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The map label for this gene is leuO [H]

Identifier: 59712872

GI number: 59712872

Start: 2543092

End: 2544063

Strand: Reverse

Name: leuO [H]

Synonym: VF_2265

Alternate gene names: 59712872

Gene position: 2544063-2543092 (Counterclockwise)

Preceding gene: 59712873

Following gene: 59712871

Centisome position: 87.8

GC content: 38.89

Gene sequence:

>972_bases
ATGAATACGCCTGAAACTTCTTTATCTTCTGTTGCACGTACAGAACAAATACTACGTGGTGTTGATCTTAACTTATTGAC
TGTTTTTGATGCGGTAATGCAAGAACAAAATATAACAAGAGCAGCACAAAACTTAGGTATGTCACAGCCAGCAGTAAGTA
ATGCGGTATCACGTTTAAAATTAATGTTTAATGATGAATTATTCATTCGTCATGGCCGTGGTATTCAACCGACACAACGT
GCTCGTCAGCTATTTGCACCGATGCGTCAGGCGCTTCAATTGGTTAAAAACGAATTACCAAGCTCAACATTCTCACCTGA
AACATCGATGAGACAATTTAAGCTATCTATTTGCAGCCCATCAGACATTCGTTTTGCACCACGCATCATGAAAGATATTC
AAAATAAAGCGCCGCATATTAGCTTAAAGCTTTCTGGTGAACATGAACCAGACATTGCTCAAAAGCTGCGTTATCAAGAA
GTGGATTTTGTTATCGATTACGTTCGATTCGACGAACCTGGTTTTATGAGTACAGAGCTATTTACTGATGAGTTAGTTGT
GGTTGCTTCTGTTAATCACCCTCGACTTCAGAACGGTATCAACAAAGAACAATTAGCAAGCGAAAAACATGCGGTTCCAG
CTCGTATCGCAAATACTAAAGGTTTTGCAGATCACCTGTATGTGGATTCTTTGTATGAGCAAGCTTATAAAGGCGCAAGC
ATTAGTAATATTTTGTATGTTGTTTCTCAATCAGAACTGATCACTGTAGCGCCAAAATGGTTAGTGAACTCAATGCCAAA
TCGTGATCAATTAATGGTGTTACCACTGCCAATTGAGTCAAAAGGCATTAGTGGCTATTTAAGCTGGCACGACTCTTACC
AAAAAGACAAAGGTCATATTTGGATGAGAGATCAATTGATGCTTATTTGTGGTGAAGAAGTGGTTGCATCAAGCAAAGGC
ATGATGTTTTAA

Upstream 100 bases:

>100_bases
GAAGTTCGATTAACATACGTCTGTTCAATATCTATAACAAAAATAAAATGTATGTAATCGTTTTCTTTATGATAATGATT
AATTGCTGAGAGAGTAATAC

Downstream 100 bases:

>100_bases
GCCTTTAAAATAGACACAATAATGGAAAAAGGTTGCGTATTTTACGTAACCTTTTTTTTAAACACATTATTCGATATAAC
GAAAATGTAGATTATTAATA

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNTPETSLSSVARTEQILRGVDLNLLTVFDAVMQEQNITRAAQNLGMSQPAVSNAVSRLKLMFNDELFIRHGRGIQPTQR
ARQLFAPMRQALQLVKNELPSSTFSPETSMRQFKLSICSPSDIRFAPRIMKDIQNKAPHISLKLSGEHEPDIAQKLRYQE
VDFVIDYVRFDEPGFMSTELFTDELVVVASVNHPRLQNGINKEQLASEKHAVPARIANTKGFADHLYVDSLYEQAYKGAS
ISNILYVVSQSELITVAPKWLVNSMPNRDQLMVLPLPIESKGISGYLSWHDSYQKDKGHIWMRDQLMLICGEEVVASSKG
MMF

Sequences:

>Translated_323_residues
MNTPETSLSSVARTEQILRGVDLNLLTVFDAVMQEQNITRAAQNLGMSQPAVSNAVSRLKLMFNDELFIRHGRGIQPTQR
ARQLFAPMRQALQLVKNELPSSTFSPETSMRQFKLSICSPSDIRFAPRIMKDIQNKAPHISLKLSGEHEPDIAQKLRYQE
VDFVIDYVRFDEPGFMSTELFTDELVVVASVNHPRLQNGINKEQLASEKHAVPARIANTKGFADHLYVDSLYEQAYKGAS
ISNILYVVSQSELITVAPKWLVNSMPNRDQLMVLPLPIESKGISGYLSWHDSYQKDKGHIWMRDQLMLICGEEVVASSKG
MMF
>Mature_323_residues
MNTPETSLSSVARTEQILRGVDLNLLTVFDAVMQEQNITRAAQNLGMSQPAVSNAVSRLKLMFNDELFIRHGRGIQPTQR
ARQLFAPMRQALQLVKNELPSSTFSPETSMRQFKLSICSPSDIRFAPRIMKDIQNKAPHISLKLSGEHEPDIAQKLRYQE
VDFVIDYVRFDEPGFMSTELFTDELVVVASVNHPRLQNGINKEQLASEKHAVPARIANTKGFADHLYVDSLYEQAYKGAS
ISNILYVVSQSELITVAPKWLVNSMPNRDQLMVLPLPIESKGISGYLSWHDSYQKDKGHIWMRDQLMLICGEEVVASSKG
MMF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=295, Percent_Identity=51.5254237288136, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1786820, Length=294, Percent_Identity=25.8503401360544, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081766, Length=264, Percent_Identity=24.2424242424242, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36498; Mature: 36498

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPETSLSSVARTEQILRGVDLNLLTVFDAVMQEQNITRAAQNLGMSQPAVSNAVSRLK
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LMFNDELFIRHGRGIQPTQRARQLFAPMRQALQLVKNELPSSTFSPETSMRQFKLSICSP
HHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEEEECCC
SDIRFAPRIMKDIQNKAPHISLKLSGEHEPDIAQKLRYQEVDFVIDYVRFDEPGFMSTEL
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCHHH
FTDELVVVASVNHPRLQNGINKEQLASEKHAVPARIANTKGFADHLYVDSLYEQAYKGAS
HCCCEEEEEECCCCHHHCCCCHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCC
ISNILYVVSQSELITVAPKWLVNSMPNRDQLMVLPLPIESKGISGYLSWHDSYQKDKGHI
HHHEEEEEECCCEEEECHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCE
WMRDQLMLICGEEVVASSKGMMF
EEECCEEEEECHHHHHCCCCCCC
>Mature Secondary Structure
MNTPETSLSSVARTEQILRGVDLNLLTVFDAVMQEQNITRAAQNLGMSQPAVSNAVSRLK
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LMFNDELFIRHGRGIQPTQRARQLFAPMRQALQLVKNELPSSTFSPETSMRQFKLSICSP
HHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEEEECCC
SDIRFAPRIMKDIQNKAPHISLKLSGEHEPDIAQKLRYQEVDFVIDYVRFDEPGFMSTEL
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCHHH
FTDELVVVASVNHPRLQNGINKEQLASEKHAVPARIANTKGFADHLYVDSLYEQAYKGAS
HCCCEEEEEECCCCHHHCCCCHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCC
ISNILYVVSQSELITVAPKWLVNSMPNRDQLMVLPLPIESKGISGYLSWHDSYQKDKGHI
HHHEEEEEECCCEEEECHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCE
WMRDQLMLICGEEVVASSKGMMF
EEECCEEEEECHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]