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The map label for this gene is poxA [H]

Identifier: 59712940

GI number: 59712940

Start: 2622401

End: 2623387

Strand: Reverse

Name: poxA [H]

Synonym: VF_2333

Alternate gene names: 59712940

Gene position: 2623387-2622401 (Counterclockwise)

Preceding gene: 59712945

Following gene: 59712936

Centisome position: 90.54

GC content: 39.51

Gene sequence:

>987_bases
ATGTGTAAGATTATGACTAGTCAAAACTGGATGCCTAGCGCATCAATTGAGCAATTACAGCAGCGTGCTGATATTTTAGC
GGCGATTCGTTCATTCTTTTTTAAGCGAAAAGTAATGGAAGTGGATACGCCGGCAATGAGTCATGCGACAGTAACGGATG
TGCATTTACATACTTTCCAAACAGACTTTATTGGACCGGGATATGCAGATGGCACTCATCTTTATTTCATGACTAGTCCA
GAGTTTCATATGAAGCGCTTATTAGCCGCTGGAAGCGGGTGTATTTACCAAATAAATAAAGCGTTTCGAAATGAAGAAGC
TGGCCGTTACCATAACCCTGAATTCACTATGTTAGAGTGGTATCGTTTAGGTTTTGATCATCATGACCTAATGGATGAAA
TGAATGAGTTATTAACGTTAGTGTTGAAATGTGACAATGCTGAACGTATGACTTATCAAGAAGCTTTTATTCAAGTACTT
GGTGTTTGCCCTTTAGAAGCGAGTATGGATGAATTAAGAGCGGTTGCTGCGCCATTAGGTTTATCTGATATTGCAGATAT
TGAAGAAGATCGTGACACTTTATTGCAGCTTTTATTTAGTATGGGAGTTGAAGTAAAAATTGGGCAAGATGTGCCTGCTT
TTGTTTATGATTTTCCTGCTTCTCAAGCGGCGTTAGCTAAAATTAATCCAAGCGATCCACGAGTAGCTGAACGATTTGAG
GTTTACTTTAAGGGAATTGAGTTAGCGAATGGCTTTCATGAGTTAGATAATGCAAAAGAGCAGCTCATGCGTTTTGAAGA
AGATAATGCAAAACGTAAATCAATGGGGCTAACTGAACAACCCATTGATTATCATTTGATCGCTGCGTTAGAAGCGGGTT
TGCCTAATTGTTCTGGCGTTGCTTTAGGTATTGATCGTTTAATCATGCTTGCGATGGGTGAGAAGCACATCGATCAAGTT
ACAGCATTTCCTTTTGATAGGGCATAA

Upstream 100 bases:

>100_bases
TTGGTTCGGTATCCCTTTGATGCTTAAGGGTATTTGTAAAGTTAAAAAATAGGTAACGTAAAATTGTGAGATAAGGTATT
ATTTCGCATAAGTTTTTCTA

Downstream 100 bases:

>100_bases
CTTCCCAGATTAAAGGTTATGAATCTAATGGTAAGATTAACTGACGATTAATCCCGTACCCATTACAGCTAAAAAAGCAC
CAAGCCATGCAAAGCGGTTC

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MCKIMTSQNWMPSASIEQLQQRADILAAIRSFFFKRKVMEVDTPAMSHATVTDVHLHTFQTDFIGPGYADGTHLYFMTSP
EFHMKRLLAAGSGCIYQINKAFRNEEAGRYHNPEFTMLEWYRLGFDHHDLMDEMNELLTLVLKCDNAERMTYQEAFIQVL
GVCPLEASMDELRAVAAPLGLSDIADIEEDRDTLLQLLFSMGVEVKIGQDVPAFVYDFPASQAALAKINPSDPRVAERFE
VYFKGIELANGFHELDNAKEQLMRFEEDNAKRKSMGLTEQPIDYHLIAALEAGLPNCSGVALGIDRLIMLAMGEKHIDQV
TAFPFDRA

Sequences:

>Translated_328_residues
MCKIMTSQNWMPSASIEQLQQRADILAAIRSFFFKRKVMEVDTPAMSHATVTDVHLHTFQTDFIGPGYADGTHLYFMTSP
EFHMKRLLAAGSGCIYQINKAFRNEEAGRYHNPEFTMLEWYRLGFDHHDLMDEMNELLTLVLKCDNAERMTYQEAFIQVL
GVCPLEASMDELRAVAAPLGLSDIADIEEDRDTLLQLLFSMGVEVKIGQDVPAFVYDFPASQAALAKINPSDPRVAERFE
VYFKGIELANGFHELDNAKEQLMRFEEDNAKRKSMGLTEQPIDYHLIAALEAGLPNCSGVALGIDRLIMLAMGEKHIDQV
TAFPFDRA
>Mature_328_residues
MCKIMTSQNWMPSASIEQLQQRADILAAIRSFFFKRKVMEVDTPAMSHATVTDVHLHTFQTDFIGPGYADGTHLYFMTSP
EFHMKRLLAAGSGCIYQINKAFRNEEAGRYHNPEFTMLEWYRLGFDHHDLMDEMNELLTLVLKCDNAERMTYQEAFIQVL
GVCPLEASMDELRAVAAPLGLSDIADIEEDRDTLLQLLFSMGVEVKIGQDVPAFVYDFPASQAALAKINPSDPRVAERFE
VYFKGIELANGFHELDNAKEQLMRFEEDNAKRKSMGLTEQPIDYHLIAALEAGLPNCSGVALGIDRLIMLAMGEKHIDQV
TAFPFDRA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=331, Percent_Identity=29.0030211480363, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI5031815, Length=331, Percent_Identity=29.0030211480363, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=60.5590062111801, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI1790571, Length=323, Percent_Identity=33.7461300309598, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1789256, Length=323, Percent_Identity=31.5789473684211, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17535927, Length=336, Percent_Identity=28.2738095238095, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994340, Length=336, Percent_Identity=28.2738095238095, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17535925, Length=336, Percent_Identity=28.2738095238095, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320242, Length=340, Percent_Identity=30, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324256, Length=349, Percent_Identity=27.2206303724928, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24640851, Length=340, Percent_Identity=27.6470588235294, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24640849, Length=340, Percent_Identity=27.6470588235294, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 37071; Mature: 37071

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKIMTSQNWMPSASIEQLQQRADILAAIRSFFFKRKVMEVDTPAMSHATVTDVHLHTFQ
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEE
TDFIGPGYADGTHLYFMTSPEFHMKRLLAAGSGCIYQINKAFRNEEAGRYHNPEFTMLEW
CCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCCCCHHHHH
YRLGFDHHDLMDEMNELLTLVLKCDNAERMTYQEAFIQVLGVCPLEASMDELRAVAAPLG
HHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
LSDIADIEEDRDTLLQLLFSMGVEVKIGQDVPAFVYDFPASQAALAKINPSDPRVAERFE
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHEEECCCCCCEEEECCCCCCHHHHHHH
VYFKGIELANGFHELDNAKEQLMRFEEDNAKRKSMGLTEQPIDYHLIAALEAGLPNCSGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCH
ALGIDRLIMLAMGEKHIDQVTAFPFDRA
HHHHHHHHHHHHCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCKIMTSQNWMPSASIEQLQQRADILAAIRSFFFKRKVMEVDTPAMSHATVTDVHLHTFQ
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEE
TDFIGPGYADGTHLYFMTSPEFHMKRLLAAGSGCIYQINKAFRNEEAGRYHNPEFTMLEW
CCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCCCCHHHHH
YRLGFDHHDLMDEMNELLTLVLKCDNAERMTYQEAFIQVLGVCPLEASMDELRAVAAPLG
HHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
LSDIADIEEDRDTLLQLLFSMGVEVKIGQDVPAFVYDFPASQAALAKINPSDPRVAERFE
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHEEECCCCCCEEEECCCCCCHHHHHHH
VYFKGIELANGFHELDNAKEQLMRFEEDNAKRKSMGLTEQPIDYHLIAALEAGLPNCSGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCH
ALGIDRLIMLAMGEKHIDQVTAFPFDRA
HHHHHHHHHHHHCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA