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The map label for this gene is murE [H]

Identifier: 59712813

GI number: 59712813

Start: 2478660

End: 2480141

Strand: Reverse

Name: murE [H]

Synonym: VF_2206

Alternate gene names: 59712813

Gene position: 2480141-2478660 (Counterclockwise)

Preceding gene: 59712814

Following gene: 59712812

Centisome position: 85.59

GC content: 40.55

Gene sequence:

>1482_bases
ATGATGCAGTTATCTGAATTACTGTTGCCTTGGTGCGATTTAAATCAATCTGTGGCGATTTCATCTATGACATTGGACAG
TCGCCAAGTTTCTCCAGGCTGTTTATTTGTAGCAATTAAAGGCCATGCAGTGGATGGCCGTCAATTTATTTCGTCGGCAA
TTGAGCAAGGCGCTGTAGCAGTTATTGCTGATGCTGATAAAGAGAATATTGCTGGAACGGTAAAGGTTATCGATAACGCT
ATTGTTGTTTACATTGATTCTCTTTATACGCAGTTAAGTGCGATTGCAGGAGCTTTTTATCAAGCGGATAAGAGAAACAT
GCAACTCGTCGGTATCACAGGTACTAATGGTAAAACGACGATTTCTCAGATCATTGCACAATGGACGACATTATTAGAAC
AACCCGCTGCCGTAATGGGAACAACGGGTAATGGCTTACTGTCGGATCTTAAGCCAGCTTTGAACACAACTGGCAGTCCA
ATTGAAATCCAACAAACACTGACAGAACTTGAGAATATCGGAGCTAAACTGACGGCATTAGAAGTGTCGTCTCATGGTTT
GGTTCAAGGGCGGGTTAATGCGTTATCTTTTGATGCTGCCGTATTTACTAACTTAAGCCGTGATCATCTTGATTACCATG
GTGATATGGCAAATTATGCTGAAGCTAAAAAGTTACTTTTTACTTCTCATCGTCCAAAAAACTCAATCATCAATGGGGAT
GATCAAGTAGGTTGCCAATGGCTAAAAGAGATGCCTCATGCTGTCGCGGTTTCTTTAAATGAAGAAAACATCTCTTTTCA
TTCCGGTAAGAAAGTATTTGCGACATCAGTTGTTTATTCAACCGAAGGCATAAAGCTTTCAATCTCTTCTGATTGGGGGA
ATGTTGCTTTTTCAGTGCCTTTAATTGGGGCATTTAATGCAAGTAATATTCTTGTTGCTTTCGCGGCACTTCTTTCTTTA
GGTTATGACATCCAAGCTTTAGCTCAAACGGCGTCTCAGCTGAATGCAGTTATTGGTCGAATGGAACTGTTTGCTACAGA
TAATAAAGCAAAAGTGGTGGTAGATTACGCGCATACACCTGACGCTTTAGATAAAGCATTGCAAGCATTACGTGTTCATT
GTGAAGGTAAGTTGTGGTGTATTTTCGGCTGTGGTGGCGATAGAGATACAGGCAAACGACCAATAATGGCAAGCATTGTT
GAGCAACAAGCGGATGCGATTATTTTAAGTGATGATAACCCTCGCTCAGAAGAACCAAGCTTGATTACGGATGATATGTT
AGTTGGATTAAAAAACGCCGAATCTGCACATGTTATTCATGATCGTTTTGAAGCTTGTCAGTTTGCATTATCTCAAGCAA
ATGAGAACGATATTATTTTACTTGCCGGTAAAGGGCATGAAGATTATCAAGTGTTAGCGGATAAGACCGTTCATTACTCT
GATCGTGAAACCGCACAAACATTATTAGGGCTACAATCATGA

Upstream 100 bases:

>100_bases
CCTATCTTTGGTCAGGTCATGAAAGATGCGTTGCAAATTTTAAACGTAGCGCCAGATGAAAGTACCGTAAAACATATCGA
TGTATTGAATTAGGAAAGTG

Downstream 100 bases:

>100_bases
TAACTATGTTACTTAGCGAAATAGCAAAAGCGTGTGATGGGCGTTTGGTTGGGGCTGATTGTCAAATATCGTCAGTATCA
ACAGACAGTCGACACATTGA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso- diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MMQLSELLLPWCDLNQSVAISSMTLDSRQVSPGCLFVAIKGHAVDGRQFISSAIEQGAVAVIADADKENIAGTVKVIDNA
IVVYIDSLYTQLSAIAGAFYQADKRNMQLVGITGTNGKTTISQIIAQWTTLLEQPAAVMGTTGNGLLSDLKPALNTTGSP
IEIQQTLTELENIGAKLTALEVSSHGLVQGRVNALSFDAAVFTNLSRDHLDYHGDMANYAEAKKLLFTSHRPKNSIINGD
DQVGCQWLKEMPHAVAVSLNEENISFHSGKKVFATSVVYSTEGIKLSISSDWGNVAFSVPLIGAFNASNILVAFAALLSL
GYDIQALAQTASQLNAVIGRMELFATDNKAKVVVDYAHTPDALDKALQALRVHCEGKLWCIFGCGGDRDTGKRPIMASIV
EQQADAIILSDDNPRSEEPSLITDDMLVGLKNAESAHVIHDRFEACQFALSQANENDIILLAGKGHEDYQVLADKTVHYS
DRETAQTLLGLQS

Sequences:

>Translated_493_residues
MMQLSELLLPWCDLNQSVAISSMTLDSRQVSPGCLFVAIKGHAVDGRQFISSAIEQGAVAVIADADKENIAGTVKVIDNA
IVVYIDSLYTQLSAIAGAFYQADKRNMQLVGITGTNGKTTISQIIAQWTTLLEQPAAVMGTTGNGLLSDLKPALNTTGSP
IEIQQTLTELENIGAKLTALEVSSHGLVQGRVNALSFDAAVFTNLSRDHLDYHGDMANYAEAKKLLFTSHRPKNSIINGD
DQVGCQWLKEMPHAVAVSLNEENISFHSGKKVFATSVVYSTEGIKLSISSDWGNVAFSVPLIGAFNASNILVAFAALLSL
GYDIQALAQTASQLNAVIGRMELFATDNKAKVVVDYAHTPDALDKALQALRVHCEGKLWCIFGCGGDRDTGKRPIMASIV
EQQADAIILSDDNPRSEEPSLITDDMLVGLKNAESAHVIHDRFEACQFALSQANENDIILLAGKGHEDYQVLADKTVHYS
DRETAQTLLGLQS
>Mature_493_residues
MMQLSELLLPWCDLNQSVAISSMTLDSRQVSPGCLFVAIKGHAVDGRQFISSAIEQGAVAVIADADKENIAGTVKVIDNA
IVVYIDSLYTQLSAIAGAFYQADKRNMQLVGITGTNGKTTISQIIAQWTTLLEQPAAVMGTTGNGLLSDLKPALNTTGSP
IEIQQTLTELENIGAKLTALEVSSHGLVQGRVNALSFDAAVFTNLSRDHLDYHGDMANYAEAKKLLFTSHRPKNSIINGD
DQVGCQWLKEMPHAVAVSLNEENISFHSGKKVFATSVVYSTEGIKLSISSDWGNVAFSVPLIGAFNASNILVAFAALLSL
GYDIQALAQTASQLNAVIGRMELFATDNKAKVVVDYAHTPDALDKALQALRVHCEGKLWCIFGCGGDRDTGKRPIMASIV
EQQADAIILSDDNPRSEEPSLITDDMLVGLKNAESAHVIHDRFEACQFALSQANENDIILLAGKGHEDYQVLADKTVHYS
DRETAQTLLGLQS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=489, Percent_Identity=52.760736196319, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1786274, Length=327, Percent_Identity=27.217125382263, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53049; Mature: 53049

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQLSELLLPWCDLNQSVAISSMTLDSRQVSPGCLFVAIKGHAVDGRQFISSAIEQGAVA
CCCHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEE
VIADADKENIAGTVKVIDNAIVVYIDSLYTQLSAIAGAFYQADKRNMQLVGITGTNGKTT
EEECCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHH
ISQIIAQWTTLLEQPAAVMGTTGNGLLSDLKPALNTTGSPIEIQQTLTELENIGAKLTAL
HHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCEEEEE
EVSSHGLVQGRVNALSFDAAVFTNLSRDHLDYHGDMANYAEAKKLLFTSHRPKNSIINGD
EECCCCEEECCCCEEEECHHHHHCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DQVGCQWLKEMPHAVAVSLNEENISFHSGKKVFATSVVYSTEGIKLSISSDWGNVAFSVP
HHHHHHHHHHCCCEEEEEECCCCCEECCCCEEEEEEEEEECCCEEEEEECCCCCEEEEEE
LIGAFNASNILVAFAALLSLGYDIQALAQTASQLNAVIGRMELFATDNKAKVVVDYAHTP
EEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCC
DALDKALQALRVHCEGKLWCIFGCGGDRDTGKRPIMASIVEQQADAIILSDDNPRSEEPS
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCC
LITDDMLVGLKNAESAHVIHDRFEACQFALSQANENDIILLAGKGHEDYQVLADKTVHYS
EECCHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHEECCEECCC
DRETAQTLLGLQS
CHHHHHHHHCCCC
>Mature Secondary Structure
MMQLSELLLPWCDLNQSVAISSMTLDSRQVSPGCLFVAIKGHAVDGRQFISSAIEQGAVA
CCCHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEE
VIADADKENIAGTVKVIDNAIVVYIDSLYTQLSAIAGAFYQADKRNMQLVGITGTNGKTT
EEECCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHH
ISQIIAQWTTLLEQPAAVMGTTGNGLLSDLKPALNTTGSPIEIQQTLTELENIGAKLTAL
HHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCEEEEE
EVSSHGLVQGRVNALSFDAAVFTNLSRDHLDYHGDMANYAEAKKLLFTSHRPKNSIINGD
EECCCCEEECCCCEEEECHHHHHCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DQVGCQWLKEMPHAVAVSLNEENISFHSGKKVFATSVVYSTEGIKLSISSDWGNVAFSVP
HHHHHHHHHHCCCEEEEEECCCCCEECCCCEEEEEEEEEECCCEEEEEECCCCCEEEEEE
LIGAFNASNILVAFAALLSLGYDIQALAQTASQLNAVIGRMELFATDNKAKVVVDYAHTP
EEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCC
DALDKALQALRVHCEGKLWCIFGCGGDRDTGKRPIMASIVEQQADAIILSDDNPRSEEPS
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCC
LITDDMLVGLKNAESAHVIHDRFEACQFALSQANENDIILLAGKGHEDYQVLADKTVHYS
EECCHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHEECCEECCC
DRETAQTLLGLQS
CHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA