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The map label for this gene is mltF [H]

Identifier: 59713468

GI number: 59713468

Start: 316802

End: 318220

Strand: Reverse

Name: mltF [H]

Synonym: VF_A0285

Alternate gene names: 59713468

Gene position: 318220-316802 (Counterclockwise)

Preceding gene: 59713469

Following gene: 59713463

Centisome position: 23.92

GC content: 36.5

Gene sequence:

>1419_bases
ATGGATCGTTTACGTCGCGTTTTCTGGGTATTATTACTACTTCCTCAGCTCGCTTTTGCAATAGAGCTTTCCCCGTTAAC
TCAAACACCTTATGTTGGCGATTTGAAGGAACTAGAGAAAAAAGGAACGATACGAGTATTAGTATCTGCTGATCTTGGCT
TTTATTACATAGAAAAAGGGCAACCGAAGGGGATCATTGCCGAATTTCTTCATCATTTCGAATTACATTTACGCAAAAAT
AAAATTCACGTTAATGTTCAAGTCATCCCTATCCCTCGGGATGATTTATTTACCGCTTTAGAACGAGGCTTTGGTGATCT
TGCCGTTGCCAACCTAACGATTACCCCGCAGCGACTACAATATGTCGATTTTAGTGATCCTGTGATCAGTAACAGCCAAG
AGTGGCTCGTTAGCTCTCCATCTACACCTCCTTTAGAATCTCTAGAAGATTTATCAGGTAAAGAAGTATGGGTTAGACCA
AGCTCAAGCTATTTTGAGAGCTTACAGCGAATTAATAAGCAGTTATCTTCCCAAGGGATCTCCCCTATCAATGTTCAATT
CTTAGAAGAAAACTTACAAGACTATGAATTAATGGAAATGCTCAACCAAGATATTTTACCAATTACGGTTATCGATAGTC
ATAAATCTCGTCTTTGGACTCAAGTCATGAAGAATATCACCATATATGAAACTCTTCCGATTAGAGAAAACGCCAATATT
GGTTGGGCAATGAGAAAAAACAGCCCAGAATTAAAAACAGCAGTAAACAAATACATTAGAACTATTCGACAAGGATCTTT
TTTAGGTAATGTTATTTATAAAAAATACCTTGCTAACACCAAGTGGCTAAAAAAAGCAGTAGACCCTGAAACAGTAAAAC
AATTTAAAAGTTTAGCGACGCTCTTTGAGCAATATGCGGATAAATATGAATTTGATTGGTTAATGATTTCAGCTCAAGCT
TATCAAGAGTCCAAATTCAATAACCGTCTTGTCTCACATATGGGTGCTGTTGGAATCATGCAAGTTTTACCGAAGACGGC
AAGAGAACCTTATATCAATATCAAAAACTTCAGAGAACTAGAAAATAACATCCATGCTGGTGTTAAATATATGAATTTTG
TACATAAACGTTACTTTTTAAAACCAGAGATAACAGAAGAGAACCAAATGTACTTTTCTTTAGCCGCTTATAATGCTGGC
CCTGCTAACGTTCGAAAAATGCGTAGAATGGCAGTAAAACACGGTTATAACCCTAATATTTGGTTTAATAACGTCGAGAT
AATGGCAAGGAAGTACGTAAGTAAAGAGCCTGTTCACTATGTTGCAAATATCAGTCGCTATTACGTTATTTATAAACAAA
TAGCTCAACTGCAAACACACAGAGAACAGAGAAACGCCTCAAATTATAAGTTATTTTAA

Upstream 100 bases:

>100_bases
TAATATCTAATGTAAATCGAGAGCATAGAATGAAGAGGACTTGAATTTCATCTAATTTCAACATAAATTGTTAATACACT
GTTAAACAAGGACTCATAAC

Downstream 100 bases:

>100_bases
TTTCACTGTGACTAAACAAAAAAACGCCCAATCTATATTGGGCGTTTTTATTCATAAACCTACAATAAAAAATGCGTTAT
TTTTCAGCTTTTACTTTTGG

Product: amino-acid ABC transporter binding protein

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MDRLRRVFWVLLLLPQLAFAIELSPLTQTPYVGDLKELEKKGTIRVLVSADLGFYYIEKGQPKGIIAEFLHHFELHLRKN
KIHVNVQVIPIPRDDLFTALERGFGDLAVANLTITPQRLQYVDFSDPVISNSQEWLVSSPSTPPLESLEDLSGKEVWVRP
SSSYFESLQRINKQLSSQGISPINVQFLEENLQDYELMEMLNQDILPITVIDSHKSRLWTQVMKNITIYETLPIRENANI
GWAMRKNSPELKTAVNKYIRTIRQGSFLGNVIYKKYLANTKWLKKAVDPETVKQFKSLATLFEQYADKYEFDWLMISAQA
YQESKFNNRLVSHMGAVGIMQVLPKTAREPYINIKNFRELENNIHAGVKYMNFVHKRYFLKPEITEENQMYFSLAAYNAG
PANVRKMRRMAVKHGYNPNIWFNNVEIMARKYVSKEPVHYVANISRYYVIYKQIAQLQTHREQRNASNYKLF

Sequences:

>Translated_472_residues
MDRLRRVFWVLLLLPQLAFAIELSPLTQTPYVGDLKELEKKGTIRVLVSADLGFYYIEKGQPKGIIAEFLHHFELHLRKN
KIHVNVQVIPIPRDDLFTALERGFGDLAVANLTITPQRLQYVDFSDPVISNSQEWLVSSPSTPPLESLEDLSGKEVWVRP
SSSYFESLQRINKQLSSQGISPINVQFLEENLQDYELMEMLNQDILPITVIDSHKSRLWTQVMKNITIYETLPIRENANI
GWAMRKNSPELKTAVNKYIRTIRQGSFLGNVIYKKYLANTKWLKKAVDPETVKQFKSLATLFEQYADKYEFDWLMISAQA
YQESKFNNRLVSHMGAVGIMQVLPKTAREPYINIKNFRELENNIHAGVKYMNFVHKRYFLKPEITEENQMYFSLAAYNAG
PANVRKMRRMAVKHGYNPNIWFNNVEIMARKYVSKEPVHYVANISRYYVIYKQIAQLQTHREQRNASNYKLF
>Mature_472_residues
MDRLRRVFWVLLLLPQLAFAIELSPLTQTPYVGDLKELEKKGTIRVLVSADLGFYYIEKGQPKGIIAEFLHHFELHLRKN
KIHVNVQVIPIPRDDLFTALERGFGDLAVANLTITPQRLQYVDFSDPVISNSQEWLVSSPSTPPLESLEDLSGKEVWVRP
SSSYFESLQRINKQLSSQGISPINVQFLEENLQDYELMEMLNQDILPITVIDSHKSRLWTQVMKNITIYETLPIRENANI
GWAMRKNSPELKTAVNKYIRTIRQGSFLGNVIYKKYLANTKWLKKAVDPETVKQFKSLATLFEQYADKYEFDWLMISAQA
YQESKFNNRLVSHMGAVGIMQVLPKTAREPYINIKNFRELENNIHAGVKYMNFVHKRYFLKPEITEENQMYFSLAAYNAG
PANVRKMRRMAVKHGYNPNIWFNNVEIMARKYVSKEPVHYVANISRYYVIYKQIAQLQTHREQRNASNYKLF

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=443, Percent_Identity=25.2821670428894, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 55101; Mature: 55101

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLRRVFWVLLLLPQLAFAIELSPLTQTPYVGDLKELEKKGTIRVLVSADLGFYYIEKG
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCCCCEEEEEEECCCEEEEECC
QPKGIIAEFLHHFELHLRKNKIHVNVQVIPIPRDDLFTALERGFGDLAVANLTITPQRLQ
CCCCHHHHHHHHHHHHEEECEEEEEEEEEECCCHHHHHHHHCCCCCEEEEEEEECHHHHE
YVDFSDPVISNSQEWLVSSPSTPPLESLEDLSGKEVWVRPSSSYFESLQRINKQLSSQGI
EECCCCCCCCCCCCEEEECCCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCC
SPINVQFLEENLQDYELMEMLNQDILPITVIDSHKSRLWTQVMKNITIYETLPIRENANI
CCCCHHHHHHCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCCC
GWAMRKNSPELKTAVNKYIRTIRQGSFLGNVIYKKYLANTKWLKKAVDPETVKQFKSLAT
CEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHH
LFEQYADKYEFDWLMISAQAYQESKFNNRLVSHMGAVGIMQVLPKTAREPYINIKNFREL
HHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ENNIHAGVKYMNFVHKRYFLKPEITEENQMYFSLAAYNAGPANVRKMRRMAVKHGYNPNI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCE
WFNNVEIMARKYVSKEPVHYVANISRYYVIYKQIAQLQTHREQRNASNYKLF
EECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDRLRRVFWVLLLLPQLAFAIELSPLTQTPYVGDLKELEKKGTIRVLVSADLGFYYIEKG
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCCCCEEEEEEECCCEEEEECC
QPKGIIAEFLHHFELHLRKNKIHVNVQVIPIPRDDLFTALERGFGDLAVANLTITPQRLQ
CCCCHHHHHHHHHHHHEEECEEEEEEEEEECCCHHHHHHHHCCCCCEEEEEEEECHHHHE
YVDFSDPVISNSQEWLVSSPSTPPLESLEDLSGKEVWVRPSSSYFESLQRINKQLSSQGI
EECCCCCCCCCCCCEEEECCCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCC
SPINVQFLEENLQDYELMEMLNQDILPITVIDSHKSRLWTQVMKNITIYETLPIRENANI
CCCCHHHHHHCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCCC
GWAMRKNSPELKTAVNKYIRTIRQGSFLGNVIYKKYLANTKWLKKAVDPETVKQFKSLAT
CEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHH
LFEQYADKYEFDWLMISAQAYQESKFNNRLVSHMGAVGIMQVLPKTAREPYINIKNFREL
HHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ENNIHAGVKYMNFVHKRYFLKPEITEENQMYFSLAAYNAGPANVRKMRRMAVKHGYNPNI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCE
WFNNVEIMARKYVSKEPVHYVANISRYYVIYKQIAQLQTHREQRNASNYKLF
EECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA