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The map label for this gene is lepA [H]

Identifier: 59712696

GI number: 59712696

Start: 2326082

End: 2327875

Strand: Reverse

Name: lepA [H]

Synonym: VF_2089

Alternate gene names: 59712696

Gene position: 2327875-2326082 (Counterclockwise)

Preceding gene: 59712697

Following gene: 59712695

Centisome position: 80.34

GC content: 41.14

Gene sequence:

>1794_bases
ATGAAGCACATTCGTAATTTTTCTATTATTGCCCATATCGATCACGGTAAATCGACACTATCTGACCGCCTAATCCAAGT
TTGTGGTGGCCTGTCAGACCGTGAAATGGCAGCGCAAGTTCTTGATTCAATGGATCTAGAACGTGAACGTGGTATCACCA
TCAAAGCCCAGAGTGTGACGCTCGACTACACGGCTAAAGATGGTGAGACTTATCAACTAAACTTTATCGACACACCTGGA
CACGTTGACTTCTCTTACGAAGTATCGCGTTCACTTGCTGCCTGTGAAGGTGCGCTATTGGTTGTCGATGCGGGTCAAGG
TGTAGAAGCACAGACTCTAGCAAACTGTTACACAGCTATCGAAATGGACCTAGAAGTAGTTCCAATCTTAAACAAGATTG
ACTTACCTGCAGCAGATCCAGATCGTGTAGCTGAAGAAATCGAAGAGATCGTTGGTATTGATGCAACAGACGCAACGCGC
TGTTCAGCAAAAACGGGTTTAGGTGTTGATGAAGTTCTTGAAACTATCGTTAAATCTATTCCTGCACCAGAAGGCGACCC
TGAAGCACCAACACAAGCACTTATTATCGACTCATGGTTCGATAACTACTTAGGTGTTGTTTCTCTAGTTCGAATCAAAA
ATGGTTCACTGAAGAAAAATGACAAGATCAAAGTAATGAGTACTGGCCAAGTTTGGGGTATCGACCGTATTGGTATCTTC
ACTCCAAAACAAATCGATACTGATGTATTGAATACTGGCGAAGTAGGTTGGGTTGTTTGTGGTATCAAAGATATTCTTGG
TGCGCCAGTAGGTGATACATTGACACACGCGAAAGGCGGGTGTGAAGAGCGTTTACCTGGTTTCCAAAAAGTAAAACCAC
AAGTATATGCAGGTTTATTCCCTGTATCTTCTGATGATTACGAAAACTTCCGTGATGCACTTGGTAAATTAAGCCTAAAC
GATGCTTCACTTTTCTATGAACCAGAAAGTTCAGCTGCACTTGGTTTTGGTTTCCGTTGTGGCTTCCTTGGCATGCTTCA
CATGGAAATTATCCAAGAGCGTCTAGAGCGTGAATACGATCTTGACTTAATCACAACAGCACCAACAGTAGTTTACGAAG
TAGCAAAAACTGATGGTGAAATTCTGTATGTAGATAGTCCTGCGAAATTACCAGCAGTTAATGATATTGATGAGATCCGT
GAACCAATTGCTCGTTGTAATATCTTAGTACCATCAGACTACTTAGGTAATGTAATCACACTTTGTGTAGAGAAGCGTGG
CGTACAAGTGGACATGCAGTACCACGGTAACCAAGTAGCGGTAACTTATGATGTTCCAATGGCTGAAGTAGTTTTAGACT
TCTTTGACCGTTTGAAATCAACATCTCGTGGTTATGCATCACTTGATTACAACTTCCAACGTTATGAAGCATCAAGCATG
GTTCGTGTTGATGTTCTATTAAACGGCGACAAAGTGGATGCTCTAGCTATCATTACTCACAAAGACAACTCACAATCTCG
TGGTCGTCTATTAGTTGAGAAGATGAAAGAATTTATCCCTCGTCAAATGTTTGATATTGCAATTCAAGCAGCGATTGGTA
ACCACATTATTGCTCGTTCAACAGTAAAACAATTACGTAAAAACGTAATCGCTAAATGTTATGGTGGTGACGTAAGTCGT
AAGAAGAAACTGTTGAAGAAGCAGAAAGAAGGTAAGAAACGTATGAAGCAAATTGGTAATGTTGAATTACCACAAGAAGC
TTTCCTTGCGATTCTTCATGTAGGTAAAGATTAA

Upstream 100 bases:

>100_bases
CTGCACGATAAGATAGCGACTTGCTCAGAAATTGGGTAGAATCCCACAACTTGATCTCACGATCCAATCCTATTAACTAA
TTGAGTTTGTCACATCATTC

Downstream 100 bases:

>100_bases
TTTTCTGATTTCTGAATCCTCAGAGTTCTGTAAATTTAAGCTTTATTACAGCTCAAAGAAAGCGATGATCAATGGTCTTC
GCTTTCTTTGTTATGCAACA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMAAQVLDSMDLERERGITIKAQSVTLDYTAKDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMDLEVVPILNKIDLPAADPDRVAEEIEEIVGIDATDATR
CSAKTGLGVDEVLETIVKSIPAPEGDPEAPTQALIIDSWFDNYLGVVSLVRIKNGSLKKNDKIKVMSTGQVWGIDRIGIF
TPKQIDTDVLNTGEVGWVVCGIKDILGAPVGDTLTHAKGGCEERLPGFQKVKPQVYAGLFPVSSDDYENFRDALGKLSLN
DASLFYEPESSAALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVYEVAKTDGEILYVDSPAKLPAVNDIDEIR
EPIARCNILVPSDYLGNVITLCVEKRGVQVDMQYHGNQVAVTYDVPMAEVVLDFFDRLKSTSRGYASLDYNFQRYEASSM
VRVDVLLNGDKVDALAIITHKDNSQSRGRLLVEKMKEFIPRQMFDIAIQAAIGNHIIARSTVKQLRKNVIAKCYGGDVSR
KKKLLKKQKEGKKRMKQIGNVELPQEAFLAILHVGKD

Sequences:

>Translated_597_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMAAQVLDSMDLERERGITIKAQSVTLDYTAKDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMDLEVVPILNKIDLPAADPDRVAEEIEEIVGIDATDATR
CSAKTGLGVDEVLETIVKSIPAPEGDPEAPTQALIIDSWFDNYLGVVSLVRIKNGSLKKNDKIKVMSTGQVWGIDRIGIF
TPKQIDTDVLNTGEVGWVVCGIKDILGAPVGDTLTHAKGGCEERLPGFQKVKPQVYAGLFPVSSDDYENFRDALGKLSLN
DASLFYEPESSAALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVYEVAKTDGEILYVDSPAKLPAVNDIDEIR
EPIARCNILVPSDYLGNVITLCVEKRGVQVDMQYHGNQVAVTYDVPMAEVVLDFFDRLKSTSRGYASLDYNFQRYEASSM
VRVDVLLNGDKVDALAIITHKDNSQSRGRLLVEKMKEFIPRQMFDIAIQAAIGNHIIARSTVKQLRKNVIAKCYGGDVSR
KKKLLKKQKEGKKRMKQIGNVELPQEAFLAILHVGKD
>Mature_597_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMAAQVLDSMDLERERGITIKAQSVTLDYTAKDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMDLEVVPILNKIDLPAADPDRVAEEIEEIVGIDATDATR
CSAKTGLGVDEVLETIVKSIPAPEGDPEAPTQALIIDSWFDNYLGVVSLVRIKNGSLKKNDKIKVMSTGQVWGIDRIGIF
TPKQIDTDVLNTGEVGWVVCGIKDILGAPVGDTLTHAKGGCEERLPGFQKVKPQVYAGLFPVSSDDYENFRDALGKLSLN
DASLFYEPESSAALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVYEVAKTDGEILYVDSPAKLPAVNDIDEIR
EPIARCNILVPSDYLGNVITLCVEKRGVQVDMQYHGNQVAVTYDVPMAEVVLDFFDRLKSTSRGYASLDYNFQRYEASSM
VRVDVLLNGDKVDALAIITHKDNSQSRGRLLVEKMKEFIPRQMFDIAIQAAIGNHIIARSTVKQLRKNVIAKCYGGDVSR
KKKLLKKQKEGKKRMKQIGNVELPQEAFLAILHVGKD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=46.5890183028286, Blast_Score=587, Evalue=1e-167,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=45.5223880597015, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=43.6241610738255, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI19923640, Length=149, Percent_Identity=43.6241610738255, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI25306287, Length=149, Percent_Identity=43.6241610738255, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=42.3611111111111, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI18390331, Length=158, Percent_Identity=37.3417721518987, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=44.1441441441441, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=44.1441441441441, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=44.1441441441441, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272894, Length=168, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI217272892, Length=168, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI53729339, Length=239, Percent_Identity=29.7071129707113, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI53729337, Length=239, Percent_Identity=29.7071129707113, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=84.7571189279732, Blast_Score=1022, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=514, Percent_Identity=28.0155642023346, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=35.4838709677419, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.0512820512821, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1789559, Length=224, Percent_Identity=30.8035714285714, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=40.1639344262295, Blast_Score=481, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=35.4838709677419, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=35.6164383561644, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=38.5185185185185, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=170, Percent_Identity=35.2941176470588, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=38.5185185185185, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.8620689655172, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=240, Percent_Identity=28.75, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=46.1794019933555, Blast_Score=533, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6323098, Length=158, Percent_Identity=38.6075949367089, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=42.3611111111111, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=42.3611111111111, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=38.2608695652174, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=39.3103448275862, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=338, Percent_Identity=27.2189349112426, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325337, Length=171, Percent_Identity=30.9941520467836, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=171, Percent_Identity=30.9941520467836, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=43.261231281198, Blast_Score=529, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=37.2670807453416, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=44.6043165467626, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585709, Length=162, Percent_Identity=35.8024691358025, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585711, Length=162, Percent_Identity=35.8024691358025, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585713, Length=162, Percent_Identity=35.8024691358025, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=37.5838926174497, Blast_Score=92, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357743, Length=165, Percent_Identity=32.7272727272727, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281363316, Length=324, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864358, Length=324, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28572034, Length=224, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 65865; Mature: 65865

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMAAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYTAKDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAI
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPILNKIDLPAADPDRVAEEIEEIVGIDATDATRCSAKTGLGVDEVLETIVKSI
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PAPEGDPEAPTQALIIDSWFDNYLGVVSLVRIKNGSLKKNDKIKVMSTGQVWGIDRIGIF
CCCCCCCCCCCEEEEEEHHHHHHHHHHHEEEECCCCCCCCCCEEEEECCCEECCCEECCC
TPKQIDTDVLNTGEVGWVVCGIKDILGAPVGDTLTHAKGGCEERLPGFQKVKPQVYAGLF
CCCCCCCHHCCCCCCCEEEEEHHHHHCCCCCCHHHHCCCCHHHHCCCHHHCCCHHHEEEE
PVSSDDYENFRDALGKLSLNDASLFYEPESSAALGFGFRCGFLGMLHMEIIQERLEREYD
CCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVAKTDGEILYVDSPAKLPAVNDIDEIREPIARCNILVPSDYLGNVIT
CEEEECCCHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCEEECCCHHHHHHHH
LCVEKRGVQVDMQYHGNQVAVTYDVPMAEVVLDFFDRLKSTSRGYASLDYNFQRYEASSM
HHHHCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCE
VRVDVLLNGDKVDALAIITHKDNSQSRGRLLVEKMKEFIPRQMFDIAIQAAIGNHIIARS
EEEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TVKQLRKNVIAKCYGGDVSRKKKLLKKQKEGKKRMKQIGNVELPQEAFLAILHVGKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLSDREMAAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYTAKDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAI
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPILNKIDLPAADPDRVAEEIEEIVGIDATDATRCSAKTGLGVDEVLETIVKSI
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PAPEGDPEAPTQALIIDSWFDNYLGVVSLVRIKNGSLKKNDKIKVMSTGQVWGIDRIGIF
CCCCCCCCCCCEEEEEEHHHHHHHHHHHEEEECCCCCCCCCCEEEEECCCEECCCEECCC
TPKQIDTDVLNTGEVGWVVCGIKDILGAPVGDTLTHAKGGCEERLPGFQKVKPQVYAGLF
CCCCCCCHHCCCCCCCEEEEEHHHHHCCCCCCHHHHCCCCHHHHCCCHHHCCCHHHEEEE
PVSSDDYENFRDALGKLSLNDASLFYEPESSAALGFGFRCGFLGMLHMEIIQERLEREYD
CCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVAKTDGEILYVDSPAKLPAVNDIDEIREPIARCNILVPSDYLGNVIT
CEEEECCCHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCEEECCCHHHHHHHH
LCVEKRGVQVDMQYHGNQVAVTYDVPMAEVVLDFFDRLKSTSRGYASLDYNFQRYEASSM
HHHHCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCE
VRVDVLLNGDKVDALAIITHKDNSQSRGRLLVEKMKEFIPRQMFDIAIQAAIGNHIIARS
EEEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
TVKQLRKNVIAKCYGGDVSRKKKLLKKQKEGKKRMKQIGNVELPQEAFLAILHVGKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA