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The map label for this gene is tgt [H]

Identifier: 59712576

GI number: 59712576

Start: 2200481

End: 2201608

Strand: Reverse

Name: tgt [H]

Synonym: VF_1969

Alternate gene names: 59712576

Gene position: 2201608-2200481 (Counterclockwise)

Preceding gene: 59712577

Following gene: 59712575

Centisome position: 75.98

GC content: 41.67

Gene sequence:

>1128_bases
GTGAAATACGAACTGATCAAAAAACAAGGTCGCGCTCGTCGTGGTCAACTTCAATTTGACCGTGGTACGGTTGAAACTCC
TGCGTTCATGCCTGTTGGTACTTACGGTACTGTTAAAGGTATGACTCCTGAAGAAGTAAAAGGCACCGGTGCTGAAATCT
TACTAGGTAACACGTTCCATTTATGGTTACGCCCAGGTCAAGAGATCATGAAATTACACGGTGATTTACACGATTTCATG
AACTGGAAAGGTCCAATTTTAACGGATTCAGGTGGTTTCCAAGTATTTAGTCTTGGTAAAACGCGTAAGATCACTGAAGA
GGGTGTTCATTTCCGTAGCCCTGTAAATGGTGACAAGATCTTTATGGATGCAGAAAAATCAATGCAAATCCAATACGATC
TTGGTTCTGACGTGGTTATGATTTTTGATGAATGTACTCCATACCCAGCAACTCACGATGAAGCTCGTATTTCAATGGAA
CGTTCAATCCGTTGGGCTGAGCGTAGCCGTAATGAGTTCGATCGCCAAGAAAACCCAAATGCATTGTTTGGTATTGTTCA
AGGTGGTGTGTACGAAGATCTACGTGATGTGTCTGTTGAAGCATTAACTAAGATTGGTTTTGATGGTTATGCCGTTGGTG
GTCTAGCTGTTGGTGAGCCAAAAGAAGACATGCACCGCATTTTAGAGCATACATGTCCACAATTACCAGAAGATAAGCCA
CGATACCTAATGGGTGTTGGTAAACCAGAAGATTTGGTTGAAGGTGTTCGTCGCGGTATTGATATGTTTGACTGTGTAAT
GCCAACACGTAACGCACGTAATGGTCACTTATTTGTGACTGGTGGTGTGATCAAGATCCGTAATGCGAAACATAAAACAG
ATACAACACCACTAGATCCTGAGTGTGATTGTTATACTTGTCAAAACTACAGCAAGTCTTACCTACATCACTTAGACCGT
TGTAACGAAATTTTAGGTGCTCGTTTAAATACGATTCATAACTTACGTTATTACCAACGTATTATGGCGAGCATTCGTAA
GGCGTTGGAAGAAGATCGTTTTGAGCAGTTCGTAGAAGAATTCTACGCACGTCGTGATCGTGAAGTTCCACCATTAAAAG
ACCTTTAA

Upstream 100 bases:

>100_bases
GCTTTATCAGTTTACATGGAGGTGCCGCATTAACTTGTAGTTATTAATGTAGCAAATTAAACAAGTCGTCAGATTGTTTC
TCTGGCTTATTGGAGGTTTC

Downstream 100 bases:

>100_bases
AAGCAGAGTAATCACCCATATATCTAATGAGATATGTGTATGGTCACATTTTAGAAACTGGAAATTAGAAACTTAGATAC
GAGGACGTTATTAAATGAGT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKYELIKKQGRARRGQLQFDRGTVETPAFMPVGTYGTVKGMTPEEVKGTGAEILLGNTFHLWLRPGQEIMKLHGDLHDFM
NWKGPILTDSGGFQVFSLGKTRKITEEGVHFRSPVNGDKIFMDAEKSMQIQYDLGSDVVMIFDECTPYPATHDEARISME
RSIRWAERSRNEFDRQENPNALFGIVQGGVYEDLRDVSVEALTKIGFDGYAVGGLAVGEPKEDMHRILEHTCPQLPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAKHKTDTTPLDPECDCYTCQNYSKSYLHHLDR
CNEILGARLNTIHNLRYYQRIMASIRKALEEDRFEQFVEEFYARRDREVPPLKDL

Sequences:

>Translated_375_residues
MKYELIKKQGRARRGQLQFDRGTVETPAFMPVGTYGTVKGMTPEEVKGTGAEILLGNTFHLWLRPGQEIMKLHGDLHDFM
NWKGPILTDSGGFQVFSLGKTRKITEEGVHFRSPVNGDKIFMDAEKSMQIQYDLGSDVVMIFDECTPYPATHDEARISME
RSIRWAERSRNEFDRQENPNALFGIVQGGVYEDLRDVSVEALTKIGFDGYAVGGLAVGEPKEDMHRILEHTCPQLPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAKHKTDTTPLDPECDCYTCQNYSKSYLHHLDR
CNEILGARLNTIHNLRYYQRIMASIRKALEEDRFEQFVEEFYARRDREVPPLKDL
>Mature_375_residues
MKYELIKKQGRARRGQLQFDRGTVETPAFMPVGTYGTVKGMTPEEVKGTGAEILLGNTFHLWLRPGQEIMKLHGDLHDFM
NWKGPILTDSGGFQVFSLGKTRKITEEGVHFRSPVNGDKIFMDAEKSMQIQYDLGSDVVMIFDECTPYPATHDEARISME
RSIRWAERSRNEFDRQENPNALFGIVQGGVYEDLRDVSVEALTKIGFDGYAVGGLAVGEPKEDMHRILEHTCPQLPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAKHKTDTTPLDPECDCYTCQNYSKSYLHHLDR
CNEILGARLNTIHNLRYYQRIMASIRKALEEDRFEQFVEEFYARRDREVPPLKDL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=354, Percent_Identity=45.4802259887006, Blast_Score=323, Evalue=1e-88,
Organism=Escherichia coli, GI1786607, Length=373, Percent_Identity=80.9651474530831, Blast_Score=649, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=42.2651933701657, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI25146502, Length=252, Percent_Identity=28.968253968254, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19920510, Length=361, Percent_Identity=45.7063711911357, Blast_Score=320, Evalue=8e-88,
Organism=Drosophila melanogaster, GI21355311, Length=163, Percent_Identity=34.3558282208589, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42912; Mature: 42912

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYELIKKQGRARRGQLQFDRGTVETPAFMPVGTYGTVKGMTPEEVKGTGAEILLGNTFH
CCHHHHHHHCCCCCCCEEECCCCCCCCCCEECCCCCCCCCCCHHHHCCCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMNWKGPILTDSGGFQVFSLGKTRKITEEGVHFRSPVNGDKI
EEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCHHHHHCCCEECCCCCCCEE
FMDAEKSMQIQYDLGSDVVMIFDECTPYPATHDEARISMERSIRWAERSRNEFDRQENPN
EEECCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
ALFGIVQGGVYEDLRDVSVEALTKIGFDGYAVGGLAVGEPKEDMHRILEHTCPQLPEDKP
EEEEEECCCHHHHHHHHHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAKHKTDTTPLDP
CEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
ECDCYTCQNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRIMASIRKALEEDRFEQFVEE
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYARRDREVPPLKDL
HHHHCCCCCCCCCCC
>Mature Secondary Structure
MKYELIKKQGRARRGQLQFDRGTVETPAFMPVGTYGTVKGMTPEEVKGTGAEILLGNTFH
CCHHHHHHHCCCCCCCEEECCCCCCCCCCEECCCCCCCCCCCHHHHCCCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMNWKGPILTDSGGFQVFSLGKTRKITEEGVHFRSPVNGDKI
EEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCHHHHHCCCEECCCCCCCEE
FMDAEKSMQIQYDLGSDVVMIFDECTPYPATHDEARISMERSIRWAERSRNEFDRQENPN
EEECCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
ALFGIVQGGVYEDLRDVSVEALTKIGFDGYAVGGLAVGEPKEDMHRILEHTCPQLPEDKP
EEEEEECCCHHHHHHHHHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAKHKTDTTPLDP
CEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCC
ECDCYTCQNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRIMASIRKALEEDRFEQFVEE
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYARRDREVPPLKDL
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA