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The map label for this gene is lpd [H]

Identifier: 59712785

GI number: 59712785

Start: 2447230

End: 2448657

Strand: Reverse

Name: lpd [H]

Synonym: VF_2178

Alternate gene names: 59712785

Gene position: 2448657-2447230 (Counterclockwise)

Preceding gene: 59712786

Following gene: 59712783

Centisome position: 84.51

GC content: 41.88

Gene sequence:

>1428_bases
ATGAGCAAACAAGTTAAAGCCCAAGTAGTTGTACTTGGTTCAGGTCCTGCTGGTTACTCTGCTGCTTTCCGTTGCGCAGA
TTTAGGTCTAGAAACAGTATTAATCGAGCGTTACAGCACTCTTGGTGGTGTATGTCTAAACGTGGGTTGTATTCCATCGA
AAGCTCTTCTTCACGTATCTAAAGTAATCGAAGAAGCAAAAGCAATGGCTGAACACGGTGTTGTTTTTGGTGAACCACAA
ACTGATATTAACAAGATCCGTATCTGGAAAGATAAAGTTGTAACTCAACTGACCGGCGGTCTTGGTGGTATGGCTAAGAT
GCGTAACGTTACTGTAGTAAATGGCTACGGTAAATTTACAGGCCCTAACACAATCGAAGTTGAAGGCGAAGAAAACACAA
CAGTTACTTTTGATAACGCTATCATTGCAGCTGGTTCTCGTCCGATTAAACTTCCGTTCATTCCGCATGAAGACCCACGT
ATTTGGGATTCAACTGATGCGCTAGAATTAAAAGAAGTACCAGAAAAATTATTGATCATGGGCGGTGGTATCATCGGTCT
TGAAATGGGTACGGTTTACCACTCTTTAGGTTCTAAAGTTGAAGTGGTTGAGATGTTTGATCAAGTTATCCCAGCAGCTG
ATAAAGACATCGTTAAAGTATACACAAAACGCATTAAAGACAAATTCAAGCTAATGCTTGAGACAAAAGTAACAGCAGTT
GAAGCAAAAGAAGACGGTATCTATGTTTCAATGGAAGGCAAAAAAGCACCAGCTGAAGCTGAGCGTTACGATGCAGTTCT
TGTTGCTATCGGTCGTGTACCAAACGGTAAACTTATCGATGCTGAAAAAGCAGGTATCGAAGTTGATGAGCGTGGTTTCA
TCAATGTTGATAAGCAAATGCGTACTAACGTTGCTCACATTCATGCGATCGGTGATGTTGTTGGTCAACCAATGCTTGCT
CACAAAGGTGTGCATGAAGGTCACGTAGCGGCTGAAGTTATTTCTGGTAAGAAGCACTACTTCGATCCTAAAGTAATTCC
TTCAATTGCTTACACTGAGCCAGAAGTTGCTTGGGTTGGTAAAACTGAGAAAGAAGCAAAAGAAGAAGGTCTAAACTTTG
AAGTTGCAACTTTCCCATGGGCTGCATCAGGTCGTGCAATCGCATCTGATTGTGCTGATGGTATGACTAAGCTTATCTTT
GATAAAGAAACTCACCGTGTAATTGGTGGTGCTATCGTTGGTACTAACGGTGGTGAGCTACTTGGTGAAATCGGCCTAGC
AATCGAAATGGGTTGTGACGCAGAAGATATCGCTCTTACTATCCACGCTCACCCAACTCTACATGAGTCTGTGGGTCTAG
CTGCGGAAGTATTTGAAGGTTCAATCACTGACCTTCCAAACAAAAAAGCAGTTAAAAAGAAAAAATAA

Upstream 100 bases:

>100_bases
TGCTTTCACACTGTTAACATATATGTAAAATGAATGCGGTTTGAAAACAATAATAGAACATCTGTCAGCCTGTTAGGGAA
ATGACTATAACGAGGTCAAA

Downstream 100 bases:

>100_bases
TTCTGATTAACAACGCATTTGTTAAGATATAAAAAACCGCTGAGAAATCAGCGGTTTTTTATTATTATCATTTCATGAAT
CCTGAAATTATTTATAAATA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSKQVKAQVVVLGSGPAGYSAAFRCADLGLETVLIERYSTLGGVCLNVGCIPSKALLHVSKVIEEAKAMAEHGVVFGEPQ
TDINKIRIWKDKVVTQLTGGLGGMAKMRNVTVVNGYGKFTGPNTIEVEGEENTTVTFDNAIIAAGSRPIKLPFIPHEDPR
IWDSTDALELKEVPEKLLIMGGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVYTKRIKDKFKLMLETKVTAV
EAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGKLIDAEKAGIEVDERGFINVDKQMRTNVAHIHAIGDVVGQPMLA
HKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKEEGLNFEVATFPWAASGRAIASDCADGMTKLIF
DKETHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNKKAVKKKK

Sequences:

>Translated_475_residues
MSKQVKAQVVVLGSGPAGYSAAFRCADLGLETVLIERYSTLGGVCLNVGCIPSKALLHVSKVIEEAKAMAEHGVVFGEPQ
TDINKIRIWKDKVVTQLTGGLGGMAKMRNVTVVNGYGKFTGPNTIEVEGEENTTVTFDNAIIAAGSRPIKLPFIPHEDPR
IWDSTDALELKEVPEKLLIMGGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVYTKRIKDKFKLMLETKVTAV
EAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGKLIDAEKAGIEVDERGFINVDKQMRTNVAHIHAIGDVVGQPMLA
HKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKEEGLNFEVATFPWAASGRAIASDCADGMTKLIF
DKETHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNKKAVKKKK
>Mature_474_residues
SKQVKAQVVVLGSGPAGYSAAFRCADLGLETVLIERYSTLGGVCLNVGCIPSKALLHVSKVIEEAKAMAEHGVVFGEPQT
DINKIRIWKDKVVTQLTGGLGGMAKMRNVTVVNGYGKFTGPNTIEVEGEENTTVTFDNAIIAAGSRPIKLPFIPHEDPRI
WDSTDALELKEVPEKLLIMGGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVYTKRIKDKFKLMLETKVTAVE
AKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGKLIDAEKAGIEVDERGFINVDKQMRTNVAHIHAIGDVVGQPMLAH
KGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKEEGLNFEVATFPWAASGRAIASDCADGMTKLIFD
KETHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNKKAVKKKK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=44.273127753304, Blast_Score=344, Evalue=9e-95,
Organism=Homo sapiens, GI50301238, Length=449, Percent_Identity=28.0623608017817, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI148277071, Length=460, Percent_Identity=26.5217391304348, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI148277065, Length=459, Percent_Identity=26.5795206971678, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519430, Length=459, Percent_Identity=26.5795206971678, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519428, Length=459, Percent_Identity=26.5795206971678, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519426, Length=459, Percent_Identity=26.5795206971678, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=453, Percent_Identity=27.5938189845475, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI22035672, Length=428, Percent_Identity=26.8691588785047, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI291045268, Length=442, Percent_Identity=26.2443438914027, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=86.7088607594937, Blast_Score=846, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=28.6937901498929, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=29.8901098901099, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789915, Length=447, Percent_Identity=29.7539149888143, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=38.8646288209607, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=28.1512605042017, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=435, Percent_Identity=26.2068965517241, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=435, Percent_Identity=26.2068965517241, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=435, Percent_Identity=25.7471264367816, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.4473684210526, Blast_Score=311, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=26.7094017094017, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=452, Percent_Identity=27.4336283185841, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=40.4814004376368, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24640553, Length=469, Percent_Identity=27.5053304904051, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640549, Length=469, Percent_Identity=27.5053304904051, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640551, Length=469, Percent_Identity=27.5053304904051, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17737741, Length=471, Percent_Identity=25.0530785562633, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51037; Mature: 50906

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQVKAQVVVLGSGPAGYSAAFRCADLGLETVLIERYSTLGGVCLNVGCIPSKALLHVS
CCCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAMAEHGVVFGEPQTDINKIRIWKDKVVTQLTGGLGGMAKMRNVTVVNGYGKFT
HHHHHHHHHHHCCEEECCCCCCCCEEEEEHHHHHHHHHCCCCCHHHHCEEEEEECCCCCC
GPNTIEVEGEENTTVTFDNAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPEKLLIM
CCCEEEEECCCCCEEEECCEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHCCHHEEEE
GGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVYTKRIKDKFKLMLETKVTAV
CCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEE
EAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGKLIDAEKAGIEVDERGFINVDKQM
EECCCCEEEEECCCCCCCHHHHHCEEEEEEECCCCCCEEECHHCCCEECCCCCEEECHHH
RTNVAHIHAIGDVVGQPMLAHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVG
HCCHHHEEEHHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEEC
KTEKEAKEEGLNFEVATFPWAASGRAIASDCADGMTKLIFDKETHRVIGGAIVGTNGGEL
CCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHEEECEEEEECCCHHH
LGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNKKAVKKKK
HHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKQVKAQVVVLGSGPAGYSAAFRCADLGLETVLIERYSTLGGVCLNVGCIPSKALLHVS
CCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAMAEHGVVFGEPQTDINKIRIWKDKVVTQLTGGLGGMAKMRNVTVVNGYGKFT
HHHHHHHHHHHCCEEECCCCCCCCEEEEEHHHHHHHHHCCCCCHHHHCEEEEEECCCCCC
GPNTIEVEGEENTTVTFDNAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPEKLLIM
CCCEEEEECCCCCEEEECCEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHCCHHEEEE
GGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVYTKRIKDKFKLMLETKVTAV
CCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEE
EAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGKLIDAEKAGIEVDERGFINVDKQM
EECCCCEEEEECCCCCCCHHHHHCEEEEEEECCCCCCEEECHHCCCEECCCCCEEECHHH
RTNVAHIHAIGDVVGQPMLAHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVG
HCCHHHEEEHHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEEC
KTEKEAKEEGLNFEVATFPWAASGRAIASDCADGMTKLIFDKETHRVIGGAIVGTNGGEL
CCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHEEECEEEEECCCHHH
LGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNKKAVKKKK
HHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]