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The map label for this gene is queA [H]

Identifier: 59712577

GI number: 59712577

Start: 2201822

End: 2202874

Strand: Reverse

Name: queA [H]

Synonym: VF_1970

Alternate gene names: 59712577

Gene position: 2202874-2201822 (Counterclockwise)

Preceding gene: 59712579

Following gene: 59712576

Centisome position: 76.03

GC content: 41.31

Gene sequence:

>1053_bases
ATGCAAGTATCCGATTTCCATTTTGAATTACCTGATGAACTTATTGCGCGTTATCCGCAACCTGAGCGTACAGCAAGCCG
ACTATTACAATTGAATGGCAATAGTGGCGAACTTAATGATGGGCAGTTTACTGATATTCTTGATTTAGTTCAGGCTGGTG
ATTTATTGGTATTCAACAATACTCGAGTGATCCCTGCTCGTATGTTCGGTATGAAGGCTTCTGGTGGTAAATTAGAAGTA
TTGGTTGAACGTGTTCTTGATGAACATTCAGTTTTGGCTCATGTACGTTGTTCTAAATCGCCAAAACCAGGCACCATGCT
TTTATTGGGTGAAAACCAAGAACATGAAGCTGAAATGGTTGCTCGTCACGATACCTTGTTTGAGATTCGTTTTACTTCAG
ATAAGAAAGTCTTAGATATTTTAGATGAAATTGGCCACATGCCACTTCCTCCTTATATCGATCGTCCAGATGAAGATGCG
GATAAAGAACGCTACCAAACGGTTTATAACAAAAAGCCTGGTGCTGTAGCAGCTCCAACCGCTGGTTTACACTTTGATAC
AGAGATTTTAGAGAAAATGAAGGCAAAAGGGGTAGAATTTGCCTACGTAACCCTTCATGTTGGTGCTGGTACATTCCAGC
CCGTTCGTGTGGACAATATTTTAGAGCACCACATGCACTCTGAATACGCAGAAGTTTCCCAAGAAGTTATCGATGCTATC
AACGCAACAAAAGCGCGTGGTGGCCGAGTAGTTTCTGTGGGTACTACATCAGTACGTTCTTTAGAAAGTGCTGCGCAGCA
TGCCTTAAAACAAGGTACAGAGTTAGCGCCGTTTTTTGATGATACTGAAATTTTCATCTACCCAGGTTACGAATTTCAAG
TGGTTGACGCTTTGGTGACAAACTTCCACTTACCTGAGTCTACACTTATCATGCTAGTAAGTGCATTTGCAGGCTACGAG
AATACAATGAAAGCGTATGAGCAAGCGGTAAATAATAAATATCGTTTCTTTAGTTACGGTGATGCAATGTTCATCACGAA
AAAAACAGCCTAG

Upstream 100 bases:

>100_bases
TATTTTTTGAAAAAGTGAGAGGTATATAGCTTCATCCCTTGCTATTGCTCTGGGCGTGAATATACTACTCTGTCTGCCAT
TTTTGATGAAGAAGTAAACC

Downstream 100 bases:

>100_bases
TTATTCTTGTTTTAGCCACGGTTAGTTTATTATGGCTGGTAAATAATTATTCATTTCACTCACGGAGCCAGTGAAATATT
TAGGAAAACAGGACGTTTTC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MQVSDFHFELPDELIARYPQPERTASRLLQLNGNSGELNDGQFTDILDLVQAGDLLVFNNTRVIPARMFGMKASGGKLEV
LVERVLDEHSVLAHVRCSKSPKPGTMLLLGENQEHEAEMVARHDTLFEIRFTSDKKVLDILDEIGHMPLPPYIDRPDEDA
DKERYQTVYNKKPGAVAAPTAGLHFDTEILEKMKAKGVEFAYVTLHVGAGTFQPVRVDNILEHHMHSEYAEVSQEVIDAI
NATKARGGRVVSVGTTSVRSLESAAQHALKQGTELAPFFDDTEIFIYPGYEFQVVDALVTNFHLPESTLIMLVSAFAGYE
NTMKAYEQAVNNKYRFFSYGDAMFITKKTA

Sequences:

>Translated_350_residues
MQVSDFHFELPDELIARYPQPERTASRLLQLNGNSGELNDGQFTDILDLVQAGDLLVFNNTRVIPARMFGMKASGGKLEV
LVERVLDEHSVLAHVRCSKSPKPGTMLLLGENQEHEAEMVARHDTLFEIRFTSDKKVLDILDEIGHMPLPPYIDRPDEDA
DKERYQTVYNKKPGAVAAPTAGLHFDTEILEKMKAKGVEFAYVTLHVGAGTFQPVRVDNILEHHMHSEYAEVSQEVIDAI
NATKARGGRVVSVGTTSVRSLESAAQHALKQGTELAPFFDDTEIFIYPGYEFQVVDALVTNFHLPESTLIMLVSAFAGYE
NTMKAYEQAVNNKYRFFSYGDAMFITKKTA
>Mature_350_residues
MQVSDFHFELPDELIARYPQPERTASRLLQLNGNSGELNDGQFTDILDLVQAGDLLVFNNTRVIPARMFGMKASGGKLEV
LVERVLDEHSVLAHVRCSKSPKPGTMLLLGENQEHEAEMVARHDTLFEIRFTSDKKVLDILDEIGHMPLPPYIDRPDEDA
DKERYQTVYNKKPGAVAAPTAGLHFDTEILEKMKAKGVEFAYVTLHVGAGTFQPVRVDNILEHHMHSEYAEVSQEVIDAI
NATKARGGRVVSVGTTSVRSLESAAQHALKQGTELAPFFDDTEIFIYPGYEFQVVDALVTNFHLPESTLIMLVSAFAGYE
NTMKAYEQAVNNKYRFFSYGDAMFITKKTA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=71.3872832369942, Blast_Score=518, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39143; Mature: 39143

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSDFHFELPDELIARYPQPERTASRLLQLNGNSGELNDGQFTDILDLVQAGDLLVFNN
CCCCCCCCCCCHHHHHCCCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHCCCEEEECC
TRVIPARMFGMKASGGKLEVLVERVLDEHSVLAHVRCSKSPKPGTMLLLGENQEHEAEMV
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCHHHHHH
ARHDTLFEIRFTSDKKVLDILDEIGHMPLPPYIDRPDEDADKERYQTVYNKKPGAVAAPT
HHCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCC
AGLHFDTEILEKMKAKGVEFAYVTLHVGAGTFQPVRVDNILEHHMHSEYAEVSQEVIDAI
CCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NATKARGGRVVSVGTTSVRSLESAAQHALKQGTELAPFFDDTEIFIYPGYEFQVVDALVT
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEHHHHHHH
NFHLPESTLIMLVSAFAGYENTMKAYEQAVNNKYRFFSYGDAMFITKKTA
HCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCEEEEEECCEEEEEECCC
>Mature Secondary Structure
MQVSDFHFELPDELIARYPQPERTASRLLQLNGNSGELNDGQFTDILDLVQAGDLLVFNN
CCCCCCCCCCCHHHHHCCCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHCCCEEEECC
TRVIPARMFGMKASGGKLEVLVERVLDEHSVLAHVRCSKSPKPGTMLLLGENQEHEAEMV
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCHHHHHH
ARHDTLFEIRFTSDKKVLDILDEIGHMPLPPYIDRPDEDADKERYQTVYNKKPGAVAAPT
HHCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCC
AGLHFDTEILEKMKAKGVEFAYVTLHVGAGTFQPVRVDNILEHHMHSEYAEVSQEVIDAI
CCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NATKARGGRVVSVGTTSVRSLESAAQHALKQGTELAPFFDDTEIFIYPGYEFQVVDALVT
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEHHHHHHH
NFHLPESTLIMLVSAFAGYENTMKAYEQAVNNKYRFFSYGDAMFITKKTA
HCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCEEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA