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The map label for this gene is pdxY

Identifier: 59712663

GI number: 59712663

Start: 2288397

End: 2289266

Strand: Reverse

Name: pdxY

Synonym: VF_2056

Alternate gene names: 59712663

Gene position: 2289266-2288397 (Counterclockwise)

Preceding gene: 59712664

Following gene: 59712662

Centisome position: 79.01

GC content: 40.57

Gene sequence:

>870_bases
ATGAAACGTATTCTTTCCATTCAGTCTCATGTGGTATTTGGTTGCGCAGGCAACAGTGCTGCGGTTTTTCCAATGCGAAG
AATGGGAATGGAAGTATGGCCTATTAATACGGTACAGTTTTCAAATCACACACAATATCAACAAGGTTGGAAAGGCATCG
CAATGCCTGCTGGCCACATTTCAGAATTAGTTGATGGTTTATCAGCAATTGAAGCGACTCAAGTTTGTGATGCCGTGTTA
AGTGGTTACTTAGGTTCAGCAGCGCAAGGTCAAGAGATTGTTACTGCGGTAAACAAAATCAAACAAGATAATCCAAACGC
AATTTACTTTTGTGATCCGGTAATGGGTCATCCTGAAAAAGGCTGCATTGTTGCTCCTGAAGTAGAAACCTTCTTTAAAG
AATCAGCTTTAAGTTCTGCGGATATTATCGCGCCTAATTTATTAGAACTAGAAAGCTTAACTGGCATGACAATTAACACC
TTAGATCAAGTGATTGAAGCGAATAATCAATTACTTGAGAAGGGTGTTAAAATGGTGGTGGTGAAACATTTAAGCCGTGC
TGGTATTCAAAAAGATCGCTTTGAAATGTTATTAACGACTGAAGATGGAAGCTACCACGTTTCTCGTCCTTTATATGATT
TTGATGCAAAGCGTCAACCCGTAGGCGCAGGTGACTTAATTAGCGGTGTGATGCTTGCGAACTTAATGGCAGGTTACTCT
CCAATTGATGCGTTTGAACGTACTAATGCAGCCGTTGATTCTGTTATGCAAGAAACCTTCAATCGCGGCGCGTATGAACT
GCAACTTATCGCATCACAAGAACGTTTTAATGCTCCTGAAATTATCGTAAAAGCAGAAAAAGTCGCGTAA

Upstream 100 bases:

>100_bases
GTGAAAGTACTAAAACAAAATTAAGCCCTAGGGTTTTGATGGAATATCGTTTATTTTAGGGCACGGAAGCCCTTACTCTA
TTTTAAATTATGGTGATGTT

Downstream 100 bases:

>100_bases
TTAGCTCGATATAAAAATCACAAATAAAATAGAAGAGCCCATGATGTGTAATATGCATATCATGGGCTTTTTGTGTATTT
TTAGTTTAAAAAATGAATTT

Product: pyridoxal kinase 2/pyridoxine kinase

Products: NA

Alternate protein names: PM kinase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKRILSIQSHVVFGCAGNSAAVFPMRRMGMEVWPINTVQFSNHTQYQQGWKGIAMPAGHISELVDGLSAIEATQVCDAVL
SGYLGSAAQGQEIVTAVNKIKQDNPNAIYFCDPVMGHPEKGCIVAPEVETFFKESALSSADIIAPNLLELESLTGMTINT
LDQVIEANNQLLEKGVKMVVVKHLSRAGIQKDRFEMLLTTEDGSYHVSRPLYDFDAKRQPVGAGDLISGVMLANLMAGYS
PIDAFERTNAAVDSVMQETFNRGAYELQLIASQERFNAPEIIVKAEKVA

Sequences:

>Translated_289_residues
MKRILSIQSHVVFGCAGNSAAVFPMRRMGMEVWPINTVQFSNHTQYQQGWKGIAMPAGHISELVDGLSAIEATQVCDAVL
SGYLGSAAQGQEIVTAVNKIKQDNPNAIYFCDPVMGHPEKGCIVAPEVETFFKESALSSADIIAPNLLELESLTGMTINT
LDQVIEANNQLLEKGVKMVVVKHLSRAGIQKDRFEMLLTTEDGSYHVSRPLYDFDAKRQPVGAGDLISGVMLANLMAGYS
PIDAFERTNAAVDSVMQETFNRGAYELQLIASQERFNAPEIIVKAEKVA
>Mature_289_residues
MKRILSIQSHVVFGCAGNSAAVFPMRRMGMEVWPINTVQFSNHTQYQQGWKGIAMPAGHISELVDGLSAIEATQVCDAVL
SGYLGSAAQGQEIVTAVNKIKQDNPNAIYFCDPVMGHPEKGCIVAPEVETFFKESALSSADIIAPNLLELESLTGMTINT
LDQVIEANNQLLEKGVKMVVVKHLSRAGIQKDRFEMLLTTEDGSYHVSRPLYDFDAKRQPVGAGDLISGVMLANLMAGYS
PIDAFERTNAAVDSVMQETFNRGAYELQLIASQERFNAPEIIVKAEKVA

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxamine

COG id: COG2240

COG function: function code H; Pyridoxal/pyridoxine/pyridoxamine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyridoxine kinase family

Homologues:

Organism=Homo sapiens, GI4505701, Length=308, Percent_Identity=32.1428571428571, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1787924, Length=288, Percent_Identity=54.5138888888889, Blast_Score=327, Evalue=7e-91,
Organism=Escherichia coli, GI1788758, Length=267, Percent_Identity=31.4606741573034, Blast_Score=122, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17507759, Length=305, Percent_Identity=33.1147540983607, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17507757, Length=315, Percent_Identity=32.3809523809524, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320806, Length=237, Percent_Identity=35.0210970464135, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324354, Length=281, Percent_Identity=29.5373665480427, Blast_Score=92, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45553007, Length=308, Percent_Identity=30.8441558441558, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXY_VIBF1 (Q5E345)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205439.1
- HSSP:   P77150
- ProteinModelPortal:   Q5E345
- SMR:   Q5E345
- STRING:   Q5E345
- GeneID:   3279161
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2056
- NMPDR:   fig|312309.3.peg.2056
- eggNOG:   COG2240
- HOGENOM:   HBG661459
- OMA:   CPNQLEL
- PhylomeDB:   Q5E345
- ProtClustDB:   CLSK2396217
- BioCyc:   VFIS312309:VF2056-MONOMER
- HAMAP:   MF_01639
- InterPro:   IPR013749
- InterPro:   IPR004625
- TIGRFAMs:   TIGR00687

Pfam domain/function: PF08543 Phos_pyr_kin

EC number: =2.7.1.35

Molecular weight: Translated: 31510; Mature: 31510

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: BINDING 9-9 BINDING 44-44 BINDING 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILSIQSHVVFGCAGNSAAVFPMRRMGMEVWPINTVQFSNHTQYQQGWKGIAMPAGHI
CCCHHEECCEEEEEECCCCCEECHHHHCCCEEECCCEEEECCCCCHHHCCCCCCCCHHHH
SELVDGLSAIEATQVCDAVLSGYLGSAAQGQEIVTAVNKIKQDNPNAIYFCDPVMGHPEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
GCIVAPEVETFFKESALSSADIIAPNLLELESLTGMTINTLDQVIEANNQLLEKGVKMVV
CCEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VKHLSRAGIQKDRFEMLLTTEDGSYHVSRPLYDFDAKRQPVGAGDLISGVMLANLMAGYS
HHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PIDAFERTNAAVDSVMQETFNRGAYELQLIASQERFNAPEIIVKAEKVA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCEEEEEECCCC
>Mature Secondary Structure
MKRILSIQSHVVFGCAGNSAAVFPMRRMGMEVWPINTVQFSNHTQYQQGWKGIAMPAGHI
CCCHHEECCEEEEEECCCCCEECHHHHCCCEEECCCEEEECCCCCHHHCCCCCCCCHHHH
SELVDGLSAIEATQVCDAVLSGYLGSAAQGQEIVTAVNKIKQDNPNAIYFCDPVMGHPEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
GCIVAPEVETFFKESALSSADIIAPNLLELESLTGMTINTLDQVIEANNQLLEKGVKMVV
CCEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VKHLSRAGIQKDRFEMLLTTEDGSYHVSRPLYDFDAKRQPVGAGDLISGVMLANLMAGYS
HHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PIDAFERTNAAVDSVMQETFNRGAYELQLIASQERFNAPEIIVKAEKVA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA