| Definition | Vibrio fischeri ES114 chromosome I, complete genome. |
|---|---|
| Accession | NC_006840 |
| Length | 2,897,536 |
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The map label for this gene is mrcA [H]
Identifier: 59712905
GI number: 59712905
Start: 2580202
End: 2582700
Strand: Direct
Name: mrcA [H]
Synonym: VF_2298
Alternate gene names: 59712905
Gene position: 2580202-2582700 (Clockwise)
Preceding gene: 59712887
Following gene: 59712907
Centisome position: 89.05
GC content: 41.14
Gene sequence:
>2499_bases GTGAAGTTCATAAAGGCACTACTAATAGTTACATTGGTTTGCATAATTCTTGGGGTCACAACAATTTTTGGTTTCTACCA ATATGTGAAGCCAGAATTACCAGATGTGGCAACATTAAAAGATGTTCAACTGCAAACACCAATGCAGGTATATAGCCGCG ATGGTAAGTTAATCGCACAGTTTGGTGAAAAGCGCCGTATTCCATTAAAACTGGAAGAAATGCCGCCTCATCTACTTGAA GCCGTCATTGCCACAGAAGATAGCCGCTTTTATAGCCACTATGGTTTTGATCCTATCGGTATCACTCGTGCCGCCTTTGC TGTATTAGCTTCTGGCTCTGCAAAGCAAGGGGCGAGTACCATTACCCAACAACTTGCGCGTAACTTCTTTTTATCTAATG AAAAGAAAATCATGCGTAAAATTAAAGAGATCTTCATTGCCGTTCACATTGAACAGCTATTAACAAAGCAAGAGATCTTA GAACTCTACTTAAATAAAATTTACTTAGGTTATCGTTCTTATGGTGTTGGTGCTGCTGCTCATGTTTACTTTGGTAAAGA AGTGAGTCAACTGACCTTAGGTGAAGTTGCTATTATTGCAGGTTTACCAAAAGCCCCATCAACAATGAACCCAATTTATT CTGTTGATCGTGCAACAACACGTCGTAATGTTGTTCTTGCTCGTATGCTTGATGAAGGCTACATAACAAAACAAGAATTT GATGATGCAAAGGCCGAGGTGGTTATCTCTAAATACCACGGTGCAGAAATCGAATTACAAGCACCCTATATTGCTGAAAT TGCTCGTGCTTGGATGGTAAACAAATACGGTGAAGAAGCGGCTTATACTTCGGGTATGAACATCTACACAACCGTAGATT CAAAAATGCAAGCGGCTGCTAATAAGGCATCAATTGATAACCTATTAGCTTATGACGAGCGTCATGGTTACCGTGGTGCG GTTAAAACCGTATGGGAACCAAAAGCGACACCTCTTGATGAAGAAGCAATTACGAAGCACCTTAAAAACGAACCTTCATA CGGTGAGTTAATGCCTGCTGTTGTTACTTCAATCAAAGGAAAAACAGCAACGGTTGATATTAAAAATAATGGCGTCGCAA CTATTCCTTGGGATGGTTTAAAGTGGGCTCGTCGCTTTAAAACAGACAAGCGTCAAGGACCTGCGCCACGTCGTGCTTCT GATATCTTAGCGGTTGGTGAACAAATTTATGTTCGTCCATTAAGCCAAGAAACCAAAGATGATGTAACAACAACCGTATG GAAGCTAAGCCAAGTACCTGCAGCGAACACAGCATTTGTTGCTATGGACCCAAGAGATGGTGCAATCACCTCTTTAGTTG GTGGTTTTAACTTTGTTCATAACAAGTTTAACCGTGCAACTCAATCTGTTCGTCAGGTCGGTTCAAGTATCAAGCCATTC ATTTATTCAGCGGCACTTTACCACGGTAAAACATTAGCTAGTTTAATCAACGATGCGCCAATTAATACATGGGATGAGAG TCAAGGTACAGCATGGAGACCTAAAAACTCACCACCAACTTATGTTGGCCCTGCTCGTTTACGCATTGGTTTAGCTCAAT CTAAAAACGTAATGGCGGTTCGTGTTTTACGTGATGTAGGTTTAGATGAAACTATCGATTACCTAACACGTTTTGGTTTT AAGAAAGATGAACTTCCACGCTCTGAAACCATTGCTCTTGGAGCTGGTAGTTTAACACCAGTTCAAATGGCACAAGGCTT TGCTGTTTTTGCTAACAATGGTTACTACGTAGAACCTTACTATATTGACCATATTACTGGCCCGTTCGGTGATGAAGTTT ACAAAGCGACACCAAAAACCATTTGTCAGCGTGATTGTTCTCATCAAAATGATGAAGATTCACCTTATGCGAAAAAAGTA ATTTCTGCACAAAACGCATTCTTAACTAAACAAATGATGTACAGCAACATTTGGGGTGGTGGTAGTTGGCGTAAAGGTAC AGGTTGGAATGGTACAGGCTGGCGTGCACAAGTACTAAAACGTCGTGATATCGGTGGTAAAACAGGTACAACTAACGACT CTGTTGATGCTTGGTACAATGGCTATGGCCCGAATGTAGTAGCAACAGCATGGGTTGGTTTTGATAACCCGTCGCACCGA CTAGGTTACACCACGAAAAACGACAACATGACCAAAGAAGAAATGTCTTTTGGTGGTGAAGCTGGTGGTAAAACAGCTAT TTACGCTTGGGTTAATTTCATGAAGGTCGCACTAGAAGGTGTTCCTGAAGAGAAGCTACAACTTCCGGCAAACATCATTA AGGTGAAGATAGATCGCGAAACTGGTCTACTAACCAACAAAAATGATGAAACAAGTATGTGGGAATATTTTGCTGGTGGA ACTCAACCAAAAGAATATGTAGAACAAGATTTCCAAGACACTATTTATTCATCACACGATGCAGATGGTGACGGAATGGA AGACGAAAGCCTGTTCTAA
Upstream 100 bases:
>100_bases TTAAGCGTTTATTTGCGTTATTTTTTGTTGTGTTATATTTATACTACTCAGTTATGGGTCAGAAACGATAATACAGATTA AAGTAAACGGGAAATATCAG
Downstream 100 bases:
>100_bases TCCCTTAAAATCACTTTAATAAAAAGGCACGCTACATATCTAGCGTGCCTTTTTTATAAGCTTAATTTAGCTACGAATAA CATTAGACATTTTATTCTCA
Product: fused penicillin-binding protein 1a: murein transglycosylase/murein transpeptidase
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 832; Mature: 832
Protein sequence:
>832_residues MKFIKALLIVTLVCIILGVTTIFGFYQYVKPELPDVATLKDVQLQTPMQVYSRDGKLIAQFGEKRRIPLKLEEMPPHLLE AVIATEDSRFYSHYGFDPIGITRAAFAVLASGSAKQGASTITQQLARNFFLSNEKKIMRKIKEIFIAVHIEQLLTKQEIL ELYLNKIYLGYRSYGVGAAAHVYFGKEVSQLTLGEVAIIAGLPKAPSTMNPIYSVDRATTRRNVVLARMLDEGYITKQEF DDAKAEVVISKYHGAEIELQAPYIAEIARAWMVNKYGEEAAYTSGMNIYTTVDSKMQAAANKASIDNLLAYDERHGYRGA VKTVWEPKATPLDEEAITKHLKNEPSYGELMPAVVTSIKGKTATVDIKNNGVATIPWDGLKWARRFKTDKRQGPAPRRAS DILAVGEQIYVRPLSQETKDDVTTTVWKLSQVPAANTAFVAMDPRDGAITSLVGGFNFVHNKFNRATQSVRQVGSSIKPF IYSAALYHGKTLASLINDAPINTWDESQGTAWRPKNSPPTYVGPARLRIGLAQSKNVMAVRVLRDVGLDETIDYLTRFGF KKDELPRSETIALGAGSLTPVQMAQGFAVFANNGYYVEPYYIDHITGPFGDEVYKATPKTICQRDCSHQNDEDSPYAKKV ISAQNAFLTKQMMYSNIWGGGSWRKGTGWNGTGWRAQVLKRRDIGGKTGTTNDSVDAWYNGYGPNVVATAWVGFDNPSHR LGYTTKNDNMTKEEMSFGGEAGGKTAIYAWVNFMKVALEGVPEEKLQLPANIIKVKIDRETGLLTNKNDETSMWEYFAGG TQPKEYVEQDFQDTIYSSHDADGDGMEDESLF
Sequences:
>Translated_832_residues MKFIKALLIVTLVCIILGVTTIFGFYQYVKPELPDVATLKDVQLQTPMQVYSRDGKLIAQFGEKRRIPLKLEEMPPHLLE AVIATEDSRFYSHYGFDPIGITRAAFAVLASGSAKQGASTITQQLARNFFLSNEKKIMRKIKEIFIAVHIEQLLTKQEIL ELYLNKIYLGYRSYGVGAAAHVYFGKEVSQLTLGEVAIIAGLPKAPSTMNPIYSVDRATTRRNVVLARMLDEGYITKQEF DDAKAEVVISKYHGAEIELQAPYIAEIARAWMVNKYGEEAAYTSGMNIYTTVDSKMQAAANKASIDNLLAYDERHGYRGA VKTVWEPKATPLDEEAITKHLKNEPSYGELMPAVVTSIKGKTATVDIKNNGVATIPWDGLKWARRFKTDKRQGPAPRRAS DILAVGEQIYVRPLSQETKDDVTTTVWKLSQVPAANTAFVAMDPRDGAITSLVGGFNFVHNKFNRATQSVRQVGSSIKPF IYSAALYHGKTLASLINDAPINTWDESQGTAWRPKNSPPTYVGPARLRIGLAQSKNVMAVRVLRDVGLDETIDYLTRFGF KKDELPRSETIALGAGSLTPVQMAQGFAVFANNGYYVEPYYIDHITGPFGDEVYKATPKTICQRDCSHQNDEDSPYAKKV ISAQNAFLTKQMMYSNIWGGGSWRKGTGWNGTGWRAQVLKRRDIGGKTGTTNDSVDAWYNGYGPNVVATAWVGFDNPSHR LGYTTKNDNMTKEEMSFGGEAGGKTAIYAWVNFMKVALEGVPEEKLQLPANIIKVKIDRETGLLTNKNDETSMWEYFAGG TQPKEYVEQDFQDTIYSSHDADGDGMEDESLF >Mature_832_residues MKFIKALLIVTLVCIILGVTTIFGFYQYVKPELPDVATLKDVQLQTPMQVYSRDGKLIAQFGEKRRIPLKLEEMPPHLLE AVIATEDSRFYSHYGFDPIGITRAAFAVLASGSAKQGASTITQQLARNFFLSNEKKIMRKIKEIFIAVHIEQLLTKQEIL ELYLNKIYLGYRSYGVGAAAHVYFGKEVSQLTLGEVAIIAGLPKAPSTMNPIYSVDRATTRRNVVLARMLDEGYITKQEF DDAKAEVVISKYHGAEIELQAPYIAEIARAWMVNKYGEEAAYTSGMNIYTTVDSKMQAAANKASIDNLLAYDERHGYRGA VKTVWEPKATPLDEEAITKHLKNEPSYGELMPAVVTSIKGKTATVDIKNNGVATIPWDGLKWARRFKTDKRQGPAPRRAS DILAVGEQIYVRPLSQETKDDVTTTVWKLSQVPAANTAFVAMDPRDGAITSLVGGFNFVHNKFNRATQSVRQVGSSIKPF IYSAALYHGKTLASLINDAPINTWDESQGTAWRPKNSPPTYVGPARLRIGLAQSKNVMAVRVLRDVGLDETIDYLTRFGF KKDELPRSETIALGAGSLTPVQMAQGFAVFANNGYYVEPYYIDHITGPFGDEVYKATPKTICQRDCSHQNDEDSPYAKKV ISAQNAFLTKQMMYSNIWGGGSWRKGTGWNGTGWRAQVLKRRDIGGKTGTTNDSVDAWYNGYGPNVVATAWVGFDNPSHR LGYTTKNDNMTKEEMSFGGEAGGKTAIYAWVNFMKVALEGVPEEKLQLPANIIKVKIDRETGLLTNKNDETSMWEYFAGG TQPKEYVEQDFQDTIYSSHDADGDGMEDESLF
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=866, Percent_Identity=50.9237875288684, Blast_Score=876, Evalue=0.0, Organism=Escherichia coli, GI1786343, Length=287, Percent_Identity=32.404181184669, Blast_Score=139, Evalue=6e-34, Organism=Escherichia coli, GI1788867, Length=251, Percent_Identity=33.0677290836653, Blast_Score=133, Evalue=4e-32, Organism=Escherichia coli, GI1789601, Length=176, Percent_Identity=32.3863636363636, Blast_Score=87, Evalue=3e-18,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.- [C]
Molecular weight: Translated: 92498; Mature: 92498
Theoretical pI: Translated: 8.28; Mature: 8.28
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKFIKALLIVTLVCIILGVTTIFGFYQYVKPELPDVATLKDVQLQTPMQVYSRDGKLIAQ CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHH FGEKRRIPLKLEEMPPHLLEAVIATEDSRFYSHYGFDPIGITRAAFAVLASGSAKQGAST HCCCCCCCEEHHCCCHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHH ITQQLARNFFLSNEKKIMRKIKEIFIAVHIEQLLTKQEILELYLNKIYLGYRSYGVGAAA HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE HVYFGKEVSQLTLGEVAIIAGLPKAPSTMNPIYSVDRATTRRNVVLARMLDEGYITKQEF EEEECCHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH DDAKAEVVISKYHGAEIELQAPYIAEIARAWMVNKYGEEAAYTSGMNIYTTVDSKMQAAA CCCHHEEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCHHHHHHH NKASIDNLLAYDERHGYRGAVKTVWEPKATPLDEEAITKHLKNEPSYGELMPAVVTSIKG HHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC KTATVDIKNNGVATIPWDGLKWARRFKTDKRQGPAPRRASDILAVGEQIYVRPLSQETKD CEEEEEECCCCEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHEEEECCCCHHH DVTTTVWKLSQVPAANTAFVAMDPRDGAITSLVGGFNFVHNKFNRATQSVRQVGSSIKPF HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH IYSAALYHGKTLASLINDAPINTWDESQGTAWRPKNSPPTYVGPARLRIGLAQSKNVMAV HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEECCCCCHHHH RVLRDVGLDETIDYLTRFGFKKDELPRSETIALGAGSLTPVQMAQGFAVFANNGYYVEPY HHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHCCEEEEECCCEEECCE YIDHITGPFGDEVYKATPKTICQRDCSHQNDEDSPYAKKVISAQNAFLTKQMMYSNIWGG EEECCCCCCCCHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC GSWRKGTGWNGTGWRAQVLKRRDIGGKTGTTNDSVDAWYNGYGPNVVATAWVGFDNPSHR CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCC LGYTTKNDNMTKEEMSFGGEAGGKTAIYAWVNFMKVALEGVPEEKLQLPANIIKVKIDRE CCEEECCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHCCCEEEEEEEECC TGLLTNKNDETSMWEYFAGGTQPKEYVEQDFQDTIYSSHDADGDGMEDESLF CCCEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC >Mature Secondary Structure MKFIKALLIVTLVCIILGVTTIFGFYQYVKPELPDVATLKDVQLQTPMQVYSRDGKLIAQ CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHH FGEKRRIPLKLEEMPPHLLEAVIATEDSRFYSHYGFDPIGITRAAFAVLASGSAKQGAST HCCCCCCCEEHHCCCHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHH ITQQLARNFFLSNEKKIMRKIKEIFIAVHIEQLLTKQEILELYLNKIYLGYRSYGVGAAA HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE HVYFGKEVSQLTLGEVAIIAGLPKAPSTMNPIYSVDRATTRRNVVLARMLDEGYITKQEF EEEECCHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH DDAKAEVVISKYHGAEIELQAPYIAEIARAWMVNKYGEEAAYTSGMNIYTTVDSKMQAAA CCCHHEEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCHHHHHHH NKASIDNLLAYDERHGYRGAVKTVWEPKATPLDEEAITKHLKNEPSYGELMPAVVTSIKG HHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC KTATVDIKNNGVATIPWDGLKWARRFKTDKRQGPAPRRASDILAVGEQIYVRPLSQETKD CEEEEEECCCCEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHEEEECCCCHHH DVTTTVWKLSQVPAANTAFVAMDPRDGAITSLVGGFNFVHNKFNRATQSVRQVGSSIKPF HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH IYSAALYHGKTLASLINDAPINTWDESQGTAWRPKNSPPTYVGPARLRIGLAQSKNVMAV HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEECCCCCHHHH RVLRDVGLDETIDYLTRFGFKKDELPRSETIALGAGSLTPVQMAQGFAVFANNGYYVEPY HHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHCCEEEEECCCEEECCE YIDHITGPFGDEVYKATPKTICQRDCSHQNDEDSPYAKKVISAQNAFLTKQMMYSNIWGG EEECCCCCCCCHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC GSWRKGTGWNGTGWRAQVLKRRDIGGKTGTTNDSVDAWYNGYGPNVVATAWVGFDNPSHR CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCC LGYTTKNDNMTKEEMSFGGEAGGKTAIYAWVNFMKVALEGVPEEKLQLPANIIKVKIDRE CCEEECCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHCCCEEEEEEEECC TGLLTNKNDETSMWEYFAGGTQPKEYVEQDFQDTIYSSHDADGDGMEDESLF CCCEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 10952301 [H]