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The map label for this gene is bioF

Identifier: 172087813

GI number: 172087813

Start: 842933

End: 844084

Strand: Reverse

Name: bioF

Synonym: VF_A0747

Alternate gene names: 172087813

Gene position: 844084-842933 (Counterclockwise)

Preceding gene: 172087814

Following gene: 59713929

Centisome position: 63.45

GC content: 42.19

Gene sequence:

>1152_bases
ATGAAGCAAGCGTTTAATCAACGTATTAGCTCTGCGCTTAATCAACGTAAGCAAGCAGGGCTAAATCGCTCACGTCGAGT
GGTATCGCAAGGAAATCAGGCCACATTAGTTGTTGATGGAAAAAGCTATTTGAATTTTTCTGGAAATGATTATTTAGGCT
TGGCGAGTAGCAAAGAGTTGATGGAAGCGTGGCAAGAGGGACTATCTTTATACGGAAGTGGCAGTGGCGCCTCTCCATTA
GTCACTGGTTACTCAAAGCCGCATGCCAATTTGGAATCTCAGTTGGCAGAATGGCTTGGCCTTGATTGTGCGATTTTGTT
TAATTCAGGTTTTAGTGCCAATCAAGCGGTACTCTTTTCATTATTAGAAAAGGGTGACACCTTATTACAAGATAAATTGA
ATCATGCCTCACTAATGGAAGCGGGAATGTTATCTCCCGCAGTGATGAAACGCTTTAAACACAATGATGTTGACCACTTA
AAGAGTTTGTTAAAGCGTTCATCAGATGAGCCAACATTGGTGGTGACGGAAGGCGTATTTAGTATGGATGGGGATCTTTC
TCCATTAGCCGATATTGCAAAACTCACTAAGGAAAATGATGCTTGGCTAATGGTGGATGATGCCCATGGGTGTGGAGTGT
TAGGTACTAATGGTAAAGGCTGCTGTGATGTTTATTCGATAACGCCGGATATATTAATTGTTACTTTCGGAAAAGGGTTC
GGATTGTCTGGTGCGGCTGTGCTTTGCAATCAAGAGTGTGGTGATTATTTATCGCAATTTGCACGTCATCATGTTTATTC
TACGGCAATGCCTCCAGCTCAAGCACATGCGTTATCTCATGCCTTATTGATGATCCAACAACAAGAATGGCGACGTGACA
AACTCAAAGAATTAAATCAACAATTTGAGTCTGAATTAATGAATTTTCTGGGTGCAGAAAAAACACCAACGCCAATTAAG
CCTATTATCATAGGTGAAGCAACCGATGCGATGATACTCGCTGATTCGTTAAAAGAGCGAGGGTTATGGACAACAGCAAT
TCGTCCACCAACGGTTCCAGTCGGTTCAGCTCGAATAAGAGTGACCTTAAGTGCCAATCATTCATCAGCAGATATTTCAG
CGCTGACACAAGCGATAAACGAATTAGGATGA

Upstream 100 bases:

>100_bases
GAACAAGTAGCGCAAAAACCTGATCAAATTCAAGAGCATGAATTGCTTGATAGAGTGGCAGAACGTGTTGCAGCTCGTCC
AAATAAAGATGACTTGTTCT

Downstream 100 bases:

>100_bases
GGTGTTATGGTGAATCCAGCAGTTGCCATCGATATGTTTAATTCAACATCAAATAAAGAAAAACAAGCGATTCAAGCTGC
ATTTAGTAAAGCGGCGCATA

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKQAFNQRISSALNQRKQAGLNRSRRVVSQGNQATLVVDGKSYLNFSGNDYLGLASSKELMEAWQEGLSLYGSGSGASPL
VTGYSKPHANLESQLAEWLGLDCAILFNSGFSANQAVLFSLLEKGDTLLQDKLNHASLMEAGMLSPAVMKRFKHNDVDHL
KSLLKRSSDEPTLVVTEGVFSMDGDLSPLADIAKLTKENDAWLMVDDAHGCGVLGTNGKGCCDVYSITPDILIVTFGKGF
GLSGAAVLCNQECGDYLSQFARHHVYSTAMPPAQAHALSHALLMIQQQEWRRDKLKELNQQFESELMNFLGAEKTPTPIK
PIIIGEATDAMILADSLKERGLWTTAIRPPTVPVGSARIRVTLSANHSSADISALTQAINELG

Sequences:

>Translated_383_residues
MKQAFNQRISSALNQRKQAGLNRSRRVVSQGNQATLVVDGKSYLNFSGNDYLGLASSKELMEAWQEGLSLYGSGSGASPL
VTGYSKPHANLESQLAEWLGLDCAILFNSGFSANQAVLFSLLEKGDTLLQDKLNHASLMEAGMLSPAVMKRFKHNDVDHL
KSLLKRSSDEPTLVVTEGVFSMDGDLSPLADIAKLTKENDAWLMVDDAHGCGVLGTNGKGCCDVYSITPDILIVTFGKGF
GLSGAAVLCNQECGDYLSQFARHHVYSTAMPPAQAHALSHALLMIQQQEWRRDKLKELNQQFESELMNFLGAEKTPTPIK
PIIIGEATDAMILADSLKERGLWTTAIRPPTVPVGSARIRVTLSANHSSADISALTQAINELG
>Mature_383_residues
MKQAFNQRISSALNQRKQAGLNRSRRVVSQGNQATLVVDGKSYLNFSGNDYLGLASSKELMEAWQEGLSLYGSGSGASPL
VTGYSKPHANLESQLAEWLGLDCAILFNSGFSANQAVLFSLLEKGDTLLQDKLNHASLMEAGMLSPAVMKRFKHNDVDHL
KSLLKRSSDEPTLVVTEGVFSMDGDLSPLADIAKLTKENDAWLMVDDAHGCGVLGTNGKGCCDVYSITPDILIVTFGKGF
GLSGAAVLCNQECGDYLSQFARHHVYSTAMPPAQAHALSHALLMIQQQEWRRDKLKELNQQFESELMNFLGAEKTPTPIK
PIIIGEATDAMILADSLKERGLWTTAIRPPTVPVGSARIRVTLSANHSSADISALTQAINELG

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=377, Percent_Identity=33.1564986737401, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI284448556, Length=348, Percent_Identity=33.3333333333333, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI83977442, Length=382, Percent_Identity=31.151832460733, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI83977444, Length=382, Percent_Identity=31.151832460733, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI83977440, Length=382, Percent_Identity=31.151832460733, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI119220554, Length=365, Percent_Identity=28.7671232876712, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4758668, Length=365, Percent_Identity=28.4931506849315, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI4502025, Length=330, Percent_Identity=32.1212121212121, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI40316939, Length=330, Percent_Identity=32.1212121212121, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI5454084, Length=263, Percent_Identity=25.8555133079848, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786993, Length=356, Percent_Identity=50.561797752809, Blast_Score=350, Evalue=9e-98,
Organism=Escherichia coli, GI1790046, Length=395, Percent_Identity=32.9113924050633, Blast_Score=221, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI71994529, Length=388, Percent_Identity=33.2474226804124, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI32566772, Length=362, Percent_Identity=25.9668508287293, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17560912, Length=363, Percent_Identity=28.3746556473829, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17560914, Length=363, Percent_Identity=28.3746556473829, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71982617, Length=291, Percent_Identity=23.3676975945017, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982625, Length=291, Percent_Identity=23.3676975945017, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320438, Length=372, Percent_Identity=30.9139784946237, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320267, Length=363, Percent_Identity=26.7217630853994, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6323954, Length=219, Percent_Identity=27.8538812785388, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24662918, Length=374, Percent_Identity=33.1550802139037, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17137420, Length=346, Percent_Identity=32.0809248554913, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17136286, Length=362, Percent_Identity=27.0718232044199, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653280, Length=298, Percent_Identity=26.1744966442953, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24653276, Length=298, Percent_Identity=26.1744966442953, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24653278, Length=298, Percent_Identity=26.1744966442953, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_VIBF1 (Q5DZH9)

Other databases:

- EMBL:   CP000021
- RefSeq:   YP_206705.2
- HSSP:   P12998
- ProteinModelPortal:   Q5DZH9
- STRING:   Q5DZH9
- GeneID:   3280394
- GenomeReviews:   CP000021_GR
- KEGG:   vfi:VF_A0747
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   RLRICLH
- ProtClustDB:   PRK05958
- BioCyc:   VFIS312309:VFA0747-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 41451; Mature: 41451

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 23-23 BINDING 135-135 BINDING 181-181 BINDING 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAFNQRISSALNQRKQAGLNRSRRVVSQGNQATLVVDGKSYLNFSGNDYLGLASSKEL
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCEECCCCCCEEECCCCHHH
MEAWQEGLSLYGSGSGASPLVTGYSKPHANLESQLAEWLGLDCAILFNSGFSANQAVLFS
HHHHHHHHHEECCCCCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
LLEKGDTLLQDKLNHASLMEAGMLSPAVMKRFKHNDVDHLKSLLKRSSDEPTLVVTEGVF
HHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCC
SMDGDLSPLADIAKLTKENDAWLMVDDAHGCGVLGTNGKGCCDVYSITPDILIVTFGKGF
CCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEECCCCCCCEEEEECCCCEEEEEECCCC
GLSGAAVLCNQECGDYLSQFARHHVYSTAMPPAQAHALSHALLMIQQQEWRRDKLKELNQ
CCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFESELMNFLGAEKTPTPIKPIIIGEATDAMILADSLKERGLWTTAIRPPTVPVGSARIR
HHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEEE
VTLSANHSSADISALTQAINELG
EEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQAFNQRISSALNQRKQAGLNRSRRVVSQGNQATLVVDGKSYLNFSGNDYLGLASSKEL
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCEECCCCCCEEECCCCHHH
MEAWQEGLSLYGSGSGASPLVTGYSKPHANLESQLAEWLGLDCAILFNSGFSANQAVLFS
HHHHHHHHHEECCCCCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
LLEKGDTLLQDKLNHASLMEAGMLSPAVMKRFKHNDVDHLKSLLKRSSDEPTLVVTEGVF
HHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCC
SMDGDLSPLADIAKLTKENDAWLMVDDAHGCGVLGTNGKGCCDVYSITPDILIVTFGKGF
CCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEECCCCCCCEEEEECCCCEEEEEECCCC
GLSGAAVLCNQECGDYLSQFARHHVYSTAMPPAQAHALSHALLMIQQQEWRRDKLKELNQ
CCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFESELMNFLGAEKTPTPIKPIIIGEATDAMILADSLKERGLWTTAIRPPTVPVGSARIR
HHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEEE
VTLSANHSSADISALTQAINELG
EEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA