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The map label for this gene is rlmB [H]

Identifier: 59712920

GI number: 59712920

Start: 2599037

End: 2599783

Strand: Reverse

Name: rlmB [H]

Synonym: VF_2313

Alternate gene names: 59712920

Gene position: 2599783-2599037 (Counterclockwise)

Preceding gene: 59712921

Following gene: 59712919

Centisome position: 89.72

GC content: 42.17

Gene sequence:

>747_bases
ATGAGTAATGATTATATTTTTGGTATCCACGCAGCCAAAGCAGTACTAGAGCGTGATCCTGCCCGTTTTATTGAAGTATT
TGTATTAAAAGGTCGTCAAGATGATCGTTTATTACCAATCTTGAATGAATTAACGCAGTTGGGTTTTAAGATTCAAGAAC
TGCCACGTAAAACGTTGGATGACAAAGCAAATGGTGCCAATCACCAAGGAATTATTGCTCGTGTAACGCCAGCTAAGCAA
CTGAATGAACATGATTTAGACGATATCGTGTCTCAAACAGAGAATCCATTATTGCTTGTTCTTGATGGTGTGACTGATCC
TCACAACCTAGGTGCATGTTTACGTAATGCCGATGCGGCAGGTGTAGCTGCGGTTATCGTACCAAAAGACAAATCTGCAT
CAATGACAGCAACCGTAAGTAAAGTTGCATGTGGTGCGGCTGAAACGGTTCCTTTAGTACGTGTAACTAACCTAGCTCGT
ACAATGCGTGCATTACAAGAAAAGGGCGTATGGTTTGTTGGGACTGCTGGTGAGGCAACTCATGATGTATTCCAGTCTAA
ATTAACTGGTCCTTTAGCAATAGTGATGGGTGCAGAACGGAGATGGCATGCGTCGCTTAAACACGTGAAAACGTGTGATG
ACCTAATTAAAATCCCAATGGCTGGCTCTGTATCAAGTTTAAAAGTTTCTGTAGCGTCTGGTATCTGTTTATTTGAAGCA
GTACGCCAACGTGCGTTACAAGGCTAA

Upstream 100 bases:

>100_bases
AGTTAGAGCAAGAAAGCCAAAGGCAAATAAGTAATTTCGATAATGGGGCGGTTATAGCCCCATTTAGATAAACAAATAAG
TAAAGAAAAGAAGCGACAGC

Downstream 100 bases:

>100_bases
ATAAGTAGGCATTTAGGTGCTAATTGTCTGATTAGCATTATGAAATAAGCTCAAAAAAGAGGATGTTTTTTCATTCTCTT
TTTTTCTTGGTAAAGACGAA

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSNDYIFGIHAAKAVLERDPARFIEVFVLKGRQDDRLLPILNELTQLGFKIQELPRKTLDDKANGANHQGIIARVTPAKQ
LNEHDLDDIVSQTENPLLLVLDGVTDPHNLGACLRNADAAGVAAVIVPKDKSASMTATVSKVACGAAETVPLVRVTNLAR
TMRALQEKGVWFVGTAGEATHDVFQSKLTGPLAIVMGAERRWHASLKHVKTCDDLIKIPMAGSVSSLKVSVASGICLFEA
VRQRALQG

Sequences:

>Translated_248_residues
MSNDYIFGIHAAKAVLERDPARFIEVFVLKGRQDDRLLPILNELTQLGFKIQELPRKTLDDKANGANHQGIIARVTPAKQ
LNEHDLDDIVSQTENPLLLVLDGVTDPHNLGACLRNADAAGVAAVIVPKDKSASMTATVSKVACGAAETVPLVRVTNLAR
TMRALQEKGVWFVGTAGEATHDVFQSKLTGPLAIVMGAERRWHASLKHVKTCDDLIKIPMAGSVSSLKVSVASGICLFEA
VRQRALQG
>Mature_247_residues
SNDYIFGIHAAKAVLERDPARFIEVFVLKGRQDDRLLPILNELTQLGFKIQELPRKTLDDKANGANHQGIIARVTPAKQL
NEHDLDDIVSQTENPLLLVLDGVTDPHNLGACLRNADAAGVAAVIVPKDKSASMTATVSKVACGAAETVPLVRVTNLART
MRALQEKGVWFVGTAGEATHDVFQSKLTGPLAIVMGAERRWHASLKHVKTCDDLIKIPMAGSVSSLKVSVASGICLFEAV
RQRALQG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=206, Percent_Identity=30.5825242718447, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=65.0205761316872, Blast_Score=327, Evalue=5e-91,
Organism=Escherichia coli, GI1788935, Length=184, Percent_Identity=28.804347826087, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790083, Length=160, Percent_Identity=30.625, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26731; Mature: 26600

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDYIFGIHAAKAVLERDPARFIEVFVLKGRQDDRLLPILNELTQLGFKIQELPRKTLD
CCCCEEEEHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHC
DKANGANHQGIIARVTPAKQLNEHDLDDIVSQTENPLLLVLDGVTDPHNLGACLRNADAA
CCCCCCCCCCEEEEECCHHHCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCC
GVAAVIVPKDKSASMTATVSKVACGAAETVPLVRVTNLARTMRALQEKGVWFVGTAGEAT
CEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHH
HDVFQSKLTGPLAIVMGAERRWHASLKHVKTCDDLIKIPMAGSVSSLKVSVASGICLFEA
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEHHHHHHHHHHH
VRQRALQG
HHHHHCCC
>Mature Secondary Structure 
SNDYIFGIHAAKAVLERDPARFIEVFVLKGRQDDRLLPILNELTQLGFKIQELPRKTLD
CCCEEEEHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHC
DKANGANHQGIIARVTPAKQLNEHDLDDIVSQTENPLLLVLDGVTDPHNLGACLRNADAA
CCCCCCCCCCEEEEECCHHHCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCC
GVAAVIVPKDKSASMTATVSKVACGAAETVPLVRVTNLARTMRALQEKGVWFVGTAGEAT
CEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHH
HDVFQSKLTGPLAIVMGAERRWHASLKHVKTCDDLIKIPMAGSVSSLKVSVASGICLFEA
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEHHHHHHHHHHH
VRQRALQG
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]