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The map label for this gene is yafC [C]

Identifier: 59713214

GI number: 59713214

Start: 33115

End: 33999

Strand: Reverse

Name: yafC [C]

Synonym: VF_A0031

Alternate gene names: 59713214

Gene position: 33999-33115 (Counterclockwise)

Preceding gene: 59713216

Following gene: 59713211

Centisome position: 2.56

GC content: 39.55

Gene sequence:

>885_bases
ATGGATCAGTTAAGTGCAATGAGAGCCTTTGTTCGAGTCGTCGAAACAGGCAGCTTTTCAGCTACAGGTAAAGAGATGAA
TACCTCTCAAACCACCATAAGTAAAAAAGTCGCGGCGCTTGAAAATCAAATAGGCGTAAAACTGTTAACTCGAAGCAGCA
GAGATCATTCGTTAACTCCTGCCGGAAGTGATTATTATCAGACCTGCGTTAATATTCTAAGTGAACTTGATGAAGTAGAA
GCCAAGGTCCGCTCTAAAGCAACACTGCCTCAAGGAAGTATAAGAATCACCGCACCCGTTGCTTTTGGTCGCTTTCTTCT
TGCTCCTATCGTTGCTGAATTCTTCCAACATTATCCTGATATTAAAATTGATTTACTTCTCAATGATAAGCACGTGAATT
TAATTAGTGAAGGTATTGATGTCGCTATTAGAGCAAAAAATCTCGAAGATTCCTCTTTAGTTGCCCGTCATCTTTTGGAT
AATCCTATGTACTTATTCGCGTCACCTGATTACTTAGAACAATATGGTGAGCCTAAACATCCGAACGAACTCTCCACACA
TAACTGCTTAATTTATTCAGGCCTACAAGATGCGCATATATGGAATTTTAATCATGGAGAAAAAGAATATTCAGTGCCTG
TAAAGGGAAATTTTCAAAGCGATAATGGCGATGTATTGTTAGATGTGGCGCTTACAGGAGTGGGCATTGTTTCTTTACCT
ATTTGGATGGCTAAAGCGCACTTAACATCGGGACATTTACGCCAGATAATGACAAAGTATTCAGGTCAACAGTTACCTAT
CAATGCCATCTACCCACAACGTCGTTATACGCCACTCAAAGTGCGCTGTTTTATTGATTTTTTAAAACAAAAGCTGTCTG
AGTAA

Upstream 100 bases:

>100_bases
GTATTTATTGCTTGAAAGAGAGTATAGACATAAACAAATTAGACTAGAACCATGCTAAAATGACTACCAAGTATGCGTTT
TTGGAATAATTGAGTAACCT

Downstream 100 bases:

>100_bases
TAACTTCTTCTAATCATACCTAACTTATCAAAGAGAGTAAGCGCTAACTTAAGTAAATTAGCGCTTTCATTGATTTTATG
TCTAACTACTTTTCTTGAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDQLSAMRAFVRVVETGSFSATGKEMNTSQTTISKKVAALENQIGVKLLTRSSRDHSLTPAGSDYYQTCVNILSELDEVE
AKVRSKATLPQGSIRITAPVAFGRFLLAPIVAEFFQHYPDIKIDLLLNDKHVNLISEGIDVAIRAKNLEDSSLVARHLLD
NPMYLFASPDYLEQYGEPKHPNELSTHNCLIYSGLQDAHIWNFNHGEKEYSVPVKGNFQSDNGDVLLDVALTGVGIVSLP
IWMAKAHLTSGHLRQIMTKYSGQQLPINAIYPQRRYTPLKVRCFIDFLKQKLSE

Sequences:

>Translated_294_residues
MDQLSAMRAFVRVVETGSFSATGKEMNTSQTTISKKVAALENQIGVKLLTRSSRDHSLTPAGSDYYQTCVNILSELDEVE
AKVRSKATLPQGSIRITAPVAFGRFLLAPIVAEFFQHYPDIKIDLLLNDKHVNLISEGIDVAIRAKNLEDSSLVARHLLD
NPMYLFASPDYLEQYGEPKHPNELSTHNCLIYSGLQDAHIWNFNHGEKEYSVPVKGNFQSDNGDVLLDVALTGVGIVSLP
IWMAKAHLTSGHLRQIMTKYSGQQLPINAIYPQRRYTPLKVRCFIDFLKQKLSE
>Mature_294_residues
MDQLSAMRAFVRVVETGSFSATGKEMNTSQTTISKKVAALENQIGVKLLTRSSRDHSLTPAGSDYYQTCVNILSELDEVE
AKVRSKATLPQGSIRITAPVAFGRFLLAPIVAEFFQHYPDIKIDLLLNDKHVNLISEGIDVAIRAKNLEDSSLVARHLLD
NPMYLFASPDYLEQYGEPKHPNELSTHNCLIYSGLQDAHIWNFNHGEKEYSVPVKGNFQSDNGDVLLDVALTGVGIVSLP
IWMAKAHLTSGHLRQIMTKYSGQQLPINAIYPQRRYTPLKVRCFIDFLKQKLSE

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=282, Percent_Identity=33.3333333333333, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1789440, Length=283, Percent_Identity=32.5088339222615, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=33.2203389830508, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=30.5369127516779, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=30.8219178082192, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=29.3103448275862, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787589, Length=295, Percent_Identity=27.4576271186441, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790011, Length=284, Percent_Identity=26.7605633802817, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=23.5099337748344, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787601, Length=171, Percent_Identity=27.4853801169591, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789173, Length=250, Percent_Identity=24, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=33.6206896551724, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693105, Length=174, Percent_Identity=26.4367816091954, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788887, Length=316, Percent_Identity=25.9493670886076, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788748, Length=175, Percent_Identity=29.7142857142857, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787879, Length=123, Percent_Identity=32.520325203252, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786448, Length=255, Percent_Identity=23.1372549019608, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32890; Mature: 32890

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQLSAMRAFVRVVETGSFSATGKEMNTSQTTISKKVAALENQIGVKLLTRSSRDHSLTP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
AGSDYYQTCVNILSELDEVEAKVRSKATLPQGSIRITAPVAFGRFLLAPIVAEFFQHYPD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCC
IKIDLLLNDKHVNLISEGIDVAIRAKNLEDSSLVARHLLDNPMYLFASPDYLEQYGEPKH
CEEEEEECCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCCC
PNELSTHNCLIYSGLQDAHIWNFNHGEKEYSVPVKGNFQSDNGDVLLDVALTGVGIVSLP
CCCCCCCCEEEEECCCCCEEECCCCCCEEEECCCCCCCCCCCCCEEEEEEHHCCHHHHHH
IWMAKAHLTSGHLRQIMTKYSGQQLPINAIYPQRRYTPLKVRCFIDFLKQKLSE
HHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDQLSAMRAFVRVVETGSFSATGKEMNTSQTTISKKVAALENQIGVKLLTRSSRDHSLTP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
AGSDYYQTCVNILSELDEVEAKVRSKATLPQGSIRITAPVAFGRFLLAPIVAEFFQHYPD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCC
IKIDLLLNDKHVNLISEGIDVAIRAKNLEDSSLVARHLLDNPMYLFASPDYLEQYGEPKH
CEEEEEECCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCCC
PNELSTHNCLIYSGLQDAHIWNFNHGEKEYSVPVKGNFQSDNGDVLLDVALTGVGIVSLP
CCCCCCCCEEEEECCCCCEEECCCCCCEEEECCCCCCCCCCCCCEEEEEEHHCCHHHHHH
IWMAKAHLTSGHLRQIMTKYSGQQLPINAIYPQRRYTPLKVRCFIDFLKQKLSE
HHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]