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The map label for this gene is miaA [H]

Identifier: 59712931

GI number: 59712931

Start: 2610777

End: 2611709

Strand: Reverse

Name: miaA [H]

Synonym: VF_2324

Alternate gene names: 59712931

Gene position: 2611709-2610777 (Counterclockwise)

Preceding gene: 59712932

Following gene: 59712930

Centisome position: 90.14

GC content: 41.91

Gene sequence:

>933_bases
ATGAATAAACCATTACCAAAAGCCATCTTTTTGATGGGACCAACAGCGTCAGGGAAAACAAACTTAGCAATTGAATTGCG
AAAACGTTTTCCTGTGGAGTTGATTAGCGTAGACTCCGCCCTTATCTATAAGGGTATGGATATTGGTACGGCAAAACCAA
ATGCAGAGGAACTTTTACAGGCTCCTCATCGTCTTATTGATATTCTTGATCCAACAGAGAGTTATTCGGCAGCGGATTTT
CGTCGTGATGCTCTTAAAGAGATGGATGATATCGTTGCTCAAGGAAAGATCCCATTGTTAGTGGGTGGTACGATGTTGTA
CTACAAAGCATTACTTGAAGGTCTTTCGCCATTACCAGCAGCGGATGCTGACATTAGAGCTCAAATTGAGCAAGAAGCGG
AACAATTTGGATGGGAAGCGATGCATGATCAATTAAAAACGATTGATCCTGTCTCAGCTGAAAGAATTCATCCAAATGAT
CCACAACGACTTTCCCGAGCGTTGGAAGTATTTCGAATTTCGGGTAAAACATTAACAGAATTAACCCAGATTAAAGGTGA
TGCTTTGCCTTATGAGGTGCATCAATTTGCGATTGCACCGAAAGAGAGAGCAGAAATTCATCGTCGTATTGAACTTCGCT
TTGCGAATATGATGGAAGAAGGGTTTGAAGCAGAAGCACGAGCATTGTATGAACGTGATGATCTTCATGCTGATTTACCA
TCAATTCGATGTGTTGGTTATCGACAAATGTGGGACTATTTTGACGGGGAGGGGACATTGGATGAGGCGGTTTTCCGTGG
TATCTGTGCTACTCGTCAATTAGCCAAGCGACAAATTACCTGGCTACGTAGCTGGAAAGAGTTAACTTGGCTTGATAGTG
ATGACATTGACGGCGCTTTAGAGTTGATTTCGACTCAATTAGAGAAAAAATAA

Upstream 100 bases:

>100_bases
GTGATGGAATTAGAGCAATTATGGGGAGATGAATTGAATAATTTCTACCCTAAATTATTAAAAGACATCGATATTACACA
AGTGATACAAGCATTTAGTC

Downstream 100 bases:

>100_bases
GCACAAAAATGCTTCAATGTTGTTTAACTTAAGTGCGTTTAGTCATAAGCTAAAGGCTCATAGCTAAATAAAATTACAAT
TACAAACCAAAAAGGAAAAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNKPLPKAIFLMGPTASGKTNLAIELRKRFPVELISVDSALIYKGMDIGTAKPNAEELLQAPHRLIDILDPTESYSAADF
RRDALKEMDDIVAQGKIPLLVGGTMLYYKALLEGLSPLPAADADIRAQIEQEAEQFGWEAMHDQLKTIDPVSAERIHPND
PQRLSRALEVFRISGKTLTELTQIKGDALPYEVHQFAIAPKERAEIHRRIELRFANMMEEGFEAEARALYERDDLHADLP
SIRCVGYRQMWDYFDGEGTLDEAVFRGICATRQLAKRQITWLRSWKELTWLDSDDIDGALELISTQLEKK

Sequences:

>Translated_310_residues
MNKPLPKAIFLMGPTASGKTNLAIELRKRFPVELISVDSALIYKGMDIGTAKPNAEELLQAPHRLIDILDPTESYSAADF
RRDALKEMDDIVAQGKIPLLVGGTMLYYKALLEGLSPLPAADADIRAQIEQEAEQFGWEAMHDQLKTIDPVSAERIHPND
PQRLSRALEVFRISGKTLTELTQIKGDALPYEVHQFAIAPKERAEIHRRIELRFANMMEEGFEAEARALYERDDLHADLP
SIRCVGYRQMWDYFDGEGTLDEAVFRGICATRQLAKRQITWLRSWKELTWLDSDDIDGALELISTQLEKK
>Mature_310_residues
MNKPLPKAIFLMGPTASGKTNLAIELRKRFPVELISVDSALIYKGMDIGTAKPNAEELLQAPHRLIDILDPTESYSAADF
RRDALKEMDDIVAQGKIPLLVGGTMLYYKALLEGLSPLPAADADIRAQIEQEAEQFGWEAMHDQLKTIDPVSAERIHPND
PQRLSRALEVFRISGKTLTELTQIKGDALPYEVHQFAIAPKERAEIHRRIELRFANMMEEGFEAEARALYERDDLHADLP
SIRCVGYRQMWDYFDGEGTLDEAVFRGICATRQLAKRQITWLRSWKELTWLDSDDIDGALELISTQLEKK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=317, Percent_Identity=27.4447949526814, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1790613, Length=290, Percent_Identity=76.551724137931, Blast_Score=469, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25144712, Length=297, Percent_Identity=28.6195286195286, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=27.3356401384083, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24649830, Length=182, Percent_Identity=34.6153846153846, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35151; Mature: 35151

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPLPKAIFLMGPTASGKTNLAIELRKRFPVELISVDSALIYKGMDIGTAKPNAEELLQ
CCCCCCCEEEEECCCCCCCCCEEEEEHHHCCEEEEECCHHHHHCCCCCCCCCCCHHHHHH
APHRLIDILDPTESYSAADFRRDALKEMDDIVAQGKIPLLVGGTMLYYKALLEGLSPLPA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCC
ADADIRAQIEQEAEQFGWEAMHDQLKTIDPVSAERIHPNDPQRLSRALEVFRISGKTLTE
CCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
LTQIKGDALPYEVHQFAIAPKERAEIHRRIELRFANMMEEGFEAEARALYERDDLHADLP
HHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
SIRCVGYRQMWDYFDGEGTLDEAVFRGICATRQLAKRQITWLRSWKELTWLDSDDIDGAL
CHHEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ELISTQLEKK
HHHHHHHCCC
>Mature Secondary Structure
MNKPLPKAIFLMGPTASGKTNLAIELRKRFPVELISVDSALIYKGMDIGTAKPNAEELLQ
CCCCCCCEEEEECCCCCCCCCEEEEEHHHCCEEEEECCHHHHHCCCCCCCCCCCHHHHHH
APHRLIDILDPTESYSAADFRRDALKEMDDIVAQGKIPLLVGGTMLYYKALLEGLSPLPA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCC
ADADIRAQIEQEAEQFGWEAMHDQLKTIDPVSAERIHPNDPQRLSRALEVFRISGKTLTE
CCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
LTQIKGDALPYEVHQFAIAPKERAEIHRRIELRFANMMEEGFEAEARALYERDDLHADLP
HHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
SIRCVGYRQMWDYFDGEGTLDEAVFRGICATRQLAKRQITWLRSWKELTWLDSDDIDGAL
CHHEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ELISTQLEKK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA