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The map label for this gene is ccmE

Identifier: 59712427

GI number: 59712427

Start: 2056087

End: 2056572

Strand: Reverse

Name: ccmE

Synonym: VF_1820

Alternate gene names: 59712427

Gene position: 2056572-2056087 (Counterclockwise)

Preceding gene: 59712428

Following gene: 59712426

Centisome position: 70.98

GC content: 41.56

Gene sequence:

>486_bases
ATGAATCCGAGACGTAAAAAGCGCCTAGGATTGATCCTTGCTCTATTTGTAGGGATCAGTGCAACAGTGGGATTAATGTT
GTATGCATTAAATCAAAACATGGACTTGTTTTATACCCCAACGGAATTAGTTAACGGTAAACCAGACGGTACAAAACCTG
AGGTTGGACAACGTCTACGCATTGGTGGGATGGTTGTAGCCGGTTCTGTTGTACGTGATAATAATTCTCTTGAAGTGTCA
TTTAAGGTTGCCGATGTAGGCCCTCAAGTGACCGTAATTTATGATGGGATTCTTCCTGATTTATTCCGTGAAGGCCAAGG
TATTGTTGCTCAAGGTGTTCTAGTTGATGCAACAACAATTAAAGCTCACGAAGTGTTAGCAAAACACGATGAAGAATACA
TGCCACCAGAAGTTGCAGAAGCGATGAAAAAGACACATGAACCGCTTCAGTATACTGAACAACAAAAACAAGGAACGGGT
CAATGA

Upstream 100 bases:

>100_bases
TTTCTAGCTTAGGCAAACGTCGTCAATTGCTAAAAGAGATTGAGCAAAAAATGGCACGAGAAGAACGAATTAAAAAAGCA
AAAACTATGGAGAATACACT

Downstream 100 bases:

>100_bases
TCGCAGAACTTGGTCACTTTGCAATGATTGCTTCATTAGGGCTTGCCGTCCTTTTGAGCATATTACCTATGTATGGCGCA
AGCCGTGGTAATAAAGCATT

Product: cytochrome c-type biogenesis protein CcmE

Products: NA

Alternate protein names: Cytochrome c maturation protein E; Heme chaperone CcmE

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MNPRRKKRLGLILALFVGISATVGLMLYALNQNMDLFYTPTELVNGKPDGTKPEVGQRLRIGGMVVAGSVVRDNNSLEVS
FKVADVGPQVTVIYDGILPDLFREGQGIVAQGVLVDATTIKAHEVLAKHDEEYMPPEVAEAMKKTHEPLQYTEQQKQGTG
Q

Sequences:

>Translated_161_residues
MNPRRKKRLGLILALFVGISATVGLMLYALNQNMDLFYTPTELVNGKPDGTKPEVGQRLRIGGMVVAGSVVRDNNSLEVS
FKVADVGPQVTVIYDGILPDLFREGQGIVAQGVLVDATTIKAHEVLAKHDEEYMPPEVAEAMKKTHEPLQYTEQQKQGTG
Q
>Mature_161_residues
MNPRRKKRLGLILALFVGISATVGLMLYALNQNMDLFYTPTELVNGKPDGTKPEVGQRLRIGGMVVAGSVVRDNNSLEVS
FKVADVGPQVTVIYDGILPDLFREGQGIVAQGVLVDATTIKAHEVLAKHDEEYMPPEVAEAMKKTHEPLQYTEQQKQGTG
Q

Specific function: Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH

COG id: COG2332

COG function: function code O; Cytochrome c-type biogenesis protein CcmE

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmE/CycJ family

Homologues:

Organism=Escherichia coli, GI1788525, Length=147, Percent_Identity=58.5034013605442, Blast_Score=171, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCME_VIBF1 (Q5E3T1)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205203.1
- ProteinModelPortal:   Q5E3T1
- SMR:   Q5E3T1
- STRING:   Q5E3T1
- GeneID:   3279019
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1820
- NMPDR:   fig|312309.3.peg.1819
- eggNOG:   COG2332
- HOGENOM:   HBG296631
- OMA:   HVEFAVH
- PhylomeDB:   Q5E3T1
- ProtClustDB:   PRK13165
- BioCyc:   VFIS312309:VF1820-MONOMER
- HAMAP:   MF_01959
- InterPro:   IPR004329
- InterPro:   IPR012340
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF03100 CcmE; SSF82093 Pyrv/PenolPyrv_Kinase_cat

EC number: NA

Molecular weight: Translated: 17619; Mature: 17619

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: BINDING 129-129

Signals:

None

Transmembrane regions:

HASH(0x11891d50)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPRRKKRLGLILALFVGISATVGLMLYALNQNMDLFYTPTELVNGKPDGTKPEVGQRLR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCCCCEEE
IGGMVVAGSVVRDNNSLEVSFKVADVGPQVTVIYDGILPDLFREGQGIVAQGVLVDATTI
ECCEEEECEEEECCCCEEEEEEEECCCCEEEEEECCCCHHHHHCCCCEEECCEEEEEHHH
KAHEVLAKHDEEYMPPEVAEAMKKTHEPLQYTEQQKQGTGQ
HHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCH
>Mature Secondary Structure
MNPRRKKRLGLILALFVGISATVGLMLYALNQNMDLFYTPTELVNGKPDGTKPEVGQRLR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCCCCEEE
IGGMVVAGSVVRDNNSLEVSFKVADVGPQVTVIYDGILPDLFREGQGIVAQGVLVDATTI
ECCEEEECEEEECCCCEEEEEEEECCCCEEEEEECCCCHHHHHCCCCEEECCEEEEEHHH
KAHEVLAKHDEEYMPPEVAEAMKKTHEPLQYTEQQKQGTGQ
HHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA