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The map label for this gene is metE

Identifier: 59712328

GI number: 59712328

Start: 1939006

End: 1941330

Strand: Reverse

Name: metE

Synonym: VF_1721

Alternate gene names: 59712328

Gene position: 1941330-1939006 (Counterclockwise)

Preceding gene: 172087723

Following gene: 59712326

Centisome position: 67.0

GC content: 41.59

Gene sequence:

>2325_bases
ATGACGACAGAATCGAAGACAAAGACAGTAACTCATATTTTAGGTTATCCGCGCGTTGGTGCTCAGAGAGAATTAAAATT
TGCTCAGGAAAAATATTGGCGCGGTGAGATAGAACAGAAAGAACTATTGGCTGTAGGCAGTGAATTACGACAACGTCACT
GGAAAGATCAATCCGCTTCAGGACTGGATTTTGTGACTGCAGGTGATTTTGCTTGGTACGATCATGTATTAACCACAAGT
TTACTCTTAGGACATGTGCCAGCTCGTCATAATAACGGTTTCCCTGATTTAGATACGTTATTTAAAGTAGGGCGTGGTCA
ATCTCAAAACAGCTGTGGTTGTGGTGAAGCCGCATCGGATATGACCAAGTGGTTTAATACCAATTATCACTACATCGTGC
CGGAATTTTCTAAAAACGATAAATTTAATGTCAGTTGGTCACAACTTTTTGATGAGATTGCAGAGGCACAAAAGCAAGGA
CATAATGTTAAGCCTGTTTTATTAGGCCCACTTAGCTACCTTTGGTTAGGTAAAGAAGTGAATGATGAAGAGGTTGAACA
AGGGTTTGATCGTTTATCATTATTGCCTCGTCTATTGACCGCTTATCAAGCTATTTTCTCTAAGCTTAGTGCGTTAGGTG
TTGAATGGGTTCAAATTGATGAGCCAATTCTCGCATTAGAATTACCAAAAGCATGGGCGGATTCTTTTAAATTAGCTTAT
CAAGTGATTCAAAGTGATGTGAAATTACTCTTAACCACCTATTTTGATGGCGTAGAGCATCATTTAGATAAAATTACAAA
ACTTGCAGTAAATGGTTTACATATTGATGTTTCTGCGGCCCCTGACCAGTTAGATGCGATTGTGAGTGCGTTACCAAAAG
AGTGGGTATTATCCGTTGGTGCCGTTAATGGCCGTAATGTATGGCGTGCCGATTTAGAACGTTTGCATGAAAGGCTGCAA
CCAGTAAAAGAAGCGTTGGGTGATAAATTATGGATCGCAAGTTCATGTTCATTATTGCATAGCCCCGTCGATTTAGATCA
AGAGACGGAACTATCAAAAGAAACACTGCAATGGTTTGCGTTTGCTAAGCAAAAATTAAGAGAGGTGACGCTGCTAGCTG
ATGCTTTAGATGGTAATCAAAATGCGATTTTAGCATGTCATCAATACAGTCAACCACTGCGTGAGCGTGAGAGTGCGGAG
CATATTAATAAGCCAGCAGTACAGCAACGTTTAGCTGCTATTACGCCAGCGTTAGCCGAGCGTGCAGAAGCGTATTCTGT
TCGAGCACAGCATCAAGCTGAAAAGTTGGGCTTACCATTATTACCAACAACAACAATTGGTTCATTCCCACAAACTTCTG
ATATTCGTCAACAACGCAGTGCCTACCGTACAGGTAAATTGAACGAGCAAGATTATGTTACTGCAATGAAAGGACATATT
GCTGATGCAGTAGAGCGTCAAGAACGTTTAGATTTGGATGTACTCGTGCATGGTGAGGCAGAGCGTAATGACATGGTTGA
ATACTTTGCGGAAAATCTAAATGGATTTCAAGCAACACGATTTGGCTGGGTACAAAGTTATGGTTCTCGTTGTGTAAAGC
CGGCAATTGTGGTGGCTGATATTGAACGTGAAGCACCTATTACAGTGAGCTGGACACAGTACGCTCAGTCGTTAACAACC
AAACAAATGAAAGGAATGCTAACAGGACCTGTCACTATTCTTGGATGGACGTTCCCACGTGAAGACATTACACGTAAAGA
AATCGCACAGCAGTTAGCTTTGGTGTTGCGTGATGAGGTTTCAGATCTTCAGCAAGCGGGCATTAATATTATTCAAATTG
ATGAGCCAGCTATTCGTGAAGGACTACCCATTAAAAAGAGCGATCAAAAAGCGTATTTAGATTGGGCAGTTGAAGCCTTT
AAGATTTCAGCGGCCAGTGCTAAGTCGGAGACACAAATTCATACTCATATGTGTTATAGCGAGTTTAATGAAATTATTGA
ATCGGTGGCAGCGCTAGATGCAGATGTGATTACCATTGAAACGTCTCGTTCAAATATGGAGTTATTGAAAGCGTTTGAAG
ACTTCAATTATCCAAATGAAATTGGACCGGGTGTGTATGATATCCACTCCCCGAATATTCCAAGCCAAGAGTGGATTGTT
GACCTCATAGAAACGGCAGCAGCAAGAGTGCCAGTAGAGCGTTTATGGGTGAATCCGGATTGTGGTCTGAAAACTCGAAA
TTGGAAAGAAACAGAAGCGGCGCTTGAAAATATGGTAAAAGCAGCAAAGGCACTGCGTAAGAAGTGGCAATCAAACGCGG
CTTAA

Upstream 100 bases:

>100_bases
GTTGAATATAATTCATAATCAAAGTGAAAGATTAGAAATTGTACAGTGAGAAAAAAAACGTCATTGTTTAGCCATCCAAA
CGGCTAAAGGAATTAGACAA

Downstream 100 bases:

>100_bases
TTTGCAATAATATATTTTTAAACAAGAGCGAAGCCCATCATCAGCTTCGCTTTTTTATTTCTGCTATCGAGTCGTAACCC
AAAGAGCGTGGACCATTCCT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 774; Mature: 773

Protein sequence:

>774_residues
MTTESKTKTVTHILGYPRVGAQRELKFAQEKYWRGEIEQKELLAVGSELRQRHWKDQSASGLDFVTAGDFAWYDHVLTTS
LLLGHVPARHNNGFPDLDTLFKVGRGQSQNSCGCGEAASDMTKWFNTNYHYIVPEFSKNDKFNVSWSQLFDEIAEAQKQG
HNVKPVLLGPLSYLWLGKEVNDEEVEQGFDRLSLLPRLLTAYQAIFSKLSALGVEWVQIDEPILALELPKAWADSFKLAY
QVIQSDVKLLLTTYFDGVEHHLDKITKLAVNGLHIDVSAAPDQLDAIVSALPKEWVLSVGAVNGRNVWRADLERLHERLQ
PVKEALGDKLWIASSCSLLHSPVDLDQETELSKETLQWFAFAKQKLREVTLLADALDGNQNAILACHQYSQPLRERESAE
HINKPAVQQRLAAITPALAERAEAYSVRAQHQAEKLGLPLLPTTTIGSFPQTSDIRQQRSAYRTGKLNEQDYVTAMKGHI
ADAVERQERLDLDVLVHGEAERNDMVEYFAENLNGFQATRFGWVQSYGSRCVKPAIVVADIEREAPITVSWTQYAQSLTT
KQMKGMLTGPVTILGWTFPREDITRKEIAQQLALVLRDEVSDLQQAGINIIQIDEPAIREGLPIKKSDQKAYLDWAVEAF
KISAASAKSETQIHTHMCYSEFNEIIESVAALDADVITIETSRSNMELLKAFEDFNYPNEIGPGVYDIHSPNIPSQEWIV
DLIETAAARVPVERLWVNPDCGLKTRNWKETEAALENMVKAAKALRKKWQSNAA

Sequences:

>Translated_774_residues
MTTESKTKTVTHILGYPRVGAQRELKFAQEKYWRGEIEQKELLAVGSELRQRHWKDQSASGLDFVTAGDFAWYDHVLTTS
LLLGHVPARHNNGFPDLDTLFKVGRGQSQNSCGCGEAASDMTKWFNTNYHYIVPEFSKNDKFNVSWSQLFDEIAEAQKQG
HNVKPVLLGPLSYLWLGKEVNDEEVEQGFDRLSLLPRLLTAYQAIFSKLSALGVEWVQIDEPILALELPKAWADSFKLAY
QVIQSDVKLLLTTYFDGVEHHLDKITKLAVNGLHIDVSAAPDQLDAIVSALPKEWVLSVGAVNGRNVWRADLERLHERLQ
PVKEALGDKLWIASSCSLLHSPVDLDQETELSKETLQWFAFAKQKLREVTLLADALDGNQNAILACHQYSQPLRERESAE
HINKPAVQQRLAAITPALAERAEAYSVRAQHQAEKLGLPLLPTTTIGSFPQTSDIRQQRSAYRTGKLNEQDYVTAMKGHI
ADAVERQERLDLDVLVHGEAERNDMVEYFAENLNGFQATRFGWVQSYGSRCVKPAIVVADIEREAPITVSWTQYAQSLTT
KQMKGMLTGPVTILGWTFPREDITRKEIAQQLALVLRDEVSDLQQAGINIIQIDEPAIREGLPIKKSDQKAYLDWAVEAF
KISAASAKSETQIHTHMCYSEFNEIIESVAALDADVITIETSRSNMELLKAFEDFNYPNEIGPGVYDIHSPNIPSQEWIV
DLIETAAARVPVERLWVNPDCGLKTRNWKETEAALENMVKAAKALRKKWQSNAA
>Mature_773_residues
TTESKTKTVTHILGYPRVGAQRELKFAQEKYWRGEIEQKELLAVGSELRQRHWKDQSASGLDFVTAGDFAWYDHVLTTSL
LLGHVPARHNNGFPDLDTLFKVGRGQSQNSCGCGEAASDMTKWFNTNYHYIVPEFSKNDKFNVSWSQLFDEIAEAQKQGH
NVKPVLLGPLSYLWLGKEVNDEEVEQGFDRLSLLPRLLTAYQAIFSKLSALGVEWVQIDEPILALELPKAWADSFKLAYQ
VIQSDVKLLLTTYFDGVEHHLDKITKLAVNGLHIDVSAAPDQLDAIVSALPKEWVLSVGAVNGRNVWRADLERLHERLQP
VKEALGDKLWIASSCSLLHSPVDLDQETELSKETLQWFAFAKQKLREVTLLADALDGNQNAILACHQYSQPLRERESAEH
INKPAVQQRLAAITPALAERAEAYSVRAQHQAEKLGLPLLPTTTIGSFPQTSDIRQQRSAYRTGKLNEQDYVTAMKGHIA
DAVERQERLDLDVLVHGEAERNDMVEYFAENLNGFQATRFGWVQSYGSRCVKPAIVVADIEREAPITVSWTQYAQSLTTK
QMKGMLTGPVTILGWTFPREDITRKEIAQQLALVLRDEVSDLQQAGINIIQIDEPAIREGLPIKKSDQKAYLDWAVEAFK
ISAASAKSETQIHTHMCYSEFNEIIESVAALDADVITIETSRSNMELLKAFEDFNYPNEIGPGVYDIHSPNIPSQEWIVD
LIETAAARVPVERLWVNPDCGLKTRNWKETEAALENMVKAAKALRKKWQSNAA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=761, Percent_Identity=61.1038107752957, Blast_Score=956, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=770, Percent_Identity=44.8051948051948, Blast_Score=662, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_VIBF1 (Q5E430)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205104.1
- HSSP:   Q9X112
- ProteinModelPortal:   Q5E430
- SMR:   Q5E430
- STRING:   Q5E430
- GeneID:   3278980
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1721
- NMPDR:   fig|312309.3.peg.1721
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- PhylomeDB:   Q5E430
- ProtClustDB:   PRK05222
- BioCyc:   VFIS312309:VF1721-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 87094; Mature: 86963

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTESKTKTVTHILGYPRVGAQRELKFAQEKYWRGEIEQKELLAVGSELRQRHWKDQSAS
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
GLDFVTAGDFAWYDHVLTTSLLLGHVPARHNNGFPDLDTLFKVGRGQSQNSCGCGEAASD
CCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHH
MTKWFNTNYHYIVPEFSKNDKFNVSWSQLFDEIAEAQKQGHNVKPVLLGPLSYLWLGKEV
HHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHCCCCC
NDEEVEQGFDRLSLLPRLLTAYQAIFSKLSALGVEWVQIDEPILALELPKAWADSFKLAY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHH
QVIQSDVKLLLTTYFDGVEHHLDKITKLAVNGLHIDVSAAPDQLDAIVSALPKEWVLSVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCHHHHHHHC
AVNGRNVWRADLERLHERLQPVKEALGDKLWIASSCSLLHSPVDLDQETELSKETLQWFA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHH
FAKQKLREVTLLADALDGNQNAILACHQYSQPLRERESAEHINKPAVQQRLAAITPALAE
HHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RAEAYSVRAQHQAEKLGLPLLPTTTIGSFPQTSDIRQQRSAYRTGKLNEQDYVTAMKGHI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ADAVERQERLDLDVLVHGEAERNDMVEYFAENLNGFQATRFGWVQSYGSRCVKPAIVVAD
HHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEE
IEREAPITVSWTQYAQSLTTKQMKGMLTGPVTILGWTFPREDITRKEIAQQLALVLRDEV
CCCCCCEEEEHHHHHHHHHHHHHHCHHCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHH
SDLQQAGINIIQIDEPAIREGLPIKKSDQKAYLDWAVEAFKISAASAKSETQIHTHMCYS
HHHHHCCCEEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EFNEIIESVAALDADVITIETSRSNMELLKAFEDFNYPNEIGPGVYDIHSPNIPSQEWIV
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHH
DLIETAAARVPVERLWVNPDCGLKTRNWKETEAALENMVKAAKALRKKWQSNAA
HHHHHHHHHCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTESKTKTVTHILGYPRVGAQRELKFAQEKYWRGEIEQKELLAVGSELRQRHWKDQSAS
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
GLDFVTAGDFAWYDHVLTTSLLLGHVPARHNNGFPDLDTLFKVGRGQSQNSCGCGEAASD
CCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHH
MTKWFNTNYHYIVPEFSKNDKFNVSWSQLFDEIAEAQKQGHNVKPVLLGPLSYLWLGKEV
HHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHCCCCC
NDEEVEQGFDRLSLLPRLLTAYQAIFSKLSALGVEWVQIDEPILALELPKAWADSFKLAY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHH
QVIQSDVKLLLTTYFDGVEHHLDKITKLAVNGLHIDVSAAPDQLDAIVSALPKEWVLSVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCHHHHHHHC
AVNGRNVWRADLERLHERLQPVKEALGDKLWIASSCSLLHSPVDLDQETELSKETLQWFA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHH
FAKQKLREVTLLADALDGNQNAILACHQYSQPLRERESAEHINKPAVQQRLAAITPALAE
HHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RAEAYSVRAQHQAEKLGLPLLPTTTIGSFPQTSDIRQQRSAYRTGKLNEQDYVTAMKGHI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ADAVERQERLDLDVLVHGEAERNDMVEYFAENLNGFQATRFGWVQSYGSRCVKPAIVVAD
HHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEE
IEREAPITVSWTQYAQSLTTKQMKGMLTGPVTILGWTFPREDITRKEIAQQLALVLRDEV
CCCCCCEEEEHHHHHHHHHHHHHHCHHCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHH
SDLQQAGINIIQIDEPAIREGLPIKKSDQKAYLDWAVEAFKISAASAKSETQIHTHMCYS
HHHHHCCCEEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EFNEIIESVAALDADVITIETSRSNMELLKAFEDFNYPNEIGPGVYDIHSPNIPSQEWIV
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHH
DLIETAAARVPVERLWVNPDCGLKTRNWKETEAALENMVKAAKALRKKWQSNAA
HHHHHHHHHCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA