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The map label for this gene is trmA [H]

Identifier: 59713043

GI number: 59713043

Start: 2736443

End: 2737549

Strand: Direct

Name: trmA [H]

Synonym: VF_2436

Alternate gene names: 59713043

Gene position: 2736443-2737549 (Clockwise)

Preceding gene: 59713039

Following gene: 59713046

Centisome position: 94.44

GC content: 36.59

Gene sequence:

>1107_bases
ATGACACAATCAGTCATGAACCCAGAAAATTATCAAGTACAGTTAGATGAAAAAGCAGAAGCACTCTCTGCTATGTTTTC
TGAATTTAATGTTCCAGAACTAGAAGTCTTTTCCTCTCCTGCTGAAAACTACCGCATGCGTGCTGAATTTCGTGTGTGGC
ATGAAGGTGACGAAATGTATTACGTCATGTTCAACCAAGAAACCAAAGAAAAATACCGTGTTGATTACTTCTTACCTGCA
AGCCGTTTAATTAACGATTTGATGCCATTACTAACAGAAGCAGTAAAAGAGAGTAAAACACTTCGCTATAAAATGTTCCA
AGTGGATTTCTTATCAACCCTTAGTGGCGAAATTTTGGTGTCGATGCTTTATCACCGTCAACTAGATGATGCTTGGAAAG
AAGAAGCGAAAGCATTAAAACAACGTCTGAATGATGAAGGTTTTAACCTAAATATCATTGGTCGTGCACGTAAAATGAAA
ATCGTTTTAGACCAAGAGTTTGTTATTGAAAAACTTAAAGTTAATGACGACATCTTAACGTATAAGCAAGTTGAGAATAG
CTTTACACAACCAAACGGCATTGTTGCTCAAAAAATGCTGGAATGGGCAGTGGATTGTACACAAAACAGTCAAGGCGACC
TACTTGAATTATATTGTGGTAACGGTAACTTCTCATTGGCATTAGCGAAAAACTTTGATCGTGTTTTAGCAACCGAATTA
GCTAAACCATCTGTTGATTCTGCTCAATACAACATTGCAGCAAATAACATTGATAATGTACAAATCATTCGAATGTCAGC
TGAAGATTTTACTGATGCTATGGAAGGTAAACGTGAATTTAGACGCTTAAAAGATCAAAACATCGATTTAAAAAGCTATA
ACTGCAACACTATCTTTGTTGATCCACCTCGTTCTGGAATGGATGAAGGGACTTGTAAGATGGTTCAAGGTTACGAGCGT
ATTATGTACATCTCTTGTAATCCTGAAACGCTAAAAGAAAACTTAGAAATTTTAAGCCAAACACACAACATTACGCGTTT
TGCTTTATTTGACCAGTTCCCATACACACACCATATGGAAGCAGGCGTTTTCTTAGAAAGAAAATAA

Upstream 100 bases:

>100_bases
TAGTATTAATTAGTTTCGATCAATAACACACAAATACTGGACAACAGATCTCTATCGCATAAAATCGGCGCTCTTATTTT
GAGCAGCGTGAGGCAAGACG

Downstream 100 bases:

>100_bases
ACTTAAAAAAAAGGCTCAGAGCGTAATGTTCTGAGCCTTTTTTATGAACGGTTATTTATATTTCTGTGTGAGTATCTTTC
TTTTTCAAAATACCCATTTT

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTQSVMNPENYQVQLDEKAEALSAMFSEFNVPELEVFSSPAENYRMRAEFRVWHEGDEMYYVMFNQETKEKYRVDYFLPA
SRLINDLMPLLTEAVKESKTLRYKMFQVDFLSTLSGEILVSMLYHRQLDDAWKEEAKALKQRLNDEGFNLNIIGRARKMK
IVLDQEFVIEKLKVNDDILTYKQVENSFTQPNGIVAQKMLEWAVDCTQNSQGDLLELYCGNGNFSLALAKNFDRVLATEL
AKPSVDSAQYNIAANNIDNVQIIRMSAEDFTDAMEGKREFRRLKDQNIDLKSYNCNTIFVDPPRSGMDEGTCKMVQGYER
IMYISCNPETLKENLEILSQTHNITRFALFDQFPYTHHMEAGVFLERK

Sequences:

>Translated_368_residues
MTQSVMNPENYQVQLDEKAEALSAMFSEFNVPELEVFSSPAENYRMRAEFRVWHEGDEMYYVMFNQETKEKYRVDYFLPA
SRLINDLMPLLTEAVKESKTLRYKMFQVDFLSTLSGEILVSMLYHRQLDDAWKEEAKALKQRLNDEGFNLNIIGRARKMK
IVLDQEFVIEKLKVNDDILTYKQVENSFTQPNGIVAQKMLEWAVDCTQNSQGDLLELYCGNGNFSLALAKNFDRVLATEL
AKPSVDSAQYNIAANNIDNVQIIRMSAEDFTDAMEGKREFRRLKDQNIDLKSYNCNTIFVDPPRSGMDEGTCKMVQGYER
IMYISCNPETLKENLEILSQTHNITRFALFDQFPYTHHMEAGVFLERK
>Mature_367_residues
TQSVMNPENYQVQLDEKAEALSAMFSEFNVPELEVFSSPAENYRMRAEFRVWHEGDEMYYVMFNQETKEKYRVDYFLPAS
RLINDLMPLLTEAVKESKTLRYKMFQVDFLSTLSGEILVSMLYHRQLDDAWKEEAKALKQRLNDEGFNLNIIGRARKMKI
VLDQEFVIEKLKVNDDILTYKQVENSFTQPNGIVAQKMLEWAVDCTQNSQGDLLELYCGNGNFSLALAKNFDRVLATELA
KPSVDSAQYNIAANNIDNVQIIRMSAEDFTDAMEGKREFRRLKDQNIDLKSYNCNTIFVDPPRSGMDEGTCKMVQGYERI
MYISCNPETLKENLEILSQTHNITRFALFDQFPYTHHMEAGVFLERK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=220, Percent_Identity=26.8181818181818, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI51173878, Length=220, Percent_Identity=26.8181818181818, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1790403, Length=368, Percent_Identity=61.1413043478261, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1789148, Length=286, Percent_Identity=26.9230769230769, Blast_Score=85, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24666579, Length=422, Percent_Identity=23.696682464455, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 42807; Mature: 42676

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSVMNPENYQVQLDEKAEALSAMFSEFNVPELEVFSSPAENYRMRAEFRVWHEGDEMY
CCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEECCCHHHCEEEEEEEEEECCCEEE
YVMFNQETKEKYRVDYFLPASRLINDLMPLLTEAVKESKTLRYKMFQVDFLSTLSGEILV
EEEECCCCCHHEEEEEECCHHHHHHHHHHHHHHHHHHCCHHEEEEEEEHHHHHHHHHHHH
SMLYHRQLDDAWKEEAKALKQRLNDEGFNLNIIGRARKMKIVLDQEFVIEKLKVNDDILT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECHHHHHHHHCCCCCHHH
YKQVENSFTQPNGIVAQKMLEWAVDCTQNSQGDLLELYCGNGNFSLALAKNFDRVLATEL
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHH
AKPSVDSAQYNIAANNIDNVQIIRMSAEDFTDAMEGKREFRRLKDQNIDLKSYNCNTIFV
CCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEE
DPPRSGMDEGTCKMVQGYERIMYISCNPETLKENLEILSQTHNITRFALFDQFPYTHHME
CCCCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECC
AGVFLERK
CCEEEECC
>Mature Secondary Structure 
TQSVMNPENYQVQLDEKAEALSAMFSEFNVPELEVFSSPAENYRMRAEFRVWHEGDEMY
CCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEECCCHHHCEEEEEEEEEECCCEEE
YVMFNQETKEKYRVDYFLPASRLINDLMPLLTEAVKESKTLRYKMFQVDFLSTLSGEILV
EEEECCCCCHHEEEEEECCHHHHHHHHHHHHHHHHHHCCHHEEEEEEEHHHHHHHHHHHH
SMLYHRQLDDAWKEEAKALKQRLNDEGFNLNIIGRARKMKIVLDQEFVIEKLKVNDDILT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECHHHHHHHHCCCCCHHH
YKQVENSFTQPNGIVAQKMLEWAVDCTQNSQGDLLELYCGNGNFSLALAKNFDRVLATEL
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHH
AKPSVDSAQYNIAANNIDNVQIIRMSAEDFTDAMEGKREFRRLKDQNIDLKSYNCNTIFV
CCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEE
DPPRSGMDEGTCKMVQGYERIMYISCNPETLKENLEILSQTHNITRFALFDQFPYTHHME
CCCCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECC
AGVFLERK
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA