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The map label for this gene is argG [H]

Identifier: 59712911

GI number: 59712911

Start: 2588826

End: 2590037

Strand: Reverse

Name: argG [H]

Synonym: VF_2304

Alternate gene names: 59712911

Gene position: 2590037-2588826 (Counterclockwise)

Preceding gene: 59712912

Following gene: 59712910

Centisome position: 89.39

GC content: 42.0

Gene sequence:

>1212_bases
ATGAGTAAGAAAGTAGAAGTAAACAAGGTTGTTGTCGCATACTCTGGTGGCTTAGATACCTCTGTGATCATCCCATGGTT
AAAAGAGAATTATAACTGTGAAGTGATTGCTTTTGTTGCGGATGTTGGTCAAGGCGAAGAAGAACTTGAAGGTATTGAAG
CGAAGGCAATTGCATCGGGGGCGACTGAGTGTTATGTCGTTGATTTAAAAGAAGAAATGGTTTCTGAGTATATTTTCCCA
ACGCTAAAAACAGGCGCGTTGTATGAAGGTAAATATTTACTAGGAACGTCAATGGCACGTCCTATCATTGCTAAAGCACA
AGTTGAAGTGGCTCGTAATGTGGGTGCAGATGCGCTTTGTCATGGTTGTACTGGTAAAGGTAATGACCAGATTCGTTTTG
AAGGTGCGTTTGCTGCGCTAGCTCCAGATCTTCATGTTATTGCTCCATGGCGTGAGTGGGATCTTGTGAGCCGTGAAGAG
TGTTTAGATTATTTGGCTGAGCGTAATATCCCTTGTACTGCTTCTCTTACTAAGATCTATTCTCGTGATGCTAATGCATG
GCATATCTCAACTGAAGGTGGTGTTTTAGAAGAAACATGGAATGCACCAAATGACGATTGTTGGGCATGGACTGTTGATC
CTGAGCAAGCACCAAATGAATCAGAAACGATCTCATTAAAAGTAGAAAAGGGTGCGGTTGTTGCGGTTGATGGTAAAGCG
ATGACACCTTATGAAGTGGTTGTATACCTAAATGAGAAAGGGGCAAAGCACGGTGTTGGTCGTATTGATATCGTTGAAAA
CCGTTTGGTAGGAATGAAGTCTCGTGGTTGTTATGAAACTCCAGGGGGCACGATCATTAACGAAGCATTGCGTGCAGTTG
AACAACTTGTTTTAGATAAAACGTCATTTGAATTCCGCGAAGAGCTAGGCATTAAAGCCTCTCACCTTGTTTATGATGGT
CGTTGGTTTACACCGTTATGTAAGTCTATCTTAGCGGCATCTGAAGAGCTGGCAAAAGACGTGAATGGCGAGGTCGTTAT
TAAGTTGTATAAAGGCCATGCGACGGTAATTCAAAAGCGTTCTGATAACAGTTTATATTCAGAAGAGTTTGCTACTTTTG
GTGCCGATGAAGTGTATGACCAAAGTCATGCAGAAGGCTTTATTCGTCTTTACTCGTTATCAAGTCGTATTCGTGCGCTA
AATAGCAAATAA

Upstream 100 bases:

>100_bases
TGTTTATTGGAAACAGCATTGGCACACAATTTCAGCCTCAATAGGCAATGTAATTTTAAGAATATAGTCGCCGCGAGTTG
TGCGGTTCAAGGAGAATAGA

Downstream 100 bases:

>100_bases
GTAATGCGTATTACTTATTAAGAATAAGATTTAAACAATTTGTTTTACACTATGTCGAAGCTCGTTTTATATAAACGGGC
TTCGATTTTTTGTATGTAAC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSKKVEVNKVVVAYSGGLDTSVIIPWLKENYNCEVIAFVADVGQGEEELEGIEAKAIASGATECYVVDLKEEMVSEYIFP
TLKTGALYEGKYLLGTSMARPIIAKAQVEVARNVGADALCHGCTGKGNDQIRFEGAFAALAPDLHVIAPWREWDLVSREE
CLDYLAERNIPCTASLTKIYSRDANAWHISTEGGVLEETWNAPNDDCWAWTVDPEQAPNESETISLKVEKGAVVAVDGKA
MTPYEVVVYLNEKGAKHGVGRIDIVENRLVGMKSRGCYETPGGTIINEALRAVEQLVLDKTSFEFREELGIKASHLVYDG
RWFTPLCKSILAASEELAKDVNGEVVIKLYKGHATVIQKRSDNSLYSEEFATFGADEVYDQSHAEGFIRLYSLSSRIRAL
NSK

Sequences:

>Translated_403_residues
MSKKVEVNKVVVAYSGGLDTSVIIPWLKENYNCEVIAFVADVGQGEEELEGIEAKAIASGATECYVVDLKEEMVSEYIFP
TLKTGALYEGKYLLGTSMARPIIAKAQVEVARNVGADALCHGCTGKGNDQIRFEGAFAALAPDLHVIAPWREWDLVSREE
CLDYLAERNIPCTASLTKIYSRDANAWHISTEGGVLEETWNAPNDDCWAWTVDPEQAPNESETISLKVEKGAVVAVDGKA
MTPYEVVVYLNEKGAKHGVGRIDIVENRLVGMKSRGCYETPGGTIINEALRAVEQLVLDKTSFEFREELGIKASHLVYDG
RWFTPLCKSILAASEELAKDVNGEVVIKLYKGHATVIQKRSDNSLYSEEFATFGADEVYDQSHAEGFIRLYSLSSRIRAL
NSK
>Mature_402_residues
SKKVEVNKVVVAYSGGLDTSVIIPWLKENYNCEVIAFVADVGQGEEELEGIEAKAIASGATECYVVDLKEEMVSEYIFPT
LKTGALYEGKYLLGTSMARPIIAKAQVEVARNVGADALCHGCTGKGNDQIRFEGAFAALAPDLHVIAPWREWDLVSREEC
LDYLAERNIPCTASLTKIYSRDANAWHISTEGGVLEETWNAPNDDCWAWTVDPEQAPNESETISLKVEKGAVVAVDGKAM
TPYEVVVYLNEKGAKHGVGRIDIVENRLVGMKSRGCYETPGGTIINEALRAVEQLVLDKTSFEFREELGIKASHLVYDGR
WFTPLCKSILAASEELAKDVNGEVVIKLYKGHATVIQKRSDNSLYSEEFATFGADEVYDQSHAEGFIRLYSLSSRIRALN
SK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=393, Percent_Identity=42.2391857506361, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI16950633, Length=393, Percent_Identity=42.2391857506361, Blast_Score=303, Evalue=3e-82,
Organism=Escherichia coli, GI1789563, Length=404, Percent_Identity=25.990099009901, Blast_Score=133, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=400, Percent_Identity=39.25, Blast_Score=282, Evalue=7e-77,
Organism=Drosophila melanogaster, GI21358151, Length=408, Percent_Identity=38.9705882352941, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44393; Mature: 44261

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKVEVNKVVVAYSGGLDTSVIIPWLKENYNCEVIAFVADVGQGEEELEGIEAKAIASG
CCCEEEEEEEEEEEECCCCCEEEEEHHHCCCCEEEEEEEECCCCCHHHHCCCCHHHHHCC
ATECYVVDLKEEMVSEYIFPTLKTGALYEGKYLLGTSMARPIIAKAQVEVARNVGADALC
CCEEEEEECHHHHHHHHHCCCCCCCCEECCCEEECCHHCCCHHHHHHHHHHHCCCHHHHE
HGCTGKGNDQIRFEGAFAALAPDLHVIAPWREWDLVSREECLDYLAERNIPCTASLTKIY
ECCCCCCCCEEEEECEEEECCCCCEEECCCHHCCCCCHHHHHHHHHHCCCCCCHHHHHHH
SRDANAWHISTEGGVLEETWNAPNDDCWAWTVDPEQAPNESETISLKVEKGAVVAVDGKA
CCCCCEEEEECCCCEEHHCCCCCCCCEEEEEECHHHCCCCCCEEEEEEECCEEEEECCCC
MTPYEVVVYLNEKGAKHGVGRIDIVENRLVGMKSRGCYETPGGTIINEALRAVEQLVLDK
CCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
TSFEFREELGIKASHLVYDGRWFTPLCKSILAASEELAKDVNGEVVIKLYKGHATVIQKR
CCHHHHHHHCCEEEEEEECCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEC
SDNSLYSEEFATFGADEVYDQSHAEGFIRLYSLSSRIRALNSK
CCCCHHHHHHHHCCCHHHHCCHHHCCEEEEEHHHHHHHHHCCC
>Mature Secondary Structure 
SKKVEVNKVVVAYSGGLDTSVIIPWLKENYNCEVIAFVADVGQGEEELEGIEAKAIASG
CCEEEEEEEEEEEECCCCCEEEEEHHHCCCCEEEEEEEECCCCCHHHHCCCCHHHHHCC
ATECYVVDLKEEMVSEYIFPTLKTGALYEGKYLLGTSMARPIIAKAQVEVARNVGADALC
CCEEEEEECHHHHHHHHHCCCCCCCCEECCCEEECCHHCCCHHHHHHHHHHHCCCHHHHE
HGCTGKGNDQIRFEGAFAALAPDLHVIAPWREWDLVSREECLDYLAERNIPCTASLTKIY
ECCCCCCCCEEEEECEEEECCCCCEEECCCHHCCCCCHHHHHHHHHHCCCCCCHHHHHHH
SRDANAWHISTEGGVLEETWNAPNDDCWAWTVDPEQAPNESETISLKVEKGAVVAVDGKA
CCCCCEEEEECCCCEEHHCCCCCCCCEEEEEECHHHCCCCCCEEEEEEECCEEEEECCCC
MTPYEVVVYLNEKGAKHGVGRIDIVENRLVGMKSRGCYETPGGTIINEALRAVEQLVLDK
CCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
TSFEFREELGIKASHLVYDGRWFTPLCKSILAASEELAKDVNGEVVIKLYKGHATVIQKR
CCHHHHHHHCCEEEEEEECCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEC
SDNSLYSEEFATFGADEVYDQSHAEGFIRLYSLSSRIRALNSK
CCCCHHHHHHHHCCCHHHHCCHHHCCEEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA