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The map label for this gene is cusS [H]

Identifier: 59713361

GI number: 59713361

Start: 200245

End: 201585

Strand: Reverse

Name: cusS [H]

Synonym: VF_A0178

Alternate gene names: 59713361

Gene position: 201585-200245 (Counterclockwise)

Preceding gene: 59713362

Following gene: 59713358

Centisome position: 15.15

GC content: 34.97

Gene sequence:

>1341_bases
ATGAAAAAACATTACTTATCAATGAAACTAAAACTGACATTAATGTTTTTCTCTACCTCTGTATTTGTCTTTATTGGCTT
AGGTTTTGCCATTCAATACCTAGTCCAATCCCATTTTTATGAAGAAGATCTCCGTTTAATTAACGCCAATCGGACTTCCA
TTTTCGTCTTATTAGAAAATGAAGGCATAACTAGCAGTGAAATTTTTGCTTCCTTAAAAAGTCGCGGTATTAACATTTGG
ATTGCCAATGATAATGGCACATTGACACAAAATACCAATGTAATTCCAAATGACAGCGCCTTTAAACAATCAAAAAAAAT
CAACGAATGGACTTATGCAGGAAGTACATTTAGAACACTCTACTACCCACTAGAAAACGAGAATAATTATACCGGACTGC
TTATTGGTATTAATATTGATCATCACATCGAGTTCAATAAAAGACTAAAAGGCATTTTAATATGGTCAATTAGTATCGCG
AGCCTACTCTCTTTCATTTGCAGCTTTATCATCGTAAATATGGGGTTTAAACCTCTGCAATATTTACAAGATCGCATCAA
ACAAGTTCAGCCAAATAAGCTAAGTATTCGATTGTCTGATGAACACTTACCCAACGAGTTGGTTGAACTTGCAAAAATAC
AAAACCAGATGCTTGATCGATTAGAACTTGGGTTTGAACGACTCAGTGATTTTTCATCTGATATCGCTCATGAATTAAAA
ACGCCACTCTCAAATATTATGACTCAGACTCATGTCACCTTATCGCAACCAAGAACACCTCATGAATATCAAGAGATCTT
ATCTTCAAACTTAGAAGAATTAGAACGCATTAACAAAACAATTAACGATACCTTGTATCTTGCTAAATCTGAAAATGACT
TATTACTAAAAGACAATATGCAATTAGATTTAGAGCAACTCTTCGCCCCTATTATTGAGTACTACAGCATTGTGTCTGAA
GAAACAGGAGTAACAATTCATCTATCAGGGAACGGATCTCTTTGGGGTGATAAATCCATGATACAACGCGTAGCAAATAA
TCTACTCTCTAACGCTATACGTCACTCTGCACCTGATAGCAATATTCAAATTAAGATTGAGGAAAACCACAGTAACATCG
TGTTATCTATCTCGAATATAGGAGATAAGATTCCTGATAAAGATTTACCTTTTATTTTTGAACGCTTTTATCGAGCAGAC
AAATCCAGACAACATTCAAGTAGCACAGGTGCAGGCTTAGGCTTAACTATTGTGCGTTCAATTGTGAATCTACATAACGG
CGATATTTCCGTTCAATCAGAAGGAAAGAAAACCACTTTTTTGCTTACCTTTCCTTTATAG

Upstream 100 bases:

>100_bases
GCGGTAAAACGACTTCGTTCTAAAATAGATAAACCCTACGACACCCCACTAATTCATACTGTTCGCGGTATGGGTTATAA
AATGGACGAGTGCTAGTTAT

Downstream 100 bases:

>100_bases
AACATAAGTCAATGTACCTATTTGTTTGATTCTAGTGCCAATAATTTTTCTTTAATATCAACGCCACCAGCATATCCAGT
GAGGCTACCATTTTTGCCAA

Product: sensory histidine kinase in two-component regulatory system with CusR, senses copper ions

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKKHYLSMKLKLTLMFFSTSVFVFIGLGFAIQYLVQSHFYEEDLRLINANRTSIFVLLENEGITSSEIFASLKSRGINIW
IANDNGTLTQNTNVIPNDSAFKQSKKINEWTYAGSTFRTLYYPLENENNYTGLLIGINIDHHIEFNKRLKGILIWSISIA
SLLSFICSFIIVNMGFKPLQYLQDRIKQVQPNKLSIRLSDEHLPNELVELAKIQNQMLDRLELGFERLSDFSSDIAHELK
TPLSNIMTQTHVTLSQPRTPHEYQEILSSNLEELERINKTINDTLYLAKSENDLLLKDNMQLDLEQLFAPIIEYYSIVSE
ETGVTIHLSGNGSLWGDKSMIQRVANNLLSNAIRHSAPDSNIQIKIEENHSNIVLSISNIGDKIPDKDLPFIFERFYRAD
KSRQHSSSTGAGLGLTIVRSIVNLHNGDISVQSEGKKTTFLLTFPL

Sequences:

>Translated_446_residues
MKKHYLSMKLKLTLMFFSTSVFVFIGLGFAIQYLVQSHFYEEDLRLINANRTSIFVLLENEGITSSEIFASLKSRGINIW
IANDNGTLTQNTNVIPNDSAFKQSKKINEWTYAGSTFRTLYYPLENENNYTGLLIGINIDHHIEFNKRLKGILIWSISIA
SLLSFICSFIIVNMGFKPLQYLQDRIKQVQPNKLSIRLSDEHLPNELVELAKIQNQMLDRLELGFERLSDFSSDIAHELK
TPLSNIMTQTHVTLSQPRTPHEYQEILSSNLEELERINKTINDTLYLAKSENDLLLKDNMQLDLEQLFAPIIEYYSIVSE
ETGVTIHLSGNGSLWGDKSMIQRVANNLLSNAIRHSAPDSNIQIKIEENHSNIVLSISNIGDKIPDKDLPFIFERFYRAD
KSRQHSSSTGAGLGLTIVRSIVNLHNGDISVQSEGKKTTFLLTFPL
>Mature_446_residues
MKKHYLSMKLKLTLMFFSTSVFVFIGLGFAIQYLVQSHFYEEDLRLINANRTSIFVLLENEGITSSEIFASLKSRGINIW
IANDNGTLTQNTNVIPNDSAFKQSKKINEWTYAGSTFRTLYYPLENENNYTGLLIGINIDHHIEFNKRLKGILIWSISIA
SLLSFICSFIIVNMGFKPLQYLQDRIKQVQPNKLSIRLSDEHLPNELVELAKIQNQMLDRLELGFERLSDFSSDIAHELK
TPLSNIMTQTHVTLSQPRTPHEYQEILSSNLEELERINKTINDTLYLAKSENDLLLKDNMQLDLEQLFAPIIEYYSIVSE
ETGVTIHLSGNGSLWGDKSMIQRVANNLLSNAIRHSAPDSNIQIKIEENHSNIVLSISNIGDKIPDKDLPFIFERFYRAD
KSRQHSSSTGAGLGLTIVRSIVNLHNGDISVQSEGKKTTFLLTFPL

Specific function: Component of the sil cation-efflux system that confers resistance to silver. Probable member of a two-component regulatory system silS/silR. May activate silR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=324, Percent_Identity=34.8765432098765, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1788279, Length=310, Percent_Identity=32.9032258064516, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788393, Length=260, Percent_Identity=28.8461538461538, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790346, Length=260, Percent_Identity=25, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=25.2293577981651, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=29.4117647058824, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790861, Length=232, Percent_Identity=27.1551724137931, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790551, Length=304, Percent_Identity=27.9605263157895, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=25, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789403, Length=282, Percent_Identity=24.822695035461, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082128, Length=308, Percent_Identity=23.3766233766234, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=25.5605381165919, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=25.4098360655738, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=25.6637168141593, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787894, Length=298, Percent_Identity=25.503355704698, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788549, Length=228, Percent_Identity=26.7543859649123, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=23.6842105263158, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50791; Mature: 50791

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHYLSMKLKLTLMFFSTSVFVFIGLGFAIQYLVQSHFYEEDLRLINANRTSIFVLLEN
CCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEEC
EGITSSEIFASLKSRGINIWIANDNGTLTQNTNVIPNDSAFKQSKKINEWTYAGSTFRTL
CCCCHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCCCHHHHHHCCCCCEEECCCEEEEE
YYPLENENNYTGLLIGINIDHHIEFNKRLKGILIWSISIASLLSFICSFIIVNMGFKPLQ
EEEECCCCCEEEEEEEEEECCCEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHH
YLQDRIKQVQPNKLSIRLSDEHLPNELVELAKIQNQMLDRLELGFERLSDFSSDIAHELK
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TPLSNIMTQTHVTLSQPRTPHEYQEILSSNLEELERINKTINDTLYLAKSENDLLLKDNM
HHHHHHHHHHEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCCCEEEECCC
QLDLEQLFAPIIEYYSIVSEETGVTIHLSGNGSLWGDKSMIQRVANNLLSNAIRHSAPDS
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NIQIKIEENHSNIVLSISNIGDKIPDKDLPFIFERFYRADKSRQHSSSTGAGLGLTIVRS
CEEEEEEECCCEEEEEEHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
IVNLHNGDISVQSEGKKTTFLLTFPL
HHHHCCCCEEEECCCCEEEEEEEECC
>Mature Secondary Structure
MKKHYLSMKLKLTLMFFSTSVFVFIGLGFAIQYLVQSHFYEEDLRLINANRTSIFVLLEN
CCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEEC
EGITSSEIFASLKSRGINIWIANDNGTLTQNTNVIPNDSAFKQSKKINEWTYAGSTFRTL
CCCCHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCCCHHHHHHCCCCCEEECCCEEEEE
YYPLENENNYTGLLIGINIDHHIEFNKRLKGILIWSISIASLLSFICSFIIVNMGFKPLQ
EEEECCCCCEEEEEEEEEECCCEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHH
YLQDRIKQVQPNKLSIRLSDEHLPNELVELAKIQNQMLDRLELGFERLSDFSSDIAHELK
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TPLSNIMTQTHVTLSQPRTPHEYQEILSSNLEELERINKTINDTLYLAKSENDLLLKDNM
HHHHHHHHHHEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCCCEEEECCC
QLDLEQLFAPIIEYYSIVSEETGVTIHLSGNGSLWGDKSMIQRVANNLLSNAIRHSAPDS
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NIQIKIEENHSNIVLSISNIGDKIPDKDLPFIFERFYRADKSRQHSSSTGAGLGLTIVRS
CEEEEEEECCCEEEEEEHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
IVNLHNGDISVQSEGKKTTFLLTFPL
HHHHCCCCEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9930866 [H]