSFFKL

Sequences:

>Translated_542_residues
MQFSLKNTSIRVQVLLPVLFTALALFFSLWYTSVSLDKEQDILNSNTTSFIFYKDQLAKIDDHMYPMRISAVYAIYDHER
RIDFLKSLSQNEKILNELLIEISAHKTFSSDAAIVKKAVHDYLDFSKKILNFQEQKEQGITTGEDYHSLIAQYSKLGNIM
AESINTLSAKINDTSTNEINKSTERNTQVQHNAIITVLIVFVISLVTSWWLSGLIVHPIQKLQLVIRKLAEGNLTVRTDI
DGENEIAQLSQDINTTAIQLQATIEELHNINQSVASASTELAAVMNEAELNSQKELCEIEQVASAVNELSSTANNVSDNA
LAADKTAQNTSDLAKAGLDVFTQSTDASEKMAVALTDAAVVVNRLKEQSEQINNVIEVIRSVSEQTNLLALNAAIEAARA
GESGRGFAVVADEVRLLAARTQSSTQEIQTIIEALQEQSGLANESMQTSLDMLELNKELTAKAGDALIGITESINAINDM
NTQVATAAEEQSQVTQDINRNVVNMSELVNQNVTGISQSAIASSELSRLAEEQKQQLSFFKL
>Mature_542_residues
MQFSLKNTSIRVQVLLPVLFTALALFFSLWYTSVSLDKEQDILNSNTTSFIFYKDQLAKIDDHMYPMRISAVYAIYDHER
RIDFLKSLSQNEKILNELLIEISAHKTFSSDAAIVKKAVHDYLDFSKKILNFQEQKEQGITTGEDYHSLIAQYSKLGNIM
AESINTLSAKINDTSTNEINKSTERNTQVQHNAIITVLIVFVISLVTSWWLSGLIVHPIQKLQLVIRKLAEGNLTVRTDI
DGENEIAQLSQDINTTAIQLQATIEELHNINQSVASASTELAAVMNEAELNSQKELCEIEQVASAVNELSSTANNVSDNA
LAADKTAQNTSDLAKAGLDVFTQSTDASEKMAVALTDAAVVVNRLKEQSEQINNVIEVIRSVSEQTNLLALNAAIEAARA
GESGRGFAVVADEVRLLAARTQSSTQEIQTIIEALQEQSGLANESMQTSLDMLELNKELTAKAGDALIGITESINAINDM
NTQVATAAEEQSQVTQDINRNVVNMSELVNQNVTGISQSAIASSELSRLAEEQKQQLSFFKL

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788194, Length=361, Percent_Identity=28.808864265928, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1787690, Length=376, Percent_Identity=29.2553191489362, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI2367378, Length=321, Percent_Identity=29.2834890965732, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1788195, Length=336, Percent_Identity=29.7619047619048, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1789453, Length=316, Percent_Identity=26.2658227848101, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 59634; Mature: 59634

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2 ; PS50192 T_SNARE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSLKNTSIRVQVLLPVLFTALALFFSLWYTSVSLDKEQDILNSNTTSFIFYKDQLAKI
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHH
DDHMYPMRISAVYAIYDHERRIDFLKSLSQNEKILNELLIEISAHKTFSSDAAIVKKAVH
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DYLDFSKKILNFQEQKEQGITTGEDYHSLIAQYSKLGNIMAESINTLSAKINDTSTNEIN
HHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
KSTERNTQVQHNAIITVLIVFVISLVTSWWLSGLIVHPIQKLQLVIRKLAEGNLTVRTDI
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
DGENEIAQLSQDINTTAIQLQATIEELHNINQSVASASTELAAVMNEAELNSQKELCEIE
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QVASAVNELSSTANNVSDNALAADKTAQNTSDLAKAGLDVFTQSTDASEKMAVALTDAAV
HHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHEEEHHHHHH
VVNRLKEQSEQINNVIEVIRSVSEQTNLLALNAAIEAARAGESGRGFAVVADEVRLLAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCCEEEEHHHHHHHHHH
TQSSTQEIQTIIEALQEQSGLANESMQTSLDMLELNKELTAKAGDALIGITESINAINDM
HCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHC
NTQVATAAEEQSQVTQDINRNVVNMSELVNQNVTGISQSAIASSELSRLAEEQKQQLSFF
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
KL
CC
>Mature Secondary Structure
MQFSLKNTSIRVQVLLPVLFTALALFFSLWYTSVSLDKEQDILNSNTTSFIFYKDQLAKI
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHH
DDHMYPMRISAVYAIYDHERRIDFLKSLSQNEKILNELLIEISAHKTFSSDAAIVKKAVH
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DYLDFSKKILNFQEQKEQGITTGEDYHSLIAQYSKLGNIMAESINTLSAKINDTSTNEIN
HHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
KSTERNTQVQHNAIITVLIVFVISLVTSWWLSGLIVHPIQKLQLVIRKLAEGNLTVRTDI
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
DGENEIAQLSQDINTTAIQLQATIEELHNINQSVASASTELAAVMNEAELNSQKELCEIE
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QVASAVNELSSTANNVSDNALAADKTAQNTSDLAKAGLDVFTQSTDASEKMAVALTDAAV
HHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHEEEHHHHHH
VVNRLKEQSEQINNVIEVIRSVSEQTNLLALNAAIEAARAGESGRGFAVVADEVRLLAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCCEEEEHHHHHHHHHH
TQSSTQEIQTIIEALQEQSGLANESMQTSLDMLELNKELTAKAGDALIGITESINAINDM
HCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHC
NTQVATAAEEQSQVTQDINRNVVNMSELVNQNVTGISQSAIASSELSRLAEEQKQQLSFF
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
KL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]