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The map label for this gene is gspI [H]

Identifier: 59713076

GI number: 59713076

Start: 2766013

End: 2766390

Strand: Reverse

Name: gspI [H]

Synonym: VF_2469

Alternate gene names: 59713076

Gene position: 2766390-2766013 (Counterclockwise)

Preceding gene: 59713077

Following gene: 59713075

Centisome position: 95.47

GC content: 39.68

Gene sequence:

>378_bases
ATGAGGAATAAGACATATCAGCGAACTTCTTCTAAAGGCATGACATTACTTGAAGTCTTAGTTGCGCTTGCTATTTTCGC
AACTGCAGCCCTAAGTGTGTTGCGCTCGGTGACTCAACATATAAATACATTAAGTTATTTAGAAGAAAAGACGTTTGCAA
GCATAGTGGTGGATAATCAAATGGCATTAATGATGTTAGATAAACCACCGACAACGAAGAAGAAGGGAGAGACAGAACTG
GCAGGACAAACATGGTATTGGACGATTGCTCCAGTAAAAACAACGTCTGATATTTTAAAATCTGTGGATGTATCTGTGTC
GACCACTAAAGATCAAGAATCCCCTTTAATTACGGTAAGAACGTATGTTGCGCCGTAG

Upstream 100 bases:

>100_bases
TCTCCGCAAGGTGCTCGTGGAAACCTTGATGATAAATCATGGCTTATTCGCGTTGAAGAATCCGGAGTGATTAAATTATT
TGCTCCGGGGGAGGAACGTG

Downstream 100 bases:

>100_bases
TTTTTCTCAACCTTTTTCAAAAGCAAGAGGCTTCACACTCATTGAAGTGATGGTTGCTATTGCTGTATTTGCGACCTTGA
GCTTTGCTGCGTATCAAGTA

Product: general secretion pathway protein I

Products: NA

Alternate protein names: Cholera toxin secretion protein epsI [H]

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MRNKTYQRTSSKGMTLLEVLVALAIFATAALSVLRSVTQHINTLSYLEEKTFASIVVDNQMALMMLDKPPTTKKKGETEL
AGQTWYWTIAPVKTTSDILKSVDVSVSTTKDQESPLITVRTYVAP

Sequences:

>Translated_125_residues
MRNKTYQRTSSKGMTLLEVLVALAIFATAALSVLRSVTQHINTLSYLEEKTFASIVVDNQMALMMLDKPPTTKKKGETEL
AGQTWYWTIAPVKTTSDILKSVDVSVSTTKDQESPLITVRTYVAP
>Mature_125_residues
MRNKTYQRTSSKGMTLLEVLVALAIFATAALSVLRSVTQHINTLSYLEEKTFASIVVDNQMALMMLDKPPTTKKKGETEL
AGQTWYWTIAPVKTTSDILKSVDVSVSTTKDQESPLITVRTYVAP

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2165

COG function: function code NU; Type II secretory pathway, pseudopilin PulG

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP I family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010052
- InterPro:   IPR012902
- InterPro:   IPR001120
- InterPro:   IPR003413 [H]

Pfam domain/function: PF02501 GSPII_IJ; PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 13855; Mature: 13855

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKTYQRTSSKGMTLLEVLVALAIFATAALSVLRSVTQHINTLSYLEEKTFASIVVDNQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
MALMMLDKPPTTKKKGETELAGQTWYWTIAPVKTTSDILKSVDVSVSTTKDQESPLITVR
EEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCEECCCCCCCCEEEEE
TYVAP
EEECC
>Mature Secondary Structure
MRNKTYQRTSSKGMTLLEVLVALAIFATAALSVLRSVTQHINTLSYLEEKTFASIVVDNQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
MALMMLDKPPTTKKKGETELAGQTWYWTIAPVKTTSDILKSVDVSVSTTKDQESPLITVR
EEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCEECCCCCCCCEEEEE
TYVAP
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]