Click here to switch to the map view.

The map label for this gene is lexA

Identifier: 59713049

GI number: 59713049

Start: 2742802

End: 2743428

Strand: Reverse

Name: lexA

Synonym: VF_2442

Alternate gene names: 59713049

Gene position: 2743428-2742802 (Counterclockwise)

Preceding gene: 59713051

Following gene: 59713048

Centisome position: 94.68

GC content: 42.42

Gene sequence:

>627_bases
ATGAAGCCGCTAACGGCAAGACAGCAAGAAGTCTTTGATCTTATCAAAGCAAAAATTGATGATACTGGCATGCCACCAAC
ACGTGCAGAAATTGCTCGTGAGTTGGGATTTCGTTCAGCGAATGCGGCGGAAGAGCACTTAAAGGCATTAGCTCGTAAGC
AGGTGATAGAAATTATTCCTGGTGCATCTCGTGGAATACGTATTCTACTGCAAGATGCAGATCATCATGATGAAGAACTG
GGTGTTCCACTCATTGGTCAAGTTGCAGCAGGGGAGCCGATTCTTGCTCAAGAGCATGTTGAGAGCCACTATAAAGTTGA
TCCTGGAATGTTTAAGCCACAAGCTGATTTCTTATTACGCGTGAACGGCGAAAGCATGAAAGACATTGGAATTATGGATG
GTGATTTACTTGCGGTTCATAAAACTCAAGACGTTCGCGATGGTCAAGTCGTTGTTGCTCGTGTTGATGATGATGTAACG
GTAAAACGCTTAGAGCGTAAAGGCTCTATGGTATTTCTTCATGCCGAAAATGAAGAATTTTCACCAATTGAAGTGGATTT
AACTTCTCAAAGTTTAAGTATTGAAGGTCTTGCTGTTGGTGTGATCAGAAGTACAACTTGGATGTAA

Upstream 100 bases:

>100_bases
AAAAGTAAGCGATTTTGTCTTATTTTTAATCAATAGGTCTTTCAAAGCATAGCACAGCTGTATATACTCACAGTGAACTG
TATAAACAGACAGGTGATTT

Downstream 100 bases:

>100_bases
TTACAATCTAATCATCTAGATTGACACTATATAATCATCATTATCGCTTGTTTAGCTGAAATAAATCTTTACAAGTGGTA
ATGATAGTTATTATCATTTA

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKPLTARQQEVFDLIKAKIDDTGMPPTRAEIARELGFRSANAAEEHLKALARKQVIEIIPGASRGIRILLQDADHHDEEL
GVPLIGQVAAGEPILAQEHVESHYKVDPGMFKPQADFLLRVNGESMKDIGIMDGDLLAVHKTQDVRDGQVVVARVDDDVT
VKRLERKGSMVFLHAENEEFSPIEVDLTSQSLSIEGLAVGVIRSTTWM

Sequences:

>Translated_208_residues
MKPLTARQQEVFDLIKAKIDDTGMPPTRAEIARELGFRSANAAEEHLKALARKQVIEIIPGASRGIRILLQDADHHDEEL
GVPLIGQVAAGEPILAQEHVESHYKVDPGMFKPQADFLLRVNGESMKDIGIMDGDLLAVHKTQDVRDGQVVVARVDDDVT
VKRLERKGSMVFLHAENEEFSPIEVDLTSQSLSIEGLAVGVIRSTTWM
>Mature_208_residues
MKPLTARQQEVFDLIKAKIDDTGMPPTRAEIARELGFRSANAAEEHLKALARKQVIEIIPGASRGIRILLQDADHHDEEL
GVPLIGQVAAGEPILAQEHVESHYKVDPGMFKPQADFLLRVNGESMKDIGIMDGDLLAVHKTQDVRDGQVVVARVDDDVT
VKRLERKGSMVFLHAENEEFSPIEVDLTSQSLSIEGLAVGVIRSTTWM

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=208, Percent_Identity=72.5961538461538, Blast_Score=303, Evalue=4e-84,
Organism=Escherichia coli, GI1787431, Length=129, Percent_Identity=31.7829457364341, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_VIBF1 (Q5E209)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205825.1
- ProteinModelPortal:   Q5E209
- SMR:   Q5E209
- STRING:   Q5E209
- MEROPS:   S24.001
- GeneID:   3279659
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2442
- NMPDR:   fig|312309.3.peg.2442
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- PhylomeDB:   Q5E209
- ProtClustDB:   PRK00215
- BioCyc:   VFIS312309:VF2442-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22965; Mature: 22965

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: ACT_SITE 125-125 ACT_SITE 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLTARQQEVFDLIKAKIDDTGMPPTRAEIARELGFRSANAAEEHLKALARKQVIEIIP
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
GASRGIRILLQDADHHDEELGVPLIGQVAAGEPILAQEHVESHYKVDPGMFKPQADFLLR
CCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHEECCCCCCCCCCCEEEE
VNGESMKDIGIMDGDLLAVHKTQDVRDGQVVVARVDDDVTVKRLERKGSMVFLHAENEEF
ECCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHCCCCEEEEEECCCCC
SPIEVDLTSQSLSIEGLAVGVIRSTTWM
CCEEEEECCCCEEECEEEEEEECCCCCC
>Mature Secondary Structure
MKPLTARQQEVFDLIKAKIDDTGMPPTRAEIARELGFRSANAAEEHLKALARKQVIEIIP
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
GASRGIRILLQDADHHDEELGVPLIGQVAAGEPILAQEHVESHYKVDPGMFKPQADFLLR
CCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHEECCCCCCCCCCCEEEE
VNGESMKDIGIMDGDLLAVHKTQDVRDGQVVVARVDDDVTVKRLERKGSMVFLHAENEEF
ECCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHCCCCEEEEEECCCCC
SPIEVDLTSQSLSIEGLAVGVIRSTTWM
CCEEEEECCCCEEECEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA