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The map label for this gene is murB

Identifier: 59713033

GI number: 59713033

Start: 2711622

End: 2712671

Strand: Reverse

Name: murB

Synonym: VF_2426

Alternate gene names: 59713033

Gene position: 2712671-2711622 (Counterclockwise)

Preceding gene: 59713040

Following gene: 59713032

Centisome position: 93.62

GC content: 34.0

Gene sequence:

>1050_bases
ATGCAGATTTTACTTAATAAAACACTTAAGCCTTATAATAGTTTTTCAGTTAACGAAAGTGCTGATTTAATCATTCAAGC
TGATTCTATACAGGATCTAATTGATATTTGGTCTGATAAAAAATACACAGACATGACAAAACTACCTTTAGGGCGAGGGA
GTAATACCTTATTTTGTAATCACTTTAATGGTGTCGTTGTTCTTAATCGACTTTTCGGAAAATCAGTAACAGAAACGGAT
ACTGATTATTTGCTGAAAATTTCTAGTGGAGAAGATTGGCCTGCATTAGTTGAGTGGTGTGTTGATAATGGATTCGCCGG
CATTGAGAATCTAGCAATGATTCCAGGTTGTGCAGGTTCTGCTCCTATTCAAAATATCGGTGCTTATGGTCTTGAGCTAA
AAGATATATGTGAGTCTGTTGAATATTTGGATCTTGAAACACTTCAAATTAAAACATTGAAGAATTCAGAATGTTTATTT
GGTTATCGAGAATCTATTTTTAAGCACGAGCTCAAAGATCGTTGCATCATTACAGCGATAACACTTCGTTTAAATAAACA
ATGGCAGCCTGTTCTTGCTTATGGCCCATTGAGTGATTTACGAAATAGTAAAACCACACCTAAGAACGTATTTGATAAAA
TTTGTGAAATTCGAAGTAAAAAACTCCCTGATCCAAATGTGATTGGAAATGCAGGTAGTTTCTTTAAGAATCCTGTTATA
TCAGAAGAGCACTATTTAGCCTTATGTGAAACTTACCCTAATTTACCTGCTTATGATGTCACTGAAGGGAAGAAAATAGC
TGCTGGTTGGCTTATTGATAATGCTGGTTTAAAAGGTTTCAAAATTAATGGAGCCCAAGTACATCAAGAACAAGCGTTAG
TGCTAATTAACACTGGTACAGCGACATCTGAAGATATTTTAGAGTTAGCAAATCATGTAAAAAATAGTGTCTTAGATATG
TATGATATTGAACTTGAACATGAAGTTAGATTTTATCTAAATGGTGAAGAATCTTTTTTATCGGAGCTCTTTGATGAAAG
AACACACTAA

Upstream 100 bases:

>100_bases
TTGTCTAGATGAGAAACGGTAAGATTCATTAGCTCTGTTCTATATAGTTGTTATGTATTAAGTTAGAATGTACTTAATCT
AATCAATTAAATACAGCACT

Downstream 100 bases:

>100_bases
AAAACTTGAGATCTTAAGACTGCTTAGTGATGGACACTTTCATTCAGGTGAAGAGTTGGGTGAACAACTTAATATCTCTC
GAGCCGCAATAAGCAAACAC

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MQILLNKTLKPYNSFSVNESADLIIQADSIQDLIDIWSDKKYTDMTKLPLGRGSNTLFCNHFNGVVVLNRLFGKSVTETD
TDYLLKISSGEDWPALVEWCVDNGFAGIENLAMIPGCAGSAPIQNIGAYGLELKDICESVEYLDLETLQIKTLKNSECLF
GYRESIFKHELKDRCIITAITLRLNKQWQPVLAYGPLSDLRNSKTTPKNVFDKICEIRSKKLPDPNVIGNAGSFFKNPVI
SEEHYLALCETYPNLPAYDVTEGKKIAAGWLIDNAGLKGFKINGAQVHQEQALVLINTGTATSEDILELANHVKNSVLDM
YDIELEHEVRFYLNGEESFLSELFDERTH

Sequences:

