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The map label for this gene is relA [H]

Identifier: 59712687

GI number: 59712687

Start: 2314813

End: 2317050

Strand: Reverse

Name: relA [H]

Synonym: VF_2080

Alternate gene names: 59712687

Gene position: 2317050-2314813 (Counterclockwise)

Preceding gene: 59712688

Following gene: 59712686

Centisome position: 79.97

GC content: 41.15

Gene sequence:

>2238_bases
ATGGTCGCAGTTCGCGGAGCACATCTAAAGGAAAATGAAACGTTCGAGTTAGCTGAATGGATAGACAGCCTCGAACAAGA
TAAACCCACTTCAAAAAAACTGACAGAGGTTTACCAGCATTGTCAGGAGTTAGTGGCGTCCCATGAAAATGGTCAGCTAT
TGCTATGGCGTGGCCGAGAGATGATTGAAATTTTAATCACTCTAAGTATGGATAAAGCCACACTGGTTGCTGCACAACTT
TTCCCATTGGTGTCGAGTGGTCTTCTTGATAAAGAAACCCTCACAGAGCATTACAGTAAAGAAATTGAAAAGTTAATTGA
AGGCGTCGAAGAGATGGACTCAATTGGACAACTTAATGCTACTGTAGGGCCTGAGTCAGCATCAAACCAAGTCGATAATA
TTCGTCGTATGCTACTGGCTATGGTGGATGATTTTCGTTGTGTGATCATTAAATTAGCGGAGCGTATTTGTAACTTACGT
GAAGTGAAAAATGAGCCAGATGAAGTGCGTCGTGCTGCTGCGAAAGAGTGTAATAATATTTATGCTCCGCTTGCTAACCG
TCTAGGTATTGGTCAATTAAAGTGGGAAATTGAGGATTACTCTTTCCGCTACCAACAACCGGATACCTATAAAAAAATCG
CGAAACAACTTTCAGAACGAAGAATTGTTCGAGAGCAATACATCCATGATTTTGTCACTGATTTAAGTGATGAGATGAAA
GTATGCAATATCAATGCTGAAGTAAGCGGTAGGCCTAAACACATCTACAGCATTTGGCGAAAAATGCAGAAGAAAAGCTT
AGCCTTTGATGAGCTTTTTGATGTTCGAGCTGTTCGAATTATTGCTGATAAACTGCAAGATTGTTATGCGGCTTTAGGTG
TTGTTCATACCAAATTTAAACACCTACCAAGTGAGTTTGATGACTATGTGGCAAACCCAAAACCCAATGGTTATCAATCA
ATTCATACAGTTGTTCTTGGTCCTGAAGGTAAAACCATAGAAATTCAGATCCGTACCAAGGACATGCATGAAGACTCTGA
GTTAGGAGTTGCTGCTCACTGGAAATACAAAGAAGGTCCGGGTAGTAACAGCCGTAGTGGTTATGATGAAAAAATCACAT
GGTTACGTAAGTTACTTGATTGGCAAGAAGAGATGTCTGATTCGGGCGAAATGCTGGATGAACTTCGTAGCCAAGTATTT
GATGATCGTGTTTATGCCTTTACCCCTCGCGGTGATGTGGTCGATTTACCCATGGGAGCAACGCCACTCGACTTTGCCTA
TCATATTCACTCAGAAGTAGGACATCGTTGTATTGGTGCTAAGGTTGGTGGACGTATTGTTCCTTTCACGCATCAATTAT
CAATGGGTGATCAAGTTGAAATTATCACTCAAAAAGAACCAAACCCATCACGTGATTGGTTAAATCCAACATTGGGTTTT
GTTAACTCAAGTCGTGCTCGCGCTAAGATTAATGCGTGGTTTAGAAAGCAAAGTCGTGAGAAAAACCTAGACGCAGGTAA
AGAGATCCTTGAAGTTGAATTAGCTAAAATTGGTGGAACATTGAAAGATGCTGAGCGTTATGCTCTTAAGCGTTTCAATG
TAAATTCAACTGATGAGCTTTTTGCTGGGATTGGTAGTGGTGATCTTCGTATTAACCAGATCGTAAATCACATCAATGCG
CTAGTAAATAAACCAACGGCAGAGCAAGAAGATCAAAAGGCGCTTGAGCAACTTCAAGAATCAGAAGCAAAAGCGCAAGC
TCAGGCACAAGCACAGAGCAAACAACGTAAAGATGCAGTTGTTGTTGAAGGGGTTGATAACTTAATGACGCATTTAGCGC
GTTGTTGTCAGCCAATCCCGGGTGACCCAATTGCAGGGTATATTACACAAGGTCGTGGTATTTCAGTTCACCGCAGTGAC
TGTGATCAACTAAACGAGCTTCGTCATCATGCTTCAGAACGTATTATTGATACGGTATGGGGAAGTGGTTTTGTTGGCTC
TTATATCCTGACTTTACGTATTACAGCAATGGATCGAACTGGGCTGTTAAAGGATCTAACGACTCTATTTGCTAATGAAA
AAGTCAAAGTGTCAGGCATGAAAAGCCGTAATGATTACAAGAAGCAGTTAACCATCATGGACTTTGATCTTGAGGTCTTT
AATGCGGATATTGTTGGTCGATTGATTACACGAGTTGAACAAGTTAAAGATGTAATGGAAGCCATTCGCTTGTCATAA

Upstream 100 bases:

>100_bases
GCATTTAGAATCAATGGCATTGTTTGAACGTTAACTCACTCTTGAGTTCGTTAGGCGGGATCAAAAGAATTACAGATTAG
AGAATTAATAGGAAACGGAC

Downstream 100 bases:

>100_bases
TCGTTATAGAGAAAATAAATTAACTGTTTTATCAATAAGATAGATTAAAGCCGGATATTTTTTAAAATATTCGGCTTTTT
TATTGAAAAAAAATCTCAAG

Product: (p)ppGpp synthetase I/GTP pyrophosphokinase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MVAVRGAHLKENETFELAEWIDSLEQDKPTSKKLTEVYQHCQELVASHENGQLLLWRGREMIEILITLSMDKATLVAAQL
FPLVSSGLLDKETLTEHYSKEIEKLIEGVEEMDSIGQLNATVGPESASNQVDNIRRMLLAMVDDFRCVIIKLAERICNLR
EVKNEPDEVRRAAAKECNNIYAPLANRLGIGQLKWEIEDYSFRYQQPDTYKKIAKQLSERRIVREQYIHDFVTDLSDEMK
VCNINAEVSGRPKHIYSIWRKMQKKSLAFDELFDVRAVRIIADKLQDCYAALGVVHTKFKHLPSEFDDYVANPKPNGYQS
IHTVVLGPEGKTIEIQIRTKDMHEDSELGVAAHWKYKEGPGSNSRSGYDEKITWLRKLLDWQEEMSDSGEMLDELRSQVF
DDRVYAFTPRGDVVDLPMGATPLDFAYHIHSEVGHRCIGAKVGGRIVPFTHQLSMGDQVEIITQKEPNPSRDWLNPTLGF
VNSSRARAKINAWFRKQSREKNLDAGKEILEVELAKIGGTLKDAERYALKRFNVNSTDELFAGIGSGDLRINQIVNHINA
LVNKPTAEQEDQKALEQLQESEAKAQAQAQAQSKQRKDAVVVEGVDNLMTHLARCCQPIPGDPIAGYITQGRGISVHRSD
CDQLNELRHHASERIIDTVWGSGFVGSYILTLRITAMDRTGLLKDLTTLFANEKVKVSGMKSRNDYKKQLTIMDFDLEVF
NADIVGRLITRVEQVKDVMEAIRLS

Sequences:

>Translated_745_residues
MVAVRGAHLKENETFELAEWIDSLEQDKPTSKKLTEVYQHCQELVASHENGQLLLWRGREMIEILITLSMDKATLVAAQL
FPLVSSGLLDKETLTEHYSKEIEKLIEGVEEMDSIGQLNATVGPESASNQVDNIRRMLLAMVDDFRCVIIKLAERICNLR
EVKNEPDEVRRAAAKECNNIYAPLANRLGIGQLKWEIEDYSFRYQQPDTYKKIAKQLSERRIVREQYIHDFVTDLSDEMK
VCNINAEVSGRPKHIYSIWRKMQKKSLAFDELFDVRAVRIIADKLQDCYAALGVVHTKFKHLPSEFDDYVANPKPNGYQS
IHTVVLGPEGKTIEIQIRTKDMHEDSELGVAAHWKYKEGPGSNSRSGYDEKITWLRKLLDWQEEMSDSGEMLDELRSQVF
DDRVYAFTPRGDVVDLPMGATPLDFAYHIHSEVGHRCIGAKVGGRIVPFTHQLSMGDQVEIITQKEPNPSRDWLNPTLGF
VNSSRARAKINAWFRKQSREKNLDAGKEILEVELAKIGGTLKDAERYALKRFNVNSTDELFAGIGSGDLRINQIVNHINA
LVNKPTAEQEDQKALEQLQESEAKAQAQAQAQSKQRKDAVVVEGVDNLMTHLARCCQPIPGDPIAGYITQGRGISVHRSD
CDQLNELRHHASERIIDTVWGSGFVGSYILTLRITAMDRTGLLKDLTTLFANEKVKVSGMKSRNDYKKQLTIMDFDLEVF
NADIVGRLITRVEQVKDVMEAIRLS
>Mature_745_residues
MVAVRGAHLKENETFELAEWIDSLEQDKPTSKKLTEVYQHCQELVASHENGQLLLWRGREMIEILITLSMDKATLVAAQL
FPLVSSGLLDKETLTEHYSKEIEKLIEGVEEMDSIGQLNATVGPESASNQVDNIRRMLLAMVDDFRCVIIKLAERICNLR
EVKNEPDEVRRAAAKECNNIYAPLANRLGIGQLKWEIEDYSFRYQQPDTYKKIAKQLSERRIVREQYIHDFVTDLSDEMK
VCNINAEVSGRPKHIYSIWRKMQKKSLAFDELFDVRAVRIIADKLQDCYAALGVVHTKFKHLPSEFDDYVANPKPNGYQS
IHTVVLGPEGKTIEIQIRTKDMHEDSELGVAAHWKYKEGPGSNSRSGYDEKITWLRKLLDWQEEMSDSGEMLDELRSQVF
DDRVYAFTPRGDVVDLPMGATPLDFAYHIHSEVGHRCIGAKVGGRIVPFTHQLSMGDQVEIITQKEPNPSRDWLNPTLGF
VNSSRARAKINAWFRKQSREKNLDAGKEILEVELAKIGGTLKDAERYALKRFNVNSTDELFAGIGSGDLRINQIVNHINA
LVNKPTAEQEDQKALEQLQESEAKAQAQAQAQSKQRKDAVVVEGVDNLMTHLARCCQPIPGDPIAGYITQGRGISVHRSD
CDQLNELRHHASERIIDTVWGSGFVGSYILTLRITAMDRTGLLKDLTTLFANEKVKVSGMKSRNDYKKQLTIMDFDLEVF
NADIVGRLITRVEQVKDVMEAIRLS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=746, Percent_Identity=63.0026809651474, Blast_Score=993, Evalue=0.0,
Organism=Escherichia coli, GI1790082, Length=688, Percent_Identity=32.8488372093023, Blast_Score=385, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 84522; Mature: 84522

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVRGAHLKENETFELAEWIDSLEQDKPTSKKLTEVYQHCQELVASHENGQLLLWRGRE
CCEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
MIEILITLSMDKATLVAAQLFPLVSSGLLDKETLTEHYSKEIEKLIEGVEEMDSIGQLNA
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TVGPESASNQVDNIRRMLLAMVDDFRCVIIKLAERICNLREVKNEPDEVRRAAAKECNNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YAPLANRLGIGQLKWEIEDYSFRYQQPDTYKKIAKQLSERRIVREQYIHDFVTDLSDEMK
HHHHHHCCCCCEEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VCNINAEVSGRPKHIYSIWRKMQKKSLAFDELFDVRAVRIIADKLQDCYAALGVVHTKFK
EEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLPSEFDDYVANPKPNGYQSIHTVVLGPEGKTIEIQIRTKDMHEDSELGVAAHWKYKEGP
HCCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCC
GSNSRSGYDEKITWLRKLLDWQEEMSDSGEMLDELRSQVFDDRVYAFTPRGDVVDLPMGA
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCC
TPLDFAYHIHSEVGHRCIGAKVGGRIVPFTHQLSMGDQVEIITQKEPNPSRDWLNPTLGF
CCHHHHHHHHHHHCCEEECCCCCCEEECEEEECCCCCCEEEEECCCCCCCCHHCCCHHHH
VNSSRARAKINAWFRKQSREKNLDAGKEILEVELAKIGGTLKDAERYALKRFNVNSTDEL
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHH
FAGIGSGDLRINQIVNHINALVNKPTAEQEDQKALEQLQESEAKAQAQAQAQSKQRKDAV
HHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VVEGVDNLMTHLARCCQPIPGDPIAGYITQGRGISVHRSDCDQLNELRHHASERIIDTVW
EECCHHHHHHHHHHHCCCCCCCCCCHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHC
GSGFVGSYILTLRITAMDRTGLLKDLTTLFANEKVKVSGMKSRNDYKKQLTIMDFDLEVF
CCCHHHHEEEEEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHEEEEEEECHHHH
NADIVGRLITRVEQVKDVMEAIRLS
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVAVRGAHLKENETFELAEWIDSLEQDKPTSKKLTEVYQHCQELVASHENGQLLLWRGRE
CCEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
MIEILITLSMDKATLVAAQLFPLVSSGLLDKETLTEHYSKEIEKLIEGVEEMDSIGQLNA
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TVGPESASNQVDNIRRMLLAMVDDFRCVIIKLAERICNLREVKNEPDEVRRAAAKECNNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YAPLANRLGIGQLKWEIEDYSFRYQQPDTYKKIAKQLSERRIVREQYIHDFVTDLSDEMK
HHHHHHCCCCCEEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VCNINAEVSGRPKHIYSIWRKMQKKSLAFDELFDVRAVRIIADKLQDCYAALGVVHTKFK
EEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLPSEFDDYVANPKPNGYQSIHTVVLGPEGKTIEIQIRTKDMHEDSELGVAAHWKYKEGP
HCCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCC
GSNSRSGYDEKITWLRKLLDWQEEMSDSGEMLDELRSQVFDDRVYAFTPRGDVVDLPMGA
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCC
TPLDFAYHIHSEVGHRCIGAKVGGRIVPFTHQLSMGDQVEIITQKEPNPSRDWLNPTLGF
CCHHHHHHHHHHHCCEEECCCCCCEEECEEEECCCCCCEEEEECCCCCCCCHHCCCHHHH
VNSSRARAKINAWFRKQSREKNLDAGKEILEVELAKIGGTLKDAERYALKRFNVNSTDEL
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHH
FAGIGSGDLRINQIVNHINALVNKPTAEQEDQKALEQLQESEAKAQAQAQAQSKQRKDAV
HHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VVEGVDNLMTHLARCCQPIPGDPIAGYITQGRGISVHRSDCDQLNELRHHASERIIDTVW
EECCHHHHHHHHHHHCCCCCCCCCCHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHC
GSGFVGSYILTLRITAMDRTGLLKDLTTLFANEKVKVSGMKSRNDYKKQLTIMDFDLEVF
CCCHHHHEEEEEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHEEEEEEECHHHH
NADIVGRLITRVEQVKDVMEAIRLS
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7928955 [H]