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The map label for this gene is nfnB [C]

Identifier: 59713296

GI number: 59713296

Start: 128447

End: 129106

Strand: Reverse

Name: nfnB [C]

Synonym: VF_A0113

Alternate gene names: 59713296

Gene position: 129106-128447 (Counterclockwise)

Preceding gene: 59713300

Following gene: 59713292

Centisome position: 9.7

GC content: 38.33

Gene sequence:

>660_bases
ATGACTCACCCAATTATTGCTGATTTAAATTCTCGTTATACTGCTAAAAAATACGACGCAACAAAGCGCATTTCAGCAGA
AGATATGGACGTAATTAAAGAAGCGATTCGTCTATCTGCATCTTCAATTAACTCTCAACCTTGGAAATTCATTGTTCTTG
AGAGTGAAGAAGCAAAAGAACGTTTTCATGGTACATTTGCGAATATGTTTGAATTTAATCAACCACACGCAAAAGAAGCA
TCGCACACGATTTTATTTGCACACAATCCAAACTATACAAAAGACCAATACCGTAAAGTAGTGGATGCTGAAGTTGCTTC
TGGCCACTTACCTGCTGAGCGTTATAACGATATGCTAGATGGCGCTTTTGGCTTTGTTGAATTAAATACTAACGAAGCAG
GCTTTAATGGTAACTGGACAAAAGCACAAAGCTACATTGCTCTAGGTAACACACTGCATACTCTTGCTCGTATGGGCATA
GCATCAACATCAATGGAAGGTGTTGACTCAGCATTGATTGGTGAAGAGTTTAAAGATGAATTAGATGGTTATGTATGTGA
TTTTGCCCTTGCGTTGGGTTATCACAAAGATGGCGAAGATTATAACCACGGTCTACCAAAAGCACGTTTACCAATGGAAG
ATGTGATTACAACACTTTAA

Upstream 100 bases:

>100_bases
TTTTTCTCGTCTTTGATTTTCTTTATCTTACTCACATCAACGACGAGTAAAGAAAATATAAACTCGCGCTTTTGTTCACC
AGATTTAGGAAATAGCTCAT

Downstream 100 bases:

>100_bases
TTTTCCCTAATGTAAATAAAAAAAGAGCACATAACCAATGTGCTCTTTTTTGTTTTTAGGGTAAGTCATCGTGTCTTACT
CTTTTTTACTAAATTCAACG

Product: NAD(P)H-dependent flavin reductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: FRASE I [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTHPIIADLNSRYTAKKYDATKRISAEDMDVIKEAIRLSASSINSQPWKFIVLESEEAKERFHGTFANMFEFNQPHAKEA
SHTILFAHNPNYTKDQYRKVVDAEVASGHLPAERYNDMLDGAFGFVELNTNEAGFNGNWTKAQSYIALGNTLHTLARMGI
ASTSMEGVDSALIGEEFKDELDGYVCDFALALGYHKDGEDYNHGLPKARLPMEDVITTL

Sequences:

>Translated_219_residues
MTHPIIADLNSRYTAKKYDATKRISAEDMDVIKEAIRLSASSINSQPWKFIVLESEEAKERFHGTFANMFEFNQPHAKEA
SHTILFAHNPNYTKDQYRKVVDAEVASGHLPAERYNDMLDGAFGFVELNTNEAGFNGNWTKAQSYIALGNTLHTLARMGI
ASTSMEGVDSALIGEEFKDELDGYVCDFALALGYHKDGEDYNHGLPKARLPMEDVITTL
>Mature_218_residues
THPIIADLNSRYTAKKYDATKRISAEDMDVIKEAIRLSASSINSQPWKFIVLESEEAKERFHGTFANMFEFNQPHAKEAS
HTILFAHNPNYTKDQYRKVVDAEVASGHLPAERYNDMLDGAFGFVELNTNEAGFNGNWTKAQSYIALGNTLHTLARMGIA
STSMEGVDSALIGEEFKDELDGYVCDFALALGYHKDGEDYNHGLPKARLPMEDVITTL

Specific function: Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction. Major FMN reductase. It is capable of using both NADH and NADPH as electron donors. As electron acceptor, FMN is the most effectiv

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=216, Percent_Identity=32.8703703703704, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24501; Mature: 24369

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPIIADLNSRYTAKKYDATKRISAEDMDVIKEAIRLSASSINSQPWKFIVLESEEAKE
CCCCEEECCCCCCCHHHHCHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHH
RFHGTFANMFEFNQPHAKEASHTILFAHNPNYTKDQYRKVVDAEVASGHLPAERYNDMLD
HHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHC
GAFGFVELNTNEAGFNGNWTKAQSYIALGNTLHTLARMGIASTSMEGVDSALIGEEFKDE
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LDGYVCDFALALGYHKDGEDYNHGLPKARLPMEDVITTL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
THPIIADLNSRYTAKKYDATKRISAEDMDVIKEAIRLSASSINSQPWKFIVLESEEAKE
CCCEEECCCCCCCHHHHCHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHH
RFHGTFANMFEFNQPHAKEASHTILFAHNPNYTKDQYRKVVDAEVASGHLPAERYNDMLD
HHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHC
GAFGFVELNTNEAGFNGNWTKAQSYIALGNTLHTLARMGIASTSMEGVDSALIGEEFKDE
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LDGYVCDFALALGYHKDGEDYNHGLPKARLPMEDVITTL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8206830; 8033996; 9654450 [H]