>Translated_349_residues
MQILLNKTLKPYNSFSVNESADLIIQADSIQDLIDIWSDKKYTDMTKLPLGRGSNTLFCNHFNGVVVLNRLFGKSVTETD
TDYLLKISSGEDWPALVEWCVDNGFAGIENLAMIPGCAGSAPIQNIGAYGLELKDICESVEYLDLETLQIKTLKNSECLF
GYRESIFKHELKDRCIITAITLRLNKQWQPVLAYGPLSDLRNSKTTPKNVFDKICEIRSKKLPDPNVIGNAGSFFKNPVI
SEEHYLALCETYPNLPAYDVTEGKKIAAGWLIDNAGLKGFKINGAQVHQEQALVLINTGTATSEDILELANHVKNSVLDM
YDIELEHEVRFYLNGEESFLSELFDERTH
>Mature_349_residues
MQILLNKTLKPYNSFSVNESADLIIQADSIQDLIDIWSDKKYTDMTKLPLGRGSNTLFCNHFNGVVVLNRLFGKSVTETD
TDYLLKISSGEDWPALVEWCVDNGFAGIENLAMIPGCAGSAPIQNIGAYGLELKDICESVEYLDLETLQIKTLKNSECLF
GYRESIFKHELKDRCIITAITLRLNKQWQPVLAYGPLSDLRNSKTTPKNVFDKICEIRSKKLPDPNVIGNAGSFFKNPVI
SEEHYLALCETYPNLPAYDVTEGKKIAAGWLIDNAGLKGFKINGAQVHQEQALVLINTGTATSEDILELANHVKNSVLDM
YDIELEHEVRFYLNGEESFLSELFDERTH

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=328, Percent_Identity=47.8658536585366, Blast_Score=328, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_VIBF1 (Q5E225)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205809.1
- ProteinModelPortal:   Q5E225
- SMR:   Q5E225
- STRING:   Q5E225
- GeneID:   3277745
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2426
- NMPDR:   fig|312309.3.peg.2426
- eggNOG:   COG0812
- HOGENOM:   HBG686573
- OMA:   NIVEITA
- PhylomeDB:   Q5E225
- ProtClustDB:   PRK00046
- BioCyc:   VFIS312309:VF2426-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 39166; Mature: 39166

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 163-163 ACT_SITE 233-233 ACT_SITE 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILLNKTLKPYNSFSVNESADLIIQADSIQDLIDIWSDKKYTDMTKLPLGRGSNTLFCN
CEEEECCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEE
HFNGVVVLNRLFGKSVTETDTDYLLKISSGEDWPALVEWCVDNGFAGIENLAMIPGCAGS
CCCCEEEHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHEECCCCCCC
APIQNIGAYGLELKDICESVEYLDLETLQIKTLKNSECLFGYRESIFKHELKDRCIITAI
CCHHHHCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEE
TLRLNKQWQPVLAYGPLSDLRNSKTTPKNVFDKICEIRSKKLPDPNVIGNAGSFFKNPVI
EEEECCCCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
SEEHYLALCETYPNLPAYDVTEGKKIAAGWLIDNAGLKGFKINGAQVHQEQALVLINTGT
CCCCEEEEEHHCCCCCCEECCCCCEEEEEEEEECCCCCEEEECCCEECCCCEEEEEECCC
ATSEDILELANHVKNSVLDMYDIELEHEVRFYLNGEESFLSELFDERTH
CCHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQILLNKTLKPYNSFSVNESADLIIQADSIQDLIDIWSDKKYTDMTKLPLGRGSNTLFCN
CEEEECCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEE
HFNGVVVLNRLFGKSVTETDTDYLLKISSGEDWPALVEWCVDNGFAGIENLAMIPGCAGS
CCCCEEEHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHEECCCCCCC
APIQNIGAYGLELKDICESVEYLDLETLQIKTLKNSECLFGYRESIFKHELKDRCIITAI
CCHHHHCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEE
TLRLNKQWQPVLAYGPLSDLRNSKTTPKNVFDKICEIRSKKLPDPNVIGNAGSFFKNPVI
EEEECCCCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
SEEHYLALCETYPNLPAYDVTEGKKIAAGWLIDNAGLKGFKINGAQVHQEQALVLINTGT
CCCCEEEEEHHCCCCCCEECCCCCEEEEEEEEECCCCCEEEECCCEECCCCEEEEEECCC
ATSEDILELANHVKNSVLDMYDIELEHEVRFYLNGEESFLSELFDERTH
CCHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA