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The map label for this gene is plsB [H]

Identifier: 59713050

GI number: 59713050

Start: 2743670

End: 2746093

Strand: Direct

Name: plsB [H]

Synonym: VF_2443

Alternate gene names: 59713050

Gene position: 2743670-2746093 (Clockwise)

Preceding gene: 59713046

Following gene: 59713053

Centisome position: 94.69

GC content: 38.86

Gene sequence:

>2424_bases
ATGTCTACAGGACACACTATTTATCATTCTTTACTAAAGTTGCCACTATCGGTAATGGTGAAGAGTAGCTCGATTCCATC
GAATCCTATTGAAGATTTAAAAATTGATCTTGAGCGCCCAATTATTTACGCACTACCATTTCGTTCACATGTGGATTTAC
TTACACTACAAAAAAGTGCCCTAGAACTTGGTTTGCCTGACCCTCTATCTCCTATCGAGATTGAAGGTGTAAAATATCCA
CGCTATGTATTTACTTCTATCGGACCAAAAATGTTCGATACTGATGACGATCTTCCACAAGAGTCGTTAGACCTATTTAA
AATTGTATTAAAGCACCATGCTGATAATCCAGACGCTGATTTCCAACTGATCCCTACCTCTATTTTATGGGGAAGAAAGC
CAGGTAAAGAAGGAACAAGTAAACCTCATTTAATGCCACTTAACGGACCTCAAAAATTTGTCACGTTAATAAAAGCAGGA
CGAGACTCAACCGTTCGTATTAGCCCAGTAGTATCTTTACGTTACATGGCTGATAACCACGGCTCTGATGAAGCCATTGC
ACATAAATTAGCGCGTGTTGCAAAAATACACTTTTCTCGCCAAAAATTAGCGGCATCAGGTCCTAACCTACCGAATCGCC
AAGCGCTATTTAATCGCTTATTAAAATCACAAGCGATTGAGAAAGTCATTTTAGAAGAAGCAAAAAGCCGAAATGTCGAT
GTAGAGAAAGTGCGTAAAGAAGCCATGGGTATCATGGAAGAAATCGCTACCAACTTCTCTTATTCTCTAATTAAAAATGG
CAACCGTATTTTAAAATGGTTATGGAACCGTTTATATCAGGGATTAAACATCAATAACGCTTCTACTGTGCGTAAATTAG
CACAAGAAGGTCATGAGATTGTTTATGTTCCTTGTCATCGTAGTCACATGGATTACCTACTCCTTTCTTATGTTTTATAC
CATGAAGGTTTAGTTCCACCACATATTGCTGCAGGCATTAACTTAAACTTCTTCCCTGCGGGTCCTATTTTCCGTCGCGG
TGGTGCTTTCTTTATTCGTCGTAGCTTTAAAGGTAATCGCCTGTACTCAACCATCTTCCGTGAATATTTAGCTGAGCTTT
TTGCAAAAGGATACTCAGTTGAATACTTCAGTGAAGGTGGACGTTCACGTACTGGTCGTTTATTACAAGCGAAAACAGGT
ATGTTAGCAATGACGGTACAAGCAATGCTACGTGGCTTAAACCGCCCAGTTACCTTAGTACCTGTTTATATTGGTTATGA
ACACGTAATGGAAGTAACCACTTACGCAAAAGAGCTACGTGGTAAACGTAAAGAAAAAGAAAATGCAGGTCAGGTATTAC
GTACACTACGCAAACTACGTAATTTCGGACAAGGTTACGTTAACTTTGGTGAGCCTATCTCATTAAACCATTACTTAAAT
GAGCATGCTCCAAACTGGTCTGAATCAATCAACCCGATTGAACCACAGAAACCAGAATGGATGACACCTGTCGTAAACGG
CATCGCTAATAAGATGATGACACATATTAATGATGCTGCTGCAGCTAACGCATTAACATTATGTGCAACCGCTTTACTTG
CTGCAAACCAACGAGCACTAAGTAAAGAAGACTTAACAGAACAATTAGATTGTTACCTGCAAATTCTACGTAATGTTCCA
TACTCAGCAACGGCAACTGTTCCAAGTGAAGATGCTGACGCTTTATTAGAACATGCCATTAAGCTGGATAAGTTTGTTAT
TGAGAAAGATACGCTAGGTGAGATTGTTTCATTAGATCGTAACCAGTCTCTACTGATGACTTACTATCGAAATAACATTA
TCCATCTGTTTGCACTACCATCATTGATTGCAAAACTCGTCGTTCACCATGACACGATTACCGTAGAACAAATTCAGGAC
CAGATAAAATTAATTTATCCTTTCCTAAAAGCGGAATTGTTCTTACATTACAAAGAGGAAGAATTGACCTCTATAGTAAA
TAATCATATTGATGAATTAGTTCAACAAAATCTGATTTTACGTGATGGTGATACACTGCAACTGTGTAATGCAAACATTC
GCAAATTGCATTTACTTGCTCATACCATTTCAGAAACTCTACAGCGCTATGCAATCGCACTAACGCATCTTCAAGCATCA
CCAGATTTGGGCAAAGATGAATTAGAAGAGCAAAGCCAAATCATGGCTCAGCGTTTAAGTCGTCTACATGGTATCAACGC
ACCTGAGTTCTTTGATAAAGGTGTATTCTGCATTCTGTTTAATACTCTAAAAACAGAAGGATACCTTGATGAAGACGGCG
CAGCAGTGTTAAGTAAAGTTGAGCCGTTATCGCAAGATATCGCACACCTTCTTACTCCTGAAATTAAGCTAACGATTCAT
GCAGTAATGACAAAAGAAGACTAA

Upstream 100 bases:

>100_bases
CTATTAAAAGGTTTGACCAGTTCTGCTATTCTTGCCTGAAATTGTAAGCATCTATTATACGGCCGACTGCTCGGTACATA
ACTAAGGCGTTATTGTCATT

Downstream 100 bases:

>100_bases
TTTAACGTCCCTTTCTTTAAATACAAAAAAAGCCAGTATGATATTAGTCATACTGGCTTTTCTTTTAAAACAACACGTTA
AATTAAAACGCTTGCACTGA

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MSTGHTIYHSLLKLPLSVMVKSSSIPSNPIEDLKIDLERPIIYALPFRSHVDLLTLQKSALELGLPDPLSPIEIEGVKYP
RYVFTSIGPKMFDTDDDLPQESLDLFKIVLKHHADNPDADFQLIPTSILWGRKPGKEGTSKPHLMPLNGPQKFVTLIKAG
RDSTVRISPVVSLRYMADNHGSDEAIAHKLARVAKIHFSRQKLAASGPNLPNRQALFNRLLKSQAIEKVILEEAKSRNVD
VEKVRKEAMGIMEEIATNFSYSLIKNGNRILKWLWNRLYQGLNINNASTVRKLAQEGHEIVYVPCHRSHMDYLLLSYVLY
HEGLVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTG
MLAMTVQAMLRGLNRPVTLVPVYIGYEHVMEVTTYAKELRGKRKEKENAGQVLRTLRKLRNFGQGYVNFGEPISLNHYLN
EHAPNWSESINPIEPQKPEWMTPVVNGIANKMMTHINDAAAANALTLCATALLAANQRALSKEDLTEQLDCYLQILRNVP
YSATATVPSEDADALLEHAIKLDKFVIEKDTLGEIVSLDRNQSLLMTYYRNNIIHLFALPSLIAKLVVHHDTITVEQIQD
QIKLIYPFLKAELFLHYKEEELTSIVNNHIDELVQQNLILRDGDTLQLCNANIRKLHLLAHTISETLQRYAIALTHLQAS
PDLGKDELEEQSQIMAQRLSRLHGINAPEFFDKGVFCILFNTLKTEGYLDEDGAAVLSKVEPLSQDIAHLLTPEIKLTIH
AVMTKED

Sequences:

>Translated_807_residues
MSTGHTIYHSLLKLPLSVMVKSSSIPSNPIEDLKIDLERPIIYALPFRSHVDLLTLQKSALELGLPDPLSPIEIEGVKYP
RYVFTSIGPKMFDTDDDLPQESLDLFKIVLKHHADNPDADFQLIPTSILWGRKPGKEGTSKPHLMPLNGPQKFVTLIKAG
RDSTVRISPVVSLRYMADNHGSDEAIAHKLARVAKIHFSRQKLAASGPNLPNRQALFNRLLKSQAIEKVILEEAKSRNVD
VEKVRKEAMGIMEEIATNFSYSLIKNGNRILKWLWNRLYQGLNINNASTVRKLAQEGHEIVYVPCHRSHMDYLLLSYVLY
HEGLVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTG
MLAMTVQAMLRGLNRPVTLVPVYIGYEHVMEVTTYAKELRGKRKEKENAGQVLRTLRKLRNFGQGYVNFGEPISLNHYLN
EHAPNWSESINPIEPQKPEWMTPVVNGIANKMMTHINDAAAANALTLCATALLAANQRALSKEDLTEQLDCYLQILRNVP
YSATATVPSEDADALLEHAIKLDKFVIEKDTLGEIVSLDRNQSLLMTYYRNNIIHLFALPSLIAKLVVHHDTITVEQIQD
QIKLIYPFLKAELFLHYKEEELTSIVNNHIDELVQQNLILRDGDTLQLCNANIRKLHLLAHTISETLQRYAIALTHLQAS
PDLGKDELEEQSQIMAQRLSRLHGINAPEFFDKGVFCILFNTLKTEGYLDEDGAAVLSKVEPLSQDIAHLLTPEIKLTIH
AVMTKED
>Mature_806_residues
STGHTIYHSLLKLPLSVMVKSSSIPSNPIEDLKIDLERPIIYALPFRSHVDLLTLQKSALELGLPDPLSPIEIEGVKYPR
YVFTSIGPKMFDTDDDLPQESLDLFKIVLKHHADNPDADFQLIPTSILWGRKPGKEGTSKPHLMPLNGPQKFVTLIKAGR
DSTVRISPVVSLRYMADNHGSDEAIAHKLARVAKIHFSRQKLAASGPNLPNRQALFNRLLKSQAIEKVILEEAKSRNVDV
EKVRKEAMGIMEEIATNFSYSLIKNGNRILKWLWNRLYQGLNINNASTVRKLAQEGHEIVYVPCHRSHMDYLLLSYVLYH
EGLVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTGM
LAMTVQAMLRGLNRPVTLVPVYIGYEHVMEVTTYAKELRGKRKEKENAGQVLRTLRKLRNFGQGYVNFGEPISLNHYLNE
HAPNWSESINPIEPQKPEWMTPVVNGIANKMMTHINDAAAANALTLCATALLAANQRALSKEDLTEQLDCYLQILRNVPY
SATATVPSEDADALLEHAIKLDKFVIEKDTLGEIVSLDRNQSLLMTYYRNNIIHLFALPSLIAKLVVHHDTITVEQIQDQ
IKLIYPFLKAELFLHYKEEELTSIVNNHIDELVQQNLILRDGDTLQLCNANIRKLHLLAHTISETLQRYAIALTHLQASP
DLGKDELEEQSQIMAQRLSRLHGINAPEFFDKGVFCILFNTLKTEGYLDEDGAAVLSKVEPLSQDIAHLLTPEIKLTIHA
VMTKED

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=428, Percent_Identity=30.8411214953271, Blast_Score=166, Evalue=8e-41,
Organism=Homo sapiens, GI190358539, Length=430, Percent_Identity=24.6511627906977, Blast_Score=121, Evalue=3e-27,
Organism=Escherichia coli, GI87082362, Length=807, Percent_Identity=57.6208178438662, Blast_Score=951, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=267, Percent_Identity=35.2059925093633, Blast_Score=151, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71988723, Length=498, Percent_Identity=27.9116465863454, Blast_Score=141, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71988728, Length=416, Percent_Identity=28.8461538461538, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI17864692, Length=273, Percent_Identity=33.3333333333333, Blast_Score=162, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24650754, Length=480, Percent_Identity=26.25, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24650752, Length=480, Percent_Identity=26.25, Blast_Score=128, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21357731, Length=480, Percent_Identity=26.25, Blast_Score=128, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 91139; Mature: 91008

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGHTIYHSLLKLPLSVMVKSSSIPSNPIEDLKIDLERPIIYALPFRSHVDLLTLQKSA
CCCCHHHHHHHHHCCHHHHEECCCCCCCCHHHHHEECCCCEEEEECCCCCCHHHHHHHHH
LELGLPDPLSPIEIEGVKYPRYVFTSIGPKMFDTDDDLPQESLDLFKIVLKHHADNPDAD
HHCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
FQLIPTSILWGRKPGKEGTSKPHLMPLNGPQKFVTLIKAGRDSTVRISPVVSLRYMADNH
EEEECHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEEEEEEEECCCC
GSDEAIAHKLARVAKIHFSRQKLAASGPNLPNRQALFNRLLKSQAIEKVILEEAKSRNVD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEKVRKEAMGIMEEIATNFSYSLIKNGNRILKWLWNRLYQGLNINNASTVRKLAQEGHEI
HHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEE
VYVPCHRSHMDYLLLSYVLYHEGLVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNR
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCEEEEEECCCCCH
LYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTGMLAMTVQAMLRGLNRPVTLV
HHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEE
PVYIGYEHVMEVTTYAKELRGKRKEKENAGQVLRTLRKLRNFGQGYVNFGEPISLNHYLN
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
EHAPNWSESINPIEPQKPEWMTPVVNGIANKMMTHINDAAAANALTLCATALLAANQRAL
HCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SKEDLTEQLDCYLQILRNVPYSATATVPSEDADALLEHAIKLDKFVIEKDTLGEIVSLDR
CHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
NQSLLMTYYRNNIIHLFALPSLIAKLVVHHDTITVEQIQDQIKLIYPFLKAELFLHYKEE
CCHHHHEEHHCCEEEEHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHEECHH
ELTSIVNNHIDELVQQNLILRDGDTLQLCNANIRKLHLLAHTISETLQRYAIALTHLQAS
HHHHHHHHHHHHHHHCCCEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDLGKDELEEQSQIMAQRLSRLHGINAPEFFDKGVFCILFNTLKTEGYLDEDGAAVLSKV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEHHHCCCCCCCCCHHHHHHHH
EPLSQDIAHLLTPEIKLTIHAVMTKED
HHHHHHHHHHHCCCCEEEEEEEEECCC
>Mature Secondary Structure 
STGHTIYHSLLKLPLSVMVKSSSIPSNPIEDLKIDLERPIIYALPFRSHVDLLTLQKSA
CCCHHHHHHHHHCCHHHHEECCCCCCCCHHHHHEECCCCEEEEECCCCCCHHHHHHHHH
LELGLPDPLSPIEIEGVKYPRYVFTSIGPKMFDTDDDLPQESLDLFKIVLKHHADNPDAD
HHCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
FQLIPTSILWGRKPGKEGTSKPHLMPLNGPQKFVTLIKAGRDSTVRISPVVSLRYMADNH
EEEECHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEEEEEEEECCCC
GSDEAIAHKLARVAKIHFSRQKLAASGPNLPNRQALFNRLLKSQAIEKVILEEAKSRNVD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEKVRKEAMGIMEEIATNFSYSLIKNGNRILKWLWNRLYQGLNINNASTVRKLAQEGHEI
HHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEE
VYVPCHRSHMDYLLLSYVLYHEGLVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNR
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCEEEEEECCCCCH
LYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTGMLAMTVQAMLRGLNRPVTLV
HHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEE
PVYIGYEHVMEVTTYAKELRGKRKEKENAGQVLRTLRKLRNFGQGYVNFGEPISLNHYLN
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
EHAPNWSESINPIEPQKPEWMTPVVNGIANKMMTHINDAAAANALTLCATALLAANQRAL
HCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SKEDLTEQLDCYLQILRNVPYSATATVPSEDADALLEHAIKLDKFVIEKDTLGEIVSLDR
CHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
NQSLLMTYYRNNIIHLFALPSLIAKLVVHHDTITVEQIQDQIKLIYPFLKAELFLHYKEE
CCHHHHEEHHCCEEEEHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHEECHH
ELTSIVNNHIDELVQQNLILRDGDTLQLCNANIRKLHLLAHTISETLQRYAIALTHLQAS
HHHHHHHHHHHHHHHCCCEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDLGKDELEEQSQIMAQRLSRLHGINAPEFFDKGVFCILFNTLKTEGYLDEDGAAVLSKV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEHHHCCCCCCCCCHHHHHHHH
EPLSQDIAHLLTPEIKLTIHAVMTKED
HHHHHHHHHHHCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA