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The map label for this gene is pyrH

Identifier: 59712567

GI number: 59712567

Start: 2190131

End: 2190862

Strand: Reverse

Name: pyrH

Synonym: VF_1960

Alternate gene names: 59712567

Gene position: 2190862-2190131 (Counterclockwise)

Preceding gene: 59712568

Following gene: 59712566

Centisome position: 75.61

GC content: 43.58

Gene sequence:

>732_bases
ATGACGACGAACCCGAAACCAGTATATCAGCGTATTCTATTAAAACTTAGTGGTGAAGCACTTCAAGGCGAAGAGGGTTT
TGGTATTGACCCTGCAACGCTTGATCGTATGGCTCAAGAAGTAAAAGAGCTGATCGAGCTTGGTGTTCAAGTTGGTGTTG
TTATCGGTGGTGGTAACCTTTTCCGTGGTGCAGGTCTTGCAGAAGCAGGCATGAACCGTGTAGTAGGTGATCACATGGGT
ATGCTAGCGACAGTAATGAATGGCTTAGCGATGCGTGATGCACTACACCGTGCATACGTAAATGCTCGAGTAATGTCAGC
TATCCCTCTTAAAGGTGTATGTGACGATTACAACTGGGCTGATGCTATCGCTCAATTACGTCAAGGCCGTGTGGTTATTT
TCTCTGCTGGTACAGGTAACCCATTCTTTACAACAGACTCTGCTGCATGTCTACGTGGTATTGAAATTGAAGCTGATGTT
GTTTTAAAAGCAACAAAAGTAGATGGTGTATTTACTGCTGATCCAGTATCTAACCCTGATGCAGAATTGTATGATAAACT
GTCTTACAGCAGCGTTCTTGAAAAAGAATTAAAAGTGATGGACTTAGCAGCGTTTACTCTTGCTCGTGACCACAACATGC
CTATTCGTGTATTTAACATGAACAAGCCTGGTGCATTACGTCGTGTAGTTATGGGTGAAACTGAAGGCACACTGATCAGC
AACGCTGAATAA

Upstream 100 bases:

>100_bases
TGAATAGCTGTTACCTTTTACTATTGAATTATCAATAGAGGATAATCCAGTACTAAATGGTGACCGTTATTTTGACAACT
CTAGTGAAAAGGTAAAATCC

Downstream 100 bases:

>100_bases
TTCTCCTACGAATCAAAAATAATCAAGGTGATCTTGTGATTAACGAAATTAAAAAAGATGCGCAAGAGCGTATGGAAAAA
AGTGTTGAAGCACTGAAAAA

Product: uridylate kinase

Products: NA

Alternate protein names: UK; Uridine monophosphate kinase; UMP kinase; UMPK

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTTNPKPVYQRILLKLSGEALQGEEGFGIDPATLDRMAQEVKELIELGVQVGVVIGGGNLFRGAGLAEAGMNRVVGDHMG
MLATVMNGLAMRDALHRAYVNARVMSAIPLKGVCDDYNWADAIAQLRQGRVVIFSAGTGNPFFTTDSAACLRGIEIEADV
VLKATKVDGVFTADPVSNPDAELYDKLSYSSVLEKELKVMDLAAFTLARDHNMPIRVFNMNKPGALRRVVMGETEGTLIS
NAE

Sequences:

>Translated_243_residues
MTTNPKPVYQRILLKLSGEALQGEEGFGIDPATLDRMAQEVKELIELGVQVGVVIGGGNLFRGAGLAEAGMNRVVGDHMG
MLATVMNGLAMRDALHRAYVNARVMSAIPLKGVCDDYNWADAIAQLRQGRVVIFSAGTGNPFFTTDSAACLRGIEIEADV
VLKATKVDGVFTADPVSNPDAELYDKLSYSSVLEKELKVMDLAAFTLARDHNMPIRVFNMNKPGALRRVVMGETEGTLIS
NAE
>Mature_242_residues
TTNPKPVYQRILLKLSGEALQGEEGFGIDPATLDRMAQEVKELIELGVQVGVVIGGGNLFRGAGLAEAGMNRVVGDHMGM
LATVMNGLAMRDALHRAYVNARVMSAIPLKGVCDDYNWADAIAQLRQGRVVIFSAGTGNPFFTTDSAACLRGIEIEADVV
LKATKVDGVFTADPVSNPDAELYDKLSYSSVLEKELKVMDLAAFTLARDHNMPIRVFNMNKPGALRRVVMGETEGTLISN
AE

Specific function: Catalyzes the reversible phosphorylation of UMP to UDP

COG id: COG0528

COG function: function code F; Uridylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UMP kinase family

Homologues:

Organism=Escherichia coli, GI1786367, Length=240, Percent_Identity=85.4166666666667, Blast_Score=430, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRH_VIBF1 (Q5E3E1)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205343.1
- HSSP:   P0A7E9
- ProteinModelPortal:   Q5E3E1
- SMR:   Q5E3E1
- STRING:   Q5E3E1
- GeneID:   3278533
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1960
- NMPDR:   fig|312309.3.peg.1960
- eggNOG:   COG0528
- HOGENOM:   HBG497552
- OMA:   RHMEKGR
- PhylomeDB:   Q5E3E1
- ProtClustDB:   PRK00358
- BioCyc:   VFIS312309:VF1960-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01220_B
- InterPro:   IPR001048
- InterPro:   IPR011817
- InterPro:   IPR015963
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF005650
- TIGRFAMs:   TIGR02075

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.4.22

Molecular weight: Translated: 26159; Mature: 26027

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: BINDING 57-57 BINDING 58-58 BINDING 62-62 BINDING 77-77 BINDING 165-165 BINDING 171-171 BINDING 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNPKPVYQRILLKLSGEALQGEEGFGIDPATLDRMAQEVKELIELGVQVGVVIGGGNL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCE
FRGAGLAEAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNARVMSAIPLKGVCDDYNWA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
DAIAQLRQGRVVIFSAGTGNPFFTTDSAACLRGIEIEADVVLKATKVDGVFTADPVSNPD
HHHHHHHCCCEEEEECCCCCCCEECCHHHHHHCCEECCEEEEEEEEECCEEEECCCCCCC
AELYDKLSYSSVLEKELKVMDLAAFTLARDHNMPIRVFNMNKPGALRRVVMGETEGTLIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHEEECCCCCCEEC
NAE
CCC
>Mature Secondary Structure 
TTNPKPVYQRILLKLSGEALQGEEGFGIDPATLDRMAQEVKELIELGVQVGVVIGGGNL
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCE
FRGAGLAEAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNARVMSAIPLKGVCDDYNWA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
DAIAQLRQGRVVIFSAGTGNPFFTTDSAACLRGIEIEADVVLKATKVDGVFTADPVSNPD
HHHHHHHCCCEEEEECCCCCCCEECCHHHHHHCCEECCEEEEEEEEECCEEEECCCCCCC
AELYDKLSYSSVLEKELKVMDLAAFTLARDHNMPIRVFNMNKPGALRRVVMGETEGTLIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHEEECCCCCCEEC
NAE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA