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The map label for this gene is fadH [C]

Identifier: 59713210

GI number: 59713210

Start: 28398

End: 29642

Strand: Reverse

Name: fadH [C]

Synonym: VF_A0027

Alternate gene names: 59713210

Gene position: 29642-28398 (Counterclockwise)

Preceding gene: 59713211

Following gene: 59713208

Centisome position: 2.23

GC content: 41.37

Gene sequence:

>1245_bases
ATGAATTCGATAAATCTCAACCAGCCATTTACTCTTACCAATGGTCAAGTTATTAAAAACCGTCTTTTCAAATCGGCAAT
GAGTGAACAGCTTGGCGATAAACTGCATAACCCTACTTCTGGTTTGGTAACCTTATACGCGCGTTGGGCTAAAGGTGGTA
TTGGTTTATCGATGACAGGCAATGTCATGGTGGATAGAACCGCCCTTGGCGAGCCTAAGAATGTGGTTTTAGATGATAAA
AGTGACCTTAGATTATTTAGTGAATGGGCAAAGGCAGGCACGCAAAATAGCTCACACATATGGATGCAACTTAACCATCC
AGGTAAGCAAATTCCAAAATTTCTATGTGCTAACCCTGTTGCTCCCTCAGCTATCTCACTAGAGCGTGGGTTAGAAAAAG
GCTTTAATACTCCACGAGCCCTCACTGAGAGTGAAATCCATGAGATCATTACTAAATTCGCTACCAGTGCCAAACTAGCA
AAGCAAGCAGGCTTTACTGGAGTACAAATTCATGGTGCTCATGGTTATCTTGTTAGTCAATTTTTATCCTCAAGGCACAA
TAAACGAAACGACAAATGGGGTGGTTCATTAGAAAACCGACTGCGTTTCGTACTCGAAGTATATAATGCTATTCGTAAAG
AAGTTGGGGCTGAATTCCCGGTAGGCATTAAGCTTAATAGTGCCGATTTTATGAAAGGCGGTTTCACTGAAAAAGAATCA
ATGCAGGTTGTGCAAGCCTTAAGTAATAATGGCATCGACTTAATTGAGATATCTGGCGGCACTTACGAGAGTCCATCAAT
GATGGGAGCCAAAGATAAAAATAAACCAGTAAAAGAAAGTACCGTTAAACGTGAAGCTTACTTTATGGATTACATGGAAA
AAGTAAGAACACTAGTTAATACACCTTTGGTTGTGACTGGTGGTTTTCGCTCTGCCCCTGCAATGAATGAAGCCTTAAAT
ACCTCTGCGACTGACTTTATCGGTATTGCACGAACCATGGCTGTTGACCCTGATTTCCCAAATAAATTAATGCACGATTC
AAACTATGGAATGCCGTTAGCAGAACCAACAACAGGCAAACCTGCATTAGATAAAATGGCGATGGTAGGACTTGTTTGGT
ATGAGCATCAAATGTGGCGTATTGCAGATGGAAAGGACGCTGACCCTCAATTGAGTGCATTTAGTGTGGTACTTAAAACT
TTACTAAGCGCCGGTTGGTATGCTTTTAAAAAGCGCAGAGCGTAA

Upstream 100 bases:

>100_bases
TTACATTTATATCGATAAAATAGTGCATACTAACTAGGGCGTGTTGATCTTTTATGATTAACACGCCCTAATCACATAAT
AATATAACCATAAGGCACGT

Downstream 100 bases:

>100_bases
GCAAAAAAGCCGAGTGTATATCTCACTCGGCTTTAGATTTTCAATATGCTCTTAAAGCATTACTTTCTTATTTGCCTTTC
ACTTTACTCTTCAAATTCTG

Product: NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MNSINLNQPFTLTNGQVIKNRLFKSAMSEQLGDKLHNPTSGLVTLYARWAKGGIGLSMTGNVMVDRTALGEPKNVVLDDK
SDLRLFSEWAKAGTQNSSHIWMQLNHPGKQIPKFLCANPVAPSAISLERGLEKGFNTPRALTESEIHEIITKFATSAKLA
KQAGFTGVQIHGAHGYLVSQFLSSRHNKRNDKWGGSLENRLRFVLEVYNAIRKEVGAEFPVGIKLNSADFMKGGFTEKES
MQVVQALSNNGIDLIEISGGTYESPSMMGAKDKNKPVKESTVKREAYFMDYMEKVRTLVNTPLVVTGGFRSAPAMNEALN
TSATDFIGIARTMAVDPDFPNKLMHDSNYGMPLAEPTTGKPALDKMAMVGLVWYEHQMWRIADGKDADPQLSAFSVVLKT
LLSAGWYAFKKRRA

Sequences:

>Translated_414_residues
MNSINLNQPFTLTNGQVIKNRLFKSAMSEQLGDKLHNPTSGLVTLYARWAKGGIGLSMTGNVMVDRTALGEPKNVVLDDK
SDLRLFSEWAKAGTQNSSHIWMQLNHPGKQIPKFLCANPVAPSAISLERGLEKGFNTPRALTESEIHEIITKFATSAKLA
KQAGFTGVQIHGAHGYLVSQFLSSRHNKRNDKWGGSLENRLRFVLEVYNAIRKEVGAEFPVGIKLNSADFMKGGFTEKES
MQVVQALSNNGIDLIEISGGTYESPSMMGAKDKNKPVKESTVKREAYFMDYMEKVRTLVNTPLVVTGGFRSAPAMNEALN
TSATDFIGIARTMAVDPDFPNKLMHDSNYGMPLAEPTTGKPALDKMAMVGLVWYEHQMWRIADGKDADPQLSAFSVVLKT
LLSAGWYAFKKRRA
>Mature_414_residues
MNSINLNQPFTLTNGQVIKNRLFKSAMSEQLGDKLHNPTSGLVTLYARWAKGGIGLSMTGNVMVDRTALGEPKNVVLDDK
SDLRLFSEWAKAGTQNSSHIWMQLNHPGKQIPKFLCANPVAPSAISLERGLEKGFNTPRALTESEIHEIITKFATSAKLA
KQAGFTGVQIHGAHGYLVSQFLSSRHNKRNDKWGGSLENRLRFVLEVYNAIRKEVGAEFPVGIKLNSADFMKGGFTEKES
MQVVQALSNNGIDLIEISGGTYESPSMMGAKDKNKPVKESTVKREAYFMDYMEKVRTLVNTPLVVTGGFRSAPAMNEALN
TSATDFIGIARTMAVDPDFPNKLMHDSNYGMPLAEPTTGKPALDKMAMVGLVWYEHQMWRIADGKDADPQLSAFSVVLKT
LLSAGWYAFKKRRA

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=327, Percent_Identity=29.6636085626911, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1787939, Length=262, Percent_Identity=30.9160305343511, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17564188, Length=355, Percent_Identity=37.1830985915493, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17559802, Length=357, Percent_Identity=38.0952380952381, Blast_Score=241, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI17559804, Length=356, Percent_Identity=37.0786516853933, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17565138, Length=356, Percent_Identity=36.5168539325843, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI72001454, Length=349, Percent_Identity=38.10888252149, Blast_Score=234, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17540738, Length=348, Percent_Identity=38.5057471264368, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17566914, Length=367, Percent_Identity=32.6975476839237, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17566916, Length=354, Percent_Identity=34.180790960452, Blast_Score=181, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6325086, Length=125, Percent_Identity=36.8, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=151, Percent_Identity=35.7615894039735, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 45666; Mature: 45666

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSINLNQPFTLTNGQVIKNRLFKSAMSEQLGDKLHNPTSGLVTLYARWAKGGIGLSMTG
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEC
NVMVDRTALGEPKNVVLDDKSDLRLFSEWAKAGTQNSSHIWMQLNHPGKQIPKFLCANPV
CEEEEHHCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCC
APSAISLERGLEKGFNTPRALTESEIHEIITKFATSAKLAKQAGFTGVQIHGAHGYLVSQ
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
FLSSRHNKRNDKWGGSLENRLRFVLEVYNAIRKEVGAEFPVGIKLNSADFMKGGFTEKES
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHCCCCCCHHH
MQVVQALSNNGIDLIEISGGTYESPSMMGAKDKNKPVKESTVKREAYFMDYMEKVRTLVN
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
TPLVVTGGFRSAPAMNEALNTSATDFIGIARTMAVDPDFPNKLMHDSNYGMPLAEPTTGK
CCEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCC
PALDKMAMVGLVWYEHQMWRIADGKDADPQLSAFSVVLKTLLSAGWYAFKKRRA
HHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSINLNQPFTLTNGQVIKNRLFKSAMSEQLGDKLHNPTSGLVTLYARWAKGGIGLSMTG
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEC
NVMVDRTALGEPKNVVLDDKSDLRLFSEWAKAGTQNSSHIWMQLNHPGKQIPKFLCANPV
CEEEEHHCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCC
APSAISLERGLEKGFNTPRALTESEIHEIITKFATSAKLAKQAGFTGVQIHGAHGYLVSQ
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
FLSSRHNKRNDKWGGSLENRLRFVLEVYNAIRKEVGAEFPVGIKLNSADFMKGGFTEKES
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHCCCCCCHHH
MQVVQALSNNGIDLIEISGGTYESPSMMGAKDKNKPVKESTVKREAYFMDYMEKVRTLVN
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
TPLVVTGGFRSAPAMNEALNTSATDFIGIARTMAVDPDFPNKLMHDSNYGMPLAEPTTGK
CCEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCC
PALDKMAMVGLVWYEHQMWRIADGKDADPQLSAFSVVLKTLLSAGWYAFKKRRA
HHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]