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The map label for this gene is cheB

Identifier: 59712437

GI number: 59712437

Start: 2061976

End: 2063106

Strand: Reverse

Name: cheB

Synonym: VF_1830

Alternate gene names: 59712437

Gene position: 2063106-2061976 (Counterclockwise)

Preceding gene: 59712438

Following gene: 59712436

Centisome position: 71.2

GC content: 43.06

Gene sequence:

>1131_bases
ATGGCTATAAAAGTATTAGTGGTAGATGATTCTAGTTTTTTCCGCCGCCGAGTAAGTGAAATTATCAATTCAGATCCACG
TCTTGAAGTTATCGACGTGGCTGTAAATGGTAAAGAAGCGGTAGAAAAAGCGAAATCATTAAAGCCAGATGTAATAACAA
TGGATATTGAGATGCCAGTCATGGACGGCATCTCAGCAGTCCGAGAGATCATGAAGGCGTGTCCAACACCAACCTTAATG
TTCTCTTCGCTAACTCACGATGGCGCAAAAGCAACACTTGATGCTTTGGATGCGGGTGCTCTTGATTTCTTACCTAAGAA
GTTTGAAGACATTGCTCGTAATCGTGATGAAGCGGTTTCTTTACTACAGAAACGAATTGCTGAAATTGCGCGTAAAAAAT
CATTTATGCGCAGACCAGTACTGAGTTCACAATCAACAAGTACTGTTGAATCTCGTACAGCAACAACACGAACTGCGACT
TCTGCATTAACAGCATCTCCTGTAAAAGCAGCACCAGCAGCTCCAATTGCACAACGCTTTAAAGCGACGGGTAAAAAGTA
TCAATTAACGGCTATTGGGACTTCAACGGGTGGTCCAGTTGCACTGCAAAAGATATTAACGGCGATTCCGGCGAATTACC
CACATCCAATTATTTTGGTTCAACATATGCCTGCCACTTTTACGGCGGCATTTGCTGCTCGTTTAAATAACTTGTGCAAA
ATTCAAGTTAAAGAAGCAGAAGATGGCGATGTGTTAAAACCAGGTTGTGCATATTTGGCTCCAGGTGGTTTGCAAATGAT
GGTTGATGGCCGTCCGGGCGCTTCACGCTTGAAGATTTTAGATGGTGGTGAACGCATGAATTATAAACCATGTGTTGATG
TTACCTTCGGATCTGCTGCGAAAGTATACGGTGATAAAGTTCTGTCGATGATCTTAACTGGTATGGGCGCAGATGGTCGA
GAAGGCTGTCGAATGCTTAAAGCTGGTGGCGCAACTATTTGGTCTCAAGATGAGCAAAGCTGTGTGGTATATGGTATGCC
TCAAGCTGTCGATAAAGCCGGTTTATCTTCAGAAAGTTTACCGCTTGATCGTATTGCTGAAAGAATGCTTGTTGAAGTTG
GATTGAAGTAG

Upstream 100 bases:

>100_bases
TGCAACGATTACTTCTGATGGTCATATTGCATTGATCCTTGACGTTCCTAACTTGTTAAAGCGTTACGCCGTTCGCATGC
GTTAATGCACAAGGATTGAC

Downstream 100 bases:

>100_bases
GAAATAACATGATTGTCTGGAGCATAGCTAATCAGAAAGGTGGCGTGGGTAAAACCACAACCACAATCACATTAGCAGGG
TTGTTAAATCAACGTGGTAA

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAIKVLVVDDSSFFRRRVSEIINSDPRLEVIDVAVNGKEAVEKAKSLKPDVITMDIEMPVMDGISAVREIMKACPTPTLM
FSSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVSLLQKRIAEIARKKSFMRRPVLSSQSTSTVESRTATTRTAT
SALTASPVKAAPAAPIAQRFKATGKKYQLTAIGTSTGGPVALQKILTAIPANYPHPIILVQHMPATFTAAFAARLNNLCK
IQVKEAEDGDVLKPGCAYLAPGGLQMMVDGRPGASRLKILDGGERMNYKPCVDVTFGSAAKVYGDKVLSMILTGMGADGR
EGCRMLKAGGATIWSQDEQSCVVYGMPQAVDKAGLSSESLPLDRIAERMLVEVGLK

Sequences:

>Translated_376_residues
MAIKVLVVDDSSFFRRRVSEIINSDPRLEVIDVAVNGKEAVEKAKSLKPDVITMDIEMPVMDGISAVREIMKACPTPTLM
FSSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVSLLQKRIAEIARKKSFMRRPVLSSQSTSTVESRTATTRTAT
SALTASPVKAAPAAPIAQRFKATGKKYQLTAIGTSTGGPVALQKILTAIPANYPHPIILVQHMPATFTAAFAARLNNLCK
IQVKEAEDGDVLKPGCAYLAPGGLQMMVDGRPGASRLKILDGGERMNYKPCVDVTFGSAAKVYGDKVLSMILTGMGADGR
EGCRMLKAGGATIWSQDEQSCVVYGMPQAVDKAGLSSESLPLDRIAERMLVEVGLK
>Mature_375_residues
AIKVLVVDDSSFFRRRVSEIINSDPRLEVIDVAVNGKEAVEKAKSLKPDVITMDIEMPVMDGISAVREIMKACPTPTLMF
SSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVSLLQKRIAEIARKKSFMRRPVLSSQSTSTVESRTATTRTATS
ALTASPVKAAPAAPIAQRFKATGKKYQLTAIGTSTGGPVALQKILTAIPANYPHPIILVQHMPATFTAAFAARLNNLCKI
QVKEAEDGDVLKPGCAYLAPGGLQMMVDGRPGASRLKILDGGERMNYKPCVDVTFGSAAKVYGDKVLSMILTGMGADGRE
GCRMLKAGGATIWSQDEQSCVVYGMPQAVDKAGLSSESLPLDRIAERMLVEVGLK

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=372, Percent_Identity=36.2903225806452, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_VIBF1 (Q5E3S1)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205213.1
- ProteinModelPortal:   Q5E3S1
- SMR:   Q5E3S1
- STRING:   Q5E3S1
- GeneID:   3279059
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1830
- NMPDR:   fig|312309.3.peg.1829
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   KMAAELN
- PhylomeDB:   Q5E3S1
- ProtClustDB:   PRK00742
- BioCyc:   VFIS312309:VF1830-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 40285; Mature: 40154

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 195-195 ACT_SITE 222-222 ACT_SITE 318-318

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKVLVVDDSSFFRRRVSEIINSDPRLEVIDVAVNGKEAVEKAKSLKPDVITMDIEMPV
CEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHCCCCCEEEEEECCCH
MDGISAVREIMKACPTPTLMFSSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVS
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHH
LLQKRIAEIARKKSFMRRPVLSSQSTSTVESRTATTRTATSALTASPVKAAPAAPIAQRF
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
KATGKKYQLTAIGTSTGGPVALQKILTAIPANYPHPIILVQHMPATFTAAFAARLNNLCK
HHCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCEEE
IQVKEAEDGDVLKPGCAYLAPGGLQMMVDGRPGASRLKILDGGERMNYKPCVDVTFGSAA
EEEEECCCCCCCCCCCCEECCCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEECCCHH
KVYGDKVLSMILTGMGADGREGCRMLKAGGATIWSQDEQSCVVYGMPQAVDKAGLSSESL
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEECCCCCCEEEEECCHHHHHCCCCCCCC
PLDRIAERMLVEVGLK
CHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIKVLVVDDSSFFRRRVSEIINSDPRLEVIDVAVNGKEAVEKAKSLKPDVITMDIEMPV
EEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHCCCCCEEEEEECCCH
MDGISAVREIMKACPTPTLMFSSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVS
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHH
LLQKRIAEIARKKSFMRRPVLSSQSTSTVESRTATTRTATSALTASPVKAAPAAPIAQRF
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
KATGKKYQLTAIGTSTGGPVALQKILTAIPANYPHPIILVQHMPATFTAAFAARLNNLCK
HHCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCEEE
IQVKEAEDGDVLKPGCAYLAPGGLQMMVDGRPGASRLKILDGGERMNYKPCVDVTFGSAA
EEEEECCCCCCCCCCCCEECCCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEECCCHH
KVYGDKVLSMILTGMGADGREGCRMLKAGGATIWSQDEQSCVVYGMPQAVDKAGLSSESL
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEECCCCCCEEEEECCHHHHHCCCCCCCC
PLDRIAERMLVEVGLK
CHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA