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The map label for this gene is aceA [H]

Identifier: 59712579

GI number: 59712579

Start: 2203631

End: 2204959

Strand: Reverse

Name: aceA [H]

Synonym: VF_1972

Alternate gene names: 59712579

Gene position: 2204959-2203631 (Counterclockwise)

Preceding gene: 59712580

Following gene: 59712577

Centisome position: 76.1

GC content: 43.12

Gene sequence:

>1329_bases
ATGACGACATTTAAACCACAAACTCGCCAACAGCAAATTGATGCACTGGAAAAAGATTGGGCTGAAAATCCGCGTTGGGA
TGGGGTAACTCGTCCTTATACGGCTGAAGAAGTAATTAATCTTCGTGGATCTTTTGCTCCAGAAAATACCATTGCACAAA
AGGGTGCAGACAGATTGTGGGACCTAGTCAATGGTTCTTCTAAAAAAGGGTATGTGAATTGTCTTGGTGCGTTGACTGGT
GGACAAGCAGTCCAGCAAGCAAAAGCAGGAATTGAGGCTATTTACCTGTCAGGTTGGCAAGTAGCTGCGGATAATAATAC
CGCATCAACCATGTACCCAGACCAATCTTTGTATCCGGTAGATTCAGTGCCATCGGTAGTGAAACGAATTAATAATGCAT
TTCGTCGAGCAGACCAAATTCAATGGTCTACAGGTGGAACTCCTGAAGAGGGAATTGATTACTTTTTGCCTATTGTAGCG
GATGCAGAAGCGGGATTTGGTGGTGTGCTTAATGGTTATGAATTGATGCGTAATATGATTGAAGCCGGTGCTGCAGGCGT
TCACTTTGAAGATCAATTAGCGTCAGTGAAAAAGTGCGGTCATATGGGGGGGAAGGTGTTGGTACCAACGCAAGAAGCGG
TGCAAAAGTTGGTAGCAGCTCGTCTTGCTGCTGATGTAGCCGGAACCCGAACACTTGTAATTGCTAGAACCGATGCTAAT
GCGGCTGATTTGCTGACATCAGATTGTGATCCATACGATCAAGAGTTCATTGAAGGTGAGCGAACACCTGAAGGTTTTTA
TAAAGTAACGGCGGGGATTGATCAGGCAATATCAAGAGGATTGGCTTATTCTCCTTATGCGGATCTTGTGTGGTGTGAAA
CGGCGACACCTTGTTTGGAAGAAGCACGTAAATTTGCAGAAGCAATTCATGCAAAATACCCAAATCAGCTATTAGCTTAT
AACTGTTCTCCATCATTTAACTGGGAGAAAAATCTGGATTCAGAGACGATTGCGAAATTCCAACAAGAGTTGGCGGATAT
GGGATATAAGTACCAATTCATCACGCTCGCTGGTATTCATAATATGTGGTTTAACATGTTTGAATTGGCTCACTCTTATG
CTCAAGGTGAAGGCATGCGTCACTATGTTGAAATGGTTCAACGTAAAGAATTTGAAGCAGCAGAGAAAGGCTATACTTTT
GTAGCGCACCAACAAGAAGTTGGTACCGGATATTTTGATAAAATGACAAATACCATTCAAGGTGGTCAATCATCAGTAAC
CGCACTTACGGGATCGACAGAAGAAGAGCAATTTAAAACCAGTGCATAG

Upstream 100 bases:

>100_bases
CTCAAGCTGCAAAGCTGATGGAGCAACTAACCACCAGTGATCAACTTACGGACTTTTTAACACTACCTGGCTACCAATAC
CTATAAGAGAGGGAACGATT

Downstream 100 bases:

>100_bases
GAATTAAGTAATTAATAATTAAAATAAATAGCACTAGCTGGATTTGAAGGGAGAATGGAGTCTCCCTTTCTTTTTGGCTG
TTATTCGTCAAGCTCTAATG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTTFKPQTRQQQIDALEKDWAENPRWDGVTRPYTAEEVINLRGSFAPENTIAQKGADRLWDLVNGSSKKGYVNCLGALTG
GQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNAFRRADQIQWSTGGTPEEGIDYFLPIVA
DAEAGFGGVLNGYELMRNMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTRTLVIARTDAN
AADLLTSDCDPYDQEFIEGERTPEGFYKVTAGIDQAISRGLAYSPYADLVWCETATPCLEEARKFAEAIHAKYPNQLLAY
NCSPSFNWEKNLDSETIAKFQQELADMGYKYQFITLAGIHNMWFNMFELAHSYAQGEGMRHYVEMVQRKEFEAAEKGYTF
VAHQQEVGTGYFDKMTNTIQGGQSSVTALTGSTEEEQFKTSA

Sequences:

>Translated_442_residues
MTTFKPQTRQQQIDALEKDWAENPRWDGVTRPYTAEEVINLRGSFAPENTIAQKGADRLWDLVNGSSKKGYVNCLGALTG
GQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNAFRRADQIQWSTGGTPEEGIDYFLPIVA
DAEAGFGGVLNGYELMRNMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTRTLVIARTDAN
AADLLTSDCDPYDQEFIEGERTPEGFYKVTAGIDQAISRGLAYSPYADLVWCETATPCLEEARKFAEAIHAKYPNQLLAY
NCSPSFNWEKNLDSETIAKFQQELADMGYKYQFITLAGIHNMWFNMFELAHSYAQGEGMRHYVEMVQRKEFEAAEKGYTF
VAHQQEVGTGYFDKMTNTIQGGQSSVTALTGSTEEEQFKTSA
>Mature_441_residues
TTFKPQTRQQQIDALEKDWAENPRWDGVTRPYTAEEVINLRGSFAPENTIAQKGADRLWDLVNGSSKKGYVNCLGALTGG
QAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPVDSVPSVVKRINNAFRRADQIQWSTGGTPEEGIDYFLPIVAD
AEAGFGGVLNGYELMRNMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTRTLVIARTDANA
ADLLTSDCDPYDQEFIEGERTPEGFYKVTAGIDQAISRGLAYSPYADLVWCETATPCLEEARKFAEAIHAKYPNQLLAYN
CSPSFNWEKNLDSETIAKFQQELADMGYKYQFITLAGIHNMWFNMFELAHSYAQGEGMRHYVEMVQRKEFEAAEKGYTFV
AHQQEVGTGYFDKMTNTIQGGQSSVTALTGSTEEEQFKTSA

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=434, Percent_Identity=76.4976958525346, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=267, Percent_Identity=28.4644194756554, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17561814, Length=421, Percent_Identity=54.8693586698337, Blast_Score=469, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71982926, Length=421, Percent_Identity=54.8693586698337, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6320908, Length=304, Percent_Identity=36.5131578947368, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6325263, Length=251, Percent_Identity=37.0517928286853, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48708; Mature: 48577

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFKPQTRQQQIDALEKDWAENPRWDGVTRPYTAEEVINLRGSFAPENTIAQKGADRLW
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
DLVNGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPV
HHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCC
DSVPSVVKRINNAFRRADQIQWSTGGTPEEGIDYFLPIVADAEAGFGGVLNGYELMRNMI
HHHHHHHHHHHHHHHHHHCEEECCCCCCHHCHHHHHHHEECCCCCCCCCHHHHHHHHHHH
EAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTRTLVIARTDAN
HHCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
AADLLTSDCDPYDQEFIEGERTPEGFYKVTAGIDQAISRGLAYSPYADLVWCETATPCLE
HHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHH
EARKFAEAIHAKYPNQLLAYNCSPSFNWEKNLDSETIAKFQQELADMGYKYQFITLAGIH
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHH
NMWFNMFELAHSYAQGEGMRHYVEMVQRKEFEAAEKGYTFVAHQQEVGTGYFDKMTNTIQ
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHHHHC
GGQSSVTALTGSTEEEQFKTSA
CCCCCEEEEECCCHHHHHCCCC
>Mature Secondary Structure 
TTFKPQTRQQQIDALEKDWAENPRWDGVTRPYTAEEVINLRGSFAPENTIAQKGADRLW
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
DLVNGSSKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTASTMYPDQSLYPV
HHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCC
DSVPSVVKRINNAFRRADQIQWSTGGTPEEGIDYFLPIVADAEAGFGGVLNGYELMRNMI
HHHHHHHHHHHHHHHHHHCEEECCCCCCHHCHHHHHHHEECCCCCCCCCHHHHHHHHHHH
EAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTRTLVIARTDAN
HHCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
AADLLTSDCDPYDQEFIEGERTPEGFYKVTAGIDQAISRGLAYSPYADLVWCETATPCLE
HHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHH
EARKFAEAIHAKYPNQLLAYNCSPSFNWEKNLDSETIAKFQQELADMGYKYQFITLAGIH
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHH
NMWFNMFELAHSYAQGEGMRHYVEMVQRKEFEAAEKGYTFVAHQQEVGTGYFDKMTNTIQ
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHHHHC
GGQSSVTALTGSTEEEQFKTSA
CCCCCEEEEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]