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The map label for this gene is cysA [H]

Identifier: 59713284

GI number: 59713284

Start: 113218

End: 114201

Strand: Reverse

Name: cysA [H]

Synonym: VF_A0101

Alternate gene names: 59713284

Gene position: 114201-113218 (Counterclockwise)

Preceding gene: 59713289

Following gene: 59713283

Centisome position: 8.58

GC content: 42.99

Gene sequence:

>984_bases
ATGAAAAAATCAACAATTGCCATCGGCGTTTTAGCCGCTGCGGTCGCAATAGGGTACCCAACTTACAAATCCATGTTTTC
AGCTCCCGCTTTTGAGATCAGTAAACAACACCTAACTGAAAAATTCTCTCATTACGCTCATCCTGAGCACTTTATCGCAG
CTCAAGATCTAAAAGAGATGATGGATAACGGTGATAATGTTGTGGTTATTGGTGCATTAAATCCAATGAAACCTGACGCT
CCTATTGAAGGCTCTTTTACCATGTGGCGTGATGGTTACTCGGCAAAAGAAGGCACTTATGAGTTCGATGGCATGAGTAA
CACAACCGAAGAGATGGAAAAAATTCTAAGCTCTTATGGTGCAACACCTGAATCAACTATCGTTGTTTATGCAGCAACAT
CACACCACGATGCTGCTCGTCTATTTTGGCAAATTGAAGCACTAGGCCACAGCGATATTCGCTACCTAGACGGCGGCTTA
AACGCTTGGGTTGGTGCTGATTTACCAACAGGCAATGCAAATCCATCGGTTGCTCAAAGTGATTATCAAGCATCACAACC
TGCACATAAACACACAGCTCTTGCTACGTTAGATATGGTGATTGAAGCTCAAAACAATGATGAGTGGGTAATCATTGATA
CGCGTGGTAACGACGAATTTAATGGCACAACAACGGTTGGTGGCGCATTTGGACCAGGAACGATTCCAAAAAGCGTACAC
ATTAACTGGACTAAAGCAGTAAACGAAGACACCACGTTAAAATCAGCAGAAGAATTACAAGCGCTATACGGCGACATTAT
TAAAGGCAAAAAAGTCATTGCCTACTGTCAATCAGGCGTTCGCTCTGCACATACAACCATGATTCTACAAAACGTACTTG
GTGCAGAAGAAGTTTACAACTATGACGGCTCATGGATTGAGTACAGCCACGCTCACTATGAAGAAAAACAGCCGAACGTT
ACCGTTATCAACGGCCAAATCTAA

Upstream 100 bases:

>100_bases
TAGCGGCCACTTCCTCCAGTTTTAGGTTCAAAAATTCAATCTGTTTTGTTTATACCGATGAACAAAACATAAAAACTTTA
AACTTACTGGAAAATACACA

Downstream 100 bases:

>100_bases
TTCAATCTTAAATTGGAATCATAATAAAAATGAGCAATGTAATTTTTAGTCAAAAACTCTACGATACCGCCCAAATGGTC
GTAGATGGCTGCATGGGTGA

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKKSTIAIGVLAAAVAIGYPTYKSMFSAPAFEISKQHLTEKFSHYAHPEHFIAAQDLKEMMDNGDNVVVIGALNPMKPDA
PIEGSFTMWRDGYSAKEGTYEFDGMSNTTEEMEKILSSYGATPESTIVVYAATSHHDAARLFWQIEALGHSDIRYLDGGL
NAWVGADLPTGNANPSVAQSDYQASQPAHKHTALATLDMVIEAQNNDEWVIIDTRGNDEFNGTTTVGGAFGPGTIPKSVH
INWTKAVNEDTTLKSAEELQALYGDIIKGKKVIAYCQSGVRSAHTTMILQNVLGAEEVYNYDGSWIEYSHAHYEEKQPNV
TVINGQI

Sequences:

>Translated_327_residues
MKKSTIAIGVLAAAVAIGYPTYKSMFSAPAFEISKQHLTEKFSHYAHPEHFIAAQDLKEMMDNGDNVVVIGALNPMKPDA
PIEGSFTMWRDGYSAKEGTYEFDGMSNTTEEMEKILSSYGATPESTIVVYAATSHHDAARLFWQIEALGHSDIRYLDGGL
NAWVGADLPTGNANPSVAQSDYQASQPAHKHTALATLDMVIEAQNNDEWVIIDTRGNDEFNGTTTVGGAFGPGTIPKSVH
INWTKAVNEDTTLKSAEELQALYGDIIKGKKVIAYCQSGVRSAHTTMILQNVLGAEEVYNYDGSWIEYSHAHYEEKQPNV
TVINGQI
>Mature_327_residues
MKKSTIAIGVLAAAVAIGYPTYKSMFSAPAFEISKQHLTEKFSHYAHPEHFIAAQDLKEMMDNGDNVVVIGALNPMKPDA
PIEGSFTMWRDGYSAKEGTYEFDGMSNTTEEMEKILSSYGATPESTIVVYAATSHHDAARLFWQIEALGHSDIRYLDGGL
NAWVGADLPTGNANPSVAQSDYQASQPAHKHTALATLDMVIEAQNNDEWVIIDTRGNDEFNGTTTVGGAFGPGTIPKSVH
INWTKAVNEDTTLKSAEELQALYGDIIKGKKVIAYCQSGVRSAHTTMILQNVLGAEEVYNYDGSWIEYSHAHYEEKQPNV
TVINGQI

Specific function: May be a sulfotransferase involved in the formation of thiosulfate [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=234, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI61835204, Length=234, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI194473681, Length=234, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI17402865, Length=193, Percent_Identity=25.9067357512953, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI87081967, Length=216, Percent_Identity=26.3888888888889, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87082121, Length=207, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 35740; Mature: 35740

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSTIAIGVLAAAVAIGYPTYKSMFSAPAFEISKQHLTEKFSHYAHPEHFIAAQDLKEM
CCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MDNGDNVVVIGALNPMKPDAPIEGSFTMWRDGYSAKEGTYEFDGMSNTTEEMEKILSSYG
HHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHC
ATPESTIVVYAATSHHDAARLFWQIEALGHSDIRYLDGGLNAWVGADLPTGNANPSVAQS
CCCCCEEEEEEECCCCCHHEEEEEEECCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCC
DYQASQPAHKHTALATLDMVIEAQNNDEWVIIDTRGNDEFNGTTTVGGAFGPGTIPKSVH
CCCCCCCCHHHHHHEEEEEEEEECCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCEEE
INWTKAVNEDTTLKSAEELQALYGDIIKGKKVIAYCQSGVRSAHTTMILQNVLGAEEVYN
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHC
YDGSWIEYSHAHYEEKQPNVTVINGQI
CCCCEEEECCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MKKSTIAIGVLAAAVAIGYPTYKSMFSAPAFEISKQHLTEKFSHYAHPEHFIAAQDLKEM
CCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MDNGDNVVVIGALNPMKPDAPIEGSFTMWRDGYSAKEGTYEFDGMSNTTEEMEKILSSYG
HHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHC
ATPESTIVVYAATSHHDAARLFWQIEALGHSDIRYLDGGLNAWVGADLPTGNANPSVAQS
CCCCCEEEEEEECCCCCHHEEEEEEECCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCC
DYQASQPAHKHTALATLDMVIEAQNNDEWVIIDTRGNDEFNGTTTVGGAFGPGTIPKSVH
CCCCCCCCHHHHHHEEEEEEEEECCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCEEE
INWTKAVNEDTTLKSAEELQALYGDIIKGKKVIAYCQSGVRSAHTTMILQNVLGAEEVYN
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHC
YDGSWIEYSHAHYEEKQPNVTVINGQI
CCCCEEEECCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2294090 [H]