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The map label for this gene is aceF [H]

Identifier: 59712786

GI number: 59712786

Start: 2448932

End: 2450818

Strand: Reverse

Name: aceF [H]

Synonym: VF_2179

Alternate gene names: 59712786

Gene position: 2450818-2448932 (Counterclockwise)

Preceding gene: 172087759

Following gene: 59712785

Centisome position: 84.58

GC content: 44.67

Gene sequence:

>1887_bases
ATGACAATCGAAATTAATGTACCTGACATCGGTGCAGATGAGGTTGAAGTAACTGAGATTCTAGTTAAGGTCGGTGACCG
AGTTGAAGAAGAGCAGTCACTGATCACTGTTGAAGGCGATAAAGCTTCTATGGAAGTTCCTGCGTCTCAAGCGGGTATCG
TTAAAGAGATCAAAATTGCTGAAGGTGATTCAGTAACAACTGGTTCTCTTATTATGATCTTTGAAGCAGAGGGTGCTGCA
GAAGCACCTGCTGCTCCAGCAGTTGAAGCAGCACCAGTTGCTGCTCCTGCTGCAGCATCAGTTGCAGAGCTTAAAGAAGT
TCACGTTCCTGATATCGGTGGTGATGAAGTTGAAGTAACTGAAATCATGGTTGCTGTTGGCGATACAGTAGAAGAAGAGC
AATCTCTTTTAACTGTTGAAGGCGACAAAGCGTCTATGGAAGTTCCAGCTCCATTCGCAGGTACGGTAAAAGAAATCAAA
ATTGCTTCTGGTGACTCAGTATCAACTGGTTCTCTAGTGATGATCTTTGAAGTTGCAGGTTCAGCACCAGCTGCACCAGA
AGTTGAAGCGGCAGCGCCAGTTGCAGCAGCTCCTGCGGCATCTGCAGAGAAAGAAGTGAACGTTCCAGATATCGGCGGTG
ATGAAGTTGAAGTAACTGAAATCATGGTTGCTGTTGGTGATACAGTAGAAGAAGAGCAATCTCTAATTACTGTTGAAGGC
GACAAAGCATCAATGGAAGTTCCTGCTCCATTTGCTGGTACAGTAAAAGAAATTAAGATTGCTGCAGGCGATAAAGTATC
GACTGGCTCTCTAATCATGACATTTGTTGTTGAAGGTACGGCACCAGCAGCTCCTCAAGCGGCAGCAGCCCCAGCTGCAC
AAGCAGCTCCTGCAGCGGCGCCAGTTGCAACAGCTTCTGCTCCTGCATCAACAGGTGAATTTGAAGCGAACAACGATTAC
GCTCATGCGTCTCCAGTTGTACGTCGTCTTGCTCGTGAATTTGGTGTAAACCTAGCGAAAGTTAAAGGTACTGGCCGTAA
GAACCGTATCCTTAAAGAAGACGTTCAAGGTTTCGTGAAAGATGCGCTTAAACGTCTTGAGTCTGGTGCAGCTGCATCAG
GTAAAGGTGGTGACGGTTCAGCTCTTGGTTTACTTCCTTGGCCAAAAGTTGATTTCAGCAAGTTTGGTGAAACAGAAGTT
CAGAAACTTTCTAAGATCAAGAAGATTTCTGGTGCAAACCTACACCGTAACTGGGTAATGATCCCTCACGTTACACAGTG
GGATAACGCAGACATCACTGAACTAGAAGCATTCCGTAAAGAGCAAAATGCAATTGAAGCTAAAAATGATACAGGCATGA
AGATCACGCCACTTGTATTCATTATGAAAGCGGTTGCTAAAGCACTTGAAGCGTTCCCAGCGTTTAACTCTTCTCTATCT
GAAGATGGCGAAAGCATCATTCTTAAGAAGTACGTAAACGTAGGTATCGCAGTTGATACGCCAAATGGCCTAGTTGTTCC
TGTATTTAAAGACGTGAACAAAAAAGGTATTTACGAGCTATCTGAAGAGCTAATGGCTGTTTCTAAGAAAGCACGTGCTG
GTAAACTAACAGCTTCTGATATGCAAGGCGGTTGTTTCACAATCTCAAGCCTTGGTGGTATCGGTGGTACTGCATTTACA
CCAATCGTAAATGCACCAGAAGTTGGTATCCTAGGTGTTTCTAAGTCTGAGATTAAACCTGTATGGAATGGCAAAGAGTT
CGCTCCGCGTCTACAACTTCCACTGTCTCTGTCTTATGACCACCGTGTTATCGATGGCGCAGAAGGTGCTCGTTTCATTA
CTTTCTTAAACAGTGCATTATCAGACATTCGTCGTCTAGTACTGTAA

Upstream 100 bases:

>100_bases
GAAATCTGTTGTTACAGAAGCAATTGCTAAATTCAATATCGATACAGAAAAAACTAACCCGCAATACGCATAAGACTTCC
ATTAATTAAGGAAACAAACA

Downstream 100 bases:

>100_bases
TTAGCGGTTATTGAAACAAACAATAAGGTGGCTAATAGGTCACCTTATTGCTTAATAAGCTTAAGGATTTATTCCTTACA
TTATATTTTTTAAAGCAATT

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MTIEINVPDIGADEVEVTEILVKVGDRVEEEQSLITVEGDKASMEVPASQAGIVKEIKIAEGDSVTTGSLIMIFEAEGAA
EAPAAPAVEAAPVAAPAAASVAELKEVHVPDIGGDEVEVTEIMVAVGDTVEEEQSLLTVEGDKASMEVPAPFAGTVKEIK
IASGDSVSTGSLVMIFEVAGSAPAAPEVEAAAPVAAAPAASAEKEVNVPDIGGDEVEVTEIMVAVGDTVEEEQSLITVEG
DKASMEVPAPFAGTVKEIKIAAGDKVSTGSLIMTFVVEGTAPAAPQAAAAPAAQAAPAAAPVATASAPASTGEFEANNDY
AHASPVVRRLAREFGVNLAKVKGTGRKNRILKEDVQGFVKDALKRLESGAAASGKGGDGSALGLLPWPKVDFSKFGETEV
QKLSKIKKISGANLHRNWVMIPHVTQWDNADITELEAFRKEQNAIEAKNDTGMKITPLVFIMKAVAKALEAFPAFNSSLS
EDGESIILKKYVNVGIAVDTPNGLVVPVFKDVNKKGIYELSEELMAVSKKARAGKLTASDMQGGCFTISSLGGIGGTAFT
PIVNAPEVGILGVSKSEIKPVWNGKEFAPRLQLPLSLSYDHRVIDGAEGARFITFLNSALSDIRRLVL

Sequences:

>Translated_628_residues
MTIEINVPDIGADEVEVTEILVKVGDRVEEEQSLITVEGDKASMEVPASQAGIVKEIKIAEGDSVTTGSLIMIFEAEGAA
EAPAAPAVEAAPVAAPAAASVAELKEVHVPDIGGDEVEVTEIMVAVGDTVEEEQSLLTVEGDKASMEVPAPFAGTVKEIK
IASGDSVSTGSLVMIFEVAGSAPAAPEVEAAAPVAAAPAASAEKEVNVPDIGGDEVEVTEIMVAVGDTVEEEQSLITVEG
DKASMEVPAPFAGTVKEIKIAAGDKVSTGSLIMTFVVEGTAPAAPQAAAAPAAQAAPAAAPVATASAPASTGEFEANNDY
AHASPVVRRLAREFGVNLAKVKGTGRKNRILKEDVQGFVKDALKRLESGAAASGKGGDGSALGLLPWPKVDFSKFGETEV
QKLSKIKKISGANLHRNWVMIPHVTQWDNADITELEAFRKEQNAIEAKNDTGMKITPLVFIMKAVAKALEAFPAFNSSLS
EDGESIILKKYVNVGIAVDTPNGLVVPVFKDVNKKGIYELSEELMAVSKKARAGKLTASDMQGGCFTISSLGGIGGTAFT
PIVNAPEVGILGVSKSEIKPVWNGKEFAPRLQLPLSLSYDHRVIDGAEGARFITFLNSALSDIRRLVL
>Mature_627_residues
TIEINVPDIGADEVEVTEILVKVGDRVEEEQSLITVEGDKASMEVPASQAGIVKEIKIAEGDSVTTGSLIMIFEAEGAAE
APAAPAVEAAPVAAPAAASVAELKEVHVPDIGGDEVEVTEIMVAVGDTVEEEQSLLTVEGDKASMEVPAPFAGTVKEIKI
ASGDSVSTGSLVMIFEVAGSAPAAPEVEAAAPVAAAPAASAEKEVNVPDIGGDEVEVTEIMVAVGDTVEEEQSLITVEGD
KASMEVPAPFAGTVKEIKIAAGDKVSTGSLIMTFVVEGTAPAAPQAAAAPAAQAAPAAAPVATASAPASTGEFEANNDYA
HASPVVRRLAREFGVNLAKVKGTGRKNRILKEDVQGFVKDALKRLESGAAASGKGGDGSALGLLPWPKVDFSKFGETEVQ
KLSKIKKISGANLHRNWVMIPHVTQWDNADITELEAFRKEQNAIEAKNDTGMKITPLVFIMKAVAKALEAFPAFNSSLSE
DGESIILKKYVNVGIAVDTPNGLVVPVFKDVNKKGIYELSEELMAVSKKARAGKLTASDMQGGCFTISSLGGIGGTAFTP
IVNAPEVGILGVSKSEIKPVWNGKEFAPRLQLPLSLSYDHRVIDGAEGARFITFLNSALSDIRRLVL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=31.1926605504587, Blast_Score=169, Evalue=9e-42,
Organism=Homo sapiens, GI31711992, Length=325, Percent_Identity=33.8461538461538, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=31.8965517241379, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI203098816, Length=230, Percent_Identity=31.7391304347826, Blast_Score=109, Evalue=1e-23,
Organism=Homo sapiens, GI203098753, Length=230, Percent_Identity=31.7391304347826, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=35.2564102564103, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1786305, Length=634, Percent_Identity=69.8738170347003, Blast_Score=796, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=406, Percent_Identity=30.2955665024631, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=30, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=431, Percent_Identity=30.1624129930394, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=209, Percent_Identity=36.8421052631579, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=29.4303797468354, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=445, Percent_Identity=27.1910112359551, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=354, Percent_Identity=29.3785310734463, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=31.651376146789, Blast_Score=167, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=32.579185520362, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=29.8701298701299, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI20129315, Length=213, Percent_Identity=31.924882629108, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 65266; Mature: 65135

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEINVPDIGADEVEVTEILVKVGDRVEEEQSLITVEGDKASMEVPASQAGIVKEIKIA
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCEEEEEEC
EGDSVTTGSLIMIFEAEGAAEAPAAPAVEAAPVAAPAAASVAELKEVHVPDIGGDEVEVT
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEHE
EIMVAVGDTVEEEQSLLTVEGDKASMEVPAPFAGTVKEIKIASGDSVSTGSLVMIFEVAG
EEEEHHCCCHHCCCCEEEEECCCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEECC
SAPAAPEVEAAAPVAAAPAASAEKEVNVPDIGGDEVEVTEIMVAVGDTVEEEQSLITVEG
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEHEEEEEHHCCCCCCCCCEEEEEC
DKASMEVPAPFAGTVKEIKIAAGDKVSTGSLIMTFVVEGTAPAAPQAAAAPAAQAAPAAA
CCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCHHCCCCHHCCCCCC
PVATASAPASTGEFEANNDYAHASPVVRRLAREFGVNLAKVKGTGRKNRILKEDVQGFVK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHH
DALKRLESGAAASGKGGDGSALGLLPWPKVDFSKFGETEVQKLSKIKKISGANLHRNWVM
HHHHHHHCCCCCCCCCCCCCEEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCEEE
IPHVTQWDNADITELEAFRKEQNAIEAKNDTGMKITPLVFIMKAVAKALEAFPAFNSSLS
ECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHH
EDGESIILKKYVNVGIAVDTPNGLVVPVFKDVNKKGIYELSEELMAVSKKARAGKLTASD
CCCHHEEEEECCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
MQGGCFTISSLGGIGGTAFTPIVNAPEVGILGVSKSEIKPVWNGKEFAPRLQLPLSLSYD
CCCCEEEEECCCCCCCCHHCCCCCCCCCEEEECCHHHCCCCCCCCCCCCEECCCEEECCC
HRVIDGAEGARFITFLNSALSDIRRLVL
CEEECCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TIEINVPDIGADEVEVTEILVKVGDRVEEEQSLITVEGDKASMEVPASQAGIVKEIKIA
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCEEEEEEC
EGDSVTTGSLIMIFEAEGAAEAPAAPAVEAAPVAAPAAASVAELKEVHVPDIGGDEVEVT
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEHE
EIMVAVGDTVEEEQSLLTVEGDKASMEVPAPFAGTVKEIKIASGDSVSTGSLVMIFEVAG
EEEEHHCCCHHCCCCEEEEECCCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEECC
SAPAAPEVEAAAPVAAAPAASAEKEVNVPDIGGDEVEVTEIMVAVGDTVEEEQSLITVEG
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEHEEEEEHHCCCCCCCCCEEEEEC
DKASMEVPAPFAGTVKEIKIAAGDKVSTGSLIMTFVVEGTAPAAPQAAAAPAAQAAPAAA
CCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCHHCCCCHHCCCCCC
PVATASAPASTGEFEANNDYAHASPVVRRLAREFGVNLAKVKGTGRKNRILKEDVQGFVK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHH
DALKRLESGAAASGKGGDGSALGLLPWPKVDFSKFGETEVQKLSKIKKISGANLHRNWVM
HHHHHHHCCCCCCCCCCCCCEEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCEEE
IPHVTQWDNADITELEAFRKEQNAIEAKNDTGMKITPLVFIMKAVAKALEAFPAFNSSLS
ECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHH
EDGESIILKKYVNVGIAVDTPNGLVVPVFKDVNKKGIYELSEELMAVSKKARAGKLTASD
CCCHHEEEEECCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
MQGGCFTISSLGGIGGTAFTPIVNAPEVGILGVSKSEIKPVWNGKEFAPRLQLPLSLSYD
CCCCEEEEECCCCCCCCHHCCCCCCCCCEEEECCHHHCCCCCCCCCCCCEECCCEEECCC
HRVIDGAEGARFITFLNSALSDIRRLVL
CEEECCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]