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The map label for this gene is murF [H]

Identifier: 59712812

GI number: 59712812

Start: 2477305

End: 2478663

Strand: Reverse

Name: murF [H]

Synonym: VF_2205

Alternate gene names: 59712812

Gene position: 2478663-2477305 (Counterclockwise)

Preceding gene: 59712813

Following gene: 59712811

Centisome position: 85.54

GC content: 40.47

Gene sequence:

>1359_bases
ATGATAACTATGTTACTTAGCGAAATAGCAAAAGCGTGTGATGGGCGTTTGGTTGGGGCTGATTGTCAAATATCGTCAGT
ATCAACAGACAGTCGACACATTGATGAGGAAGGGCTTTTTGTTGCTCTTATCGGTGAACGCTTTGATGCCCACGATTTTA
TACCAGCAGTACAAGAGCAAGGAGCAACTGCACTTCTTGTATCAAAAGAAGTCGATACGACACTTCCTCAAGTAATTGTT
TCTGACACACAAAAGGCGTTAGGCTTAGTAGCGAAACATGTACATCAAGTTTGTCATACTTTTACATTTGCTTTAACTGG
TAGTTGTGGCAAAACCACAGTAAAAGAGATGCTCGCTTCTATTTTAACTCGTTCTGGCGAAGTGTTAGCAACCGCAGGTA
ACTTTAATAATGAGATCGGCGTTCCGTTAACGTTATTACGCTCTCAACCAAGTAATGATTTTGCCGTTATCGAATTGGGC
GCAAACCACCAAAATGAAATCTCATACACTACTCAGTTAGCACAACCTAATGTTGCACTGGTCAATAATATTGCAGCGGC
GCATTTAGAAGGTTTTGGCTCTATTGAGGGGGTTGCAAAAGCAAAAGGCGAGATTTTTGAAGGGCTACAAGAAAACGGTA
TTGCTATTTATAACCTAGATAGTAATGGCTTACCAAAATGGTCATCACTATTTGAAGGTAAACAAGTCGTTAGTTTTGCT
ATGGATGACTCTAGTGCTGACTTTTATGCATCAAATATTGAAGTAAATGCGATTGGTGAACCTAGTTTTCAATTAAATAC
GCCGAAAGGAAATATTCAAGTTTCTTTAGGGATTATTGGTAAGCATAACGTAGCGAATGCCGTTGCAGCTGCTGCGTTAG
CCATTCAAGCGCATGCATCATTAAATGACATTCAACAAGGTCTACAAGAGCTGGGTAGTGTGAAAGGGCGTGTTGCTGTC
GAAACATTAGCATCTGGATTGAAGGTTATCGATGACACTTATAACGCCAGTGTTCCTGCTATGAAAGCCGCAGTGGATTT
ATTGGGTGGATTTAAAGGTCAGCGTTGGCTTGTTTTAGGGAATATGGCCGAGCTAGGTGAAGAAAGCCTTGCACTTCATC
GTGAAGTTGGGGAATATGCTGCCCCATATCAATTTGAGCAAGTAGTGACATTTGGTGATGATACAAAAGTGATTAGCGAG
CTATGTAATGGCCGTCATTTTGAAACACGTTCTGATTTGAATGCATACTTACTTAAAGAATTACAATTAATTAAACATAA
TGAGGTTACCGTGTTAGTTAAAGGTGCCAACAGTTCACGAATGAGCGAAGTGGTTTCAGCTCTTAAGGAGTGTCAGTAA

Upstream 100 bases:

>100_bases
TACTTGCCGGTAAAGGGCATGAAGATTATCAAGTGTTAGCGGATAAGACCGTTCATTACTCTGATCGTGAAACCGCACAA
ACATTATTAGGGCTACAATC

Downstream 100 bases:

>100_bases
TGATTATTTGGTTATCCAATTTTCTAGAGCAATATTTTCCATTTTTTCGCTTATTTGAATATTTAAGTTTTAGAGCCATC
TTAAGCATTATCACGGCATT

Product: UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MITMLLSEIAKACDGRLVGADCQISSVSTDSRHIDEEGLFVALIGERFDAHDFIPAVQEQGATALLVSKEVDTTLPQVIV
SDTQKALGLVAKHVHQVCHTFTFALTGSCGKTTVKEMLASILTRSGEVLATAGNFNNEIGVPLTLLRSQPSNDFAVIELG
ANHQNEISYTTQLAQPNVALVNNIAAAHLEGFGSIEGVAKAKGEIFEGLQENGIAIYNLDSNGLPKWSSLFEGKQVVSFA
MDDSSADFYASNIEVNAIGEPSFQLNTPKGNIQVSLGIIGKHNVANAVAAAALAIQAHASLNDIQQGLQELGSVKGRVAV
ETLASGLKVIDDTYNASVPAMKAAVDLLGGFKGQRWLVLGNMAELGEESLALHREVGEYAAPYQFEQVVTFGDDTKVISE
LCNGRHFETRSDLNAYLLKELQLIKHNEVTVLVKGANSSRMSEVVSALKECQ

Sequences:

>Translated_452_residues
MITMLLSEIAKACDGRLVGADCQISSVSTDSRHIDEEGLFVALIGERFDAHDFIPAVQEQGATALLVSKEVDTTLPQVIV
SDTQKALGLVAKHVHQVCHTFTFALTGSCGKTTVKEMLASILTRSGEVLATAGNFNNEIGVPLTLLRSQPSNDFAVIELG
ANHQNEISYTTQLAQPNVALVNNIAAAHLEGFGSIEGVAKAKGEIFEGLQENGIAIYNLDSNGLPKWSSLFEGKQVVSFA
MDDSSADFYASNIEVNAIGEPSFQLNTPKGNIQVSLGIIGKHNVANAVAAAALAIQAHASLNDIQQGLQELGSVKGRVAV
ETLASGLKVIDDTYNASVPAMKAAVDLLGGFKGQRWLVLGNMAELGEESLALHREVGEYAAPYQFEQVVTFGDDTKVISE
LCNGRHFETRSDLNAYLLKELQLIKHNEVTVLVKGANSSRMSEVVSALKECQ
>Mature_452_residues
MITMLLSEIAKACDGRLVGADCQISSVSTDSRHIDEEGLFVALIGERFDAHDFIPAVQEQGATALLVSKEVDTTLPQVIV
SDTQKALGLVAKHVHQVCHTFTFALTGSCGKTTVKEMLASILTRSGEVLATAGNFNNEIGVPLTLLRSQPSNDFAVIELG
ANHQNEISYTTQLAQPNVALVNNIAAAHLEGFGSIEGVAKAKGEIFEGLQENGIAIYNLDSNGLPKWSSLFEGKQVVSFA
MDDSSADFYASNIEVNAIGEPSFQLNTPKGNIQVSLGIIGKHNVANAVAAAALAIQAHASLNDIQQGLQELGSVKGRVAV
ETLASGLKVIDDTYNASVPAMKAAVDLLGGFKGQRWLVLGNMAELGEESLALHREVGEYAAPYQFEQVVTFGDDTKVISE
LCNGRHFETRSDLNAYLLKELQLIKHNEVTVLVKGANSSRMSEVVSALKECQ

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=451, Percent_Identity=49.4456762749446, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48251; Mature: 48251

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMLLSEIAKACDGRLVGADCQISSVSTDSRHIDEEGLFVALIGERFDAHDFIPAVQEQ
CHHHHHHHHHHHHCCCEEECCCEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHC
GATALLVSKEVDTTLPQVIVSDTQKALGLVAKHVHQVCHTFTFALTGSCGKTTVKEMLAS
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
ILTRSGEVLATAGNFNNEIGVPLTLLRSQPSNDFAVIELGANHQNEISYTTQLAQPNVAL
HHHCCCCEEEECCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEHHCCCCHHH
VNNIAAAHLEGFGSIEGVAKAKGEIFEGLQENGIAIYNLDSNGLPKWSSLFEGKQVVSFA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHCCCCEEEEE
MDDSSADFYASNIEVNAIGEPSFQLNTPKGNIQVSLGIIGKHNVANAVAAAALAIQAHAS
ECCCCCCEEECCEEEEEECCCCEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCC
LNDIQQGLQELGSVKGRVAVETLASGLKVIDDTYNASVPAMKAAVDLLGGFKGQRWLVLG
HHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEC
NMAELGEESLALHREVGEYAAPYQFEQVVTFGDDTKVISELCNGRHFETRSDLNAYLLKE
CHHHHCHHHHHHHHHHHHHCCCCEEEHEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHH
LQLIKHNEVTVLVKGANSSRMSEVVSALKECQ
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MITMLLSEIAKACDGRLVGADCQISSVSTDSRHIDEEGLFVALIGERFDAHDFIPAVQEQ
CHHHHHHHHHHHHCCCEEECCCEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHC
GATALLVSKEVDTTLPQVIVSDTQKALGLVAKHVHQVCHTFTFALTGSCGKTTVKEMLAS
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
ILTRSGEVLATAGNFNNEIGVPLTLLRSQPSNDFAVIELGANHQNEISYTTQLAQPNVAL
HHHCCCCEEEECCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEHHCCCCHHH
VNNIAAAHLEGFGSIEGVAKAKGEIFEGLQENGIAIYNLDSNGLPKWSSLFEGKQVVSFA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHCCCCEEEEE
MDDSSADFYASNIEVNAIGEPSFQLNTPKGNIQVSLGIIGKHNVANAVAAAALAIQAHAS
ECCCCCCEEECCEEEEEECCCCEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCC
LNDIQQGLQELGSVKGRVAVETLASGLKVIDDTYNASVPAMKAAVDLLGGFKGQRWLVLG
HHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEC
NMAELGEESLALHREVGEYAAPYQFEQVVTFGDDTKVISELCNGRHFETRSDLNAYLLKE
CHHHHCHHHHHHHHHHHHHCCCCEEEHEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHH
LQLIKHNEVTVLVKGANSSRMSEVVSALKECQ
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]