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The map label for this gene is nikA [C]

Identifier: 59712746

GI number: 59712746

Start: 2391189

End: 2392862

Strand: Direct

Name: nikA [C]

Synonym: VF_2139

Alternate gene names: 59712746

Gene position: 2391189-2392862 (Clockwise)

Preceding gene: 59712745

Following gene: 59712747

Centisome position: 82.52

GC content: 41.4

Gene sequence:

>1674_bases
ATGCTTGCCAATATTAAAAAAACTACATTAGCTGCTGCAGTACTTGCAGCAACAACGGGTTTTGCAGCTACAGATGCAGC
GGCACGAAGTGAGCTAACAGTAGTACCAGATTTCTACCCTACAATGATCCAAAACTTTAACCCATACCTTGCAACTAACC
TTCGTACAACAACAGATTTTGTTTACGAACCACTAGTTATCTTTAATGAAATGCATGGTAACAAACCAGTTATGCGTCTT
GCGGAAGATTTCCGTATGTCTGATGACCTTATGAGTGTAACTTTTGACATTCGTAAAGGTGTTAAATGGTCTGATGGTGA
GAAATTCACAGCTGACGACGTAATTTACTCTTATAACCTACTAAAAGATAAGCCAGAGCTTGATCAACGCGGTATCAATA
AGTGGGTTACAAACGTTGAACGTGTAAATGAATACCAAGTTAAATTCAGCCTATCTGAAGCTAACTCAAACGTTCCATAC
GAAATTTCACTAGTTCCAATCGTTGCTGAGCACATTTGGTCTAAAGTGAAAGATCCAACAACATTTACAAATGAAAACCC
AGTAGGTACAGGTCCATTTACTGAGATCGATACTTTTACACCTCAACTTTACATTCAGTGTCGTAACCCTAACTACTGGG
ATAACGATAACCTAGATGTGGACTGTTTACGTGTACCTCAAATCGCAAACAACGACCAATTACTAGGTAAGATTGTTAAC
TCTGAACTAGACTGGACTTCTTCTTTCGTTCCAGATATCGATCGTACATACGCTTCAGCAAGTCCTAACCACCAATACTG
GTACCCGCCATCAGGTACACAAGCATTCATGGTTAACTTCAAAAACCCTGATGCAGCTAAAAACGAAGCGTTAACAAACG
TTGAGTTCCGTCGTGCTATGGGTATGGCTCTTGACCGTCAAACAATCATTGATATCGCATTCTACGGCGGCGGTACTGTG
AATGATTTCGCATCTGGTCTTGGTTATGCATTTGAAGCATGGTCTGATGAAGATACTCATAACAAGTATAAGAAATTCAA
CACATACAACGTTGAAGGCGCTAAAAAGCAACTAGCTAAAGCTGGCTTTAAAGACGTAAACGGCGATGGATATGTTGATA
CTCCATCAGGTAAACCTTTCGAGCTTCTAATCCAATCACCAAACGGCTGGACTGACTTTAACAACACAGTTCAACTTGCT
GTAGAGCAATTAGATGAAGTTGGTATCAAAGCGAAAGCTCGTACTCCTGAGTTCGCGGTATACAACCAAGCAATGCTAGA
AGGTACTTATGATGTAGCTTACACAAACTACTTCCACGGTGCCGATCCACACCTATACTGGAACAGTGCTTACAACTCAG
CACTTCAAGAAGGTGATGGTATGCCTCGTTTCGCAATGCACTACTTCAAAGATGCGAAACTTGATGCTCTACTTGATAGC
TTCTACAAAACAGCTGATCGTGATGAGCAATTAGAAATCGCTCATGGCATTCAGAAAATCATCGCTGAAAACCAAGTAAC
ACTCCCTGTGATGTCTGGTGCTTACATGTACCAATACAACACAAAACGCTTCAGCGGTTGGTGGAACGAGAAGAATCCTA
AGGGCCGTCCAAATATCTGGGCTGGTATCCCTGAGCGTCTTCTACACGTTCTTGACCTAAAACCTGTTAAGTAA

Upstream 100 bases:

>100_bases
AATTTGGAAAAATGGATTTGAGGTAAGCCTTTAATGTGTGTTTATCAAATTGATAAAGACGTATATATCGTGAAACTAAA
GCAAAGAGTAAGGAACAGCT

Downstream 100 bases:

>100_bases
TATGTAACTGTCTTTGCGGTGCACCCTTTGTGCACCGCCTATTCCCCCCCTTCCCTACTAGCTAAAGGAATGGCGCTCGT
CGTCAGGGGATTTTATGTCC

Product: chitooligosaccharide-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MLANIKKTTLAAAVLAATTGFAATDAAARSELTVVPDFYPTMIQNFNPYLATNLRTTTDFVYEPLVIFNEMHGNKPVMRL
AEDFRMSDDLMSVTFDIRKGVKWSDGEKFTADDVIYSYNLLKDKPELDQRGINKWVTNVERVNEYQVKFSLSEANSNVPY
EISLVPIVAEHIWSKVKDPTTFTNENPVGTGPFTEIDTFTPQLYIQCRNPNYWDNDNLDVDCLRVPQIANNDQLLGKIVN
SELDWTSSFVPDIDRTYASASPNHQYWYPPSGTQAFMVNFKNPDAAKNEALTNVEFRRAMGMALDRQTIIDIAFYGGGTV
NDFASGLGYAFEAWSDEDTHNKYKKFNTYNVEGAKKQLAKAGFKDVNGDGYVDTPSGKPFELLIQSPNGWTDFNNTVQLA
VEQLDEVGIKAKARTPEFAVYNQAMLEGTYDVAYTNYFHGADPHLYWNSAYNSALQEGDGMPRFAMHYFKDAKLDALLDS
FYKTADRDEQLEIAHGIQKIIAENQVTLPVMSGAYMYQYNTKRFSGWWNEKNPKGRPNIWAGIPERLLHVLDLKPVK

Sequences:

>Translated_557_residues
MLANIKKTTLAAAVLAATTGFAATDAAARSELTVVPDFYPTMIQNFNPYLATNLRTTTDFVYEPLVIFNEMHGNKPVMRL
AEDFRMSDDLMSVTFDIRKGVKWSDGEKFTADDVIYSYNLLKDKPELDQRGINKWVTNVERVNEYQVKFSLSEANSNVPY
EISLVPIVAEHIWSKVKDPTTFTNENPVGTGPFTEIDTFTPQLYIQCRNPNYWDNDNLDVDCLRVPQIANNDQLLGKIVN
SELDWTSSFVPDIDRTYASASPNHQYWYPPSGTQAFMVNFKNPDAAKNEALTNVEFRRAMGMALDRQTIIDIAFYGGGTV
NDFASGLGYAFEAWSDEDTHNKYKKFNTYNVEGAKKQLAKAGFKDVNGDGYVDTPSGKPFELLIQSPNGWTDFNNTVQLA
VEQLDEVGIKAKARTPEFAVYNQAMLEGTYDVAYTNYFHGADPHLYWNSAYNSALQEGDGMPRFAMHYFKDAKLDALLDS
FYKTADRDEQLEIAHGIQKIIAENQVTLPVMSGAYMYQYNTKRFSGWWNEKNPKGRPNIWAGIPERLLHVLDLKPVK
>Mature_557_residues
MLANIKKTTLAAAVLAATTGFAATDAAARSELTVVPDFYPTMIQNFNPYLATNLRTTTDFVYEPLVIFNEMHGNKPVMRL
AEDFRMSDDLMSVTFDIRKGVKWSDGEKFTADDVIYSYNLLKDKPELDQRGINKWVTNVERVNEYQVKFSLSEANSNVPY
EISLVPIVAEHIWSKVKDPTTFTNENPVGTGPFTEIDTFTPQLYIQCRNPNYWDNDNLDVDCLRVPQIANNDQLLGKIVN
SELDWTSSFVPDIDRTYASASPNHQYWYPPSGTQAFMVNFKNPDAAKNEALTNVEFRRAMGMALDRQTIIDIAFYGGGTV
NDFASGLGYAFEAWSDEDTHNKYKKFNTYNVEGAKKQLAKAGFKDVNGDGYVDTPSGKPFELLIQSPNGWTDFNNTVQLA
VEQLDEVGIKAKARTPEFAVYNQAMLEGTYDVAYTNYFHGADPHLYWNSAYNSALQEGDGMPRFAMHYFKDAKLDALLDS
FYKTADRDEQLEIAHGIQKIIAENQVTLPVMSGAYMYQYNTKRFSGWWNEKNPKGRPNIWAGIPERLLHVLDLKPVK

Specific function: Possible binding-protein with either a transport or enzymatic activity [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=394, Percent_Identity=25.6345177664975, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787052, Length=467, Percent_Identity=22.0556745182013, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63076; Mature: 63076

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLANIKKTTLAAAVLAATTGFAATDAAARSELTVVPDFYPTMIQNFNPYLATNLRTTTDF
CCCCCHHHHHHHHHHHHHCCCHHCCHHCCCCEEECCCHHHHHHHCCCCCEECCCCCHHHH
VYEPLVIFNEMHGNKPVMRLAEDFRMSDDLMSVTFDIRKGVKWSDGEKFTADDVIYSYNL
HHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHEEHHHHCCCCCCCCCCEECCCEEEEEHH
LKDKPELDQRGINKWVTNVERVNEYQVKFSLSEANSNVPYEISLVPIVAEHIWSKVKDPT
HCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCCC
TFTNENPVGTGPFTEIDTFTPQLYIQCRNPNYWDNDNLDVDCLRVPQIANNDQLLGKIVN
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHC
SELDWTSSFVPDIDRTYASASPNHQYWYPPSGTQAFMVNFKNPDAAKNEALTNVEFRRAM
CCCCCCCCCCCCHHHHHCCCCCCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
GMALDRQTIIDIAFYGGGTVNDFASGLGYAFEAWSDEDTHNKYKKFNTYNVEGAKKQLAK
HHHCCCCEEEEEEEECCCCHHHHHHHCCCEEECCCCCCHHHHHHHCCCCCCCHHHHHHHH
AGFKDVNGDGYVDTPSGKPFELLIQSPNGWTDFNNTVQLAVEQLDEVGIKAKARTPEFAV
CCCCCCCCCCCEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCHHH
YNQAMLEGTYDVAYTNYFHGADPHLYWNSAYNSALQEGDGMPRFAMHYFKDAKLDALLDS
HHHHHHCCCEEEEEECCCCCCCCCEEECHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHH
FYKTADRDEQLEIAHGIQKIIAENQVTLPVMSGAYMYQYNTKRFSGWWNEKNPKGRPNIW
HHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCC
AGIPERLLHVLDLKPVK
CCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MLANIKKTTLAAAVLAATTGFAATDAAARSELTVVPDFYPTMIQNFNPYLATNLRTTTDF
CCCCCHHHHHHHHHHHHHCCCHHCCHHCCCCEEECCCHHHHHHHCCCCCEECCCCCHHHH
VYEPLVIFNEMHGNKPVMRLAEDFRMSDDLMSVTFDIRKGVKWSDGEKFTADDVIYSYNL
HHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHEEHHHHCCCCCCCCCCEECCCEEEEEHH
LKDKPELDQRGINKWVTNVERVNEYQVKFSLSEANSNVPYEISLVPIVAEHIWSKVKDPT
HCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCCC
TFTNENPVGTGPFTEIDTFTPQLYIQCRNPNYWDNDNLDVDCLRVPQIANNDQLLGKIVN
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHC
SELDWTSSFVPDIDRTYASASPNHQYWYPPSGTQAFMVNFKNPDAAKNEALTNVEFRRAM
CCCCCCCCCCCCHHHHHCCCCCCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
GMALDRQTIIDIAFYGGGTVNDFASGLGYAFEAWSDEDTHNKYKKFNTYNVEGAKKQLAK
HHHCCCCEEEEEEEECCCCHHHHHHHCCCEEECCCCCCHHHHHHHCCCCCCCHHHHHHHH
AGFKDVNGDGYVDTPSGKPFELLIQSPNGWTDFNNTVQLAVEQLDEVGIKAKARTPEFAV
CCCCCCCCCCCEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCHHH
YNQAMLEGTYDVAYTNYFHGADPHLYWNSAYNSALQEGDGMPRFAMHYFKDAKLDALLDS
HHHHHHCCCEEEEEECCCCCCCCCEEECHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHH
FYKTADRDEQLEIAHGIQKIIAENQVTLPVMSGAYMYQYNTKRFSGWWNEKNPKGRPNIW
HHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCC
AGIPERLLHVLDLKPVK
CCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]