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The map label for this gene is focA [H]

Identifier: 59713569

GI number: 59713569

Start: 436886

End: 438349

Strand: Reverse

Name: focA [H]

Synonym: VF_A0386

Alternate gene names: 59713569

Gene position: 438349-436886 (Counterclockwise)

Preceding gene: 59713573

Following gene: 59713566

Centisome position: 32.95

GC content: 39.34

Gene sequence:

>1464_bases
ATGAGTTCTAATCTCAGTCCAAATACAACTGGACAATTTAATCCCAGCGAAATGATGGAACAAGCAGCTTATTACGCACT
TGCTAAGTCTAAGAAGCCAACAAGCATGGTAATTGGCTTAGCCATTATGGCTGGTGTCTTTATCGGGATAGCATTCATTT
TCTATACAACGGTAACGACAGGCAATACAGAAACAGGTTGGGGTATCAGTCGTTTTGCTGGTGGCTTAGCTTTTAGTTTA
GGTTTGATCCTAGTTGTTATCGGTGGCGGTGAATTATTTACAAGCTCGGTTTTATCAGCAATCGCTGTAGCAAATAGACA
AATCAGTTTTGGCAAAATGCTAGGGATCTGGGGAAAAGTATATATCGGTAACTTTATTGGTGCTGGTTTATTACTCGCTC
TTGTTGTTTCAGCTGGTATGTACCAAAACGATGGTGGTCAATGGGGTTTGAACGCGCTAAATATCGCTCAACATAAATTG
CATCACCAACCGTTAGAAGCATTTGCTCTGGGTATTTTATGTAATTTATTAGTGTGTTTGGCAGTGTGGTTAACCTTCTG
CTCAACCAATATGCTCACTAAAGCAGTAATGGTTATTTTACCTGTTGCAATGTTTGTTAGTAGTGGCTTTGAGCACTGCG
TTGCAAACATGTTTATGGTTCCTTTAGGTATTGCGATTCAACAACTTGCTCCGCCTGAGTTTTGGCTTGCAGTCAGTGCA
ACACCTGAACAATACGCAGATTTAAATATCGTTAATTTTATTACAGCAAATTTAATCCCTGTAACGTTAGGAAATATCGT
TGGTGGTGCTGGTCTTGTTGGTTTGGCTTACTGGTCAATCTTCACTCGCCCTCAATTAAAAGCAGTAGAAACATCACAAA
TTACACCACTATTTACAGATTCAGACTCTAAGGAAATGATCAAAATGAACGCGAACAAAACAGTAAATCAATTTATGGAT
GTTAACACTTACGTTCTTCAACCAGAAATGCCTGTAGAAGTTGCACTGGATAACCTATTAGACCGCCATCTTTCAGGTGC
AATCGTTGTAAACAGTCATAGTGAAGTGGTTGGTTTCTTCTCTGAGCATGATGTATTAGTTGAACTATGGTGTGAAGATT
ATCTACCAGAAAAAGGTCGTACAGTTGCTGATTTAATGAAAACAGACATTACAAGTATCTCACCTAAAGACACATTATTG
CAGTTAGCTGAGTTCTACGCGATTGATAAACCAAAGCTTTACCCAACAACAGACATGGGCATTGCAACAAGCTACAGCAC
ACTATCAATTAATGACCGCGCTCGTGACATGCGTGTATCTAAACCACGTATCTTACCTGTAATTGAAAACAATCAATTAG
TTGGCGTTGTTACTCGTGAAGATGTTGTTAGCCAATTACGCTCTATTTACGGTGAACGTATGGAAGCAGTAAAAGCTGAA
GAAGTAGATGCTATCGTAGCTTAA

Upstream 100 bases:

>100_bases
TTACCCCAAGCACTTTTGCTCATAATCTAACTGGGCTTAAGCAAGACGGGTTAGCTCATATTCCTTAAGCATTTTTATAA
CCAATACTTAAGGAGGACAA

Downstream 100 bases:

>100_bases
AAAAATAACAACGTACGGTTGGCAAATAAAAGGGCTTCTAATATTAGAAGCCCTTTTCTATTAATTATTACGATTCTTTA
TATATGTATCTTGAAGAATA

Product: formate/nitrite transporter family protein

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSSNLSPNTTGQFNPSEMMEQAAYYALAKSKKPTSMVIGLAIMAGVFIGIAFIFYTTVTTGNTETGWGISRFAGGLAFSL
GLILVVIGGGELFTSSVLSAIAVANRQISFGKMLGIWGKVYIGNFIGAGLLLALVVSAGMYQNDGGQWGLNALNIAQHKL
HHQPLEAFALGILCNLLVCLAVWLTFCSTNMLTKAVMVILPVAMFVSSGFEHCVANMFMVPLGIAIQQLAPPEFWLAVSA
TPEQYADLNIVNFITANLIPVTLGNIVGGAGLVGLAYWSIFTRPQLKAVETSQITPLFTDSDSKEMIKMNANKTVNQFMD
VNTYVLQPEMPVEVALDNLLDRHLSGAIVVNSHSEVVGFFSEHDVLVELWCEDYLPEKGRTVADLMKTDITSISPKDTLL
QLAEFYAIDKPKLYPTTDMGIATSYSTLSINDRARDMRVSKPRILPVIENNQLVGVVTREDVVSQLRSIYGERMEAVKAE
EVDAIVA

Sequences:

>Translated_487_residues
MSSNLSPNTTGQFNPSEMMEQAAYYALAKSKKPTSMVIGLAIMAGVFIGIAFIFYTTVTTGNTETGWGISRFAGGLAFSL
GLILVVIGGGELFTSSVLSAIAVANRQISFGKMLGIWGKVYIGNFIGAGLLLALVVSAGMYQNDGGQWGLNALNIAQHKL
HHQPLEAFALGILCNLLVCLAVWLTFCSTNMLTKAVMVILPVAMFVSSGFEHCVANMFMVPLGIAIQQLAPPEFWLAVSA
TPEQYADLNIVNFITANLIPVTLGNIVGGAGLVGLAYWSIFTRPQLKAVETSQITPLFTDSDSKEMIKMNANKTVNQFMD
VNTYVLQPEMPVEVALDNLLDRHLSGAIVVNSHSEVVGFFSEHDVLVELWCEDYLPEKGRTVADLMKTDITSISPKDTLL
QLAEFYAIDKPKLYPTTDMGIATSYSTLSINDRARDMRVSKPRILPVIENNQLVGVVTREDVVSQLRSIYGERMEAVKAE
EVDAIVA
>Mature_486_residues
SSNLSPNTTGQFNPSEMMEQAAYYALAKSKKPTSMVIGLAIMAGVFIGIAFIFYTTVTTGNTETGWGISRFAGGLAFSLG
LILVVIGGGELFTSSVLSAIAVANRQISFGKMLGIWGKVYIGNFIGAGLLLALVVSAGMYQNDGGQWGLNALNIAQHKLH
HQPLEAFALGILCNLLVCLAVWLTFCSTNMLTKAVMVILPVAMFVSSGFEHCVANMFMVPLGIAIQQLAPPEFWLAVSAT
PEQYADLNIVNFITANLIPVTLGNIVGGAGLVGLAYWSIFTRPQLKAVETSQITPLFTDSDSKEMIKMNANKTVNQFMDV
NTYVLQPEMPVEVALDNLLDRHLSGAIVVNSHSEVVGFFSEHDVLVELWCEDYLPEKGRTVADLMKTDITSISPKDTLLQ
LAEFYAIDKPKLYPTTDMGIATSYSTLSINDRARDMRVSKPRILPVIENNQLVGVVTREDVVSQLRSIYGERMEAVKAEE
VDAIVA

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=269, Percent_Identity=50.9293680297398, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788837, Length=285, Percent_Identity=38.5964912280702, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI87082249, Length=246, Percent_Identity=29.6747967479675, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321779, Length=203, Percent_Identity=26.1083743842365, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 52870; Mature: 52739

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNLSPNTTGQFNPSEMMEQAAYYALAKSKKPTSMVIGLAIMAGVFIGIAFIFYTTVTT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
GNTETGWGISRFAGGLAFSLGLILVVIGGGELFTSSVLSAIAVANRQISFGKMLGIWGKV
CCCCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YIGNFIGAGLLLALVVSAGMYQNDGGQWGLNALNIAQHKLHHQPLEAFALGILCNLLVCL
HHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AVWLTFCSTNMLTKAVMVILPVAMFVSSGFEHCVANMFMVPLGIAIQQLAPPEFWLAVSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
TPEQYADLNIVNFITANLIPVTLGNIVGGAGLVGLAYWSIFTRPQLKAVETSQITPLFTD
CHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEECCCCCCEEEEC
SDSKEMIKMNANKTVNQFMDVNTYVLQPEMPVEVALDNLLDRHLSGAIVVNSHSEVVGFF
CCCHHHEEECCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCHHEEEC
SEHDVLVELWCEDYLPEKGRTVADLMKTDITSISPKDTLLQLAEFYAIDKPKLYPTTDMG
CCCCHHHHEEHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
IATSYSTLSINDRARDMRVSKPRILPVIENNQLVGVVTREDVVSQLRSIYGERMEAVKAE
CEECCEEEEECCCHHCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
EVDAIVA
HHHHHCC
>Mature Secondary Structure 
SSNLSPNTTGQFNPSEMMEQAAYYALAKSKKPTSMVIGLAIMAGVFIGIAFIFYTTVTT
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
GNTETGWGISRFAGGLAFSLGLILVVIGGGELFTSSVLSAIAVANRQISFGKMLGIWGKV
CCCCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YIGNFIGAGLLLALVVSAGMYQNDGGQWGLNALNIAQHKLHHQPLEAFALGILCNLLVCL
HHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AVWLTFCSTNMLTKAVMVILPVAMFVSSGFEHCVANMFMVPLGIAIQQLAPPEFWLAVSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
TPEQYADLNIVNFITANLIPVTLGNIVGGAGLVGLAYWSIFTRPQLKAVETSQITPLFTD
CHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEECCCCCCEEEEC
SDSKEMIKMNANKTVNQFMDVNTYVLQPEMPVEVALDNLLDRHLSGAIVVNSHSEVVGFF
CCCHHHEEECCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCHHEEEC
SEHDVLVELWCEDYLPEKGRTVADLMKTDITSISPKDTLLQLAEFYAIDKPKLYPTTDMG
CCCCHHHHEEHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
IATSYSTLSINDRARDMRVSKPRILPVIENNQLVGVVTREDVVSQLRSIYGERMEAVKAE
CEECCEEEEECCCHHCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
EVDAIVA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]