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The map label for this gene is hemL [H]

Identifier: 59712742

GI number: 59712742

Start: 2383197

End: 2384492

Strand: Direct

Name: hemL [H]

Synonym: VF_2135

Alternate gene names: 59712742

Gene position: 2383197-2384492 (Clockwise)

Preceding gene: 59712738

Following gene: 59712743

Centisome position: 82.25

GC content: 42.05

Gene sequence:

>1296_bases
ATGACCAAATCAGCAGAACTTTTTGCAAAAGCGCAAGATAAAATCCCTGGCGGTGTAAACTCTCCTGTTCGTGCTTTTGC
TGGCGTTGGTGGCTCACCAATTTTCGTTGAGCGTGCCGATGGCCCTTTAATTTTTGATGCCGATGGCAAAGCTTATATTG
ATTATGTTGGTTCTTGGGGTCCAATGATCCTTGGTCATAACCATGCAGCAATTCGCGAAGCAGTTATCTCTGCAGCACAA
CGTGGTTTAAGTTTTGGTGCACCAACTGAAACTGAAATTACTATGGCTGAATTAGTTTCTGAACTCGTACCATCAATGGA
ACAAGTTCGCATGGTAAGTTCAGGTACAGAAGCAACAATGAGTGCGATTCGTTTGGCTCGTGGTTACACTGGACGTGATA
AAATCATGAAATTTGAAGGCTGTTACCATGGCCATGCTGATAGCTTACTTGTTAAAGCTGGTTCTGGTGCATTAACCCTA
GGTCAGCCAAGTTCTCCTGGTGTTCCTGCTGATTTTGCTAAATACACATTAACAGCAACTTTTAATGATTTAGATTCTGT
TCGTGAACTATTCGCTGTAAATAAAGGTGAAATCGCTTGTATTATTGTTGAGCCAGTAGCTGGTAACATGAACTGCATTC
CACCAGTAGAAGGGTTCCACGAAGGACTTCGTCAAATTTGTGATGAAGAAGGTGCTCTATTAATTTTTGATGAAGTAATG
ACAGGTTTCCGTGTGGCTGAAAACTGTGCTCAAGGTTACTACAACATCAAACCAGACCTAACAACATTAGGTAAAGTAAT
CGGCGGTGGTATGCCTGTTGGTGCGTTTGGCGGCCGTAAAGAAGTAATGCAATATATCGCCCCTACTGGCCCTGTTTACC
AAGCGGGTACACTTTCTGGTAACCCTGTTGCGATGGCTGCTGGTTTTGCCTGTTTAAAAGTATTAACTGAAGAAGGCAAC
GAAAAACGCCTTGCAGATACAACAAGACATCTTGCTAATGGTTTTAAAGAGTTAGCAAATAAACACGGTATTCCAATGGT
TGTAAACCAAGTGGGCGGAATGTTTGGTTTCTTCTTTACTGATCAAGAAACAGTAACCAGCTACGCTGATGTTGCTAAGT
GTGATATTGAAAGATTTAAACGCTTCTTCCACCTAATGCTTAAGAAAGGGGTATACCTAGCACCTTCTGCATTTGAAGCA
AGCTTCACTTCTTTAGCTCATGGTCCAAAAGAAATCGAAGCGACATTAGAAGCGGCAGATCAATGCTTCGCAATCATTGC
TTCAGAAGCAAAATAA

Upstream 100 bases:

>100_bases
CTATTTTACTATTAGATGGGGATTAATACGATCAACACAAGCGTTTTTAGGTACAATAGTGCCTAACTGGTCCAAATTAA
TTACTATGAGGATATAAGTA

Downstream 100 bases:

>100_bases
AACCTGATTAAAACAGATAAAGGCTTTCATTATTGAAAGCCTTTTTTCATATAACAAACCACTATAAAAAATCATAATGG
CCAATAAACAATTATTGCCA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTKSAELFAKAQDKIPGGVNSPVRAFAGVGGSPIFVERADGPLIFDADGKAYIDYVGSWGPMILGHNHAAIREAVISAAQ
RGLSFGAPTETEITMAELVSELVPSMEQVRMVSSGTEATMSAIRLARGYTGRDKIMKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKYTLTATFNDLDSVRELFAVNKGEIACIIVEPVAGNMNCIPPVEGFHEGLRQICDEEGALLIFDEVM
TGFRVAENCAQGYYNIKPDLTTLGKVIGGGMPVGAFGGRKEVMQYIAPTGPVYQAGTLSGNPVAMAAGFACLKVLTEEGN
EKRLADTTRHLANGFKELANKHGIPMVVNQVGGMFGFFFTDQETVTSYADVAKCDIERFKRFFHLMLKKGVYLAPSAFEA
SFTSLAHGPKEIEATLEAADQCFAIIASEAK

Sequences:

>Translated_431_residues
MTKSAELFAKAQDKIPGGVNSPVRAFAGVGGSPIFVERADGPLIFDADGKAYIDYVGSWGPMILGHNHAAIREAVISAAQ
RGLSFGAPTETEITMAELVSELVPSMEQVRMVSSGTEATMSAIRLARGYTGRDKIMKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKYTLTATFNDLDSVRELFAVNKGEIACIIVEPVAGNMNCIPPVEGFHEGLRQICDEEGALLIFDEVM
TGFRVAENCAQGYYNIKPDLTTLGKVIGGGMPVGAFGGRKEVMQYIAPTGPVYQAGTLSGNPVAMAAGFACLKVLTEEGN
EKRLADTTRHLANGFKELANKHGIPMVVNQVGGMFGFFFTDQETVTSYADVAKCDIERFKRFFHLMLKKGVYLAPSAFEA
SFTSLAHGPKEIEATLEAADQCFAIIASEAK
>Mature_430_residues
TKSAELFAKAQDKIPGGVNSPVRAFAGVGGSPIFVERADGPLIFDADGKAYIDYVGSWGPMILGHNHAAIREAVISAAQR
GLSFGAPTETEITMAELVSELVPSMEQVRMVSSGTEATMSAIRLARGYTGRDKIMKFEGCYHGHADSLLVKAGSGALTLG
QPSSPGVPADFAKYTLTATFNDLDSVRELFAVNKGEIACIIVEPVAGNMNCIPPVEGFHEGLRQICDEEGALLIFDEVMT
GFRVAENCAQGYYNIKPDLTTLGKVIGGGMPVGAFGGRKEVMQYIAPTGPVYQAGTLSGNPVAMAAGFACLKVLTEEGNE
KRLADTTRHLANGFKELANKHGIPMVVNQVGGMFGFFFTDQETVTSYADVAKCDIERFKRFFHLMLKKGVYLAPSAFEAS
FTSLAHGPKEIEATLEAADQCFAIIASEAK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=297, Percent_Identity=28.2828282828283, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI37574042, Length=347, Percent_Identity=26.5129682997118, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI226442705, Length=347, Percent_Identity=26.2247838616715, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI284507298, Length=211, Percent_Identity=30.3317535545024, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI226442709, Length=285, Percent_Identity=27.3684210526316, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=71.4622641509434, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=443, Percent_Identity=29.1196388261851, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787560, Length=377, Percent_Identity=29.973474801061, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788044, Length=333, Percent_Identity=30.03003003003, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI145693181, Length=368, Percent_Identity=29.0760869565217, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789759, Length=327, Percent_Identity=27.5229357798165, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786991, Length=390, Percent_Identity=27.9487179487179, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=300, Percent_Identity=26.3333333333333, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=390, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=32.8671328671329, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=340, Percent_Identity=29.1176470588235, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=349, Percent_Identity=24.3553008595989, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357415, Length=309, Percent_Identity=26.537216828479, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI161085790, Length=440, Percent_Identity=23.4090909090909, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28574759, Length=382, Percent_Identity=23.8219895287958, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45935; Mature: 45804

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSAELFAKAQDKIPGGVNSPVRAFAGVGGSPIFVERADGPLIFDADGKAYIDYVGSWG
CCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEECCCCEEECCCCCEEEEECCCCC
PMILGHNHAAIREAVISAAQRGLSFGAPTETEITMAELVSELVPSMEQVRMVSSGTEATM
CEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGYTGRDKIMKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKYTLTAT
HHHHHHCCCCCHHHHEEECCCCCCCCCCEEEECCCCEEECCCCCCCCCCCHHHHEEEEEE
FNDLDSVRELFAVNKGEIACIIVEPVAGNMNCIPPVEGFHEGLRQICDEEGALLIFDEVM
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHH
TGFRVAENCAQGYYNIKPDLTTLGKVIGGGMPVGAFGGRKEVMQYIAPTGPVYQAGTLSG
HHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAMAAGFACLKVLTEEGNEKRLADTTRHLANGFKELANKHGIPMVVNQVGGMFGFFFT
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEC
DQETVTSYADVAKCDIERFKRFFHLMLKKGVYLAPSAFEASFTSLAHGPKEIEATLEAAD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHH
QCFAIIASEAK
HHHHHHHCCCC
>Mature Secondary Structure 
TKSAELFAKAQDKIPGGVNSPVRAFAGVGGSPIFVERADGPLIFDADGKAYIDYVGSWG
CCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEECCCCEEECCCCCEEEEECCCCC
PMILGHNHAAIREAVISAAQRGLSFGAPTETEITMAELVSELVPSMEQVRMVSSGTEATM
CEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGYTGRDKIMKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKYTLTAT
HHHHHHCCCCCHHHHEEECCCCCCCCCCEEEECCCCEEECCCCCCCCCCCHHHHEEEEEE
FNDLDSVRELFAVNKGEIACIIVEPVAGNMNCIPPVEGFHEGLRQICDEEGALLIFDEVM
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHH
TGFRVAENCAQGYYNIKPDLTTLGKVIGGGMPVGAFGGRKEVMQYIAPTGPVYQAGTLSG
HHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAMAAGFACLKVLTEEGNEKRLADTTRHLANGFKELANKHGIPMVVNQVGGMFGFFFT
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEC
DQETVTSYADVAKCDIERFKRFFHLMLKKGVYLAPSAFEASFTSLAHGPKEIEATLEAAD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHH
QCFAIIASEAK
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA