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The map label for this gene is mltF [H]

Identifier: 59713469

GI number: 59713469

Start: 318311

End: 319744

Strand: Reverse

Name: mltF [H]

Synonym: VF_A0286

Alternate gene names: 59713469

Gene position: 319744-318311 (Counterclockwise)

Preceding gene: 59713470

Following gene: 59713468

Centisome position: 24.03

GC content: 33.89

Gene sequence:

>1434_bases
ATGAATCTATTCCATAAGTTATTTTTTACTCTACTTATATTACCTATAAACGCTGAAGCACTTGAGCTAGCCCCTCTAAG
TAACAAATCCTATAAGGGTGATTACAATGAAATATCAAAAAAAGGGGTAATTCGTGTTCTGGTTTCAATGGAAGTTGGTT
TTTATCAAGTAAGTAATGGTAAACCCATCGGAATAATTTCAGAATTTCTCTCACACTTTGAGCGTCATTTAGCGAAAAAA
AATGATCACACTCATATTCGAATTATTCCCGTCTCTGATGATGATTTAATATGGTCATTAAAAGCAGGATTTGGAGACAT
TGCCGTTGGCCACTTAGCGATCACCAAAGCTCGATTACGCCATATCGACTTTAGTACTCCCACAATAAAAGACAGTGAAG
AGTGGTTAATCACAGCTAAAGGACACGCCCCTATCACTGAGCTAGCCGATCTTTCAGGTAAAGAAATATGGGTAAAAGGC
AGCTCCAGTTACTTTGAGACACTTCAAAACATTAATGAACAACTGACGTCAAATAATATGGCTCCTATGGATGTTCATTT
TTTAGAAGAATCATTAGGTGATAATGAAGTTCTTGAGATGGTTGAAAATAAGTTATTACCAGCAACCGTAATAGAAAATT
ATCGAGCAATACTATGGAAAAAGATCATCCCTAACATTCAATATCATGAAGAATTCCCATTAAAACAAGATATTAATATC
GCATGGGCAGTACGAAAAAATAGCCCTCAGCTAACTAATGTCGTAAATCAATACATTACTAAAATAAAAAAAGGGTCATT
TCTAGGTAATTTAATTTATAAAAAATACCTTAATGACGAAAAGTGGTTAGCGAAAATTCTACAAACCGATAATATCGATA
AGCTTTACGAGCTGTCTGATTTATTTAAACACTACTCTAGTATGTATAACTTAGATTGGCAGCTAACTTCAGCCTTAGCT
TATCAAGAATCTAGGTTGGATAATAAAGCCGTATCTCATAAAGGTGCTGTTGGAATCATGCAAGTCTTACCGAGCACAGC
AAAAGATAAGCATGTTCAAATAGACAATATTTATACCTTAGAAAACAACATACATGCAGGTATTAAATACTTAAGCTTTG
TGCATAAACGATATTTTGATAAACCAGAAATATCCGCAGATAATCAGCTCTACTTCTCATTAGCCGCTTATAATGCAGGA
CCTGCTAAAATAAGACGCATTCGAAAAAAAGCGCAAGAACAAGGTTATGACCCTAATGTTTGGTTCAATAATGTGGAATT
AATGGCGTTGAAATATATCAGTAAAGAACCGGTTCATTATGTATCAAACATAAGTCGTTACTATGTAATTTATAAACAAA
TAGTTCAGTTAAAAGAACAAAAAGAGCAACGTAATGCGTTAAATAAAGCCACATTCGACTTTGATAATATCTAA

Upstream 100 bases:

>100_bases
GTCTCAAAAATAAAACGACAGAAAAACAGTAAAATAACGCCTTGTCTTTAACAGTATGGAAACATAAGTTGTAATCATGT
TGTATCATGGATGACAAACT

Downstream 100 bases:

>100_bases
TGTAAATCGAGAGCATAGAATGAAGAGGACTTGAATTTCATCTAATTTCAACATAAATTGTTAATACACTGTTAAACAAG
GACTCATAACATGGATCGTT

Product: amino-acid ABC transporter binding protein

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MNLFHKLFFTLLILPINAEALELAPLSNKSYKGDYNEISKKGVIRVLVSMEVGFYQVSNGKPIGIISEFLSHFERHLAKK
NDHTHIRIIPVSDDDLIWSLKAGFGDIAVGHLAITKARLRHIDFSTPTIKDSEEWLITAKGHAPITELADLSGKEIWVKG
SSSYFETLQNINEQLTSNNMAPMDVHFLEESLGDNEVLEMVENKLLPATVIENYRAILWKKIIPNIQYHEEFPLKQDINI
AWAVRKNSPQLTNVVNQYITKIKKGSFLGNLIYKKYLNDEKWLAKILQTDNIDKLYELSDLFKHYSSMYNLDWQLTSALA
YQESRLDNKAVSHKGAVGIMQVLPSTAKDKHVQIDNIYTLENNIHAGIKYLSFVHKRYFDKPEISADNQLYFSLAAYNAG
PAKIRRIRKKAQEQGYDPNVWFNNVELMALKYISKEPVHYVSNISRYYVIYKQIVQLKEQKEQRNALNKATFDFDNI

Sequences:

>Translated_477_residues
MNLFHKLFFTLLILPINAEALELAPLSNKSYKGDYNEISKKGVIRVLVSMEVGFYQVSNGKPIGIISEFLSHFERHLAKK
NDHTHIRIIPVSDDDLIWSLKAGFGDIAVGHLAITKARLRHIDFSTPTIKDSEEWLITAKGHAPITELADLSGKEIWVKG
SSSYFETLQNINEQLTSNNMAPMDVHFLEESLGDNEVLEMVENKLLPATVIENYRAILWKKIIPNIQYHEEFPLKQDINI
AWAVRKNSPQLTNVVNQYITKIKKGSFLGNLIYKKYLNDEKWLAKILQTDNIDKLYELSDLFKHYSSMYNLDWQLTSALA
YQESRLDNKAVSHKGAVGIMQVLPSTAKDKHVQIDNIYTLENNIHAGIKYLSFVHKRYFDKPEISADNQLYFSLAAYNAG
PAKIRRIRKKAQEQGYDPNVWFNNVELMALKYISKEPVHYVSNISRYYVIYKQIVQLKEQKEQRNALNKATFDFDNI
>Mature_477_residues
MNLFHKLFFTLLILPINAEALELAPLSNKSYKGDYNEISKKGVIRVLVSMEVGFYQVSNGKPIGIISEFLSHFERHLAKK
NDHTHIRIIPVSDDDLIWSLKAGFGDIAVGHLAITKARLRHIDFSTPTIKDSEEWLITAKGHAPITELADLSGKEIWVKG
SSSYFETLQNINEQLTSNNMAPMDVHFLEESLGDNEVLEMVENKLLPATVIENYRAILWKKIIPNIQYHEEFPLKQDINI
AWAVRKNSPQLTNVVNQYITKIKKGSFLGNLIYKKYLNDEKWLAKILQTDNIDKLYELSDLFKHYSSMYNLDWQLTSALA
YQESRLDNKAVSHKGAVGIMQVLPSTAKDKHVQIDNIYTLENNIHAGIKYLSFVHKRYFDKPEISADNQLYFSLAAYNAG
PAKIRRIRKKAQEQGYDPNVWFNNVELMALKYISKEPVHYVSNISRYYVIYKQIVQLKEQKEQRNALNKATFDFDNI

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=462, Percent_Identity=24.2424242424242, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082441, Length=126, Percent_Identity=32.5396825396825, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 54989; Mature: 54989

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFHKLFFTLLILPINAEALELAPLSNKSYKGDYNEISKKGVIRVLVSMEVGFYQVSNG
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHEEEEEEECCCEEEECCC
KPIGIISEFLSHFERHLAKKNDHTHIRIIPVSDDDLIWSLKAGFGDIAVGHLAITKARLR
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHH
HIDFSTPTIKDSEEWLITAKGHAPITELADLSGKEIWVKGSSSYFETLQNINEQLTSNNM
HCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCC
APMDVHFLEESLGDNEVLEMVENKLLPATVIENYRAILWKKIIPNIQYHEEFPLKQDINI
CCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
AWAVRKNSPQLTNVVNQYITKIKKGSFLGNLIYKKYLNDEKWLAKILQTDNIDKLYELSD
EEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHH
LFKHYSSMYNLDWQLTSALAYQESRLDNKAVSHKGAVGIMQVLPSTAKDKHVQIDNIYTL
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCCCCCCCCEEEECEEEEE
ENNIHAGIKYLSFVHKRYFDKPEISADNQLYFSLAAYNAGPAKIRRIRKKAQEQGYDPNV
CCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
WFNNVELMALKYISKEPVHYVSNISRYYVIYKQIVQLKEQKEQRNALNKATFDFDNI
EECCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNLFHKLFFTLLILPINAEALELAPLSNKSYKGDYNEISKKGVIRVLVSMEVGFYQVSNG
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHEEEEEEECCCEEEECCC
KPIGIISEFLSHFERHLAKKNDHTHIRIIPVSDDDLIWSLKAGFGDIAVGHLAITKARLR
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHH
HIDFSTPTIKDSEEWLITAKGHAPITELADLSGKEIWVKGSSSYFETLQNINEQLTSNNM
HCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCC
APMDVHFLEESLGDNEVLEMVENKLLPATVIENYRAILWKKIIPNIQYHEEFPLKQDINI
CCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
AWAVRKNSPQLTNVVNQYITKIKKGSFLGNLIYKKYLNDEKWLAKILQTDNIDKLYELSD
EEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHH
LFKHYSSMYNLDWQLTSALAYQESRLDNKAVSHKGAVGIMQVLPSTAKDKHVQIDNIYTL
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCCCCCCCCEEEECEEEEE
ENNIHAGIKYLSFVHKRYFDKPEISADNQLYFSLAAYNAGPAKIRRIRKKAQEQGYDPNV
CCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
WFNNVELMALKYISKEPVHYVSNISRYYVIYKQIVQLKEQKEQRNALNKATFDFDNI
EECCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA