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The map label for this gene is argH

Identifier: 59712910

GI number: 59712910

Start: 2586654

End: 2588528

Strand: Reverse

Name: argH

Synonym: VF_2303

Alternate gene names: 59712910

Gene position: 2588528-2586654 (Counterclockwise)

Preceding gene: 59712911

Following gene: 59712906

Centisome position: 89.34

GC content: 42.51

Gene sequence:

>1875_bases
ATGGCATTATGGGGCGGACGTTTTAGTCAAGCAGCAGACACCAGATTCAAACAGTTTAACGATTCACTTCGTATTGATTA
CCGCCTTGCAGAACAAGATATTGTTGGTTCGATTGCATGGTCTAAAGCATTGCTTTCAGTAGGTGTAATTACCGAACTTG
AGCAGCAAAAGCTTGAATTAGCACTGAATGAATTAAAATTAGAAGTAATGGAAGATCCTGAACAGATCTTATTGTCTGAT
GCTGAAGATATTCACAGTTGGGTAGAAACTCAATTGATTGGTAAAGTCGGTGACTTAGGTAAAAAACTCCATACTGGCCG
TTCTCGTAATGATCAAGTAGCAACAGATTTAAAGCTTTGGTGTCGTCAACAAGGACATCAACTACTGCGTACACTTGATC
AACTTCTTAATCAATTAGTAAACGTAGCAAGTGAGCATCAAGCGACAGTACTTCCGGGTTATACGCACTTACAACGTGCA
CAGCCTGTGACTTTTGCTCATTGGTGTTTAGCTTATGTTGAGATGATTGAGCGTGATTATTCTCGTCTTGAAGATGCAAT
TAATCGTTTAGATACTTGTCCGTTAGGCTCTGGAGCTCTTGCTGGCACTGCTTATCCTATGGATCGTGAAAAGCTGGCTC
GTAACCTTGGATTCCAACGTGCAACACGTAATAGTTTAGATTCGGTATCTGATCGTGATCATGTCATGGAACTCATGTCG
ATCGCTTCCATTTCAATGTTGCACCTTTCTCGTCTTGCTGAAGACATGATTTTTTACAATTCAGGTGAATCGAATTTTAT
CGAATTGGCTGATACCGTGACTTCAGGCTCATCACTTATGCCGCAGAAGAAAAACCCAGATGCATTGGAGTTGATCCGTG
GTAAGTGTGGACGTGTATATGGTGCAATGGCTGGCATGATGATGACAGTAAAAGCATTGCCACTGGCTTACAACAAAGAC
ATGCAAGAAGACAAAGAAGGCTTATTTGATGCCTTGGATAGCTGGTCTGATTGTATTGAAATGGCTGCGTTGTGCTTTGA
AGGTATTAAGATTAATGGGGAACGTACGCTTGAAGCAGCAAAACAAGGGTATGCAAACTCAACAGAACTGGCTGATTACT
TGGTAGCGAAAGGCATTCCGTTCCGTGAAGCTCACCATATTGTTGGTGTTGCCGTTGTCGGTGCGATTGAAAAAGGCTGT
GCATTAGAAGAACTTTCTCTTGCTGAACTGAAAGCGTTCTCTGAAGTGATTGAAGACGATGTGTATGAAATCCTCACCAT
TGAATCTTGCCTTGCAAAACGTTGTGCATTAGGTGGCGTTGCTCCTGAGCAAGTTGCGTATGCCGTTGAGCAAGCAGGTA
AACGTCTACAAGAGCGTGATAATGCTGGTGTGAAGGTTCGTCCAGCTCGTTTAACTGATTTAGATGCACTGGAAGGTATG
GTTGCATACTGGGCTGGGCTTGGTGAAAACTTACCGCGTAATCGTAATGAATTAGTACGTGATATTGGCTCATTTGCTGT
GGCAGAGCATCATGGAGAAGTGACAGGCTGTGCCTCTTTGTATGTGTATGACTCAGGACTTGCAGAAATTCGTTCATTAG
GTGTTGAAGCTGGGTGGCAGAGTCAAGGTCAAGGTAAAGCCATTGTTCAGCATTTAGTTGAAAAAGCACGTGAGATGGCG
ATTAAGAAAGTTTTTGTATTAACTCGTGTTCCAGAGTTCTTTATGAAACAAGACTTTATTCCTACCTCTCGTTCATTGTT
GCCAGAAAAGGTGCTGAAAGACTGTGATCAATGTCCACGTCAACATGCGTGTGATGAGGTTGCACTAGAAGTGAATCTAC
AAGAGCAAGTGATCATTAAAAGCAGTGTAGCTTAG

Upstream 100 bases:

>100_bases
TTATTTTTATCTGTAATTACCGTAAGTTAAAACCATCAAGAAAAATACTGAGTTTAATCAGAACAAAAGCAGTGAGCATT
GTGCATTTAGGAGAGACAGC

Downstream 100 bases:

>100_bases
TTTATATTGCAAATATTTATGCGATTGAGTTAACTAAATAAGCGTAATAAAAAAGAAAAAGTGGTGAACTTTTTAATTAA
GTATTAGTCTTATTAATACT

Product: bifunctional argininosuccinate lyase/N-acetylglutamate synthase

Products: NA

Alternate protein names: Argininosuccinate lyase; ASAL; Arginosuccinase; Probable acetyltransferase

Number of amino acids: Translated: 624; Mature: 623

Protein sequence:

>624_residues
MALWGGRFSQAADTRFKQFNDSLRIDYRLAEQDIVGSIAWSKALLSVGVITELEQQKLELALNELKLEVMEDPEQILLSD
AEDIHSWVETQLIGKVGDLGKKLHTGRSRNDQVATDLKLWCRQQGHQLLRTLDQLLNQLVNVASEHQATVLPGYTHLQRA
QPVTFAHWCLAYVEMIERDYSRLEDAINRLDTCPLGSGALAGTAYPMDREKLARNLGFQRATRNSLDSVSDRDHVMELMS
IASISMLHLSRLAEDMIFYNSGESNFIELADTVTSGSSLMPQKKNPDALELIRGKCGRVYGAMAGMMMTVKALPLAYNKD
MQEDKEGLFDALDSWSDCIEMAALCFEGIKINGERTLEAAKQGYANSTELADYLVAKGIPFREAHHIVGVAVVGAIEKGC
ALEELSLAELKAFSEVIEDDVYEILTIESCLAKRCALGGVAPEQVAYAVEQAGKRLQERDNAGVKVRPARLTDLDALEGM
VAYWAGLGENLPRNRNELVRDIGSFAVAEHHGEVTGCASLYVYDSGLAEIRSLGVEAGWQSQGQGKAIVQHLVEKAREMA
IKKVFVLTRVPEFFMKQDFIPTSRSLLPEKVLKDCDQCPRQHACDEVALEVNLQEQVIIKSSVA

Sequences:

>Translated_624_residues
MALWGGRFSQAADTRFKQFNDSLRIDYRLAEQDIVGSIAWSKALLSVGVITELEQQKLELALNELKLEVMEDPEQILLSD
AEDIHSWVETQLIGKVGDLGKKLHTGRSRNDQVATDLKLWCRQQGHQLLRTLDQLLNQLVNVASEHQATVLPGYTHLQRA
QPVTFAHWCLAYVEMIERDYSRLEDAINRLDTCPLGSGALAGTAYPMDREKLARNLGFQRATRNSLDSVSDRDHVMELMS
IASISMLHLSRLAEDMIFYNSGESNFIELADTVTSGSSLMPQKKNPDALELIRGKCGRVYGAMAGMMMTVKALPLAYNKD
MQEDKEGLFDALDSWSDCIEMAALCFEGIKINGERTLEAAKQGYANSTELADYLVAKGIPFREAHHIVGVAVVGAIEKGC
ALEELSLAELKAFSEVIEDDVYEILTIESCLAKRCALGGVAPEQVAYAVEQAGKRLQERDNAGVKVRPARLTDLDALEGM
VAYWAGLGENLPRNRNELVRDIGSFAVAEHHGEVTGCASLYVYDSGLAEIRSLGVEAGWQSQGQGKAIVQHLVEKAREMA
IKKVFVLTRVPEFFMKQDFIPTSRSLLPEKVLKDCDQCPRQHACDEVALEVNLQEQVIIKSSVA
>Mature_623_residues
ALWGGRFSQAADTRFKQFNDSLRIDYRLAEQDIVGSIAWSKALLSVGVITELEQQKLELALNELKLEVMEDPEQILLSDA
EDIHSWVETQLIGKVGDLGKKLHTGRSRNDQVATDLKLWCRQQGHQLLRTLDQLLNQLVNVASEHQATVLPGYTHLQRAQ
PVTFAHWCLAYVEMIERDYSRLEDAINRLDTCPLGSGALAGTAYPMDREKLARNLGFQRATRNSLDSVSDRDHVMELMSI
ASISMLHLSRLAEDMIFYNSGESNFIELADTVTSGSSLMPQKKNPDALELIRGKCGRVYGAMAGMMMTVKALPLAYNKDM
QEDKEGLFDALDSWSDCIEMAALCFEGIKINGERTLEAAKQGYANSTELADYLVAKGIPFREAHHIVGVAVVGAIEKGCA
LEELSLAELKAFSEVIEDDVYEILTIESCLAKRCALGGVAPEQVAYAVEQAGKRLQERDNAGVKVRPARLTDLDALEGMV
AYWAGLGENLPRNRNELVRDIGSFAVAEHHGEVTGCASLYVYDSGLAEIRSLGVEAGWQSQGQGKAIVQHLVEKAREMAI
KKVFVLTRVPEFFMKQDFIPTSRSLLPEKVLKDCDQCPRQHACDEVALEVNLQEQVIIKSSVA

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain

Homologues:

Organism=Homo sapiens, GI31541964, Length=455, Percent_Identity=43.7362637362637, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI68303542, Length=455, Percent_Identity=43.7362637362637, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI68303549, Length=455, Percent_Identity=41.978021978022, Blast_Score=332, Evalue=9e-91,
Organism=Homo sapiens, GI68303547, Length=455, Percent_Identity=41.0989010989011, Blast_Score=324, Evalue=2e-88,
Organism=Escherichia coli, GI1790398, Length=456, Percent_Identity=71.9298245614035, Blast_Score=675, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321806, Length=446, Percent_Identity=42.8251121076233, Blast_Score=355, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6324993, Length=330, Percent_Identity=25.4545454545455, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI221473854, Length=449, Percent_Identity=42.0935412026726, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI78706858, Length=449, Percent_Identity=42.0935412026726, Blast_Score=350, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_VIBF1 (Q5E2E8)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205686.1
- ProteinModelPortal:   Q5E2E8
- STRING:   Q5E2E8
- GeneID:   3279570
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2303
- NMPDR:   fig|312309.3.peg.2303
- eggNOG:   COG0165
- HOGENOM:   HBG539632
- OMA:   MAEDLIF
- PhylomeDB:   Q5E2E8
- ProtClustDB:   PRK12308
- BioCyc:   VFIS312309:VF2303-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR009049
- InterPro:   IPR011244
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- Gene3D:   G3DSA:3.40.630.30
- PANTHER:   PTHR11444:SF3
- PIRSF:   PIRSF036456
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00583 Acetyltransf_1; PF00206 Lyase_1; SSF55729 Acyl_CoA_acyltransferase; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 69177; Mature: 69046

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00163 FUMARATE_LYASES; PS51186 GNAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALWGGRFSQAADTRFKQFNDSLRIDYRLAEQDIVGSIAWSKALLSVGVITELEQQKLEL
CCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALNELKLEVMEDPEQILLSDAEDIHSWVETQLIGKVGDLGKKLHTGRSRNDQVATDLKLW
HHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
CRQQGHQLLRTLDQLLNQLVNVASEHQATVLPGYTHLQRAQPVTFAHWCLAYVEMIERDY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
SRLEDAINRLDTCPLGSGALAGTAYPMDREKLARNLGFQRATRNSLDSVSDRDHVMELMS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHH
IASISMLHLSRLAEDMIFYNSGESNFIELADTVTSGSSLMPQKKNPDALELIRGKCGRVY
HHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
GAMAGMMMTVKALPLAYNKDMQEDKEGLFDALDSWSDCIEMAALCFEGIKINGERTLEAA
HHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHHHHH
KQGYANSTELADYLVAKGIPFREAHHIVGVAVVGAIEKGCALEELSLAELKAFSEVIEDD
HCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VYEILTIESCLAKRCALGGVAPEQVAYAVEQAGKRLQERDNAGVKVRPARLTDLDALEGM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHH
VAYWAGLGENLPRNRNELVRDIGSFAVAEHHGEVTGCASLYVYDSGLAEIRSLGVEAGWQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCHHHHHHHCCCCCCC
SQGQGKAIVQHLVEKAREMAIKKVFVLTRVPEFFMKQDFIPTSRSLLPEKVLKDCDQCPR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHCHHHHHHHHHHCHH
QHACDEVALEVNLQEQVIIKSSVA
HHCCCHHEEEECCHHHHHHHHCCC
>Mature Secondary Structure 
ALWGGRFSQAADTRFKQFNDSLRIDYRLAEQDIVGSIAWSKALLSVGVITELEQQKLEL
CCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALNELKLEVMEDPEQILLSDAEDIHSWVETQLIGKVGDLGKKLHTGRSRNDQVATDLKLW
HHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
CRQQGHQLLRTLDQLLNQLVNVASEHQATVLPGYTHLQRAQPVTFAHWCLAYVEMIERDY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
SRLEDAINRLDTCPLGSGALAGTAYPMDREKLARNLGFQRATRNSLDSVSDRDHVMELMS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHH
IASISMLHLSRLAEDMIFYNSGESNFIELADTVTSGSSLMPQKKNPDALELIRGKCGRVY
HHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
GAMAGMMMTVKALPLAYNKDMQEDKEGLFDALDSWSDCIEMAALCFEGIKINGERTLEAA
HHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHHHHH
KQGYANSTELADYLVAKGIPFREAHHIVGVAVVGAIEKGCALEELSLAELKAFSEVIEDD
HCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VYEILTIESCLAKRCALGGVAPEQVAYAVEQAGKRLQERDNAGVKVRPARLTDLDALEGM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHH
VAYWAGLGENLPRNRNELVRDIGSFAVAEHHGEVTGCASLYVYDSGLAEIRSLGVEAGWQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCHHHHHHHCCCCCCC
SQGQGKAIVQHLVEKAREMAIKKVFVLTRVPEFFMKQDFIPTSRSLLPEKVLKDCDQCPR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHCHHHHHHHHHHCHH
QHACDEVALEVNLQEQVIIKSSVA
HHCCCHHEEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA