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The map label for this gene is murI

Identifier: 59713040

GI number: 59713040

Start: 2732609

End: 2733397

Strand: Reverse

Name: murI

Synonym: VF_2433

Alternate gene names: 59713040

Gene position: 2733397-2732609 (Counterclockwise)

Preceding gene: 59713041

Following gene: 59713033

Centisome position: 94.34

GC content: 35.49

Gene sequence:

>789_bases
ATGCCAAAACACATTTTGATTTTTGATTCAGGTATCGGTGGATTATCGGTATATAAAGAAATTAAATATCAGTTACCTTT
AGCTAAGTATATTTATGCGTTTGATAATGCGGCATTCCCTTATGGTGAACTAACTGAATCTGTACTTATAGAAAGAACGA
CACACATTATCTCTAAGTTATGTTCTAAATTTCCTATCGATATCGTTGTGATTGCTTGTAATACAGCAAGTACTGTTGTC
CTTCCTTCTCTTAGGGAGAAATTAGATATACCTGTAGTAGGTGTTGTTCCTGCTATCAAACCCGCAGCTCTTGTATCAAA
TAAGATCGGTTTACTTGCAACGCCTGCGACAGTAAAGAGAAGCTACACGTATGATTTGATCAAATCTTTTGCTCCTATAT
CAGATGTTCAACTATTAGGCTCAACCCGACTGGTTGAAATGGCGGAAGAAAAAATGATCGGAATTGATGTAGATATTACG
GAACTAAAAGAGATTTTATCTCCTTGGCAAAATAAAGTAGATACGATAGTCCTAGGTTGTACTCATTTCCCTTTTCTAAA
GAATGAAATAAAAAAAGCACTGGGAAATAAAATATTGCTTATTGATTCTGGAGAGGCAATAGCTAGAAGAGTAAAACAAT
TATTGAATGGGGATGAGGTGGAAAGTGCAGTTCTTTTTGAAGGTGAGGTATTTTGTAGTGCCCCCTCTATAAAAGAAGAG
GCACTAAATCATACATTCAAAGAACTAAATTTTAGTTCTCTTCAGTGTTTGGGTTACCCGAAGTTTTAG

Upstream 100 bases:

>100_bases
ATCTTCGTTATAACGCTTCTGTTTTACAAAACTAGTGCAAGAGGTATGATTAGAGCTCATCTTTTTATATCGGCTTACAA
TAGAGTCTGTGGTTTCAGTT

Downstream 100 bases:

>100_bases
GATCATTAGCAATACGCACTTTTAGAGTTCGCTGATCAAATTCGTTATTGTTTAAATTTTCAATAACGTTGTCAGCGTCT
TTTGTTGCAACTACTACGAA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPKHILIFDSGIGGLSVYKEIKYQLPLAKYIYAFDNAAFPYGELTESVLIERTTHIISKLCSKFPIDIVVIACNTASTVV
LPSLREKLDIPVVGVVPAIKPAALVSNKIGLLATPATVKRSYTYDLIKSFAPISDVQLLGSTRLVEMAEEKMIGIDVDIT
ELKEILSPWQNKVDTIVLGCTHFPFLKNEIKKALGNKILLIDSGEAIARRVKQLLNGDEVESAVLFEGEVFCSAPSIKEE
ALNHTFKELNFSSLQCLGYPKF

Sequences:

>Translated_262_residues
MPKHILIFDSGIGGLSVYKEIKYQLPLAKYIYAFDNAAFPYGELTESVLIERTTHIISKLCSKFPIDIVVIACNTASTVV
LPSLREKLDIPVVGVVPAIKPAALVSNKIGLLATPATVKRSYTYDLIKSFAPISDVQLLGSTRLVEMAEEKMIGIDVDIT
ELKEILSPWQNKVDTIVLGCTHFPFLKNEIKKALGNKILLIDSGEAIARRVKQLLNGDEVESAVLFEGEVFCSAPSIKEE
ALNHTFKELNFSSLQCLGYPKF
>Mature_261_residues
PKHILIFDSGIGGLSVYKEIKYQLPLAKYIYAFDNAAFPYGELTESVLIERTTHIISKLCSKFPIDIVVIACNTASTVVL
PSLREKLDIPVVGVVPAIKPAALVSNKIGLLATPATVKRSYTYDLIKSFAPISDVQLLGSTRLVEMAEEKMIGIDVDITE
LKEILSPWQNKVDTIVLGCTHFPFLKNEIKKALGNKILLIDSGEAIARRVKQLLNGDEVESAVLFEGEVFCSAPSIKEEA
LNHTFKELNFSSLQCLGYPKF

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=259, Percent_Identity=50.1930501930502, Blast_Score=240, Evalue=7e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_VIBF1 (Q5E218)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205816.1
- HSSP:   P56868
- ProteinModelPortal:   Q5E218
- SMR:   Q5E218
- STRING:   Q5E218
- GeneID:   3279645
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2433
- NMPDR:   fig|312309.3.peg.2433
- eggNOG:   COG0796
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- PhylomeDB:   Q5E218
- ProtClustDB:   PRK00865
- BioCyc:   VFIS312309:VF2433-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 28887; Mature: 28756

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKHILIFDSGIGGLSVYKEIKYQLPLAKYIYAFDNAAFPYGELTESVLIERTTHIISKL
CCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
CSKFPIDIVVIACNTASTVVLPSLREKLDIPVVGVVPAIKPAALVSNKIGLLATPATVKR
HHHCCCEEEEEEECCCCEEECHHHHHHCCCCEEECCCCCCHHHHHHCCCCEEECCHHHHH
SYTYDLIKSFAPISDVQLLGSTRLVEMAEEKMIGIDVDITELKEILSPWQNKVDTIVLGC
HHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCEEEEEC
THFPFLKNEIKKALGNKILLIDSGEAIARRVKQLLNGDEVESAVLFEGEVFCSAPSIKEE
CCCHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEECCEEEECCCCHHHH
ALNHTFKELNFSSLQCLGYPKF
HHHHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure 
PKHILIFDSGIGGLSVYKEIKYQLPLAKYIYAFDNAAFPYGELTESVLIERTTHIISKL
CCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
CSKFPIDIVVIACNTASTVVLPSLREKLDIPVVGVVPAIKPAALVSNKIGLLATPATVKR
HHHCCCEEEEEEECCCCEEECHHHHHHCCCCEEECCCCCCHHHHHHCCCCEEECCHHHHH
SYTYDLIKSFAPISDVQLLGSTRLVEMAEEKMIGIDVDITELKEILSPWQNKVDTIVLGC
HHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCEEEEEC
THFPFLKNEIKKALGNKILLIDSGEAIARRVKQLLNGDEVESAVLFEGEVFCSAPSIKEE
CCCHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEECCEEEECCCCHHHH
ALNHTFKELNFSSLQCLGYPKF
HHHHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA