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The map label for this gene is tufA [H]

Identifier: 59713030

GI number: 59713030

Start: 2707874

End: 2709058

Strand: Reverse

Name: tufA [H]

Synonym: VF_2423

Alternate gene names: 59713030

Gene position: 2709058-2707874 (Counterclockwise)

Preceding gene: 59713032

Following gene: 172087776

Centisome position: 93.5

GC content: 45.57

Gene sequence:

>1185_bases
ATGTCTAAAGAAAAATTTGAACGTACGAAACCGCACGTAAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACAAC
TCTAACAGCTGCTATCTGTACTACACTTGCAAAAATCTACGGCGGTGTAGCTAAAGATTTCGCATCAATCGATAACGCTC
CTGAAGAGCGCGAGCGTGGTATCACAATCTCAACTTCTCACGTTGAGTACGATACTCCTGCACGTCACTACGCACACGTT
GACTGTCCTGGACACGCCGATTATGTTAAAAATATGATCACTGGTGCTGCTCAAATGGATGGTGGTATCCTAGTTGTTGC
TGCAACAGATGGTCCAATGCCACAAACTCGTGAGCACATCCTACTTGGTCGTCAAGTTGGTATCCCTTACATCATCGTAT
TCATGAACAAATGTGACATGGTTGATGATGAAGAGCTTCTAGAACTAGTTGAAATGGAAGTTCGTGAACTTCTTTCTGAG
TACGACTTCCCAGGTGATGATCTACCAGTAATTCAAGGTTCAGCTCTTGGCGCTCTAAACGGCGAAAAAGAGTGGGAAGA
CAAGATCGTTGAGCTTGCAGAAGCACTAGATTCTTACATCCCAGAACCAGAGCGTGCTGTTGACCAACCGTTCCTACTAC
CAATTGAAGATGTATTCTCAATCCAAGGTCGTGGTACAGTTGTAACTGGTCGTATCGAGCGTGGTATCCTACGCGTAGGT
GACGAAGTAGAAATCGTTGGTATCAAAGAAACAACAATGACTACTTGTACAGGTGTTGAAATGTTCCGTAAACTGCTTGA
CGAAGGTCGTGCAGGTGAGAACGTTGGTGCACTACTACGTGGTACTAAGCGTGATGACGTTGAACGTGGCCAAGTACTTG
CTGCTAAAGGTTCAATCAACCCACACACTAAATTTGAGTCAGAAGTGTACGTTCTTTCTAAAGATGAAGGCGGCCGTCAC
ACTCCTTTCTTCAAAGGTTACCGTCCACAGTTCTACTTCCGTACAACTGACGTAACAGGCGACATCACTCTACCAGAAGG
CGTAGAAATGGTAATGCCAGGTGATAACGTTCAAATGACGGTTGAGCTAATCGCTCCAATCGCAATGGACGAAGGTCTAC
GCTTCGCGATCCGTGAAGGTGGCCGTACAGTTGGTGCTGGTGTTGTTGCTAAAATCTTTGCTTAA

Upstream 100 bases:

>100_bases
CAGATTGCCTATTTCTCCTTTTAAAAAATCATATATTCAATCTATATTTGGTTACGTGGTCATTCAAAGCCACTAATATC
CGTACCTAGAGGGACTATCC

Downstream 100 bases:

>100_bases
GATTTGACTAAGTACTAGTAAAAAGGGCATCATTTGATGCCCTTTTTCTGCGTTGTATTGTGTGTTGTTTTGCATTTTTA
GAAAATACACAGCACAGCGC

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKIYGGVAKDFASIDNAPEERERGITISTSHVEYDTPARHYAHV
DCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALGALNGEKEWEDKIVELAEALDSYIPEPERAVDQPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
DEVEIVGIKETTMTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLAAKGSINPHTKFESEVYVLSKDEGGRH
TPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA

Sequences:

>Translated_394_residues
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKIYGGVAKDFASIDNAPEERERGITISTSHVEYDTPARHYAHV
DCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALGALNGEKEWEDKIVELAEALDSYIPEPERAVDQPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
DEVEIVGIKETTMTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLAAKGSINPHTKFESEVYVLSKDEGGRH
TPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
>Mature_393_residues
SKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKIYGGVAKDFASIDNAPEERERGITISTSHVEYDTPARHYAHVD
CPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEY
DFPGDDLPVIQGSALGALNGEKEWEDKIVELAEALDSYIPEPERAVDQPFLLPIEDVFSIQGRGTVVTGRIERGILRVGD
EVEIVGIKETTMTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLAAKGSINPHTKFESEVYVLSKDEGGRHT
PFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=55.9493670886076, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=30, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=30, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI4503475, Length=353, Percent_Identity=32.8611898016997, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=28.47533632287, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI54607086, Length=343, Percent_Identity=29.1545189504373, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI194018522, Length=325, Percent_Identity=30.1538461538462, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI194018520, Length=330, Percent_Identity=29.6969696969697, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI194097354, Length=330, Percent_Identity=29.6969696969697, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=29.8136645962733, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI4503507, Length=277, Percent_Identity=27.7978339350181, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI53729339, Length=229, Percent_Identity=31.8777292576419, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI53729337, Length=229, Percent_Identity=31.8777292576419, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157426893, Length=301, Percent_Identity=26.578073089701, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI82546879, Length=339, Percent_Identity=24.4837758112094, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=85.0253807106599, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=85.2417302798982, Blast_Score=669, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=379, Percent_Identity=29.287598944591, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1789108, Length=335, Percent_Identity=27.4626865671642, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994988, Length=249, Percent_Identity=29.3172690763052, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788922, Length=339, Percent_Identity=26.5486725663717, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=50.3759398496241, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.9612403100775, Blast_Score=327, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=331, Percent_Identity=32.02416918429, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17569207, Length=331, Percent_Identity=32.02416918429, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=430, Percent_Identity=27.906976744186, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=430, Percent_Identity=27.906976744186, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566303, Length=313, Percent_Identity=30.3514376996805, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=242, Percent_Identity=30.9917355371901, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32566629, Length=353, Percent_Identity=27.1954674220963, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17509919, Length=441, Percent_Identity=27.2108843537415, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566301, Length=158, Percent_Identity=33.5443037974684, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=255, Percent_Identity=27.0588235294118, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=62.2784810126582, Blast_Score=505, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=28.8636363636364, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=28.8636363636364, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=360, Percent_Identity=25, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=30.0291545189504, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=365, Percent_Identity=26.3013698630137, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=28.8065843621399, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=55.7840616966581, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=55.7840616966581, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=51.2886597938144, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=28.3516483516484, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=28.3516483516484, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=28.3516483516484, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=28.3516483516484, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24652838, Length=454, Percent_Identity=28.1938325991189, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI17137572, Length=454, Percent_Identity=28.1938325991189, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=29.8013245033113, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137380, Length=347, Percent_Identity=29.3948126801153, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45550900, Length=441, Percent_Identity=26.530612244898, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647046, Length=378, Percent_Identity=26.984126984127, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647048, Length=378, Percent_Identity=26.984126984127, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28572034, Length=170, Percent_Identity=34.1176470588235, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43209; Mature: 43078

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKIYGGVAKDFASIDNAPEERERG
CCHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
ITISTSHVEYDTPARHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEEECCEECCCCHHHEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHH
LLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALGALN
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GEKEWEDKIVELAEALDSYIPEPERAVDQPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEEEHHHCCCEECC
DEVEIVGIKETTMTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLAAKGSIN
CEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCCC
PHTKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMT
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCEEEECCCCCEEE
VELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
EEEHHHHHHCCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
SKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKIYGGVAKDFASIDNAPEERERG
CHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
ITISTSHVEYDTPARHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEEECCEECCCCHHHEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHH
LLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALGALN
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GEKEWEDKIVELAEALDSYIPEPERAVDQPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEEEHHHCCCEECC
DEVEIVGIKETTMTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLAAKGSIN
CEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCCC
PHTKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMT
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCEEEECCCCCEEE
VELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
EEEHHHHHHCCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA