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The map label for this gene is yeiT [H]

Identifier: 59713283

GI number: 59713283

Start: 111392

End: 113188

Strand: Reverse

Name: yeiT [H]

Synonym: VF_A0100

Alternate gene names: 59713283

Gene position: 113188-111392 (Counterclockwise)

Preceding gene: 59713284

Following gene: 59713282

Centisome position: 8.51

GC content: 43.46

Gene sequence:

>1797_bases
ATGAGCAATGTAATTTTTAGTCAAAAACTCTACGATACCGCCCAAATGGTCGTAGATGGCTGCATGGGTGATGCCGACCC
AGCATGTCAAACTGCATGTCCAATGCACACGGATGTGAAACAATATGTACGCTTAGCGGGTGAAGGTAACTACCAAGAAG
CTTTAGATCTGATCCGCGGCAAACTTTTCTTACCTCAAACTCTTGGCCGTATTTGTGCTCATCCATGTGAAGCAGCATGT
CGTCGTAATACTGAGTATAACCAACCAATCAGTGTTGCAGGGATCAAACGCTTTGTTGCCGAAAAAATGGATAAACAAAG
CAATTGGGATCTGACCGTTGCCAATGACAGCGGTAAAAAAATCGCTATTGTTGGTGCTGGCCCCGCTGGTGCACAAGCAG
CGATTGAGCTTCGCCGTAGCGGTCACCAAGTGACGATCTATGAAAAGCTGAATGTTTACGGTGGCATGATGCGTGTGGGT
ATTCCTGAATACCGTTTACCGCGCGATGTTATCGATTTTGAATACGGCTATTTAGATATGTTAGGTATTGAAACGAAATT
CGGAATAGAGATAGGTAAAGACATCTCTTTCAATGAGCTTCGTCTTTCTCATGACGCTATTATTTTAGCTCACGGTGCTC
ATGTTGGTTCAATCATTCCGCTTCCAGGCCACAAAGCGCAAGGCGTATTCTCTGCGGTTGAATATCTAAAAGAAGTATCA
GAAACTCGTGATTTCCCTCGTGCAGGTAAGCGAATCATGGTCATTGGCGGCGGAGACGTTGCTATGGACTGTGCTCGTTC
ATCATGGCGCATCGGTGTAGATGAAGTACACCAATGTTCATTAGAAGAAATGGCGAAACTGCCTGCAAGCCAAATTGAAA
TTGATGAATCTCTAGAAGAAGGCGTGTTATTTAATGCTGGCTGGGGACCGATTTCAATTGAAGAAACAGACGGAAAAGTA
ACTGGAATCGTACTTAAAAAAGTCATTTCTATTTTTGATGAAAATGGCAACTTTTCACCAAGTTACTCGGATGAAACCCG
TACGATCGCTGTAGATACCGTTATTTTCGCAACAGGACAAGTAGTAGCAGACATTACTGATGGTGCTCTTGAACAAACTC
GTGGCGGCCGTTACGTGGTTGATAAAGATACCCTAGCGTCTTCCGTTGACGGTGTTTACGTTGCTGGTGATGCCTGTGGC
GGTAACATTGTTGTTGAGGCAATGGCACTAGGTCATAAAGCGGCACTGAGTATTGAGCGTCAATTCGCTAAGCGTCCTCT
TACTGAAAATCGTGATTTTGAGAAAGAGTACAACTACCCTTCTCGTCTTGATATTCCGCTTCCAAAAGGCACGACCGATA
CACCTCGCCTACATGGTCAATTACGTGATGCTGAAGAACGTAAGCAAGATTTTAAACAAGTTGATCTTGGTTTTTCAGAA
GAACAAATCAAGCAAGAAGCGTCTCGCTGTCTGCAATGTACTTGTAAATTGTGCATGAATGAATGTGTGATGATGAATGA
CTTTGGCGATTGCCCTAAAACCTTGTTTAGTGATTTCATTAATAACAAAACCATGGATCCCCTACTGGCTTATTCATGTA
ATGCCTGTGACCAATGTACGATTGTTTGTCCTAAAGACTACCCAATGAAAGAAATGTTCTTAAGTGCTCGTGTGGATTTT
GTTAATGCCAATGGCGGTAACTCTCCAATCCCGGGACACAAAGCAATCAATATGCACCAGAAGCTTGGCTTCTCTAAATT
CTTCACTATGGCTAAACGTACGGTGGCTTCAAAATGA

Upstream 100 bases:

>100_bases
GGATTGAGTACAGCCACGCTCACTATGAAGAAAAACAGCCGAACGTTACCGTTATCAACGGCCAAATCTAATTCAATCTT
AAATTGGAATCATAATAAAA

Downstream 100 bases:

>100_bases
GTAATAAAGTATTTATGCCAGGATGCAGTCTGCCATCCTACTCGCCAGAAGGCGTTGCAAGCATCGCAAAATATCTAAAA
GAAGTGTACCCAGATATGGG

Product: oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MSNVIFSQKLYDTAQMVVDGCMGDADPACQTACPMHTDVKQYVRLAGEGNYQEALDLIRGKLFLPQTLGRICAHPCEAAC
RRNTEYNQPISVAGIKRFVAEKMDKQSNWDLTVANDSGKKIAIVGAGPAGAQAAIELRRSGHQVTIYEKLNVYGGMMRVG
IPEYRLPRDVIDFEYGYLDMLGIETKFGIEIGKDISFNELRLSHDAIILAHGAHVGSIIPLPGHKAQGVFSAVEYLKEVS
ETRDFPRAGKRIMVIGGGDVAMDCARSSWRIGVDEVHQCSLEEMAKLPASQIEIDESLEEGVLFNAGWGPISIEETDGKV
TGIVLKKVISIFDENGNFSPSYSDETRTIAVDTVIFATGQVVADITDGALEQTRGGRYVVDKDTLASSVDGVYVAGDACG
GNIVVEAMALGHKAALSIERQFAKRPLTENRDFEKEYNYPSRLDIPLPKGTTDTPRLHGQLRDAEERKQDFKQVDLGFSE
EQIKQEASRCLQCTCKLCMNECVMMNDFGDCPKTLFSDFINNKTMDPLLAYSCNACDQCTIVCPKDYPMKEMFLSARVDF
VNANGGNSPIPGHKAINMHQKLGFSKFFTMAKRTVASK

Sequences:

>Translated_598_residues
MSNVIFSQKLYDTAQMVVDGCMGDADPACQTACPMHTDVKQYVRLAGEGNYQEALDLIRGKLFLPQTLGRICAHPCEAAC
RRNTEYNQPISVAGIKRFVAEKMDKQSNWDLTVANDSGKKIAIVGAGPAGAQAAIELRRSGHQVTIYEKLNVYGGMMRVG
IPEYRLPRDVIDFEYGYLDMLGIETKFGIEIGKDISFNELRLSHDAIILAHGAHVGSIIPLPGHKAQGVFSAVEYLKEVS
ETRDFPRAGKRIMVIGGGDVAMDCARSSWRIGVDEVHQCSLEEMAKLPASQIEIDESLEEGVLFNAGWGPISIEETDGKV
TGIVLKKVISIFDENGNFSPSYSDETRTIAVDTVIFATGQVVADITDGALEQTRGGRYVVDKDTLASSVDGVYVAGDACG
GNIVVEAMALGHKAALSIERQFAKRPLTENRDFEKEYNYPSRLDIPLPKGTTDTPRLHGQLRDAEERKQDFKQVDLGFSE
EQIKQEASRCLQCTCKLCMNECVMMNDFGDCPKTLFSDFINNKTMDPLLAYSCNACDQCTIVCPKDYPMKEMFLSARVDF
VNANGGNSPIPGHKAINMHQKLGFSKFFTMAKRTVASK
>Mature_597_residues
SNVIFSQKLYDTAQMVVDGCMGDADPACQTACPMHTDVKQYVRLAGEGNYQEALDLIRGKLFLPQTLGRICAHPCEAACR
RNTEYNQPISVAGIKRFVAEKMDKQSNWDLTVANDSGKKIAIVGAGPAGAQAAIELRRSGHQVTIYEKLNVYGGMMRVGI
PEYRLPRDVIDFEYGYLDMLGIETKFGIEIGKDISFNELRLSHDAIILAHGAHVGSIIPLPGHKAQGVFSAVEYLKEVSE
TRDFPRAGKRIMVIGGGDVAMDCARSSWRIGVDEVHQCSLEEMAKLPASQIEIDESLEEGVLFNAGWGPISIEETDGKVT
GIVLKKVISIFDENGNFSPSYSDETRTIAVDTVIFATGQVVADITDGALEQTRGGRYVVDKDTLASSVDGVYVAGDACGG
NIVVEAMALGHKAALSIERQFAKRPLTENRDFEKEYNYPSRLDIPLPKGTTDTPRLHGQLRDAEERKQDFKQVDLGFSEE
QIKQEASRCLQCTCKLCMNECVMMNDFGDCPKTLFSDFINNKTMDPLLAYSCNACDQCTIVCPKDYPMKEMFLSARVDFV
NANGGNSPIPGHKAINMHQKLGFSKFFTMAKRTVASK

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=434, Percent_Identity=28.110599078341, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1789606, Length=429, Percent_Identity=30.5361305361305, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788811, Length=431, Percent_Identity=29.2343387470998, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1789243, Length=551, Percent_Identity=28.8566243194192, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87082180, Length=432, Percent_Identity=29.8611111111111, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788468, Length=402, Percent_Identity=30.3482587064677, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17570289, Length=440, Percent_Identity=27.9545454545455, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71984108, Length=435, Percent_Identity=28.9655172413793, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17543860, Length=171, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=449, Percent_Identity=24.9443207126949, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640763, Length=433, Percent_Identity=29.0993071593533, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI18858217, Length=433, Percent_Identity=29.0993071593533, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24665547, Length=440, Percent_Identity=25.4545454545455, Blast_Score=141, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24665543, Length=440, Percent_Identity=25.4545454545455, Blast_Score=141, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574881, Length=441, Percent_Identity=25.6235827664399, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24665539, Length=441, Percent_Identity=25.6235827664399, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 65794; Mature: 65663

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVIFSQKLYDTAQMVVDGCMGDADPACQTACPMHTDVKQYVRLAGEGNYQEALDLIRG
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHC
KLFLPQTLGRICAHPCEAACRRNTEYNQPISVAGIKRFVAEKMDKQSNWDLTVANDSGKK
CCCCHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCE
IAIVGAGPAGAQAAIELRRSGHQVTIYEKLNVYGGMMRVGIPEYRLPRDVIDFEYGYLDM
EEEEECCCCCHHHHHHHHHCCCEEEEEEECCHHCCCCCCCCCCCCCCHHHHHCCCCEEEE
LGIETKFGIEIGKDISFNELRLSHDAIILAHGAHVGSIIPLPGHKAQGVFSAVEYLKEVS
ECCCEEECCEECCCCCHHHEEECCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHH
ETRDFPRAGKRIMVIGGGDVAMDCARSSWRIGVDEVHQCSLEEMAKLPASQIEIDESLEE
HHCCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHCCHHHHHCCCHHHEEHHHHHHC
GVLFNAGWGPISIEETDGKVTGIVLKKVISIFDENGNFSPSYSDETRTIAVDTVIFATGQ
CEEEECCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCC
VVADITDGALEQTRGGRYVVDKDTLASSVDGVYVAGDACGGNIVVEAMALGHKAALSIER
EEEECCCHHHHHCCCCEEEEECHHHHHCCCCEEEECCCCCCCEEEHHHHHCCHHHHHHHH
QFAKRPLTENRDFEKEYNYPSRLDIPLPKGTTDTPRLHGQLRDAEERKQDFKQVDLGFSE
HHHHCCCCCCCCCHHHCCCCCEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCH
EQIKQEASRCLQCTCKLCMNECVMMNDFGDCPKTLFSDFINNKTMDPLLAYSCNACDQCT
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCEE
IVCPKDYPMKEMFLSARVDFVNANGGNSPIPGHKAINMHQKLGFSKFFTMAKRTVASK
EECCCCCCHHHHHHHHHEEEEECCCCCCCCCCCHHCCHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNVIFSQKLYDTAQMVVDGCMGDADPACQTACPMHTDVKQYVRLAGEGNYQEALDLIRG
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHC
KLFLPQTLGRICAHPCEAACRRNTEYNQPISVAGIKRFVAEKMDKQSNWDLTVANDSGKK
CCCCHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCE
IAIVGAGPAGAQAAIELRRSGHQVTIYEKLNVYGGMMRVGIPEYRLPRDVIDFEYGYLDM
EEEEECCCCCHHHHHHHHHCCCEEEEEEECCHHCCCCCCCCCCCCCCHHHHHCCCCEEEE
LGIETKFGIEIGKDISFNELRLSHDAIILAHGAHVGSIIPLPGHKAQGVFSAVEYLKEVS
ECCCEEECCEECCCCCHHHEEECCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHH
ETRDFPRAGKRIMVIGGGDVAMDCARSSWRIGVDEVHQCSLEEMAKLPASQIEIDESLEE
HHCCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHCCHHHHHCCCHHHEEHHHHHHC
GVLFNAGWGPISIEETDGKVTGIVLKKVISIFDENGNFSPSYSDETRTIAVDTVIFATGQ
CEEEECCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCC
VVADITDGALEQTRGGRYVVDKDTLASSVDGVYVAGDACGGNIVVEAMALGHKAALSIER
EEEECCCHHHHHCCCCEEEEECHHHHHCCCCEEEECCCCCCCEEEHHHHHCCHHHHHHHH
QFAKRPLTENRDFEKEYNYPSRLDIPLPKGTTDTPRLHGQLRDAEERKQDFKQVDLGFSE
HHHHCCCCCCCCCHHHCCCCCEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCH
EQIKQEASRCLQCTCKLCMNECVMMNDFGDCPKTLFSDFINNKTMDPLLAYSCNACDQCT
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCEE
IVCPKDYPMKEMFLSARVDFVNANGGNSPIPGHKAINMHQKLGFSKFFTMAKRTVASK
EECCCCCCHHHHHHHHHEEEEECCCCCCCCCCCHHCCHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]