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The map label for this gene is nemA [H]

Identifier: 59713301

GI number: 59713301

Start: 133199

End: 134302

Strand: Reverse

Name: nemA [H]

Synonym: VF_A0118

Alternate gene names: 59713301

Gene position: 134302-133199 (Counterclockwise)

Preceding gene: 59713302

Following gene: 59713300

Centisome position: 10.1

GC content: 43.03

Gene sequence:

>1104_bases
ATGAAAAATGCACTATTTCAACCAATGCAACTTGGTAATCTGACACTAAAAAACCGTATCGTTATGCCTCCTATGACTCG
TTCTCGTGCAAGCCAACCGGGTAATGTAGCGAACGATATGATGGCCAAATACTATGCTCAACGTGCAAGTGCAGGTCTTA
TTATCGCAGAGGGAACTCAAATTTCAGAAATGGGTCAAGGTTACGCATGGACTCCGGGAATTTATTCTCAAGAACAAATC
CTTGGTTGGAAAAAAGTAACTGATGCCGTTCATGCTAAAGGCGGTACTATTTTCGCTCAGCTTTGGCATGTAGGCCGTGT
CACTCACCCTGAAAACATTGGTGGTCAACAACCTATTTCTTCTTCTGCACTTAAAGCAGAGAACGTAAAGGTATTCATCG
ATAACGGTACTGATGCGCCAGGTTTTGTTGATGTTGTTGAGCCTCGTGAAATGACTCAGCAAGATATTAACGATGTTATC
GCAGAATATCGCCAAGCGGCATTAAACGCGATTGAAGCAGGCTTTGATGGTATTGAGCTTCACGCAGCCAATGGTTATTT
AATTAATCAATTCATTGACTCTGAAGCGAACAACCGTACCGATGAATACGGTGGTTCTATCGAAAATCGTCTGCGTTTTC
TAGGCGAAGTTGTCGAAGCAGTAAACGAAGCCATTGGTTACGACCGTGTTGGTGTTCGCCTTGCTCCATTTACTTCTCTA
AATGGCACAGTCGATAAAACACCAGTAGAAACCTACACAGCAGCAGCAGCATTATTAAACAAACACAACGTGGTTTATAT
GCACATTGCAGAAGTGGACTGGGATAATGCCCCTGAGACGCCTGAGGAATTTAAAACAGCAATTCGTGAGGCTTATCAAG
GCACGATTATTTATGCTGGTCGTTATAATACAGGCAAAGCGGCGCAAGCACTGACAGACAACCTTGCAGATCTAGTTGGA
TTTGGTCGCCCATTTATTGCGAATCCTGATTTGCCTAATCGTATTAAAAACGATTATCCATTAGCTGAGCACGATCCAAA
TACGCTATTTGGTGGTACACAACAAGGGCTTGTAGATTACCCTGAATACCAAGAAGCGCATTAA

Upstream 100 bases:

>100_bases
CTGGGCCAGTAAAAGGCGGTACACGCGTATTGCGTTTATTACAGATCCTGATGGTTATCAAATTGAGCTTATTCAATTAA
GCAAATAAGAGGATTTATTG

Downstream 100 bases:

>100_bases
ATTTAAAACAAATGAATCAACTACACATAACCCTGCTTTTTTGAGTGGGGTTATTTTTTATAAATGATGATAACACTGGT
ATTATCGTCATTTTTATCAA

Product: morphinone reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKNALFQPMQLGNLTLKNRIVMPPMTRSRASQPGNVANDMMAKYYAQRASAGLIIAEGTQISEMGQGYAWTPGIYSQEQI
LGWKKVTDAVHAKGGTIFAQLWHVGRVTHPENIGGQQPISSSALKAENVKVFIDNGTDAPGFVDVVEPREMTQQDINDVI
AEYRQAALNAIEAGFDGIELHAANGYLINQFIDSEANNRTDEYGGSIENRLRFLGEVVEAVNEAIGYDRVGVRLAPFTSL
NGTVDKTPVETYTAAAALLNKHNVVYMHIAEVDWDNAPETPEEFKTAIREAYQGTIIYAGRYNTGKAAQALTDNLADLVG
FGRPFIANPDLPNRIKNDYPLAEHDPNTLFGGTQQGLVDYPEYQEAH

Sequences:

>Translated_367_residues
MKNALFQPMQLGNLTLKNRIVMPPMTRSRASQPGNVANDMMAKYYAQRASAGLIIAEGTQISEMGQGYAWTPGIYSQEQI
LGWKKVTDAVHAKGGTIFAQLWHVGRVTHPENIGGQQPISSSALKAENVKVFIDNGTDAPGFVDVVEPREMTQQDINDVI
AEYRQAALNAIEAGFDGIELHAANGYLINQFIDSEANNRTDEYGGSIENRLRFLGEVVEAVNEAIGYDRVGVRLAPFTSL
NGTVDKTPVETYTAAAALLNKHNVVYMHIAEVDWDNAPETPEEFKTAIREAYQGTIIYAGRYNTGKAAQALTDNLADLVG
FGRPFIANPDLPNRIKNDYPLAEHDPNTLFGGTQQGLVDYPEYQEAH
>Mature_367_residues
MKNALFQPMQLGNLTLKNRIVMPPMTRSRASQPGNVANDMMAKYYAQRASAGLIIAEGTQISEMGQGYAWTPGIYSQEQI
LGWKKVTDAVHAKGGTIFAQLWHVGRVTHPENIGGQQPISSSALKAENVKVFIDNGTDAPGFVDVVEPREMTQQDINDVI
AEYRQAALNAIEAGFDGIELHAANGYLINQFIDSEANNRTDEYGGSIENRLRFLGEVVEAVNEAIGYDRVGVRLAPFTSL
NGTVDKTPVETYTAAAALLNKHNVVYMHIAEVDWDNAPETPEEFKTAIREAYQGTIIYAGRYNTGKAAQALTDNLADLVG
FGRPFIANPDLPNRIKNDYPLAEHDPNTLFGGTQQGLVDYPEYQEAH

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=43.0555555555556, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1789463, Length=365, Percent_Identity=29.041095890411, Blast_Score=127, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=257, Percent_Identity=32.6848249027237, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=290, Percent_Identity=30, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17540738, Length=237, Percent_Identity=32.4894514767932, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17559804, Length=268, Percent_Identity=29.8507462686567, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=257, Percent_Identity=32.295719844358, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=271, Percent_Identity=29.8892988929889, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=298, Percent_Identity=26.510067114094, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=386, Percent_Identity=39.119170984456, Blast_Score=238, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6325086, Length=384, Percent_Identity=37.7604166666667, Blast_Score=232, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40292; Mature: 40292

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNALFQPMQLGNLTLKNRIVMPPMTRSRASQPGNVANDMMAKYYAQRASAGLIIAEGTQ
CCCCCCCCHHHCCEEECCEEECCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCC
ISEMGQGYAWTPGIYSQEQILGWKKVTDAVHAKGGTIFAQLWHVGRVTHPENIGGQQPIS
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
SSALKAENVKVFIDNGTDAPGFVDVVEPREMTQQDINDVIAEYRQAALNAIEAGFDGIEL
CCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HAANGYLINQFIDSEANNRTDEYGGSIENRLRFLGEVVEAVNEAIGYDRVGVRLAPFTSL
EECCCEEEHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
NGTVDKTPVETYTAAAALLNKHNVVYMHIAEVDWDNAPETPEEFKTAIREAYQGTIIYAG
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEE
RYNTGKAAQALTDNLADLVGFGRPFIANPDLPNRIKNDYPLAEHDPNTLFGGTQQGLVDY
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
PEYQEAH
CCCCCCC
>Mature Secondary Structure
MKNALFQPMQLGNLTLKNRIVMPPMTRSRASQPGNVANDMMAKYYAQRASAGLIIAEGTQ
CCCCCCCCHHHCCEEECCEEECCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCC
ISEMGQGYAWTPGIYSQEQILGWKKVTDAVHAKGGTIFAQLWHVGRVTHPENIGGQQPIS
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
SSALKAENVKVFIDNGTDAPGFVDVVEPREMTQQDINDVIAEYRQAALNAIEAGFDGIEL
CCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HAANGYLINQFIDSEANNRTDEYGGSIENRLRFLGEVVEAVNEAIGYDRVGVRLAPFTSL
EECCCEEEHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
NGTVDKTPVETYTAAAALLNKHNVVYMHIAEVDWDNAPETPEEFKTAIREAYQGTIIYAG
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEE
RYNTGKAAQALTDNLADLVGFGRPFIANPDLPNRIKNDYPLAEHDPNTLFGGTQQGLVDY
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
PEYQEAH
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]