Click here to switch to the map view.

The map label for this gene is gspJ [H]

Identifier: 59713075

GI number: 59713075

Start: 2765346

End: 2766026

Strand: Reverse

Name: gspJ [H]

Synonym: VF_2468

Alternate gene names: 59713075

Gene position: 2766026-2765346 (Counterclockwise)

Preceding gene: 59713076

Following gene: 59713074

Centisome position: 95.46

GC content: 37.89

Gene sequence:

>681_bases
ATGTTGCGCCGTAGTTTTTCTCAACCTTTTTCAAAAGCAAGAGGCTTCACACTCATTGAAGTGATGGTTGCTATTGCTGT
ATTTGCGACCTTGAGCTTTGCTGCGTATCAAGTAGTAAACCAAGTACAACGCAGTAATGAACAATCATTAGAAAAAAGTG
AGCGAATTAAACAGCTGCAACGTAGTTTGGTTTTTCTCGATAATGATTTTCGACAAATTGTTGCTCGCCAATTTCGAACG
GGTGGTGAAAAAGCCTCGGAAGCCTTGTTGAAATCGGGAGAGTATTTCCTTGATTCTGAATTCCAAGCCATTTTGTTTGT
GCGTCTTGGTTGGAAAAACCCACAACAAGCCTTTCCTCGTGGTGAAATTACTAAAGTTGGTTATCGAGTTATTGAAAATA
AATTAGAGCGATTATGGTGGCGATATCCAGATACGCCTGTTGGTCAGGAAGCGGTAATTACTCCGCTTATTGATGGCATT
GAAGAGCTAAAATTTGAATTTTATGATGGTAAAAAATGGCTAAAAAAATGGGAAATGAAAAATCAATTACCCAATGCCAT
TAAAATGATACTCACGTTTAAAGATTATGGTGACGTTGAGCGTGTTTACCTTACTCCAGGATTTAAACTGGATAATAATG
CTAATCAAAATAGTGATGATTCAAAAGAGGATCGTGGCTAA

Upstream 100 bases:

>100_bases
ATTGCTCCAGTAAAAACAACGTCTGATATTTTAAAATCTGTGGATGTATCTGTGTCGACCACTAAAGATCAAGAATCCCC
TTTAATTACGGTAAGAACGT

Downstream 100 bases:

>100_bases
TGAGAAAGGTAGGCGTGATTTCCCATTTTAAAAAGAATAAAGGCGTTGCATTGATTGTGGTACTGCTGCTTTTAGCCATT
ATGACCACCATTGCTGCCCA

Product: general secretion pathway protein J

Products: NA

Alternate protein names: Cholera toxin secretion protein epsJ [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MLRRSFSQPFSKARGFTLIEVMVAIAVFATLSFAAYQVVNQVQRSNEQSLEKSERIKQLQRSLVFLDNDFRQIVARQFRT
GGEKASEALLKSGEYFLDSEFQAILFVRLGWKNPQQAFPRGEITKVGYRVIENKLERLWWRYPDTPVGQEAVITPLIDGI
EELKFEFYDGKKWLKKWEMKNQLPNAIKMILTFKDYGDVERVYLTPGFKLDNNANQNSDDSKEDRG

Sequences:

>Translated_226_residues
MLRRSFSQPFSKARGFTLIEVMVAIAVFATLSFAAYQVVNQVQRSNEQSLEKSERIKQLQRSLVFLDNDFRQIVARQFRT
GGEKASEALLKSGEYFLDSEFQAILFVRLGWKNPQQAFPRGEITKVGYRVIENKLERLWWRYPDTPVGQEAVITPLIDGI
EELKFEFYDGKKWLKKWEMKNQLPNAIKMILTFKDYGDVERVYLTPGFKLDNNANQNSDDSKEDRG
>Mature_226_residues
MLRRSFSQPFSKARGFTLIEVMVAIAVFATLSFAAYQVVNQVQRSNEQSLEKSERIKQLQRSLVFLDNDFRQIVARQFRT
GGEKASEALLKSGEYFLDSEFQAILFVRLGWKNPQQAFPRGEITKVGYRVIENKLERLWWRYPDTPVGQEAVITPLIDGI
EELKFEFYDGKKWLKKWEMKNQLPNAIKMILTFKDYGDVERVYLTPGFKLDNNANQNSDDSKEDRG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG4795

COG function: function code U; Type II secretory pathway, component PulJ

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP J family [H]

Homologues:

Organism=Escherichia coli, GI1789728, Length=203, Percent_Identity=25.1231527093596, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010055
- InterPro:   IPR012902
- InterPro:   IPR001120 [H]

Pfam domain/function: PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 26335; Mature: 26335

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRSFSQPFSKARGFTLIEVMVAIAVFATLSFAAYQVVNQVQRSNEQSLEKSERIKQLQ
CCCCHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RSLVFLDNDFRQIVARQFRTGGEKASEALLKSGEYFLDSEFQAILFVRLGWKNPQQAFPR
HHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHCCCCHHEEEEEEECCCCHHHHCCC
GEITKVGYRVIENKLERLWWRYPDTPVGQEAVITPLIDGIEELKFEFYDGKKWLKKWEMK
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
NQLPNAIKMILTFKDYGDVERVYLTPGFKLDNNANQNSDDSKEDRG
HHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRRSFSQPFSKARGFTLIEVMVAIAVFATLSFAAYQVVNQVQRSNEQSLEKSERIKQLQ
CCCCHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RSLVFLDNDFRQIVARQFRTGGEKASEALLKSGEYFLDSEFQAILFVRLGWKNPQQAFPR
HHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHCCCCHHEEEEEEECCCCHHHHCCC
GEITKVGYRVIENKLERLWWRYPDTPVGQEAVITPLIDGIEELKFEFYDGKKWLKKWEMK
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
NQLPNAIKMILTFKDYGDVERVYLTPGFKLDNNANQNSDDSKEDRG
HHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]