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The map label for this gene is birA [H]

Identifier: 59713032

GI number: 59713032

Start: 2710682

End: 2711638

Strand: Reverse

Name: birA [H]

Synonym: VF_2425

Alternate gene names: 59713032

Gene position: 2711638-2710682 (Counterclockwise)

Preceding gene: 59713033

Following gene: 59713030

Centisome position: 93.58

GC content: 38.45

Gene sequence:

>957_bases
ATGAAAGAACACACTAAAAAACTTGAGATCTTAAGACTGCTTAGTGATGGACACTTTCATTCAGGTGAAGAGTTGGGTGA
ACAACTTAATATCTCTCGAGCCGCAATAAGCAAACACATTAATGTGATTCAGAGTTGGGGACTTGAGATTTACAAAGTAA
AAGGTAAAGGTTACTCACTCTCAACTCCTATTGAGTTATTACAACAAGAATTAGTTTCTCATAATAAGCTACCGAAACCT
GAAGTTCTTGGCGTTGTTGATTCAACAAATCAGTATTTGCTAAATAATATGACCTCTCTTGAGTGCGGACAATCTTGTAT
AGCTGAATATCAATCTGCTGGGCGAGGCCGAAGAGGGCGCTCATGGGTATCTCCATTTGGTGGAAATATCTATTTGTCTC
TCTATTGGAGATTAGATGATGGTCTTGCTGCTACGATGGGATTAAGTTTAGCTGTTGGTATTGCTGTTGTTGAAGCCCTG
GAAAACTTAGGCTGTAGTGGTCTTAAATTAAAGTGGCCTAATGATATTTATTGGAATAACCGAAAGCTAGGCGGTGTTCT
GATTGAACTTTCGGCTCAATCAGGTGGTGCCGCCCATGTGGTCATAGGTATCGGAATTAATGTAGACCTTAGTGAAAGAT
TTACTTCTGAAATAGACCAACCTTGGGTTGATTTAAAAACGATTCTTGGCCAAAACCACATTAGAAATAAATTAGCGAAT
ACATTATTGGTTTCGCTTGAAGAAACGATGAAAGCGTTTGAGTGTTTTGGTCTATCCCACTTTATTGAAAGATGGAATAG
ATATGACAATTTTAAAGGCCAAGAAATTTCGCTTCAATTAGGAAATGGTACCGTCACAGGAGTTTGCAACGGTATTGATG
CTCAAGGTGCACTGCTACTTATCAATGCTGATGGCCTAACGGCTTATAATGGTGGTGAAATATCTATAAAGAAATAA

Upstream 100 bases:

>100_bases
CATGTAAAAAATAGTGTCTTAGATATGTATGATATTGAACTTGAACATGAAGTTAGATTTTATCTAAATGGTGAAGAATC
TTTTTTATCGGAGCTCTTTG

Downstream 100 bases:

>100_bases
TAGCTACTTTCTTATTGCTACTTTCTTATTGCTACTTGGTTAATTGAATGGTTTTCACCTTTTGAAAGAATAAGACTAGC
TCGATTCTTTGTTGGTAAGA

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKEHTKKLEILRLLSDGHFHSGEELGEQLNISRAAISKHINVIQSWGLEIYKVKGKGYSLSTPIELLQQELVSHNKLPKP
EVLGVVDSTNQYLLNNMTSLECGQSCIAEYQSAGRGRRGRSWVSPFGGNIYLSLYWRLDDGLAATMGLSLAVGIAVVEAL
ENLGCSGLKLKWPNDIYWNNRKLGGVLIELSAQSGGAAHVVIGIGINVDLSERFTSEIDQPWVDLKTILGQNHIRNKLAN
TLLVSLEETMKAFECFGLSHFIERWNRYDNFKGQEISLQLGNGTVTGVCNGIDAQGALLLINADGLTAYNGGEISIKK

Sequences:

>Translated_318_residues
MKEHTKKLEILRLLSDGHFHSGEELGEQLNISRAAISKHINVIQSWGLEIYKVKGKGYSLSTPIELLQQELVSHNKLPKP
EVLGVVDSTNQYLLNNMTSLECGQSCIAEYQSAGRGRRGRSWVSPFGGNIYLSLYWRLDDGLAATMGLSLAVGIAVVEAL
ENLGCSGLKLKWPNDIYWNNRKLGGVLIELSAQSGGAAHVVIGIGINVDLSERFTSEIDQPWVDLKTILGQNHIRNKLAN
TLLVSLEETMKAFECFGLSHFIERWNRYDNFKGQEISLQLGNGTVTGVCNGIDAQGALLLINADGLTAYNGGEISIKK
>Mature_318_residues
MKEHTKKLEILRLLSDGHFHSGEELGEQLNISRAAISKHINVIQSWGLEIYKVKGKGYSLSTPIELLQQELVSHNKLPKP
EVLGVVDSTNQYLLNNMTSLECGQSCIAEYQSAGRGRRGRSWVSPFGGNIYLSLYWRLDDGLAATMGLSLAVGIAVVEAL
ENLGCSGLKLKWPNDIYWNNRKLGGVLIELSAQSGGAAHVVIGIGINVDLSERFTSEIDQPWVDLKTILGQNHIRNKLAN
TLLVSLEETMKAFECFGLSHFIERWNRYDNFKGQEISLQLGNGTVTGVCNGIDAQGALLLINADGLTAYNGGEISIKK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=252, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790408, Length=318, Percent_Identity=46.8553459119497, Blast_Score=307, Evalue=7e-85,
Organism=Drosophila melanogaster, GI24644246, Length=134, Percent_Identity=37.3134328358209, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 34923; Mature: 34923

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEHTKKLEILRLLSDGHFHSGEELGEQLNISRAAISKHINVIQSWGLEIYKVKGKGYSL
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
STPIELLQQELVSHNKLPKPEVLGVVDSTNQYLLNNMTSLECGQSCIAEYQSAGRGRRGR
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
SWVSPFGGNIYLSLYWRLDDGLAATMGLSLAVGIAVVEALENLGCSGLKLKWPNDIYWNN
CCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEECC
RKLGGVLIELSAQSGGAAHVVIGIGINVDLSERFTSEIDQPWVDLKTILGQNHIRNKLAN
CEECEEEEEEECCCCCEEEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHH
TLLVSLEETMKAFECFGLSHFIERWNRYDNFKGQEISLQLGNGTVTGVCNGIDAQGALLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCCCEEEE
INADGLTAYNGGEISIKK
EECCCEEEECCCEEEEEC
>Mature Secondary Structure
MKEHTKKLEILRLLSDGHFHSGEELGEQLNISRAAISKHINVIQSWGLEIYKVKGKGYSL
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
STPIELLQQELVSHNKLPKPEVLGVVDSTNQYLLNNMTSLECGQSCIAEYQSAGRGRRGR
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
SWVSPFGGNIYLSLYWRLDDGLAATMGLSLAVGIAVVEALENLGCSGLKLKWPNDIYWNN
CCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEECC
RKLGGVLIELSAQSGGAAHVVIGIGINVDLSERFTSEIDQPWVDLKTILGQNHIRNKLAN
CEECEEEEEEECCCCCEEEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHH
TLLVSLEETMKAFECFGLSHFIERWNRYDNFKGQEISLQLGNGTVTGVCNGIDAQGALLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCCCEEEE
INADGLTAYNGGEISIKK
EECCCEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]