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The map label for this gene is tyrS [H]

Identifier: 59712334

GI number: 59712334

Start: 1949743

End: 1951023

Strand: Reverse

Name: tyrS [H]

Synonym: VF_1727

Alternate gene names: 59712334

Gene position: 1951023-1949743 (Counterclockwise)

Preceding gene: 172087724

Following gene: 59712330

Centisome position: 67.33

GC content: 40.12

Gene sequence:

>1281_bases
ATGACAGCAACAAACGAATTATTGCAAGACTTAAAAGCTCGTGGGCTTATTGCACAGTGCACAGCAGACGAAGAATTAGC
TGAGCACCTTTCAACTGACTGTCGTACTCTCTATTGTGGCTTCGATCCGACAGCAGACAGCTTACATATTGGTAGCCTAG
TACCACTATTAGTTCTTAAGCGTTTCCAACAAGCTGGTCATAAACCATTAGCTCTTGTTGGTGGTGCTACAGGCTTAATC
GGTGACCCAAGTTTTAAAGCTGCTGAGCGTCAACTAAACACGAATGAAGTGGTTGGCGATTGGGTTAACAAAATTAAAGC
ACAAGTTTCAGCTTTTGTTGATTTTACAGAAGAGAAAAATGGTGCTGAAGTAGTAAACAACTTAGATTGGATCGGTCAAA
TTAACGTAATTGAATTTATGCGTGATGTAGGTAAACACTTCAGCGTAAATGCAATGATCCAAAAAGAATCAGTAAAACAA
CGTATCGATCGTGAAGGTTCTGGTATTTCATTTACTGAATTCAGCTACATGCTACTTCAATCATACGATTTTGCAGCATT
GAACAAAGCAAAAGAGTGTACATTACAAATTGGTGGCTCAGATCAGTGGGGTAACATCACTGGTGGTATCGACCTTACTC
GTCGTATGAACCGCAATAAAGTATTTGGTTTAACACTTCCTCTAGTTACAAAATCAGATGGTACTAAGTTTGGTAAAACT
GAATCAGGTACAATTTGGTTAGATTCAAATAAAACATCACCATACGCTTTCTATCAATTCTGGTTAGGTACTGCGGATGC
TGACGTATATAACTTCCTACGTTTCTTCACTTTCTTAACAGTAGAAGAGATTGCAGCTGTTGAAGAGAGTGATAAGAGTG
TTCAGGGCCGTCCTGAAGGTCAAGGCATCCTCGCTCGTGAAGTAACTCGTTTAGTTCACGGTGAAGAAGGTTTAGCGTCT
GCTGAACGTATCACGAAAGCGCTTTTCTCTGGTGATCTTTCTTCACTAACAGAAACTGACTTAGCACAACTTGCTTTAGA
TGGCCTGCCATCAACAGAATTAGAAGCATCTGAACAAACGATTGTTGAAGTTCTAACTCAATCTGAATTAGCTAAATCAA
ACAAAATGGCGCGTGAATTCATCGGTAACGGTGCAGTATCGGTAAACGGCGAGAAAGTTGCTGATACCGAAGCGGTACTT
AAGAAAGAAGATGCACTGTTTGGTAAATACAGCGTGATCAAACGCGGTAAGAAGCTATTTAACCTATACATCTGGAAATA
A

Upstream 100 bases:

>100_bases
TTGAAGTATTTTTACTCGTAAACTCTGTTATTTAGCCGCTAAATGTGTAAAATCGAGTATTCTTTTTTATACCTAAATTT
AGAGTACAAGTAGGTTATCT

Downstream 100 bases:

>100_bases
GCATTCCATCGTGCTGGAGTAGGGAAATAATTCTCTAGTCCATCATAGTATGATTATTATTTGAGATAATAAAAAGCGAA
CCTCATTATTGGGTTCGCTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTATNELLQDLKARGLIAQCTADEELAEHLSTDCRTLYCGFDPTADSLHIGSLVPLLVLKRFQQAGHKPLALVGGATGLI
GDPSFKAAERQLNTNEVVGDWVNKIKAQVSAFVDFTEEKNGAEVVNNLDWIGQINVIEFMRDVGKHFSVNAMIQKESVKQ
RIDREGSGISFTEFSYMLLQSYDFAALNKAKECTLQIGGSDQWGNITGGIDLTRRMNRNKVFGLTLPLVTKSDGTKFGKT
ESGTIWLDSNKTSPYAFYQFWLGTADADVYNFLRFFTFLTVEEIAAVEESDKSVQGRPEGQGILAREVTRLVHGEEGLAS
AERITKALFSGDLSSLTETDLAQLALDGLPSTELEASEQTIVEVLTQSELAKSNKMAREFIGNGAVSVNGEKVADTEAVL
KKEDALFGKYSVIKRGKKLFNLYIWK

Sequences:

>Translated_426_residues
MTATNELLQDLKARGLIAQCTADEELAEHLSTDCRTLYCGFDPTADSLHIGSLVPLLVLKRFQQAGHKPLALVGGATGLI
GDPSFKAAERQLNTNEVVGDWVNKIKAQVSAFVDFTEEKNGAEVVNNLDWIGQINVIEFMRDVGKHFSVNAMIQKESVKQ
RIDREGSGISFTEFSYMLLQSYDFAALNKAKECTLQIGGSDQWGNITGGIDLTRRMNRNKVFGLTLPLVTKSDGTKFGKT
ESGTIWLDSNKTSPYAFYQFWLGTADADVYNFLRFFTFLTVEEIAAVEESDKSVQGRPEGQGILAREVTRLVHGEEGLAS
AERITKALFSGDLSSLTETDLAQLALDGLPSTELEASEQTIVEVLTQSELAKSNKMAREFIGNGAVSVNGEKVADTEAVL
KKEDALFGKYSVIKRGKKLFNLYIWK
>Mature_425_residues
TATNELLQDLKARGLIAQCTADEELAEHLSTDCRTLYCGFDPTADSLHIGSLVPLLVLKRFQQAGHKPLALVGGATGLIG
DPSFKAAERQLNTNEVVGDWVNKIKAQVSAFVDFTEEKNGAEVVNNLDWIGQINVIEFMRDVGKHFSVNAMIQKESVKQR
IDREGSGISFTEFSYMLLQSYDFAALNKAKECTLQIGGSDQWGNITGGIDLTRRMNRNKVFGLTLPLVTKSDGTKFGKTE
SGTIWLDSNKTSPYAFYQFWLGTADADVYNFLRFFTFLTVEEIAAVEESDKSVQGRPEGQGILAREVTRLVHGEEGLASA
ERITKALFSGDLSSLTETDLAQLALDGLPSTELEASEQTIVEVLTQSELAKSNKMAREFIGNGAVSVNGEKVADTEAVLK
KEDALFGKYSVIKRGKKLFNLYIWK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=438, Percent_Identity=34.703196347032, Blast_Score=238, Evalue=6e-63,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=62.2641509433962, Blast_Score=559, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI25145802, Length=404, Percent_Identity=36.6336633663366, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI25145805, Length=279, Percent_Identity=35.84229390681, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6325160, Length=371, Percent_Identity=37.466307277628, Blast_Score=248, Evalue=9e-67,
Organism=Drosophila melanogaster, GI20130383, Length=438, Percent_Identity=36.5296803652968, Blast_Score=281, Evalue=8e-76,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46881; Mature: 46749

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATNELLQDLKARGLIAQCTADEELAEHLSTDCRTLYCGFDPTADSLHIGSLVPLLVLK
CCCHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHH
RFQQAGHKPLALVGGATGLIGDPSFKAAERQLNTNEVVGDWVNKIKAQVSAFVDFTEEKN
HHHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GAEVVNNLDWIGQINVIEFMRDVGKHFSVNAMIQKESVKQRIDREGSGISFTEFSYMLLQ
CHHHHHCCHHCCCHHHHHHHHHHCCCCEEHHEEHHHHHHHHHCCCCCCCCHHHHHHHHHH
SYDFAALNKAKECTLQIGGSDQWGNITGGIDLTRRMNRNKVFGLTLPLVTKSDGTKFGKT
CCCHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEEEEEEECCCCCCCCCC
ESGTIWLDSNKTSPYAFYQFWLGTADADVYNFLRFFTFLTVEEIAAVEESDKSVQGRPEG
CCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QGILAREVTRLVHGEEGLASAERITKALFSGDLSSLTETDLAQLALDGLPSTELEASEQT
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
IVEVLTQSELAKSNKMAREFIGNGAVSVNGEKVADTEAVLKKEDALFGKYSVIKRGKKLF
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NLYIWK
EEEECC
>Mature Secondary Structure 
TATNELLQDLKARGLIAQCTADEELAEHLSTDCRTLYCGFDPTADSLHIGSLVPLLVLK
CCHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHH
RFQQAGHKPLALVGGATGLIGDPSFKAAERQLNTNEVVGDWVNKIKAQVSAFVDFTEEKN
HHHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GAEVVNNLDWIGQINVIEFMRDVGKHFSVNAMIQKESVKQRIDREGSGISFTEFSYMLLQ
CHHHHHCCHHCCCHHHHHHHHHHCCCCEEHHEEHHHHHHHHHCCCCCCCCHHHHHHHHHH
SYDFAALNKAKECTLQIGGSDQWGNITGGIDLTRRMNRNKVFGLTLPLVTKSDGTKFGKT
CCCHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEEEEEEECCCCCCCCCC
ESGTIWLDSNKTSPYAFYQFWLGTADADVYNFLRFFTFLTVEEIAAVEESDKSVQGRPEG
CCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QGILAREVTRLVHGEEGLASAERITKALFSGDLSSLTETDLAQLALDGLPSTELEASEQT
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
IVEVLTQSELAKSNKMAREFIGNGAVSVNGEKVADTEAVLKKEDALFGKYSVIKRGKKLF
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NLYIWK
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA