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The map label for this gene is rpoS [H]

Identifier: 59712674

GI number: 59712674

Start: 2303271

End: 2304236

Strand: Reverse

Name: rpoS [H]

Synonym: VF_2067

Alternate gene names: 59712674

Gene position: 2304236-2303271 (Counterclockwise)

Preceding gene: 59712675

Following gene: 59712672

Centisome position: 79.52

GC content: 39.86

Gene sequence:

>966_bases
ATGAGTAAAAGCAATGCAGTAACTAAAGAAGTTGAAGAGTTCGATTTAGACACAATTAATGATGCTGAATTAACCAGTAT
TGAAGAAGAGTCTGAACGTCTTGAAGTAAGCAGTTCACAGAAAGTTATGGATGCAACGCAACTGTATCTTGGTGAAATTG
GCTTTTCTCCACTACTAACAGCAGAAGAAGAAGTTTATTTTAGTCGCTTAGCCTTAAAAGGTGATGAAGCCGCTCGAGCT
CGAATGATTGAGAGTAACCTTCGATTGGTTGTGAAAATTGCTCGTCGTTATAATCACCGTGGTCTTGCTTTATTGGATTT
GGTTGAAGAGGGAAATCTTGGCCTGATCCGAGCCGTTGAAAAGTTTGATCCAGAAAGAGGCTTCCGATTCTCTACTTACG
CCACATGGTGGATACGTCAAACCATTGAACGTGCCATTATGAACCAAACGCGTACCATTCGTTTACCTATTCATGTTGTA
AAAGAGCTTAACGTTTATTTGCGTGCAGCTCGTGAACTCTCTCAAAAGCTTGATCATGAACCAACACCAGAAGAAATAGC
TGAAAAGCTCGATAAGCCAGTGAGCGATGTTAGTCGAATGCTACGTTTAAATGAGCGTGTCGCTTCAGTCGACTCTATGG
TGGCTGGTGAATCAGATAAAGCGTTATTAGATATCCTTCCTGATACTAAAAATAGTGACCCAGAGTCAGAAACACAAAAG
GATGATATTAATACGTCATTGGTGGGCTGGTTAGGTCAATTAAACCCTAAACAAAAAGAAGTTGTTGCCCGTCGTTTTGG
TTTACTTGGTTATGAAGCTGCAACATTAGAACAAGTTGGTATTGAAATTGGTTTGACTCGTGAACGTGTAAGACAGATAC
AAGTAGAAGGCTTACGTCGTTTAAGAGAGATCATGACGAATCAAGGCTTGAATATGGAAACATTATTTGCAGTTGAAGAA
TACTAA

Upstream 100 bases:

>100_bases
CCGCTTTAGAGGGAAATCGGTAAATCCACAGAGGTATTTACCATAAAAGGATGACATAGCTGAGTTGTAATGTCTTGCTG
GCTCGCCATGGGGAGGCGCT

Downstream 100 bases:

>100_bases
CTCACTTTTTAATATCCGTTTTAGTTAGTAAAAGAGCTCTAGTTTATCTAGAGCTCTTTTTATTGATATAAACCTTTTAG
ATATGAATGAGAGAGGCGTA

Product: RNA polymerase, sigma-S (sigma-38) factor

Products: NA

Alternate protein names: Sigma-38 factor [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSKSNAVTKEVEEFDLDTINDAELTSIEEESERLEVSSSQKVMDATQLYLGEIGFSPLLTAEEEVYFSRLALKGDEAARA
RMIESNLRLVVKIARRYNHRGLALLDLVEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVV
KELNVYLRAARELSQKLDHEPTPEEIAEKLDKPVSDVSRMLRLNERVASVDSMVAGESDKALLDILPDTKNSDPESETQK
DDINTSLVGWLGQLNPKQKEVVARRFGLLGYEAATLEQVGIEIGLTRERVRQIQVEGLRRLREIMTNQGLNMETLFAVEE
Y

Sequences:

>Translated_321_residues
MSKSNAVTKEVEEFDLDTINDAELTSIEEESERLEVSSSQKVMDATQLYLGEIGFSPLLTAEEEVYFSRLALKGDEAARA
RMIESNLRLVVKIARRYNHRGLALLDLVEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVV
KELNVYLRAARELSQKLDHEPTPEEIAEKLDKPVSDVSRMLRLNERVASVDSMVAGESDKALLDILPDTKNSDPESETQK
DDINTSLVGWLGQLNPKQKEVVARRFGLLGYEAATLEQVGIEIGLTRERVRQIQVEGLRRLREIMTNQGLNMETLFAVEE
Y
>Mature_320_residues
SKSNAVTKEVEEFDLDTINDAELTSIEEESERLEVSSSQKVMDATQLYLGEIGFSPLLTAEEEVYFSRLALKGDEAARAR
MIESNLRLVVKIARRYNHRGLALLDLVEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVK
ELNVYLRAARELSQKLDHEPTPEEIAEKLDKPVSDVSRMLRLNERVASVDSMVAGESDKALLDILPDTKNSDPESETQKD
DINTSLVGWLGQLNPKQKEVVARRFGLLGYEAATLEQVGIEIGLTRERVRQIQVEGLRRLREIMTNQGLNMETLFAVEEY

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. May be required for the persistence of V.cholerae in aquatic habitats [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=287, Percent_Identity=79.4425087108014, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1789448, Length=236, Percent_Identity=40.2542372881356, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1789871, Length=266, Percent_Identity=27.0676691729323, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36608; Mature: 36477

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSNAVTKEVEEFDLDTINDAELTSIEEESERLEVSSSQKVMDATQLYLGEIGFSPLLT
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
AEEEVYFSRLALKGDEAARARMIESNLRLVVKIARRYNHRGLALLDLVEEGNLGLIRAVE
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHH
KFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARELSQKLDHE
HCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
PTPEEIAEKLDKPVSDVSRMLRLNERVASVDSMVAGESDKALLDILPDTKNSDPESETQK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHH
DDINTSLVGWLGQLNPKQKEVVARRFGLLGYEAATLEQVGIEIGLTRERVRQIQVEGLRR
HHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LREIMTNQGLNMETLFAVEEY
HHHHHHCCCCCHHEEEEEECC
>Mature Secondary Structure 
SKSNAVTKEVEEFDLDTINDAELTSIEEESERLEVSSSQKVMDATQLYLGEIGFSPLLT
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
AEEEVYFSRLALKGDEAARARMIESNLRLVVKIARRYNHRGLALLDLVEEGNLGLIRAVE
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHH
KFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARELSQKLDHE
HCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
PTPEEIAEKLDKPVSDVSRMLRLNERVASVDSMVAGESDKALLDILPDTKNSDPESETQK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHH
DDINTSLVGWLGQLNPKQKEVVARRFGLLGYEAATLEQVGIEIGLTRERVRQIQVEGLRR
HHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LREIMTNQGLNMETLFAVEEY
HHHHHHCCCCCHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9473029; 10952301 [H]