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The map label for this gene is gspK [H]

Identifier: 59713074

GI number: 59713074

Start: 2764312

End: 2765346

Strand: Reverse

Name: gspK [H]

Synonym: VF_2467

Alternate gene names: 59713074

Gene position: 2765346-2764312 (Counterclockwise)

Preceding gene: 59713075

Following gene: 59713073

Centisome position: 95.44

GC content: 39.52

Gene sequence:

>1035_bases
ATGAGAAAGGTAGGCGTGATTTCCCATTTTAAAAAGAATAAAGGCGTTGCATTGATTGTGGTACTGCTGCTTTTAGCCAT
TATGACCACCATTGCTGCCCAGATGTCAGAACGCTTATTTCTTCAGTTTCATCGTGCTCAGAATCAGTTAAATCACCAAC
AAGCGTATTGGTATTCTATTGGTGTTGAAGCCTTAGCAAAAACCGGAATAGAAGAAGCTTACAAAGATGGAAAAAACATT
AATTTAAGCCAAGCATGGGCAACTGAGGAAACCACTTATCCACTTGATTATGGTGAAGCTTATGGTGGTGTAAAAGACAA
ACAAGCTTGCTTTAACTTAAATGCACTAAGCAGTATTGAAGCCAGTAATGATTCATCTAAACGCCCTTTTTTAGTGGGAG
TATGGATGAATTTATTAGAGTCAGTGGAGGTGGATAATTACCTTGCTGAAACCATTGCTGACTCAACGTGGGAATACCTC
GACCCTGATGATGTTGTACGCTCAATGACTGGTGTTGAAGACAACACTTATCAATCGTTTAAACCAGCGTATTTAGCACC
AAATAGTTGGATGGGTGATGTCAGTGAGTTACGTGCTGTTAATGGTGTTACAGCAGAAGTTGTTGAAAAAGTATCTCCCC
TGATTTGTGCGATACCTAGTGGCAATTGGTACTTAAATGTAAACACCTTAGCGCCGGAAAATGCCAAGATATTAGTTGCG
CTTTTTTCTCCAAATTTAAGTGAAGGTGACGCAAGAAGTGTATTAGAAGATCGACCTTTTGATGGATGGGATTCGGTGGA
TGATTTTCTTGCTGAAGGACCAATTAGTCGAGTTGAAGAAAAAGTTCGTAAACAAGCAAAAGATTATTTATCCGTAGATA
GTCAGTTTTTTGAGTTAGATGCTCAAGTATTAGTAGAAGATTCGCGCGTACGTGTGCGTTCTTTATTACATAGCAGTGAT
AAGAAAGTGGTGAACGTTATCCGTCGCCAATATGGAGGAATGAGTGAGCGAACATCTGATAATACGGTTGAATAG

Upstream 100 bases:

>100_bases
AAGATTATGGTGACGTTGAGCGTGTTTACCTTACTCCAGGATTTAAACTGGATAATAATGCTAATCAAAATAGTGATGAT
TCAAAAGAGGATCGTGGCTA

Downstream 100 bases:

>100_bases
TCAGCCATCAGATCCCATTCAATGGATAGTCTGGTCTCCAGAAAATAAAGAAGTGATCGCTTCGGGTGAATTAGATTCAT
CTGAAGCATTACCTTCGTTG

Product: general secretion pathway protein K

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRKVGVISHFKKNKGVALIVVLLLLAIMTTIAAQMSERLFLQFHRAQNQLNHQQAYWYSIGVEALAKTGIEEAYKDGKNI
NLSQAWATEETTYPLDYGEAYGGVKDKQACFNLNALSSIEASNDSSKRPFLVGVWMNLLESVEVDNYLAETIADSTWEYL
DPDDVVRSMTGVEDNTYQSFKPAYLAPNSWMGDVSELRAVNGVTAEVVEKVSPLICAIPSGNWYLNVNTLAPENAKILVA
LFSPNLSEGDARSVLEDRPFDGWDSVDDFLAEGPISRVEEKVRKQAKDYLSVDSQFFELDAQVLVEDSRVRVRSLLHSSD
KKVVNVIRRQYGGMSERTSDNTVE

Sequences:

>Translated_344_residues
MRKVGVISHFKKNKGVALIVVLLLLAIMTTIAAQMSERLFLQFHRAQNQLNHQQAYWYSIGVEALAKTGIEEAYKDGKNI
NLSQAWATEETTYPLDYGEAYGGVKDKQACFNLNALSSIEASNDSSKRPFLVGVWMNLLESVEVDNYLAETIADSTWEYL
DPDDVVRSMTGVEDNTYQSFKPAYLAPNSWMGDVSELRAVNGVTAEVVEKVSPLICAIPSGNWYLNVNTLAPENAKILVA
LFSPNLSEGDARSVLEDRPFDGWDSVDDFLAEGPISRVEEKVRKQAKDYLSVDSQFFELDAQVLVEDSRVRVRSLLHSSD
KKVVNVIRRQYGGMSERTSDNTVE
>Mature_344_residues
MRKVGVISHFKKNKGVALIVVLLLLAIMTTIAAQMSERLFLQFHRAQNQLNHQQAYWYSIGVEALAKTGIEEAYKDGKNI
NLSQAWATEETTYPLDYGEAYGGVKDKQACFNLNALSSIEASNDSSKRPFLVGVWMNLLESVEVDNYLAETIADSTWEYL
DPDDVVRSMTGVEDNTYQSFKPAYLAPNSWMGDVSELRAVNGVTAEVVEKVSPLICAIPSGNWYLNVNTLAPENAKILVA
LFSPNLSEGDARSVLEDRPFDGWDSVDDFLAEGPISRVEEKVRKQAKDYLSVDSQFFELDAQVLVEDSRVRVRSLLHSSD
KKVVNVIRRQYGGMSERTSDNTVE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=275, Percent_Identity=31.6363636363636, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 38466; Mature: 38466

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVGVISHFKKNKGVALIVVLLLLAIMTTIAAQMSERLFLQFHRAQNQLNHQQAYWYSI
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVEALAKTGIEEAYKDGKNINLSQAWATEETTYPLDYGEAYGGVKDKQACFNLNALSSIE
HHHHHHHHCHHHHHHCCCCCCCHHHCCCCCCCCCCCCHHHHCCCCCHHHHHCCHHHHHCC
ASNDSSKRPFLVGVWMNLLESVEVDNYLAETIADSTWEYLDPDDVVRSMTGVEDNTYQSF
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHCC
KPAYLAPNSWMGDVSELRAVNGVTAEVVEKVSPLICAIPSGNWYLNVNTLAPENAKILVA
CCCEECCCCCCCCHHHHHHHCCCHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEEEE
LFSPNLSEGDARSVLEDRPFDGWDSVDDFLAEGPISRVEEKVRKQAKDYLSVDSQFFELD
EECCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVLVEDSRVRVRSLLHSSDKKVVNVIRRQYGGMSERTSDNTVE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRKVGVISHFKKNKGVALIVVLLLLAIMTTIAAQMSERLFLQFHRAQNQLNHQQAYWYSI
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVEALAKTGIEEAYKDGKNINLSQAWATEETTYPLDYGEAYGGVKDKQACFNLNALSSIE
HHHHHHHHCHHHHHHCCCCCCCHHHCCCCCCCCCCCCHHHHCCCCCHHHHHCCHHHHHCC
ASNDSSKRPFLVGVWMNLLESVEVDNYLAETIADSTWEYLDPDDVVRSMTGVEDNTYQSF
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHCC
KPAYLAPNSWMGDVSELRAVNGVTAEVVEKVSPLICAIPSGNWYLNVNTLAPENAKILVA
CCCEECCCCCCCCHHHHHHHCCCHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEEEE
LFSPNLSEGDARSVLEDRPFDGWDSVDDFLAEGPISRVEEKVRKQAKDYLSVDSQFFELD
EECCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVLVEDSRVRVRSLLHSSDKKVVNVIRRQYGGMSERTSDNTVE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]