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The map label for this gene is rumA [H]

Identifier: 59712688

GI number: 59712688

Start: 2317117

End: 2318466

Strand: Reverse

Name: rumA [H]

Synonym: VF_2081

Alternate gene names: 59712688

Gene position: 2318466-2317117 (Counterclockwise)

Preceding gene: 59712690

Following gene: 59712687

Centisome position: 80.02

GC content: 38.07

Gene sequence:

>1350_bases
ATGGAGCTAATTTTTGTAAACCAGGCAGTAATAATGGCGCAATTCTTCAAACCGAAAAAGAAAGCTTCTGTAAACACAAA
ACATCAATCAGTTGATGTGGTTCGATTAGATCACAACGGAGCTGGAATTGCTTTTGTTGATAAGAAACCCGTGTTTATCG
AGGGAGCATTGCCTGGTGAAAAAGCGATCATTCAGTTTATTGAGCAAAAGAAACAATTTTCTCGTGCAAAACTGATTAAG
CTAGCGCAGAAAAGTGAAAAACGCCAAACACCGATTTGCCAGCATTATCATGAATGTGGTGGGTGTAACTTACAGCATCT
ACAACATGAAGAACAAATTGTAGCGAAAAATGAAAAGCTGCAAGAACTGATGAAAAAGCAAGGTGTCTCACAAGGTGAGA
TGGTTCAGCCTATTATGGGTGAGGAACTGAGTTACCGCCGTCGAGCTCGCATTAGCTTGATGCTCAATAAACAAACCAAT
CAGCTTGACTTTGGTTTTCGTAAAAAACAGAGCAAAGCAATTGTAAATGTTCGCCATTGCCCTGTTCTTGTTCAAGAGTT
AGACCAACATTTAGAGTCATTATTTACTTTATTAAATCAGTTAAAAGGTAAAAAGCACCTAGGTCATGTTGAGCTTGTAC
AAGCAGATAATGGCAGTGTTTTACTTATTCGTCATGTTGCAGATTTTAATGAGAAGGATCAGCAAGCGTTAGTTAATTAT
TGTGAAGAACGCAATTTAATCCTATACCTAATGCCTGAAGCGGATGTGTTAAATCATGTTCGTGGAGAAGAACCATTTTA
TCTTATTGATGGCACCAAAATTTATTTTACGCCGAAAGATTTCATTCAAGTAAATCGTAATGTGAATGAGCAAATGGTTG
AGCAAGCTTTATCTTGGTTAGATTTAAATGAAAATGACAGCGTATTGGATCTGTTTTGTGGGTTAGGAAACTTTAGTCTA
CCATTAGCTAAAAAAGTAAAAACAGTGGTGGGAATTGAAGGTGTTGATGAAATGGTTCAGCGAGCAAAACTGAACGCAGA
AAGAAATCAACTTTCTAATGTGTCGTTTTATCAAGCTAACTTAGAAGAAGAGGTGAGTGAACAAGTGTGGGCTAGCACAA
AGTTTACAAAAATTTTACTGGATCCTGCTAGAGCAGGGGCTGCAGGTGTGATGGAAACTGTGGCAAAATTAAAACCTCAA
ACCGTGGTTTATGTTTCGTGTAACCCAGCAACACTTGCACGCGATAGCCAATTACTTATTCAACACGGTTTCAAGCTAAC
TAGGCTAGGTATGCTTGATATGTTTCCTCATACAGGGCATTTAGAATCAATGGCATTGTTTGAACGTTAA

Upstream 100 bases:

>100_bases
GCGTAGCTTAATCAACTTAGAGGCTAAGCTCAATTATTCTCTGTCGAAAGCGCAAGATAGAACGTAAATCAAGACTGCAT
CTTCATATGGTTTCGGTATA

Downstream 100 bases:

>100_bases
CTCACTCTTGAGTTCGTTAGGCGGGATCAAAAGAATTACAGATTAGAGAATTAATAGGAAACGGACATGGTCGCAGTTCG
CGGAGCACATCTAAAGGAAA

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MELIFVNQAVIMAQFFKPKKKASVNTKHQSVDVVRLDHNGAGIAFVDKKPVFIEGALPGEKAIIQFIEQKKQFSRAKLIK
LAQKSEKRQTPICQHYHECGGCNLQHLQHEEQIVAKNEKLQELMKKQGVSQGEMVQPIMGEELSYRRRARISLMLNKQTN
QLDFGFRKKQSKAIVNVRHCPVLVQELDQHLESLFTLLNQLKGKKHLGHVELVQADNGSVLLIRHVADFNEKDQQALVNY
CEERNLILYLMPEADVLNHVRGEEPFYLIDGTKIYFTPKDFIQVNRNVNEQMVEQALSWLDLNENDSVLDLFCGLGNFSL
PLAKKVKTVVGIEGVDEMVQRAKLNAERNQLSNVSFYQANLEEEVSEQVWASTKFTKILLDPARAGAAGVMETVAKLKPQ
TVVYVSCNPATLARDSQLLIQHGFKLTRLGMLDMFPHTGHLESMALFER

Sequences:

>Translated_449_residues
MELIFVNQAVIMAQFFKPKKKASVNTKHQSVDVVRLDHNGAGIAFVDKKPVFIEGALPGEKAIIQFIEQKKQFSRAKLIK
LAQKSEKRQTPICQHYHECGGCNLQHLQHEEQIVAKNEKLQELMKKQGVSQGEMVQPIMGEELSYRRRARISLMLNKQTN
QLDFGFRKKQSKAIVNVRHCPVLVQELDQHLESLFTLLNQLKGKKHLGHVELVQADNGSVLLIRHVADFNEKDQQALVNY
CEERNLILYLMPEADVLNHVRGEEPFYLIDGTKIYFTPKDFIQVNRNVNEQMVEQALSWLDLNENDSVLDLFCGLGNFSL
PLAKKVKTVVGIEGVDEMVQRAKLNAERNQLSNVSFYQANLEEEVSEQVWASTKFTKILLDPARAGAAGVMETVAKLKPQ
TVVYVSCNPATLARDSQLLIQHGFKLTRLGMLDMFPHTGHLESMALFER
>Mature_449_residues
MELIFVNQAVIMAQFFKPKKKASVNTKHQSVDVVRLDHNGAGIAFVDKKPVFIEGALPGEKAIIQFIEQKKQFSRAKLIK
LAQKSEKRQTPICQHYHECGGCNLQHLQHEEQIVAKNEKLQELMKKQGVSQGEMVQPIMGEELSYRRRARISLMLNKQTN
QLDFGFRKKQSKAIVNVRHCPVLVQELDQHLESLFTLLNQLKGKKHLGHVELVQADNGSVLLIRHVADFNEKDQQALVNY
CEERNLILYLMPEADVLNHVRGEEPFYLIDGTKIYFTPKDFIQVNRNVNEQMVEQALSWLDLNENDSVLDLFCGLGNFSL
PLAKKVKTVVGIEGVDEMVQRAKLNAERNQLSNVSFYQANLEEEVSEQVWASTKFTKILLDPARAGAAGVMETVAKLKPQ
TVVYVSCNPATLARDSQLLIQHGFKLTRLGMLDMFPHTGHLESMALFER

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=206, Percent_Identity=34.9514563106796, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI51173878, Length=206, Percent_Identity=34.9514563106796, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI269784680, Length=195, Percent_Identity=27.6923076923077, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI269784676, Length=195, Percent_Identity=27.6923076923077, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI20149688, Length=195, Percent_Identity=27.6923076923077, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI269784678, Length=195, Percent_Identity=27.6923076923077, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789148, Length=438, Percent_Identity=47.4885844748858, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1787083, Length=387, Percent_Identity=23.7726098191214, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790403, Length=180, Percent_Identity=32.2222222222222, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24666579, Length=201, Percent_Identity=28.3582089552239, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 51055; Mature: 51055

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIFVNQAVIMAQFFKPKKKASVNTKHQSVDVVRLDHNGAGIAFVDKKPVFIEGALPGE
CEEEEECHHHHHHHHHCCHHCCCCCCCCCCEEEEEEECCCCEEEEECCCCEEEECCCCCH
KAIIQFIEQKKQFSRAKLIKLAQKSEKRQTPICQHYHECGGCNLQHLQHEEQIVAKNEKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QELMKKQGVSQGEMVQPIMGEELSYRRRARISLMLNKQTNQLDFGFRKKQSKAIVNVRHC
HHHHHHCCCCCCCCCCCHHCCHHHHHHHCEEEEEEECCCCCCCCCHHHCCCCCEEEEHHH
PVLVQELDQHLESLFTLLNQLKGKKHLGHVELVQADNGSVLLIRHVADFNEKDQQALVNY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHH
CEERNLILYLMPEADVLNHVRGEEPFYLIDGTKIYFTPKDFIQVNRNVNEQMVEQALSWL
HHCCCEEEEECCCHHHHHHHCCCCCEEEEECCEEEECCHHHHHHCCCCCHHHHHHHHHHC
DLNENDSVLDLFCGLGNFSLPLAKKVKTVVGIEGVDEMVQRAKLNAERNQLSNVSFYQAN
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCCCEEEHHC
LEEEVSEQVWASTKFTKILLDPARAGAAGVMETVAKLKPQTVVYVSCNPATLARDSQLLI
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHH
QHGFKLTRLGMLDMFPHTGHLESMALFER
HCCCEEEEECHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MELIFVNQAVIMAQFFKPKKKASVNTKHQSVDVVRLDHNGAGIAFVDKKPVFIEGALPGE
CEEEEECHHHHHHHHHCCHHCCCCCCCCCCEEEEEEECCCCEEEEECCCCEEEECCCCCH
KAIIQFIEQKKQFSRAKLIKLAQKSEKRQTPICQHYHECGGCNLQHLQHEEQIVAKNEKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QELMKKQGVSQGEMVQPIMGEELSYRRRARISLMLNKQTNQLDFGFRKKQSKAIVNVRHC
HHHHHHCCCCCCCCCCCHHCCHHHHHHHCEEEEEEECCCCCCCCCHHHCCCCCEEEEHHH
PVLVQELDQHLESLFTLLNQLKGKKHLGHVELVQADNGSVLLIRHVADFNEKDQQALVNY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHH
CEERNLILYLMPEADVLNHVRGEEPFYLIDGTKIYFTPKDFIQVNRNVNEQMVEQALSWL
HHCCCEEEEECCCHHHHHHHCCCCCEEEEECCEEEECCHHHHHHCCCCCHHHHHHHHHHC
DLNENDSVLDLFCGLGNFSLPLAKKVKTVVGIEGVDEMVQRAKLNAERNQLSNVSFYQAN
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCCCEEEHHC
LEEEVSEQVWASTKFTKILLDPARAGAAGVMETVAKLKPQTVVYVSCNPATLARDSQLLI
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHH
QHGFKLTRLGMLDMFPHTGHLESMALFER
HCCCEEEEECHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA